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    • 4. 发明专利
    • Method of expression of exogenous gene in plant induced by chemical substance
    • 化学物质诱导植物外源基因表达的方法
    • JP2009050252A
    • 2009-03-12
    • JP2008001027
    • 2008-01-08
    • Sumitomo Chemical Co Ltd住友化学株式会社
    • SAIJO TAKANORINAGASAWA AKITO
    • C12N15/09A01H1/00A01H5/00C12P21/02
    • C12N15/8238C07K2319/71C12N15/8217C12N2770/00011C12N2770/00051C12N2830/30C12N2830/60
    • PROBLEM TO BE SOLVED: To provide a method or the like by which the improvement of induction magnitude in the expression of a target exogenous gene induced in a plant is achieved in a system induced by a copper ion. SOLUTION: The expression method of a target exogenous gene in a plant induced by a chemical substance, comprises a step of activating with copper ions a transcription factor encoded by an exogenous gene different from the target exogenous gene to induce activation of transcription of the target exogenous gene by a region having a promoter function contained in the target exogenous gene, wherein the transcription factor is contained in a gene construct which is constructed so as to comprise a 5'-untranslated region of an arbitrary exogenous gene downstream from the region having a promoter function contained in the target exogenous gene. COPYRIGHT: (C)2009,JPO&INPIT
    • 待解决的问题:提供一种方法,通过该方法等,在由铜离子诱导的系统中实现在植物中诱导的靶外源基因的表达中的诱导量的改善。 解决方案:由化学物质诱导的植物中的靶外源基因的表达方法包括用铜离子激活由与靶外源基因不同的外源基因编码的转录因子以诱导转录活化的步骤 所述目标外源基因具有包含在所述靶外源基因中的启动子功能的区域,其中所述转录因子包含在构建成包含所述区域下游的任意外源基因的5'-非翻译区的基因构建体中 具有包含在靶外源基因中的启动子功能。 版权所有(C)2009,JPO&INPIT