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    • 9. 发明专利
    • Promoter
    • 促销者
    • JPS61111691A
    • 1986-05-29
    • JP23352984
    • 1984-11-06
    • Kirin Brewery Co LtdMarubeni Kk
    • KOMANO TORUOOYAMA KANJI
    • C12N15/09C12N15/82
    • C12N15/82
    • PURPOSE:To prepare a promoter having improved handling property and high activity, from a DNA having the same base sequence as the DNA fragments of 533 bp and 188 bp and prepared by the incision with a restriction enzyme HincII or AluI. CONSTITUTION:The chloroplast of liverwort (Marchantia polymorpha) is incised with a restriction enzyme ECOR1, and the obtained DNA fragment of about 5.1kbp is incised directly with restriction enzyme HincII or AluI to obtain a promoter. Concretely, a DNA fragment of about 5.1kbp is incised with a restriction enzyme BamH1 to obtain a DNA fragment of about 1.1kbp as a precursor promoter. The precursor promoter is again incised with a restriction enzyme HincII to form a DNA fragment of 533 bp as a promoter. The objective DNA of 188 bp and acting as a promoter can be prepared by incising the DNA fragment with a restriction enzyme AluI.
    • 目的:从具有与533bp和188bp的DNA片段相同的碱基序列的DNA制备具有改善的处理性和高活性的启动子,并通过切口用限制酶HincII或AluI制备。 构成:用限制性内切酶ECOR1切割肝叶(Marchantia polymorpha)的叶绿体,用限制酶HincII或AluI直接切割得到的约5.1kbp的DNA片段,得到启动子。 具体地,用限制酶BamH1切割约5.1kbp的DNA片段,得到约1.1kbp的DNA片段作为前体启动子。 用限制酶HincII再次切割前体启动子,形成533bp的DNA片段作为启动子。 188 bp的目标DNA作为启动子可以通过用限制酶AluI切割DNA片段来制备。