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1. 发明专利

JPS6144352A Method for fluorescent marking of polydeoxyribonucleotide or polyribonucleotide 失效
标题翻译：氟代二氧化碳或聚氨酯的荧光标记方法
公开(公告)号：JPS6144352A
公开(公告)日：1986-03-04
申请号：JP16536184
申请日：1984-08-07
申请人： Yuki Gosei Yakuhin Kogyo Kk
发明人： KOBAYASHI MAKOTO , SUGIMOTO NOBUTAKA
IPC分类号： G01N33/50 , C07H21/00 , C07H21/04 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/533
CPC分类号： C12Q1/68
摘要： PURPOSE:To enhance the detection sensitivity of marked polynucleotide by an optical marking method, by introducing an optical marking compound into the thiophosphoric acid group of a nucleic acid constitutional element. CONSTITUTION:The fluorescent marking of polynucleotide is performed by converting the phosphoric acid bond of polynucleotide to a thiophosphoric acid bond and reacting the thiol group of the thiophosphoric acid bond with a covalently bondable fluorescent marking compound specifically reacting said thiol group. The above-mentioned polynucleotide having the thiophosphoric acid bond can be synthesized by an org. synthesis method or enzymatic method. Especially, in the former org. synthesis method, it is easiest to introduce the thiophosphoric group into both terminal hydroxyl groups of polynucleotide and thiophosphoric acid, thiophosphoryl chloride or derivatives thereof may be reacted under a known condition.
摘要翻译：目的：通过光学标记法提高标记多核苷酸的检测灵敏度，通过将光学标记化合物引入核酸结构元件的硫代磷酸基团。构成：多核苷酸的荧光标记通过将多核苷酸的磷酸键转化为硫代磷酸键，并使硫代磷酸键的硫醇基与与所述硫醇基特别反应的共价键合的荧光标记化合物反应来进行。上述具有硫代磷酸键的多核苷酸可以通过组织合成。合成方法或酶法。特别是在前组织。合成方法中，最好将硫代磷酸引入多核苷酸和硫代磷酸的两个末端羟基，硫代磷酰氯或其衍生物可以在已知条件下反应。

2. 发明专利

JPS6144353A Method for fluorescent marking of polydeoxyribonucleotide or polyribonucleotide 失效
标题翻译：氟代二氧化碳或聚氨酯的荧光标记方法
公开(公告)号：JPS6144353A
公开(公告)日：1986-03-04
申请号：JP16536284
申请日：1984-08-07
申请人： Yuki Gosei Yakuhin Kogyo Kk
发明人： KOBAYASHI MAKOTO , SUGIMOTO NOBUTAKA
IPC分类号： G01N33/50 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/00 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/533 , G01N33/58
CPC分类号： C12Q1/68
摘要： PURPOSE:To enhance the detection sensitivity of marked polynucleotide by an optical marking method, by performing the fluorescent marking of phosphonic acid by reacting the functional group of a phosphonic acid group with an optical marking compound. CONSTITUTION:The fluorescent marking of polynucleotide is performed by converting the phosphoric acid group of polynucleotide to a phosphonic acid bond having a functional group represented by formula (wherein R is aliphatic or aromatic hydrocarbon which may have an 1-20C branched chain and X is a functional group which may have a protective group) and reacting said functional group with a covalently bondable fluorescent marking compound. As a reaction method of phosphonic acid and the fluorescent marking compound, there are a method wherein the fluorescent marking compound is directly bonded to a phosphorus atom through a phosphorus-carbon bond and a method wherein the functional group specifically reacted with the fluorescent marking compound is bonded to a phosphorus atom so as to interpose aliphatic or aromatic hydrocarbon, which may have a branched chain, therebetween and the fluorescent marking compound is subsequently reacted with said functional group but, from the aspect of reaction yield or reaction operation, the latter is more excellent.
摘要翻译：目的：通过光学标记方法提高标记多核苷酸的检测灵敏度，通过使膦酸基团的官能团与光学标记化合物反应来进行膦酸的荧光标记。构成：多核苷酸的荧光标记通过将多核苷酸的磷酸基团转换为具有由式（其中R为可具有1-20C支链的脂族或芳族烃，且X为可以具有保护基的官能团），并使所述官能团与共价键合的荧光标记化合物反应。作为膦酸和荧光标记化合物的反应方法，存在荧光标记化合物通过磷 - 碳键直接与磷原子键合的方法，其中与荧光标记化合物特异性反应的官能团为与磷原子键合以便在其间插入可以具有支链的脂族或芳族烃，并且荧光标记化合物随后与所述官能团反应，但是从反应产率或反应操作的方面来看，后者更多优秀。

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