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    • 1. 发明专利
    • Stem and progenitor cell composition recovered from bone marrow or cord blood and system and method for preparing thereof
    • 从骨髓或骨髓中恢复的干细胞和前体细胞组合物及其制备方法
    • JP2014176389A
    • 2014-09-25
    • JP2014112675
    • 2014-05-30
    • Thermogenesis Corpサーモジェネシス コーポレーション
    • PHILIP H COELHOBRUCE A BAKERJOHN R CHAPMANLI JUNZHIROBERT S CHILDERSPRINCE EMMANUEL
    • C12N5/0789C12M3/00
    • C12N5/0647A61K2035/124A61M1/0236A61M1/3693A61M2202/0437A61M2205/331C12N5/0663C12N5/0665
    • PROBLEM TO BE SOLVED: To provide a system and a method capable of recovering stem cells at a high percentage and red blood cells at a low percentage.SOLUTION: The stem cell compositions includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The system for preparing the compositions includes a bag set 100 and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag 106. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve 138 in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.
    • 要解决的问题:提供能够以低百分比以高百分比和红细胞回收干细胞的系统和方法。解决方案:干细胞组合物包括从骨髓或脐血回收的干细胞和祖细胞的组合物 含有大多数可行的CD34 +细胞,并且基本上耗尽了原始样品中的红细胞,没有任何异生素添加剂来辅助细胞分离。 用于制备组合物的系统包括袋组100和处理装置,其利用光学传感器,微控制器,伺服电动机,加速度计,称重传感器和电池。 该系统和方法利用离心将细胞分层,然后将干细胞分离并转移到干细胞袋106中。处理装置的微控制器接收来自装置加速度计,称重传感器和光学传感器的输入,以将计量阀138引导到 袋子打开和关闭以允许尽可能少的红细胞转移尽可能多的茎细胞。