专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2003169661A APPARATUS FOR ANALYZING NUCLEIC ACID AND METHOD FOR ANALYZING NUCLEIC ACID BY USING THE SAME 审中-公开
公开(公告)号：JP2003169661A
公开(公告)日：2003-06-17
申请号：JP2001371937
申请日：2001-12-05
申请人： TOYO BOSEKI , HITACHI MAXELL
发明人： NISHIYA YOSHIAKI , IKEDA KATSUNORI , KISHIMOTO MIKIO , UMEBAYASHI NOBUHIRO
IPC分类号： G01N33/48 , C12M1/00 , C12Q1/68 , G01N27/447 , G01N33/53 , G01N33/533 , G01N33/566 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide an inexpensive, simple and miniaturized apparatus for extracting and refining a nucleic acid from a sample containing the nucleic acid and analyzing the nucleic acid and to provide a method using the apparatus. SOLUTION: This invention provides the apparatus for analyzing the nucleic acid in the sample, having the following means (a) to (d) and the analytic method using the apparatus. (a) A means for bringing the sample into contact with magnetism-responding particles having a ferromagnetic metal oxide and silica and carrying out treatment for absorbing the nucleic acid in the sample into the magnetism-responding particles. (b) A means for carrying out treatment for separating magnetism-responding particles, in which the nucleic acid is absorbed, from the sample by applying magnetic field thereto. (c) A means for carrying out treatment for separating the nucleic acid from the magnetism- responding particles by applying electric field thereto. (d) A means for carrying out treatment for amplifying the nucleic acid separated from the magnetism- responding particles. COPYRIGHT: (C)2003,JPO

2. 发明专利

JP2000333669A PROCESSING MEANS, SAMPLE PROCESSING METHOD AND ITS DEVICE 失效
公开(公告)号：JP2000333669A
公开(公告)日：2000-12-05
申请号：JP14943299
申请日：1999-05-28
申请人： TOYO BOSEKI
发明人： KUSUMOTO MASAHIRO , IKEDA KATSUNORI , NISHIYA YOSHIAKI , KAWAKAMI FUMIKIYO , KAWAMURA YOSHIHISA
IPC分类号： C12M1/33
摘要： PROBLEM TO BE SOLVED: To provide a processing means capable of finely processing, agitating and homogenizing a living body sample derived from a wide range of animals and plants irrespective to its specific gravity either large or small in a good efficiency and useful for the extraction of nucleic acids, testing of pathogenic bacteria, etc., by dividing the tip end part for capable of nipping the samples. SOLUTION: This processing means 10 can process a sample 4 in a container 2 by moving in the container 2, and its tip part 10a is divided to be capable of nipping the sample 4. Further, the tip end part 10a is divided so as to be able to be elastically deformed to be capable of nipping the sample 4 as restrained by the shape of the container 2 or to be separated the more from each other on going the more to the tip end, also is equipped with a chemical resistance and formed by a hard and almost rod state material. Further, by inserting the processing means 10 into the container 2 and performing either one of or both of a vertical movement in its long axial direction or rotational movement around the long axial center of the processing means 10, the sample 4 in the container 2 is processed.

3. 发明专利

JPH09327290A EXTRACTION AND PURIFICATION OF PLASMID DNA 失效
公开(公告)号：JPH09327290A
公开(公告)日：1997-12-22
申请号：JP14912796
申请日：1996-06-11
申请人： TOYO BOSEKI
发明人： ISHIDA YOSHIKAZU , IKEDA KATSUNORI , KAMIMURA HIDEKI , KAWAKAMI FUMIKIYO , KAWAMURA YOSHIHISA
IPC分类号： C12N15/09 , C07H1/08 , C07H21/04 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To purify the subject DNA in a high purity for a short time by adding a cytolytic liquid containing a chaotropic substance, an organic solvent and a nucleic acid-bonding solid-phase carrier to a microorganism or a cell containing a plasmid DNA, washing the resultant solid-phase carrier adsorbing the plasmid DNA thereon and then treating the washed solid-phase carrier with an eluent. SOLUTION: A cytolytic liquid at pH 3-6 containing a chaotropic substance comprising a guanidine thiocyanate and sodium acetate-hydrochloric acid, etc., an extracting liquid comprising an organic solvent, etc., such as a buffer solution-saturated phenol and a nucleic acid-bonding solid-phase carrier comprising particles, etc., containing a superparamagnetic metallic oxide are added, mixed or brought into contact with a microorganism or a cell holding a plasmid DNA to adsorb the plasmid DNA on the solid-phase carrier. The solid-phase carrier adsorbing the plasmid DNA thereon is then washed with a wash liquid and the plasmid DNA is eluted from the washed solid-phase carrier with an eluent to thereby extract and purify the plasmid DNA from the microorganism or cell in high purity for a short time without requiring complicated operations.

4. 发明专利

JP2000019171A ANALYSIS METHOD BY LABELED PROBE 失效
公开(公告)号：JP2000019171A
公开(公告)日：2000-01-21
申请号：JP18485298
申请日：1998-06-30
申请人： MATSUMOTO KAZUKO , TOYO BOSEKI
发明人： MATSUI KAZUHIRO , IKEDA KATSUNORI , TEJIMA SHINICHI , KAWAMURA YOSHIHISA , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To make an object substance measurable with high sensitivity by bonding a labeled body expressed by a specific formula, to a probe of nucleic acid or the like through a cross-linking group or the like so as to react with the object substance on an organismic sample, then adding a heavy metal to obtain a complex, and measuring the fluorescence of the complex. SOLUTION: The labeled body is expressed by formula I or formula II, in which (A1-A3 are each an aromatic group; R1-R3 are each : hydrogen atom or -COCH2COCnF2n+1; (n) is an integer of 1-6). This labeled body is bonded to a probe through a cross-linking group, or a cross-linking group and a linking group to obtain a labeled probe. The probe is selected from a group comprising nucleic acid, nucleic acid bonded protein, low molecular weight ligand and ligand receptor. The object substance is an analysis object component in an organismic sample, and nucleic acid, nucleic acid bonded protein, ligand or ligand receptor is preferable. The organismic sample is dipped in a buffer solution containing this labeled probe and incubated to react the labeled probe with the object substance, and further dipped in a buffer solution containing lanthanoid metal ion after washing, and the fluorescence of a produced fluorescent complex is measured.

5. 发明专利

JPH1038847A ELECTROPHORESIS METHOD AND SUPPORT BODY FOR ELECTROPHORESIS 失效
公开(公告)号：JPH1038847A
公开(公告)日：1998-02-13
申请号：JP19226196
申请日：1996-07-22
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , ISHIDA YOSHIKAZU , KAMIMURA HIDEKI
IPC分类号： G01N27/447
摘要： PROBLEM TO BE SOLVED: To make electrophoresis simply and speedily by diluting an electrophoresis support body adjusted at a concentration higher than during electrophoresis during electrophoresis and using the body. SOLUTION: As an electrophoresis support body, agar or agarose is highly refined, is heated and dissolved in a buffer solution component, is cooled, and gelled. The buffer solution component is composed of a component having a buffering capability, a pH formulation substance, and a nuclear acid dissolving and restricting component. Upon formulation of the support body, at least approx. 2% of agar or agarose is suspended in the buffer solution, the suspension solution is heated and dissolved at approx. 121 deg.C for approx. 10 minutes by using a high-pressure sterilization device, gel solution of its high concentration of approx. 2 to 5% is automatically formulated, and is gelled in a specified amount of volume, making it possible to be stored. This high-concentration gel block can be easily dissolved by adding water only during use and has its supperior operability and safety.

6. 发明专利

JPH0763767A LIQUID PROCESSING DEVICE FOR MEMBRANOUS CARRIER 失效
公开(公告)号：JPH0763767A
公开(公告)日：1995-03-10
申请号：JP21543093
申请日：1993-08-31
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , INOUE HIROAKI , OKA MASANORI
IPC分类号： G01N33/50 , G01N33/68
摘要： PURPOSE:To provide a device which can efficiently perform liquid processing against a membranous carrier by using a smaller amount of solution. CONSTITUTION:In a liquid processing device for membranous carriers, such a membranous carrier 6 as the membrane filter, etc., is put around the external surface of a roller 1. The roller 2 can be rotated by means of a shaft 2. A tank 3 is provided below the roller 1 with a small gap in between. The liquid holding section 3a of the tank 3 is formed in a nearly cylindrical shape. The carrier 6 comes into contact with a reactive solution 7 contained in the tank 3 as the roller 1 is rotated.

7. 发明专利

JPH0763763A METHOD AND DEVICE FOR BLOTTING 失效
公开(公告)号：JPH0763763A
公开(公告)日：1995-03-10
申请号：JP21542993
申请日：1993-08-31
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , INOUE HIROAKI , OKA MASANORI
IPC分类号： C12M1/26 , C12M1/34 , C12Q1/68 , G01N33/60
摘要： PURPOSE:To provide a method and device by which a nucleic acid or protein can be efficiently blotted even from such a carrier that has a large and thin transferring surface. CONSTITUTION:After placing a piece of nonwoven fabric 1 on a nonwoven fabric fixing table 2, a separating gel 5 holding the pattern of a nucleic acid or protein, membrane filter 6, water absorbing sheet 7, and plate 8 are successively put on the nonwoven fabric 1. A developing solution 3 is put in a groove 2b formed on the table 2 and the edge of the fabric 1 is dipped in the developing solution 3. The liquid 3 permeates through the fabric 1 and moves to the filter 6 from the gel 5. As the developing solution 3 moves, the nucleic acid or protein is blotted on the filter 6 from the gel 5.

8. 发明专利

JP2003149255A DISPENSING DEVICE MOVABLE TO RECTANGULAR COORDINATE AND CYCLINDERICAL COORDINATE 审中-公开
公开(公告)号：JP2003149255A
公开(公告)日：2003-05-21
申请号：JP2001350529
申请日：2001-11-15
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , NISHIYA YOSHIAKI
IPC分类号： B01J4/02 , B01L3/02 , G01N35/00 , G01N35/10
摘要： PROBLEM TO BE SOLVED: To expand the working range of the motor-operated pipette section of a dispensing device or the front end section of the pipette section which sucks and discharges a liquid. SOLUTION: The motor-operated pipette section which can suck and discharge the liquid can be exchanged automatically in accordance with dispending amounts. The liquid dispending device can arbitrarily expand its working area for sucking and discharging liquids by using a plurality of units, each of which is constituted by attaching a basic driving system to a rotatable driving mechanism and the rotatable driving mechanism to a driving mechanism that can move in the unmovable direction of the rotatable driving mechanism. In the basic driving system, the pipette section or the front end section of the pipette section which sucks and discharges the liquid is attached to a linearly driving mechanism that can move in one, two, or three of forward, backward, leftward, and rightward directions.

9. 发明专利

JP2003139739A SHAPE OF SAMPLE INJECTION PART FOR GEL FOR ELECTROPHORESIS 审中-公开
公开(公告)号：JP2003139739A
公开(公告)日：2003-05-14
申请号：JP2001335074
申请日：2001-10-31
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , NISHIYA YOSHIAKI
IPC分类号： G01N27/447 , C12M1/00
摘要： PROBLEM TO BE SOLVED: To provide a preparation method of a gel for electrophoresis used in analysis for DNA or protein. SOLUTION: A well preparing comb for the gel for the electrophoresis and for preparing the gel for the electrophoresis is characterized in that an area of a well opening part is wider than a bottom area of a well, in a shape of the well for injecting a sample of the gel for the electrophoresis. The sample gets easy to be injected into the well for the electrophoresis without deteriorating an analytical result to execute the analysis surely with reduced fatigue.

10. 发明专利

JP2001070827A DEVICE FOR PURIFYING NUCLEIC ACID AND PROTEIN 失效
公开(公告)号：JP2001070827A
公开(公告)日：2001-03-21
申请号：JP25182699
申请日：1999-09-06
申请人： TOYO BOSEKI
发明人： IKEDA KATSUNORI , SUZUKI MASAHIRO , KUSUMOTO MASAHIRO , NISHIYA YOSHIAKI , KAWAMURA YOSHIHISA
IPC分类号： C12N15/09 , B01D43/00 , B03C1/00 , C12N15/10 , C12Q1/68 , G01N1/34 , G01N35/00 , G01N35/02
摘要： PROBLEM TO BE SOLVED: To provide a purification device which is used for purifying microbes such as viruses and bacteria, nucleic acids such as DNA and RNA from the tissues of plants and animals or proteins such as an enzyme and an antibody and can carry out multiple-sample treatment and high speed treatment. SOLUTION: This purification device of nucleic acids and proteins by making good use of magnetic force and using attractable particles is provided with plural piston pumps, plural nozzles to/from which plural disposal chips can be automatically attached/detached and a mechanism for dividing/injecting a reagent to be used in the following step by a necessary amount or by a necessary number with high precision at high speed in parallel with a step to mix (agitate) a mixture of a sample and the reagent by using these pumps and nozzles. Thereby, the multiple-sample treatment and the high speed treatment are made possible by carrying out fully automatically the work from the mixing (agitating) of the attractable particles by making good use of magnetic force until the concentration and purification of the sample while discharging a small amount of waste.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式