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1. 发明专利

JP2002017354A NEW PURINE NUCLEOSIDE PHOSPHORYLASE 失效
公开(公告)号：JP2002017354A
公开(公告)日：2002-01-22
申请号：JP2000198927
申请日：2000-06-30
申请人： TOYO BOSEKI
发明人： HATTORI SHIZUO , KAWAMURA YOSHIHISA , YOSHIMOTO TADASHI , ITO KIYOSHI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12Q1/26 , C12Q1/48 , C12Q1/68 , C12R1/46
摘要： PROBLEM TO BE SOLVED: To provide a new purine nucleoside phosphorylase which has a low nucleosidase subactivity. SOLUTION: This new purine nucleoside phosphorylase which has a mixed nucleoside activity of

2. 发明专利

JP2757376B2 失效
公开(公告)号：JP2757376B2
公开(公告)日：1998-05-25
申请号：JP15619388
申请日：1988-06-24
申请人： TOYO BOSEKI KK
发明人： TSURU OONORI , YOSHIMOTO TADASHI
IPC分类号： C12N9/48 , C07K14/00 , C07K14/195 , C07K14/41 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/57 , C12R1/19 , C12R1/865

3. 发明专利

JPH0638769A DNA FRAGMENT HAVING GENETIC INFORMATION OF PROLINE IMINOPEPTIDASE AND ITS USE 失效
公开(公告)号：JPH0638769A
公开(公告)日：1994-02-15
申请号：JP7259892
申请日：1992-02-21
申请人： TOYO BOSEKI
发明人： TSURU ONORI , YOSHIMOTO TADASHI , KITAZONO ANA
IPC分类号： C12N9/48 , C12N15/09 , C12N15/57 , C12R1/19
摘要： PURPOSE:To provide a new DNA useful for producing proline iminopeptidase(PIP). CONSTITUTION:This invention is a DNA having PIP genetic information coding a base sequence of the formula. This DNA can be obtained from a Bacillus coagulance EK-19 (Bikoken stipulation No.12758), etc., as its donor or from a transformed microorganism as its donor to which PIP productivity is given by the gene recombination technique.

4. 发明专利

JPH119279A GENE FOR ENCODING GLYCEROL KINASE 失效
公开(公告)号：JPH119279A
公开(公告)日：1999-01-19
申请号：JP16726597
申请日：1997-06-24
申请人： TOYO BOSEKI
发明人： NISHIYA YOSHIAKI , KAWAMURA YOSHIHISA , YOSHIMOTO TADASHI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/00 , C12R1/01 , C12R1/19
摘要： PROBLEM TO BE SOLVED: To obtain the subject new gene useful for producing an enzyme having high stability suitable for measuring lipid and glycerol in blood and clinical examination, comprising a gene encoding a glycerol kinase having a specific amino acid sequence. SOLUTION: This gene comprises an amino acid sequence which is deficient in or substituted with one or plural amino acids or to which one or plural amino acids are added in a protein comprising an amino acid sequence of the formula or in the amino acid sequence of the formula, encodes a glycerol kinase which is a protein having glycerol kinase activity and is useful for producing a glycerol kinase suitable for measuring lipid and glycerol in blood, clinical examination medicine, enzymochemical study, etc. The gene is obtained by separating a chromosomal DNA from Thermus flavus TE3420 (DSM674) and subjecting the DNA to polymerase chain reaction(PCR) by using a partial DNA of glycerol kinase as a primer.

5. 发明专利

JPH05137582A DNA HAVING GENETIC INFORMATION ON PYROGLUTAMYL AMINOPEPTIDASE AND ITS USE 失效
公开(公告)号：JPH05137582A
公开(公告)日：1993-06-01
申请号：JP32816391
申请日：1991-11-15
申请人： TOYO BOSEKI
发明人： TSURU ONORI , YOSHIMOTO TADASHI
IPC分类号： C12N9/48 , C12N15/09 , C12N15/57
摘要： PURPOSE:To obtain the subject DNA, capable of coding an amino acid sequence of a polypeptide containing a pyroglutamyl aminopeptidase and producing the high-purity pyroglutamyl aminopeptidase useful as a reagent for research, etc., with high productivity. CONSTITUTION:Bacillus amyloliquefaciens IFO 14141 is cultured in a culture medium at 37 deg.C overnight by shaking culture and the bacterial cell is then collected by centrifugation and suspended in a 50mM tris(hydroxymethyl) aminomethane (Tris) hydrochloride buffer solution (pH 8.0). A lysozyme, a surfactant, etc., are added to carry out bacteriolysis. A solution of chloroform- phenol is then added to centrifuge the solution. An aqueous layer is collected and precipitated with ethanol to recover a DNA, which is then treated with a restriction enzyme, bound to a vector and transduced into Escherichia coli to perform transformation. The obtained transformant is cultured to select a clone capable of producing a pyroglutamyl aminopeptidase. The DNA is recovered from the clone and treated with a restriction enzyme to afford the objective DNA coding the amino acid sequence of a polypeptide containing the pyroglutamyl aminopeptidase.

6. 发明专利

JPH06303981A DNA HAVING GENETIC INFORMATION ON PROTEIN HAVING FORMALDEHYDE DEHYDROGENASE ACTIVITY AND PRODUCTION OF FORMALDEHYDE DEHYDROGENASE 失效
公开(公告)号：JPH06303981A
公开(公告)日：1994-11-01
申请号：JP9454793
申请日：1993-04-21
申请人： TOYO BOSEKI
发明人： ITO KIYOSHI , YOSHIMOTO TADASHI , TSURU ONORI , TAKAHASHI MARI
IPC分类号： C12N1/21 , C12N9/02 , C12N15/09 , C12N15/53 , C12R1/19 , C12R1/40
摘要： PURPOSE:To feed a large amount of a DNA having the non-glutathione- dependent formaldehyde dehydrogenase in a pure form at a low cost without producing other enzymes accompanying thereto in natural bacteria. CONSTITUTION:The DNA fragment contains a base sequence described in sequence No.1 of the sequence table and a recombinant vector has the DNA fragment. The transformant is obtained by transformation with the recombinant vector and the method for producing a formaldehyde dehydrogenase is characterized by culturing the transformant in a culture medium, producing the formaldehyde dehydrogenase and collecting the formaldehyde dehydrogenase. Although cleatininamide hydrolase, cleatininamidinohydrolase, sarcosine dehydrogenase, etc., are especially contained in the enzyme produced from natural bacteria and many steps are required for removing them, the steps for purification thereof can be simplified.

7. 发明专利

JPH10113173A NEW GLYCEROL KINASE AND PRODUCTION OF THE SAME 失效
公开(公告)号：JPH10113173A
公开(公告)日：1998-05-06
申请号：JP27245396
申请日：1996-10-15
申请人： TOYO BOSEKI
发明人： YOSHIMOTO TADASHI , NISHIYA YOSHIAKI , KAWAMURA YOSHIHISA
IPC分类号： C12N9/12 , C12Q1/48 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To obtain a new glycerol kinase suitable for quantitative measurement of lipid and glycerol in a sample and, having a stable glycerol kinse activity. SOLUTION: The glycerol kinase has the following physical and chemical properties and is an enzyme produced by Thermus flavus, excellent in thermal stability. Action: acts on gylcerol under the existence of ATP to produce glycerol-3-phosphate and ADP. Thermal stability: manifests >90% of activity after heating at 70 deg.C for 20min. Optimum temperature: about 65 deg.C (7.5pH) Molecular weight: about 58.000 (measured by an electrophoretic analysis; SDS-PAGE), about 220,000 (measured by gel filtration process). The quantitative measurement of lipid and glycelol can be carried out by using this glycelol kinase, especially, a high stability is manifested by using the glycerol kinase to a liquid reagents than using a conventional enzyme.

8. 发明专利

JPH07143881A DNA CONTAINING GENETIC INFORMATION OF PROTEIN HAVING CREATININE DEIMINASE ACTIVITY AND PRODUCTION OF CRETININE DEIMINASE 失效
公开(公告)号：JPH07143881A
公开(公告)日：1995-06-06
申请号：JP27548293
申请日：1993-11-04
申请人： TOYO BOSEKI
发明人： ITO KIYOSHI , YOSHIMOTO TADASHI , TSURU ONORI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/78 , C12R1/07 , C12R1/19
摘要： PURPOSE:To provide a process for producing a large amount of creatinine deiminase in pure form at a low cast by using a genetic engineering technique. CONSTITUTION:This invention relates to a DNA fragment having the genetic information of a creatinine deiminase which is a DNA coding the amino acid sequence described in the sequence table, a recombination vector containing the DNA fragment, a transformant transformed with the recombination vector and a process for the production of creatinine deiminase characterized by the culture of the transformant in a medium in the absence of creatinine and the separation of the produced creatinine deiminase from the cultured product.

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