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1. 发明专利

JP2005312445A Method for producing artificial polyclonal immunoglobulin 有权
标题翻译：生产人造多克隆免疫球蛋白的方法
公开(公告)号：JP2005312445A
公开(公告)日：2005-11-10
申请号：JP2005104770
申请日：2005-03-31
申请人： Sekisui Chem Co Ltd , Kazuo Suzuki , 積水化学工業株式会社 , 和男鈴木
发明人： SUZUKI KAZUO , FURUYA MASAHIRO , YAMAMOTO KENJI , ONO NAOHITO , TAKAHASHI HIROSHI , ARAYA YASUAKI , TOUGI AKIKO
IPC分类号： C12N15/09 , A61K39/395 , A61P31/04 , A61P37/02 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a polyclonal immunoglobulin according to a recombinant DNA technique adaptable to production of an immunoglobulin pharmaceutical preparation. SOLUTION: A plurality of species of genes encoding a polypeptide containing mutually different immunoglobulin variable regions are isolated. The resultant gene mixture is brought into contact with a vector and integrated to prepare a plurality of species of recombinant vectors corresponding to the various immunoglobulin variable regions and prepare a recombinant vector mixture. The resultant recombinant vector mixture is then brought into contact with a suitable host cell and transduced to prepare a plurality of species of recombinants corresponding to the various immunoglobulin variable regions and prepare a recombinant mixture. The obtained recombinant mixture is finally mixed and cultured to express a plurality of species of polypeptides corresponding to the various immunoglobulin variable regions in the recombinants. A polypeptide mixture thereof is prepared to produce the artificial polyclonal immunoglobulin. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供根据适用于产生免疫球蛋白药物制剂的重组DNA技术制备多克隆免疫球蛋白的方法。解决方案：分离编码含有相互不同的免疫球蛋白可变区的多肽的多种基因种类。使所得基因混合物与载体接触并整合以制备对应于各种免疫球蛋白可变区的多种重组载体，并制备重组载体混合物。然后将所得重组载体混合物与合适的宿主细胞接触并转导以制备对应于各种免疫球蛋白可变区的多种重组体，并制备重组混合物。将获得的重组混合物最终混合并培养以表达与重组体中各种免疫球蛋白可变区相对应的多种多肽。制备其多肽混合物以产生人造多克隆免疫球蛋白。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2012215535A Method for measuring matter, substrate for measurement of matter, and system for measuring matter 有权
标题翻译：测量方法，测量用基板和测量系统
公开(公告)号：JP2012215535A
公开(公告)日：2012-11-08
申请号：JP2011123364
申请日：2011-06-01
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： FURUYA MASAHIRO , AKAGI YOSHINORI , FUKUOKA MASATERU , NOZATO SEIJI
IPC分类号： G01N35/08 , G01N33/543 , G01N33/544 , G01N33/569 , G01N33/579 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a technique capable of performing measurement based on insoluble-aggregate production quickly and easily with high sensitivity.SOLUTION: A reaction liquid 25 containing an insoluble aggregate is brought into contact with an upstream side end 8 of a channel for movement of reaction liquid 6 provided in a substrate for measurement of matter 1. The reaction liquid 25 is introduced into the channel for movement of reaction liquid 6 by capillary force and moved in the channel for movement of reaction liquid 6. On the basis of the degree of the movement of the reaction liquid 25, the concentration of target matter contained in a sample is measured. Preferably, the generation of a gas is caused by an optical responsive generating component. As examples of insoluble-aggregate production reaction, Limulus reaction (LAL reaction) in endotoxin (lipopolysaccharide) measurement, latex agglutination reaction, and so on, are cited.
摘要翻译：要解决的问题：提供能够以高灵敏度快速且容易地基于不溶性聚集体生产进行测量的技术。解决方案：将含有不溶性聚集体的反应液25与设置在物质1的测定用基板中的反应液6的移动通道的上游侧端部8接触。将反应液25导入用于通过毛细管力移动反应液体6的通道，并在反应液体6的通道中移动。基于反应液体25的移动程度，测量样品中包含的目标物质的浓度。优选地，气体的产生由光学响应发电部件引起。作为不溶聚合物生成反应的实例，引用了内毒素（脂多糖）测定中的鲎反应（LAL反应），乳胶凝集反应等。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2011032276A Method of immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma, and method for producing monoclonal antibody 审中-公开
标题翻译：免疫动物的方法，用于免疫的组合物，用于生产抗体的方法，用于生产杂交瘤的方法和用于产生单克隆抗体的方法
公开(公告)号：JP2011032276A
公开(公告)日：2011-02-17
申请号：JP2010207530
申请日：2010-09-16
申请人： Jo Chiba , Sekisui Chem Co Ltd , 千葉丈 , 積水化学工業株式会社
发明人： CHIBA JO , HATA JUNICHI , NISHIGUCHI NAOKI , FURUYA MASAHIRO
IPC分类号： A61K48/00 , A61K39/00 , A61P37/02 , A61P43/00 , C12N5/10 , C12N15/02 , C12N15/09 , C12P21/08
CPC分类号： A61K39/00 , A61K39/0005 , A61K39/385 , A61K2039/53 , A61K2039/6068 , C07K16/2869 , C07K2319/35
摘要： PROBLEM TO BE SOLVED: To provide a method whereby a humoral immune response can be induced at a higher efficiency in producing an antibody against an antigen protein by gene immunization. SOLUTION: By administering a fused gene composed of a gene encoding an antigen protein as a whole or a part thereof attached to another gene encoding a chaperonin subunit or a chaperonin subunit ligation, the fused gene is expressed in the animal and thus a humoral immune response to the antigen protein is induced. An example of the chaperonin includes Escherichia coli GroEL. There is also provided a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种可以通过基因免疫在产生针对抗原蛋白的抗体中以更高的效率诱导体液免疫应答的方法。解决方案：通过施用由编码作为整体的抗原蛋白的基因或其附着于编码伴侣伴侣亚基的另一基因或伴侣伴侣亚基连接的融合基因，融合基因在动物中表达，因此，诱导针对抗原蛋白的体液免疫应答。伴侣蛋白的一个实例包括大肠杆菌GroEL。还提供了用于免疫的组合物，抗体的制备方法，杂交瘤的制造方法以及单克隆抗体的制造方法。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2010006745A Fused protein, fused protein-immobilized carrier, method for screening compound, screening composition and screening kit 审中-公开
标题翻译：融合蛋白，融合蛋白质固定载体，筛选化合物的方法，筛选组合物和筛选试剂盒
公开(公告)号：JP2010006745A
公开(公告)日：2010-01-14
申请号：JP2008168174
申请日：2008-06-27
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： SATO SHUCHI , IDENO AKIRA , FURUYA MASAHIRO
IPC分类号： C07K19/00 , C07K14/705 , C07K17/00 , C12N9/90 , C12N15/09 , G01N33/15 , G01N33/50 , G01N33/53
摘要： PROBLEM TO BE SOLVED: To provide a series of technology for readily screening compounds that specifically bind to endothelin receptors.
SOLUTION: A fused protein has ligand-binding activity and comprises an endothelin receptor and a protein having molecular chaperone activity or subunit(s) thereof which is bound to an N- or C-terminal of the endothelin receptor. Examples of the protein having the molecular chaperone activity are chaperonin and PPIase. A fused protein-immobilized carrier is obtained by immobilizing the fused protein onto a carrier. The fused protein or the fused protein-immobilized carrier is used in a screening method. A screening composition using the screening method and a screening kit are also provided.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一系列用于容易地筛选特异性结合内皮素受体的化合物的技术。解决方案：融合蛋白具有配体结合活性，并且包含内皮素受体和具有分子伴侣活性的蛋白质或其亚基结合到内皮素受体的N-或C-末端。具有分子伴侣活性的蛋白质的实例是伴侣伴侣和PPI酶。通过将融合的蛋白质固定在载体上获得融合蛋白质固定的载体。融合蛋白或融合蛋白固定化载体用于筛选方法。还提供了使用筛选方法和筛选试剂盒的筛选组合物。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2005013066A Fused protein, its production method and evaluation method using the same 有权
标题翻译：熔融蛋白质，其生产方法和使用该方法的评价方法
公开(公告)号：JP2005013066A
公开(公告)日：2005-01-20
申请号：JP2003181393
申请日：2003-06-25
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： FURUYA MASAHIRO , IDENO AKIRA
IPC分类号： C12N15/09 , C07K14/195 , C07K14/47 , C07K14/705 , C07K16/12 , C07K16/40 , C07K19/00 , C12N1/21 , C12N9/90 , C12P21/02 , C12Q1/02
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a fused protein comprising a secretion type protein and a target membrane protein, wherein the target membrane protein is bound to the C-terminal of the secretion type protein. SOLUTION: The method for preparing a recombinant membrane protein important in a research for developing a new medicine, and the recombinant membrane protein, by improving a recombinant protein expression system of bacterium. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供包含分泌型蛋白质和靶膜蛋白质的融合蛋白质，其中所述靶膜蛋白质与所述分泌型蛋白质的C-末端结合。解决方案：通过改进细菌的重组蛋白表达系统，制备重组膜蛋白重组蛋白在重组蛋白质表达系统中的应用研究新方法研究重组膜蛋白和重组膜蛋白。版权所有（C）2005，JPO＆NCIPI

6. 发明专利

JP2004041081A Method for screening fluorescent substance and method for producing fluorescent marker 审中-公开
公开(公告)号：JP2004041081A
公开(公告)日：2004-02-12
申请号：JP2002202999
申请日：2002-07-11
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： TOUGI AKIKO , FURUYA MASAHIRO
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for screening a fluorescent substance which is readily permeated through a membrane, is prepared as a fusion protein and has high sensitivity and to provide a method for producing the fluorescent marker. SOLUTION: The method for screening the fluorescent substance comprises (1) dividing a gene molecule group encoding a peptide to become a ligand in forming a chelate with a rare earth element ion by 1-several tens of gene molecules, (2) carrying out PCR in mutually separated containers to amplify the genes, (3) then dividing contents in each container, leaving a part of the divided contents, (4) carrying out a cell-free protein synthesis in the container storing the other part, adding the rare earth element ion to the container, assaying fluorescence of the contents in the container, selecting the contents in the container, having higher fluorescence intensity, (5) then selecting the contents in the container before the cell-free protein synthesis of the selected contents in the container from the divided contents of the process (3) and analyzing a gene base sequence and (6) repeating the processes (2)-(5) until the gene becomes one kind. COPYRIGHT: (C)2004,JPO

7. 发明专利

JP2004026714A Sustained-release preparation 审中-公开
公开(公告)号：JP2004026714A
公开(公告)日：2004-01-29
申请号：JP2002184789
申请日：2002-06-25
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： IZUMOTO YOSHITAKA , FURUYA MASAHIRO , HATA JUNICHI
IPC分类号： A61K47/48 , A61K38/00 , A61P7/02 , A61P25/18 , A61P29/00
摘要： PROBLEM TO BE SOLVED: To provide a sustained-release preparation in which carrier proteins of both almost all physiologically active proteins and low-molecular medicines are simply prepared, the physiologically active proteins or the low-molecular medicines are provided with sustained release properties and their pharmacodynamic effects are stably and efficiently developed.
SOLUTION: This sustained-release preparation comprises a fusion protein in which a chaperonin and the carrier protein combined with a physiologically active protein or a low-molecular medicine are linked through a peptide bond. The carrier protein combined with the physiologically active protein or the low-molecular medicine is stored in the inside of the chaperonin.
COPYRIGHT: (C)2004,JPO

8. 发明专利

JP2014161252A Recombinant cells 审中-公开
标题翻译：重组细胞
公开(公告)号：JP2014161252A
公开(公告)日：2014-09-08
申请号：JP2013033532
申请日：2013-02-22
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： FURUYA MASAHIRO , UENISHI SHOTA , IWASA KOICHIRO
IPC分类号： C12N1/21 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide recombinant cells that can produce crotonyl-CoA from synthetic gas etc.SOLUTION: Provided is a recombinant cell that can produce crotonyl-CoA from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid and methanol, which recombinant cell is prepared by introducing a gene encoding at least one enzyme selected from the group consisting of acetoacetyl-CoA thiolase, 3-hydroxybutyryl-CoA dehydrogenase and 3-hydroxybutyrate-CoA dehydratase to a host cell having functions to synthesize acetyl-CoA from methyltetrahydrofolate, carbon monoxide and CoA to be expressed in the host cell.
摘要翻译：要解决的问题：提供可以从合成气体等产生巴豆酰辅酶A的重组细胞。解决方案：提供一种可从至少一种选自一氧化碳，二氧化碳的C1化合物生产巴豆酰辅酶A的重组细胞，甲酸和甲醇，通过将编码选自乙酰乙酰辅酶A硫解酶，3-羟基丁酰辅酶A脱氢酶和3-羟基丁酸酯-CoA脱水酶中的至少一种酶的基因导入具有功能的宿主细胞来制备重组细胞从甲基四氢叶酸合成乙酰辅酶A，在宿主细胞中表达一氧化碳和CoA。

9. 发明专利

JP2010159218A Fused protein, protein complex, and method for analyzing structure of objective protein 审中-公开
标题翻译：融合蛋白，蛋白复合物和分析目标蛋白结构的方法
公开(公告)号：JP2010159218A
公开(公告)日：2010-07-22
申请号：JP2009001495
申请日：2009-01-07
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： IDENO AKIRA , SATO SHUCHI , FURUYA MASAHIRO
IPC分类号： C07K19/00 , C07K14/195 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a series of techniques which more ensure and facilitate the structural analysis of an objective protein crystallized.
SOLUTION: There is provided the fused protein in which a chaperonin connection product prepared by connecting two or more chaperonin subunits in series is connected to an objective protein and which can form a ring structure in which the chaperonin subunits are arranged in a ring-like shape, wherein the chaperonin connection product comprises a plurality of kinds of chaperonin subunits. The connection order of the chaperonin subunits in the chaperonin connection product is preferably an order giving a non-point symmetry, when the adjacent chaperonin subunits are arranged on an identical circumference in an equal distance. The fused protein facilitates to equalize the orientation of the objective proteins on crystallization, thereby enabling to surely obtain the X-ray diffraction image of the objective protein.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一系列更确保和促进结晶的目标蛋白质的结构分析的技术。提供了融合蛋白，其中通过将两个或更多个伴侣蛋白亚基串联连接而制备的伴侣伴侣连接产物连接到目的蛋白，并且可以形成其中伴侣蛋白亚基排列成环的环结构其中所述伴侣伴侣连接产物包含多种伴侣伴侣亚单位。伴侣蛋白亚基在伴侣蛋白连接产物中的连接顺序优选是当相邻的伴侣蛋白亚基以等距离排列在相同圆周上时给出非点对称的顺序。融合蛋白有助于平衡目标蛋白在结晶上的取向，从而能够可靠地获得目的蛋白的X射线衍射图像。版权所有（C）2010，JPO＆INPIT

10. 发明专利

JP2006280239A Activated inclusion body and its production method, isolated gene, expression vector, and method for producing target protein 审中-公开
标题翻译：活性包涵体及其生产方法，分离的基因，表达载体和产生目标蛋白的方法
公开(公告)号：JP2006280239A
公开(公告)日：2006-10-19
申请号：JP2005102455
申请日：2005-03-31
申请人： Sekisui Chem Co Ltd , 積水化学工業株式会社
发明人： NISHIGUCHI NAOKI , IDENO AKIRA , FURUYA MASAHIRO
IPC分类号： C12N15/09 , C07K14/195 , C07K14/435 , C07K19/00 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To facilitate the purification of a target protein expressed by a recombinant DNA technique. SOLUTION: This activated inclusion body comprising a fusion protein of a protecting polypeptide and a target protein, and having the activity of the target protein. The activated inclusion body holds the target protein in a correctly folded normal protein state. The correctly folded target protein can be cut out from the fused protein without solubilizing the activated inclusion body. The protecting polypeptide is a polypeptide having a mol.wt. of about 17,000 and comprising the N-terminal side portion of a long type FKBP type PPIase derived from an archaebacterium. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：促进由重组DNA技术表达的靶蛋白的纯化。解决方案：这种活化的包含身体的包含保护性多肽和靶蛋白的融合蛋白，并具有靶蛋白的活性。活化的包涵体将目标蛋白质保持正确折叠的正常蛋白质状态。正确折叠的目标蛋白质可以从融合的蛋白质切出而不增溶活化的包涵体。保护性多肽是具有mol.wt. 约17,000并且包含源自古细菌的长型FKBP型PPI酶的N-末端侧部分。版权所有（C）2007，JPO＆INPIT

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