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1. 发明专利

JPH07188292A GLYCOPROTEIN DERIVED FROM HUMAN, PHYSIOLOGICALLY ACTIVE FACTOR COMPOSED OF THE SAME GLYCOPROTEIN AND PHARMACEUTICAL PREPARATION CONTAINING THE SAME ACTIVE INGREDIENT 失效
公开(公告)号：JPH07188292A
公开(公告)日：1995-07-25
申请号：JP21818394
申请日：1994-08-19
申请人： SNOW BRAND MILK PROD CO LTD
发明人： TOO KANJI , MITSUDA SHINJIRO , SHIMA NOBUYUKI , ITAGAKI KOJI , NAGAO MASAYA
IPC分类号： C12N15/09 , A61K38/00 , A61P1/16 , A61P35/00 , A61P35/02 , A61P37/04 , A61P43/00 , C07K14/47 , C07K14/76 , C12N5/10 , C12P21/02 , C12R1/91
摘要： PURPOSE:To obtain a new glycoprotein, available from a culture supernatant of a fibroblast derived from a human and useful as an antltumor agent, a cell immunopotentiator, etc. CONSTITUTION:This glycoprotein is obtained from a culture supernatant of a fibroblast derived from a human and has the following properties: molecular weight: 78000+ or -2000 or 74000(+)2000 in a nonreduced state and a common band (A) of 52000(+)2000 and two bands of a band (B) of 30000+ or -2000 and a band (C) of 26000+ or -2000 in a reduced state (measured by the SDS-polyacrylamide electrophoresis); isoelectric point: 7.4-8.6; thermal stability:stable even by heating at 60 deg.C for 10min; pH stability: stable within the range of pH6-9; saccharide chain: manifesting the adsorptivity for concanavalin A-Sepharosce(R); physiological activity: capable of suppressing the proliferation of a KB cell, an HeLa cell and an L-929 cell without suppressing the proliferation of an IMR-90 cell; reactivity to antibody: without neutralizing the disorder activity by an anti-TNF antibody, an antilymphotoxin antibody and an antiinterferon beta antibody and amino acid sequence at the N-terminal; formula I or II (X is unidentified). Furthermore, the protein is obtained by a genetic engineering technique.

2. 发明专利

JPS62236480A METHOD FOR CULTURING ADHESIVE ANIMAL CELL AND APPARATUS THEREFOR 失效
公开(公告)号：JPS62236480A
公开(公告)日：1987-10-16
申请号：JP7788786
申请日：1986-04-04
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MITSUDA SHINJIRO , MATSUDA YOSHIAKI , KUMAZAWA EITARO
IPC分类号： C12M3/00 , C12N5/02 , C12N11/14
摘要： PURPOSE:To obtain the titled practical culture apparatus, by uniformizing size of ceramic particles having specific void ratio and adhesion surface area/medium volume ratio and composed mainly of alumina, using the particles as a filler of a column and placing an exchange material in the middle of the filler. CONSTITUTION:The carrier 4 to be filled in a column 1 is a granular ceramic having uniformized particle size of 5-9 mesh and composed mainly of alumina having a void ratio of 80-90%, an S/V value of 100-150 (S is adhesion surface area of cell adhering to the surface of the carrier; V is volume of medium) and excellent cell adhesivity. A porous exchange material 5 having medium- discharging function and supplying function is placed in the middle of the column 1.

3. 发明专利

JPH0387172A CELL-ENCLOSING CAPSULE AND ITS PREPARATION 失效
公开(公告)号：JPH0387172A
公开(公告)日：1991-04-11
申请号：JP22421589
申请日：1989-08-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MANO TAKASHI , MITSUDA SHINJIRO , KUMAZAWA EITARO , IIJIMA SHINJI , KOBAYASHI TAKESHI
IPC分类号： C12N11/08 , B01J13/04 , C12N5/00 , C12N5/10
摘要： PURPOSE:To prepare the subject capsule having excellent durability and suitable for producing a substance by a cell culture method by immobilizing cells with a gelling agent and subsequently encapsulating the gelled product in an urethane polymer coating layer having a specific structure. CONSTITUTION:A cell-containing gel prepared by immobility plant or animal cells with a gelling agent such as polysaccharide or gelatin is immersed in an urethane prepolymer (preferably a hydrophilic NCO-terminated prepolymer prepared from toluene diisocyanate and polyoxyethylenepolypropylenepolyol) containing 0.01-1% of water-containing fine particles (e.g. gelatin or starch) to polymerize the gel with the prepolymer, thereby coating the gelled product with the urethane polymer layer having many pores formed therein to provide the objective capsule.

4. 发明专利

JPS6121052B2 JPS6121052B2 - 失效
公开(公告)号：JPS6121052B2
公开(公告)日：1986-05-24
申请号：JP6366482
申请日：1982-04-16
申请人： Snow Brand Milk Prod Co Ltd
发明人： MITSUDA SHINJIRO , YADONOBE YUKITAKA , UEDA KUNIO , KUROSAWA SEIJI
IPC分类号： A23C19/09 , A23C19/032 , A23C19/06

5. 发明专利

JPH0925297A HUMAN-DERIVED GLYCOPROTEIN AND PHYSIOLOGICALLY ACTIVE FACTOR CONSISTING OF THE SAME AND PHARMACEUTICAL PREPARATION WITH THE SAME AS ACTIVE INGREDIENT 失效
公开(公告)号：JPH0925297A
公开(公告)日：1997-01-28
申请号：JP19145896
申请日：1996-07-01
申请人： SNOW BRAND MILK PROD CO LTD
发明人： TOO KANJI , MITSUDA SHINJIRO , SHIMA NOBUYUKI , ITAGAKI KOJI , NAGAO MASAYA
IPC分类号： C12N15/09 , A61K38/00 , A61P1/16 , A61P9/14 , A61P35/00 , A61P35/02 , A61P37/00 , A61P43/00 , C07K1/22 , C07K14/52 , C12P21/02 , C12R1/91
摘要： PROBLEM TO BE SOLVED: To provide a new glycoprotein obtained from the supernatant resulted from culturing human-derived fibroblast, having a specific N-terminated amino acid sequence, presenting antineoplastic activity and other physiological activities, having a sugar chain linkable to ConA, thus useful as e.g. an antineoplastic agent. SOLUTION: This new glycoprotein is obtained from the supernatant resulted from culturing human-derived fibroblast, having antineoplastic activity and other physiological activities, and also having the following characteristics: molecular weight (determined by SDS electrophoresis): 78,000±2,000 or 74,000±2,000 when non-reduced; when reduced, presenting a common band A (molecular weight: 52,000±2,000) and two bands i.e., band B (30,000±2,000) and band C (26,000±2,000); isoelectric point: 7.4-8.6; thermal stability: stable under heating at 60 deg.C for 10min; pH stability: stable at pH6-9; the sugar chain presenting adsorbability to concanavalin A (ConA) cephalose; and suppressing the proliferation of KB cells, HeLa cells and L-929 cells; the subchains B and C each having an N-terminated amino acid sequence of formula I or II (X is unidentified).

6. 发明专利

JPH0768272B1 失效
公开(公告)号：JPH0768272B1
公开(公告)日：1995-07-26
申请号：JP50427190
申请日：1990-03-09
申请人： SNOW BRAND MILK PROD CO LTD
发明人： HIGASHIO KANJI , MITSUDA SHINJIRO , SHIMA NOBUYUKI , ITAGAKI YASUHARU , NAGAO MASAYA
IPC分类号： C12N15/00 , A61K38/00 , C07K14/475 , C07K14/52 , C12N15/09 , C12N15/19 , C12P21/02

7. 发明专利

JPH0499483A METHOD FOR PERFUSION CULTURE OF ANIMAL CELL 失效
公开(公告)号：JPH0499483A
公开(公告)日：1992-03-31
申请号：JP21521390
申请日：1990-08-14
申请人： SNOW BRAND MILK PROD CO LTD
发明人： KAWAHARA HIROYUKI , MITSUDA SHINJIRO , KUMAZAWA EITARO
IPC分类号： C12M3/06 , C12N5/07 , C12N5/071
摘要： PURPOSE:To culture animal cells in a high density for a long period by taking out a suspensible animal cell-suspending culture solution, separating the cells from the culture solution with a flat plate filtering membrane intermittently washed and having a specific pore diameter and subsequently returning the Separated cells together with a fresh medium to a culture tank for the continuous exchange of the medium. CONSTITUTION:In a method for perfusion-culturing animal cells by charging a medium in a stirring culture tank BR, adding the animal cells in the medium, aerating the medium with air containing 5% of CO2, simultaneously stirring the culture tank slowly for culturing the cells, taking out a part of the culture solution suspending the suspensible animal cells, separating the animal cells from the culture solution by a filtering system equipped with a flat plate filter membrane having pores of 0.2-0.8mu and subsequently returning the animal cells together with a fresh medium to the culture tank BR for the continuous exchange of the medium, the flat plate filter membrane is intermittently washed with the medium in the same direction as the circulation direction of the culture solution, thereby permitting to subject the suspensible animal cells to a continuous perfusion culture for a long period.

8. 发明专利

JPH0386231A PRODUCTION OF GELLED CAPSULE COATED WITH COATING AGENT 失效
公开(公告)号：JPH0386231A
公开(公告)日：1991-04-11
申请号：JP22421689
申请日：1989-08-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MANO TAKASHI , MITSUDA SHINJIRO , KUMAZAWA EITARO , IIJIMA SHINJI , KOBAYASHI TAKESHI
IPC分类号： C12N11/08 , B01J13/04 , C12N5/00 , C12N5/10
摘要： PURPOSE:To continuously coat a gelled capsule with a coating agent soln. by previously incorporating inert and high-sp.gr. fine powder in the gelled capsule and adjusting the descending velocity of the capsule in a duplex nozzle. CONSTITUTION:The duplex nozzle 9 consisting of the inner pipe 8 for the descending gelled capsule and the outer pipe 7 for the descending coating agent is used, and the capsule is coated at the tip of the nozzle with a soln. of the coating agent (e.g. urethane prepolymer). In this case, inert and high-sp.gr. fine powder (e.g. alumina fine powder) is previously incorporated into the capsule to adjust the descending velocity of the capsule. As a result, the capsule is continuously and efficiently coated with the coating agent.

9. 发明专利

JPH02117388A CELL CULTURE METHOD WITH ADSORPTION COLUMN CONNECTED AND SYSTEM THEREFOR 失效
公开(公告)号：JPH02117388A
公开(公告)日：1990-05-01
申请号：JP27123888
申请日：1988-10-27
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MITSUDA SHINJIRO , HIRABAYASHI NORIHISA , MATSUDA YOSHIAKI , KUMAZAWA EITARO
IPC分类号： C12P1/00 , C12M3/00 , C12N5/07 , C12N5/071 , C12N5/10 , C12N9/64 , C12R1/91
摘要： PURPOSE:To continuously, advantageously produce esp. physiologically active substances by introducing a culture solution of animal cells, through a culture medium circulating tank, into an adsorption column with selective adsorptivity for the target substances and by circulating only the culture solution, through said tank, to a culture tank. CONSTITUTION:When useful substances are to be produced by culture of animal cells, first using a culture medium for cell growth, generally a serum-contg. medium the cells are grown in a culture tank 1 to a density of 10 -10 per ml of culture solution. Thence, the culture tank 1 and the whole system are cleaned using e.g., a serum-free medium. Valves are then put to changeover and an adsorption column 2 and a line 6 are connected, and the culture medium is defined as production medium, and the culture solution is circulated between the column 2 and the culture tank 1 through a medium-circulating tank 3. Thereby, production of useful substances, using the cells, can be made continuously and advantageously.

10. 发明专利

JPS62118878A CELL CULTURE UNIT 失效
公开(公告)号：JPS62118878A
公开(公告)日：1987-05-30
申请号：JP25826985
申请日：1985-11-18
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MATSUDA YOSHIAKI , MITSUDA SHINJIRO
IPC分类号： C12M3/04 , C12M3/00
摘要： PURPOSE:Cultivation dishes of a specific shape is set in a culture tank in multiple stages with a certain interval and each dish is equipped with a sintered metal filter at its center to supply the culture medium therefrom whereby mass cultivation of adhesive cells is possible. CONSTITUTION:Cutivation dishes 3 which have the bottom 3a inclining toward the periphery and the cross section of reversed V-shape at the periphery, are set in multistages in the tank 1. Individual dishes 3 is equipped with a sintered metal filter 4 at the center to feed the culture medium therefrom. Further, an overflow outlet 8 is formed at the bottom of the tank. In other words, the medium is fed from inlet 2a, passed through filters into each dish. The medium whose nutrients have been consumed by proliferation of the cells is allowed to overflow from the periphery of the dishes for recovery. The recovered medi um can be utilized again, by mixing with waste or the make-up medium. Thus, mass cultivation of adhesive cells becomes possible.

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