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    • 2. 发明专利
    • Fluorescent method for analyzing molecule using evanescent illumination
    • 用分析方法分析荧光的荧光方法
    • JP2003294631A
    • 2003-10-15
    • JP2002097879
    • 2002-03-29
    • Olympus Optical Co Ltdオリンパス光学工業株式会社
    • KOBAYASHI SAYOKOKATO NORIKO
    • G01N21/64G01N33/483
    • PROBLEM TO BE SOLVED: To provide a method and an apparatus for analyzing a behavior and a state of molecules adjacent to a cell membrane. SOLUTION: The method that analyzes a state of a substance to be targeted, is provided with a first step in which a fluorescent labeled substance is added to the substance to be targeted in a sampled specimen, a second step in which fluorescent signals generated in the sampled specimen are continuously detected at a plurality of moments while carrying out an evanescent illumination for the sampled specimen simultaneously with or after adding the labeled substance in the first step, and a third step in which the amount of fluctuation per one molecule of the substance to be targeted is analyzed based on the fluorescent signals obtained by observing in the second step, thereby analyzing the state of the substance to be targeted. COPYRIGHT: (C)2004,JPO
    • 待解决的问题:提供用于分析与细胞膜相邻的分子的行为和状态的方法和装置。 解决方案:分析待靶向物质的状态的方法具有第一步骤,其中将荧光标记物质添加到待采样样品中的待靶物质,第二步骤,其中荧光信号 在第一步骤中,在添加标记物质之后或之后同时对取样样本进行ev逝照明,在多个时刻连续地检测取样样本中产生的第三步骤,其中每分子 基于通过在第二步骤中观察获得的荧光信号分析待靶向物质,从而分析待靶向物质的状态。 版权所有(C)2004,JPO