专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2007259775A Method for efficiently producing transgenic silkworm 有权
标题翻译：有效生产转基因丝绸的方法
公开(公告)号：JP2007259775A
公开(公告)日：2007-10-11
申请号：JP2006090174
申请日：2006-03-29
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： TAMURA TOSHIKI , SETSUTSU HIDEKI , KOBAYASHI ISAO , KANDA TOSHIO , KOJIMA KATSURA , UCHINO KEIRO
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a transgenic silkworm in high efficiency. SOLUTION: The production efficiency of a transgenic silkworm is remarkably improved by introducing a transposon containing arbitrary gene inserted therein and mRNA of its transferase injected into the egg of silkworm and producing a silkworm from the egg. Concretely, an mRNA synthesized in vitro is used as a supplying source of a transferase of transposon piggyBac in place of conventional helper plasmid to remarkably improve the production efficiency of transgenic silkworm. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供高效率生产转基因蚕的方法。解决方案：通过引入含有插入其中的任意基因的转座子和转移酶的mRNA被注入到蚕卵中并从鸡蛋中产生蚕，转基因蚕的生产效率显着提高。具体地，将体外合成的mRNA用作转座子piggyBac的转移酶的供应源代替常规的辅助质粒，以显着提高转基因蚕的生产效率。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2012105598A Method for producing transformed silkworm 审中-公开
标题翻译：生产改性丝绸的方法
公开(公告)号：JP2012105598A
公开(公告)日：2012-06-07
申请号：JP2010257764
申请日：2010-11-18
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： TATEMATSU KENICHIRO , SEZUTSU HIDEKI , UCHINO KEIRO , KOBAYASHI ISAO
IPC分类号： A01K67/033
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently producing transformed silkworms.SOLUTION: Blasticidin of a prescribed concentration is orally taken by silkworms to which a blasticidin-resistant gene is introduced as a selection marker gene, so that transformed silkworms can be efficiently selected based on the death or survival of the silkworms. Particularly, the efficiency in selecting transformed silkworms can be significantly improved when the blasticidin-resistant gene is introduced into silkworms in such a way as to be expressed as a non-secretor protein and at least 10 μg of blasticidin relative to 1 g of feed is orally taken by first-instar larvae.
摘要翻译：待解决的问题：提供一种有效生产转化的蚕的方法。解决方案：通过引入杀稻瘟素抗性基因的蚕口服摄取规定浓度的杀稻瘟素作为选择标记基因，从而可以基于蚕的死亡或存活有效地选择转化的蚕。特别地，当将杀稻瘟素抗性基因以表达为非分泌蛋白的方式引入蚕中时，可以显着改善选择转化的蚕的效率，并且相对于1g饲料为至少10μg的杀稻瘟素由一龄幼虫口服。版权所有（C）2012，JPO＆INPIT

3. 发明专利

JP2009254324A β-N-ACETYLHEXOSAMINIDASE 有权
公开(公告)号：JP2009254324A
公开(公告)日：2009-11-05
申请号：JP2008110297
申请日：2008-04-21
申请人： Katakura Industries Co Ltd , National Institute Of Agrobiological Sciences , Osaka Univ , 国立大学法人大阪大学 , 片倉工業株式会社 , 独立行政法人農業生物資源研究所
发明人： NOMURA TAKESHI , ISHIYAMA SEIJI , IKEDA SHINKO , USAMI AKIHIRO , FUJIYAMA KAZUHITO , SEKI TATSUJI , OKADA TAKAHIRO , TAMURA TOSHIKI , KOBAYASHI ISAO , SEZUTSU HIDEKI , MITA KAZUHIDE
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/36
摘要： PROBLEM TO BE SOLVED: To provide a DNA encoding a protein having β-N-acetylhexosaminidase activity.
SOLUTION: Provided are a silkworm protein having β-N-acetylhexosaminidase activity, a DNA encoding the protein, a recombinant vector containing the DNA, a transformant containing the recombinant vector, and a method for producing β-N-acetylhexosaminidase.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供编码具有β-N-乙酰基己糖胺酶活性的蛋白质的DNA。解决方案：提供具有β-N-乙酰基己糖胺酶活性的蚕蛋白质，编码该蛋白质的DNA，含有该DNA的重组载体，含有该重组载体的转化体，以及一种生产β-N-乙酰氨基己糖苷酶的方法。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2007028966A Method for producing foreign enzyme protein, transgenic silkworm used therefor and method for producing the same 有权
标题翻译：用于生产外源酶蛋白的方法，用于其的转基因丝及其生产方法
公开(公告)号：JP2007028966A
公开(公告)日：2007-02-08
申请号：JP2005215340
申请日：2005-07-26
申请人： National Institute Of Agrobiological Sciences , Nitto Boseki Co Ltd , 日東紡績株式会社 , 独立行政法人農業生物資源研究所
发明人： KATAYAMA KATSUHIRO , OHASHI KENYA , KIYOKAWA IWAO , TAMURA TOSHIKI , KOBAYASHI ISAO , UCHINO KEIRO
IPC分类号： C12N9/12 , C07K14/435 , C07K19/00 , C12N9/08 , C12N9/16 , C12N15/09
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a new method for production with which a foreign enzyme protein is produced by a transgenic silkworm. SOLUTION: The high-molecular weight foreign enzyme protein can easily be obtained in a large amount from a silk gland or a silk thread of the transgenic silkworm as follows. The transgenic silkworm is prepared by transferring a gene group containing a first gene containing a gene of GAL4 or an analog thereof linked to the downstream side of a promoter for inducing the expression specifically in the silk gland and a second gene containing a gene of a protein expressed specifically in the silk gland and linked to the downstream side of a gene of a UAS (an upstream activating sequence) and a gene of the foreign enzyme protein linked to the downstream side of the gene of the protein expressed specifically in the silk gland into the chromosome. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种通过转基因蚕产生外源酶蛋白的新的生产方法。解决方案：高分子量外源酶蛋白可以容易地从转基因蚕的丝腺或丝线中大量获得，如下。通过转移含有含有GAL4基因的第一基因的基因组或其连接于启动子下游侧的类似物的基因组来制备转基因蚕丝，以在丝腺中特异性诱导表达，以及含有蛋白质基因的第二基因在丝腺中特异性表达并连接到UAS（上游激活序列）的基因的下游侧和与丝腺特异性表达的蛋白质的基因的下游侧连接的外源酶蛋白的基因成为染色体。版权所有（C）2007，JPO＆INPIT

5. 发明专利

JP2007202554A Bombyx mori densovirus type 2-sensitive gene and resistant gene and utilization thereof 有权
标题翻译： BOMBYX MORI DENSOVIRUS型2敏感基因和抗性基因及其利用
公开(公告)号：JP2007202554A
公开(公告)日：2007-08-16
申请号：JP2006350801
申请日：2006-12-27
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： MONNO KEIKO , MITA KAZUHIDE , ITO KATSUHIKO , TAMURA TOSHIKI , KOBAYASHI ISAO
IPC分类号： C12N15/09 , A01K67/04 , A61K31/7088 , A61K38/00 , A61K39/395 , A61K48/00 , A61P31/12 , C07K14/435 , C07K16/18 , C12N5/10 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/50 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide Bombyx mori Densovirus type 2-sensitive gene and nsd-2 (a resistant gene), to provide transgenic Bombyx mori utilizing the gene, to provide an imparting agent for sensitivity to Bombyx mori Densovirus type 2 virus or an inhibitor of the Bombyx mori Densovirus type 2 virus infection, to provide a method for judging the sensitivity to the Bombyx mori Densovirus type 2 virus and to provide a reagent for judgment. SOLUTION: A linkage analysis of a population selected with a chromosome walking and virus by forming BAC contig utilizing Bombyx mori genome information is carried out to find out deletion specific for resistant lineage and identify the gene. The deletion in the resistant species is reflected even in a transcript. Thereby, it is made clear that a product shorter than sensitive lineage by about 1 kb is transcribed. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：为了提供蚕蛾Densovirus 2型敏感基因和nsd-2（一种抗性基因），利用该基因提供转基因蚕蛾，为Bombyx mori Densovirus type 2提供一种赋予敏感性的赋予剂病毒或Bombyx mori Densovirus 2型病毒感染的抑制剂，提供一种判断对家蚕Densovirus 2型病毒敏感性的方法，提供用于判断的试剂。解决方案：通过利用蚕蛾基因组信息形成BAC contig进行染色体行走和病毒选择的群体的连锁分析，以找出抗性谱系的特异性缺失并鉴定该基因。抗性物种的缺失也反映在誊本中。因此，显而易见，转录了比敏感谱系短约1kb的产物。版权所有（C）2007，JPO＆INPIT

6. 发明专利

JP2013230155A Transgenic silkworm producing antibody and method for producing the same 有权
标题翻译：生产抗体的转基因丝和其生产方法
公开(公告)号：JP2013230155A
公开(公告)日：2013-11-14
申请号：JP2013129087
申请日：2013-06-20
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所 , Nitto Boseki Co Ltd , 日東紡績株式会社
发明人： TAMURA TOSHIKI , KOBAYASHI ISAO , KANDA TOSHIO , UCHINO KEIRO , KATAYAMA KATSUHIRO , OHASHI KENYA , KIYOKAWA IWAO , ARAI HISAE , FUNABASHI NORIYUKI
IPC分类号： C12N15/09 , A01K67/033 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/08
CPC分类号： C07K16/00 , A01K67/0335 , A01K67/04 , A01K2217/05 , A01K2227/703 , A01K2267/01 , C07K14/43586 , C07K2317/10 , C07K2317/20 , C07K2319/02 , C12N15/8509 , C12N2830/002 , C12N2830/003
摘要： PROBLEM TO BE SOLVED: To provide a method for mass-producing a recombinant antibody close to that made by mammals, using a recombinant silkworm.SOLUTION: A promoter of DNA encoding a protein expressing in a silkgland-specific way is provided. A transgenic silkworm having DNA encoding a recombinant antibody subject to direct or indirect expression control by the promoter is also provided. The transgenic silkworm secreting the recombinant antibody in the silkgland is prepared. It has been confirmed that the recombinant antibody produced from the transgenic silkworm's silkgland has activity.
摘要翻译：要解决的问题：提供一种使用重组蚕来接近由哺乳动物制造的重组抗体进行大规模生产的方法。解决方案：提供编码以silkgland特异性方式表达的蛋白质的DNA的启动子。还提供了一种具有编码重组抗体的DNA的转基因蚕，该重组抗体经由启动子的直接或间接表达控制。制备在silkgland中分泌重组抗体的转基因蚕。已经证实，从转基因蚕的silkgland产生的重组抗体具有活性。

7. 发明专利

JP2012187123A Method for producing proteins utilizing silkworm middle silk gland-specific gene expression system 审中-公开
标题翻译：生产使用丝光中丝蛋白特异性基因表达系统的蛋白质的方法
公开(公告)号：JP2012187123A
公开(公告)日：2012-10-04
申请号：JP2012146507
申请日：2012-06-29
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： TAMURA TOSHIKI , SEZUTSU HIDEKI , KOBAYASHI ISAO , KOJIMA KATSURA , KANDA TOSHIO , UCHINO KEIRO
IPC分类号： A01K67/027 , C07K1/00 , C12N15/09 , C12P21/00
摘要： PROBLEM TO BE SOLVED: To provide a method for producing recombinant proteins in the silkworm middle silk gland.SOLUTION: Transgenic silkworms having GFP whose expression is controlled by a sericin gene promotor are produced. Observation of the silk glands of the larva in the last instar of the silkworm shows fluorescence only in the middle silk glands. It is also found that GFP is secreted from the middle silk gland cells from around the spinning stage, and that GFP is moved into the gland lumen. Finally, GFP is spun into cocoon filaments, and a cocoon containing a large amount of GFP is produced. In this way, it is discovered that recombined proteins can be produced in the middle silk glands by utilizing the promotor region of the sericin gene, and also discovered that the recombinant proteins produced in the middle silk gland can be easily secreted into the inner lumen of the middle silk glands.
摘要翻译：待解决的问题：提供一种在蚕丝绸中生产重组蛋白的方法。解决方案：产生具有GFP表达由丝胶蛋白基因启动子控制的转基因蚕。家蚕最后期幼虫丝腺的观察仅在中间丝腺中显示出荧光。还发现GFP从纺丝阶段周围的中间丝腺细胞分泌，GFP被移入腺体腔。最后，将GFP旋转成茧丝，产生含有大量GFP的茧。通过这种方式，发现通过利用丝胶蛋白基因的启动子区域可以在中间丝腺中产生重组的蛋白质，并且还发现在中间丝腺中产生的重组蛋白质可以容易地分泌到内腔中中间丝腺。版权所有（C）2013，JPO＆INPIT

8. 发明专利

JP2006137739A Method for producing proteins using silkworm middle silk gland-specific gene expression system 有权
标题翻译：使用丝绸中间丝绸特异性基因表达系统生产蛋白质的方法
公开(公告)号：JP2006137739A
公开(公告)日：2006-06-01
申请号：JP2005072401
申请日：2005-03-15
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： TAMURA TOSHIKI , SETSUTSU HIDEKI , KOBAYASHI ISAO , KOJIMA KATSURA , KANDA TOSHIO , UCHINO KEIRO
IPC分类号： C07K1/00 , A01K67/027 , C12N15/09
CPC分类号： C12N15/8509 , A01K67/0333 , A01K67/04 , A01K2217/05 , A01K2227/706 , A01K2267/01 , A01K2267/0393
摘要： PROBLEM TO BE SOLVED: To provide a method for producing recombination protein in the silkworm middle silk gland.
SOLUTION: Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silk gland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amount of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk gland. Further, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种在蚕丝绸中产生重组蛋白的方法。解决方案：产生其表达受丝胶蛋白基因启动子调节的包含GFP的转基因蚕。蚕的最后一龄幼虫丝腺的观察仅在中间丝腺中显示出荧光。 GFP从纺丝阶段的中间丝腺细胞分泌，表明GFP移入腺体腔。最后，将GFP转染成茧丝，生产含有大量GFP的茧。因此，通过使用丝胶蛋白基因的启动子区域，可以在中间丝腺中产生重组蛋白质。此外，在中间丝腺中产生的重组蛋白质容易分泌到中间丝腺的内腔中。版权所有（C）2006，JPO＆NCIPI

9. 发明专利

JP2010029102A α1,3-FUCOSYLTRANSFERASE 审中-公开
标题翻译： alpha1,3，岩藻糖基
公开(公告)号：JP2010029102A
公开(公告)日：2010-02-12
申请号：JP2008194338
申请日：2008-07-29
申请人： Katakura Industries Co Ltd , National Agriculture & Food Research Organization , National Institute Of Agrobiological Sciences , Osaka Univ , 国立大学法人大阪大学 , 片倉工業株式会社 , 独立行政法人農業・食品産業技術総合研究機構 , 独立行政法人農業生物資源研究所
发明人： NOMURA TAKESHI , ISHIYAMA SEIJI , USAMI AKIHIRO , FUJIYAMA KAZUHITO , SEKI TATSUJI , OKADA TAKAHIRO , SAITO TAKEO , YAMADA TOMOHIRO , TAMURA TOSHIKI , KOBAYASHI ISAO
IPC分类号： C12N15/09 , A01H1/00 , A01H5/00 , A01K67/02 , A01K67/027 , A61K38/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a protein to which a human sugar chain is added by an animal and vegetable protein production system for originally adding a nonhuman sugar chain by isolating and identifying α1,3-fucosyltransferase of Lepidoptera and Solanales related to the addition of the sugar chain to the protein, and suppressing the expression of the α1,3-fucosyltransferase in the Lepidoptera and the Solanales.
SOLUTION: The method for suppressing the activity of the α1,3-fucosyltransferase includes a step of introducing a DNA for suppressing the activity of the α1,3-fucosyltransferase in the Lepidoptera into a cell, and a step of expressing the DNA in the cell.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种生产通过动植物蛋白生产系统添加人糖链的蛋白质的方法，用于通过分离和鉴定鳞翅目α1,3-岩藻糖基转移酶来最初添加非人糖链和Solanales与向蛋白质中添加糖链有关，并抑制了鳞翅目和Solanales中α1,3-岩藻糖基转移酶的表达。解决方案：用于抑制α1,3-岩藻糖基转移酶的活性的方法包括将鳞翅目中的α1,3-岩藻糖基转移酶的活性抑制的DNA导入细胞的步骤，以及表达DNA 在细胞中版权所有（C）2010，JPO＆INPIT

10. 发明专利

JP2005143428A Utilization of dna encoding kynurenine oxidase gene of silkworm 有权
标题翻译：丝裂霉素氧化酶基因编码DNA的利用
公开(公告)号：JP2005143428A
公开(公告)日：2005-06-09
申请号：JP2003387764
申请日：2003-11-18
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： TAMURA TOSHIKI , KOBAYASHI ISAO , ZEN KUNIOKI , KANDA TOSHIO
IPC分类号： A01K67/033 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for utilizing DNA encoding kynurenine oxidase gene of silkworm as a selection marker for a transgenic silkworm. SOLUTION: A new vector plasmid to which a promoter of cytoplasmic actin of silkworm is linked is built on the upstream zone of kynurenine oxidase cDNA prepared from a wild silkworm in a vector derived from transposon piggyBac, and the vector plasmid is introduced into the egg of a white egg line silkworm obtained by making a silkworm of a first white egg mutation line (w-1) cross with a silkworm of a colored nondormant line (pnd). As a result, color of eyes of an adult moth or egg of a second-generation moth into which the vector is introduced is changed to blackish brown. The color of the skin of larva individual into which the gene is introduced is further changed to blackish brown and the change can be identified by naked eyes. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种利用编码蚕的犬尿苷氧化酶基因的DNA作为转基因蚕的选择标记的方法。解决方案：将来自转座子piggyBac的载体中的野生蚕制备的犬瘟氧化酶cDNA的上游区域建立在蚕胞质肌动蛋白启动子上的新载体质粒，并将载体质粒导入通过使第一个白蛋突变线（w-1）的蚕与一个有色的非休眠线（pnd）的蚕相交而获得的白蛋系蚕卵。结果，引入载体的第二代蛾的成年蛾或卵的眼睛颜色变为黑褐色。引入基因的幼虫个体的皮肤颜色进一步变为黑褐色，肉眼可以鉴定出变化。版权所有（C）2005，JPO＆NCIPI

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式