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1. 发明专利

JP2011147378A Method for producing dihydroxyacetone 有权
标题翻译：生产二羟基乙酮的方法
公开(公告)号：JP2011147378A
公开(公告)日：2011-08-04
申请号：JP2010010340
申请日：2010-01-20
申请人： National Institute Of Advanced Industrial Science & Technology , Yamaguchi Univ , 国立大学法人山口大学 , 独立行政法人産業技術総合研究所
发明人： HABE HIROSHI , FUKUOKA TOKUMA , KITAMOTO MASARU , SAKAKI KEIJI , MATSUSHITA KAZUNOBU , YAKUSHI HISAHARU , ANO YOSHITAKA
IPC分类号： C12P7/28 , C12N15/113 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently producing dihydroxyacetone from highly concentrated glycerol by a microbial reaction process.
SOLUTION: The method for producing the dihydroxyacetone includes culturing microorganisms in which at least one of genes concerning with glycerol metabolism is disrupted, in a glycerol-containing medium.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供通过微生物反应方法从高浓缩甘油有效地制备二羟基丙酮的方法。解决方案：制备二羟基丙酮的方法包括在含甘油的培养基中培养其中至少一个与甘油代谢有关的基因被破坏的微生物。版权所有（C）2011，JPO＆INPIT

2. 发明专利

JP2014064477A Breeding method of acetic acid bacterium improved production ability of acetic acid 审中-公开
标题翻译：乙酸的培育方法提高醋酸的生产能力
公开(公告)号：JP2014064477A
公开(公告)日：2014-04-17
申请号：JP2012210068
申请日：2012-09-24
申请人： Yamaguchi Univ , 国立大学法人山口大学
发明人： MATSUSHITA KAZUNOBU , YAKUSHI HISAHARU , MATSUTANI MINENOSUKE , NATSARAN SAICHANA , NISHIKURA MITSUTERU , GUNJANA THEERGOOL
IPC分类号： C12N1/21 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a breeding method of an acetic acid bacterium improved production ability of acetic acid which has growth ability and fermentation ability of acetic acid at high temperature.SOLUTION: A breeding method of an acetic acid bacterium comprises the steps of: acquiring gene involved in resistance ability of acetic acid, heat resistance and peroxidation of acetic acid in an acetic acid bacterium; modifying protein coded in the gene to reduce or delete a function of the protein, thereby improving production ability of acetic acid in an acetic acid bacterium; and breeding the acetic acid bacterium. Vinegar with high concentration of acetic acid is effectively produced by cultivating the acetic acid bacterium improved the production ability of acetic acid in a culture medium containing ethanol.
摘要翻译：要解决的问题：提供一种乙酸菌的提高醋酸生产能力的培养方法，其具有在高温下乙酸生长能力和发酵能力的乙酸生产能力。解决方案：乙酸菌的育种方法包括以下步骤：获得乙酸菌中乙酸抗性，耐热性和乙酸过氧化的基因; 修饰基因编码的蛋白质以减少或删除蛋白质的功能，从而提高乙酸细菌中乙酸的生产能力; 并培育乙酸菌。醋酸浓度高的醋通过培养乙酸菌有效地提高乙酸培养基中乙酸的生产能力。

3. 发明专利

JP2012131765A 4-keto-d-arabonic acid, 4-keto-d-arabinose, and production method of the same 审中-公开
标题翻译： 4-KETO-D-阿拉伯酸，4-KETO-D-ARABINOSE及其生产方法
公开(公告)号：JP2012131765A
公开(公告)日：2012-07-12
申请号：JP2011042459
申请日：2011-02-28
申请人： Yamaguchi Univ , 国立大学法人山口大学
发明人： ADACHI KAZUO , YAKUSHI HISAHARU , MATSUSHITA KAZUNOBU , ROQUE ALBERTO HOURS
IPC分类号： C07C49/185 , C07C59/215 , C12N1/20 , C12P7/42
摘要： PROBLEM TO BE SOLVED: To provide 4-keto-D-arabonic acid as a novel compound and its production method, and 4-keto-D-arabinose and its production method, which is the intermediate product of 4-keto-D-arabonic acid.SOLUTION: By cultivating 4-keto-D-arabonic acid-producing bacteria belonging to Gluconacetobacter or Gluconobacter, for example, cultivating Gluconacetobacter liquefaciens having ability of producing 4-keto-D-arabonic acid, in a cultivation medium containing D-glucose, D-gluconic acid or 2-keto-D-gluconic acid, 2,5-diketo-D-gluocnic acid (25DKA) is produced as a metabolite, which is further metabolized to produce 4-keto-D-arabonic acid. After isolating washed cells or a cytoplasmic membrane fraction of the Gluconacetobacter liquefaciens, when the washed cells or the cytoplasmic membrane fraction is made to react with 25DKA, not only 4-keto-D-arabonic acid but 4-keto-D-arabinose that is an intermediate product of 4-keto-D-arabonic acid can be produced.
摘要翻译：待解决的问题：提供作为新型化合物的4-酮-D-阿拉伯酸及其制备方法和4-酮-D-阿拉伯糖及其制备方法，其为4-酮 - D-阿拉伯酸。解决方案：通过培养属于葡糖乙酸杆菌属或葡萄糖酸杆菌属的4-酮-L-阿拉伯酸生产菌，例如培养含有D-酮酸葡萄糖醛酸的培养基中培养具有产生4-酮基-D-阿拉伯糖酸的能力的葡糖型醋酸杆菌属，葡萄糖，D-葡萄糖酸或2-酮-D-葡糖酸，2,5-二酮-D-葡萄糖酸（25DKA）作为代谢产物，进一步被代谢以产生4-酮-D-阿糖酸。在分离洗涤的细胞或液化葡糖醋酸杆菌的细胞质膜级分后，当洗涤的细胞或细胞质膜级分与25DKA反应时，不仅4-酮-D-阿拉伯糖，而且是4-酮-D-阿拉伯糖可以制备4-酮-L-阿拉伯酸的中间产物。版权所有（C）2012，JPO＆INPIT

4. 发明专利

JP2010110298A High temperature acetic acid fermentation acetic acid bacterium 有权
标题翻译：高温醋酸发酵醋酸菌
公开(公告)号：JP2010110298A
公开(公告)日：2010-05-20
申请号：JP2008287703
申请日：2008-11-10
申请人： Yamaguchi Univ , 国立大学法人山口大学
发明人： MATSUSHITA KAZUNOBU , HATANO TOMOYUKI , YAKUSHI HISAHARU , ADACHI KAZUO
IPC分类号： C12N1/20 , C12P7/54
摘要： PROBLEM TO BE SOLVED: To create a natural mutant having a growth and acetic acid fermentation ability even at 39 to 41°C, because the upper limit temperature of efficient acetic acid fermentation of known acetic acid bacteria is 38°C, and because installations and cost loads for cooling to ≤38°C are large.
SOLUTION: There is provided a mutant of acetic acid bacterium, Acetobacter pasteurianus, obtained by repeating the culture of Acetobacter pasteurianus SKU1108 at stepwisely rised culture temperatures to impart an ability for adapting to high temperatures.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：即使在39〜41℃下也可以生产具有生长和乙酸发酵能力的天然突变体，因为已知乙酸菌的有效乙酸发酵的上限温度为38℃，因为冷却至≤38°C的安装和成本负荷较大。解决方案：提供了一种乙酸菌，巴斯德黑醋杆菌的突变体，其通过在逐步升高的培养温度下重复培养巴斯德醋酸醋杆菌而获得，以赋予适应高温的能力。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2012184190A 4-keto-d-ribonic acid, 4-keto-d-ribose and method for producing them 审中-公开
标题翻译： 4-KETO-D-RIBONIC ACID，4-KETO-D-RIBOSE AND METHOD FOR PRODUCE THEM
公开(公告)号：JP2012184190A
公开(公告)日：2012-09-27
申请号：JP2011047632
申请日：2011-03-04
申请人： Yamaguchi Univ , 国立大学法人山口大学
发明人： ADACHI KAZUO , YAKUSHI HISAHARU , MATSUSHITA KAZUNOBU , ROQUE ALBERTO HOURS
IPC分类号： C07C49/185 , C07C59/215 , C07H3/02 , C07H7/027 , C12P7/58
摘要： PROBLEM TO BE SOLVED: To provide 4-keto-D-ribonic acid which is a novel compound, a method for producing the 4-keto-D-ribonic acid, 4-keto-D-ribose and a method for producing the 4-keto-D-ribose.SOLUTION: A 4-keto-D-ribonic acid producing microorganism and a 4-keto-D-ribose producing microorganism, for example, a living microorganism of Gluconobacter suboxydans having 4-keto-D-ribonic acid and 4-keto-D-ribose producing ability is crushed with a French press and then is subjected to centrifugal operation, the resulting precipitate is isolated as a cell membrane fraction, and thereafter, the cell membrane fraction is reacted with D-ribose to produce 4-keto-D-ribonic acid in addition to 4-keto-D-ribose as metabolic products.
摘要翻译：待解决的问题：提供作为新化合物的4-酮-D-核糖酸，制备4-酮-D-糖酸，4-酮-D-核糖的方法及其制备方法 4-酮-D-核糖。解决方案：产生4-酮-D-核糖的微生物和产生4-酮-D-核糖的微生物，例如，具有4-酮-D-核糖酸和4-酮基的葡糖酸杆菌的活微生物将D-核糖生成能力用法式压片机压碎，然后进行离心操作，分离所得沉淀物作为细胞膜级分，然后使细胞膜级分与D-核糖反应，生成4-酮基 - D-核糖酸除了作为代谢产物的4-酮-D-核糖外。版权所有（C）2012，JPO＆INPIT

6. 发明专利

JP2012125164A Gene defect method acetic acid bacterium not inserted with exogenous dna 审中-公开
标题翻译：基因缺陷方法不含有外源DNA的乙酸细菌
公开(公告)号：JP2012125164A
公开(公告)日：2012-07-05
申请号：JP2010277477
申请日：2010-12-13
申请人： Yamaguchi Univ , 国立大学法人山口大学
发明人： YAKUSHI HISAHARU , FUJII MASAKO , MATSUSHITA KAZUNOBU
IPC分类号： C12N15/09 , C12N1/19
摘要： PROBLEM TO BE SOLVED: To provide a method for making gene defect acetic acid bacteria capable of more highly effectively obtaining the gene defect acetic acid bacteria made without inserting the exogenous DNA to the genome DNA of the acetic acid bactera.SOLUTION: The method includes a step (a) for preparing a DNA segment including a sequence in which a target gene or homologous sequences of two areas sandwiching a part of the same are tandemly coupled for deleting the target gene in the acetic acid bacterium genome, a step (b) for preparing a cyclic DNA by introducing the DNA segment into a vector having a drug resistant gene and a marker gene and not having a replication starting point of the acetic acid bacteria, a step (c) for introducing the cyclic DNA into the acetic bacteria having the target gene, a step (d) for selecting by culturing the acetic acid bacteria in which the cyclic DNA whole length is assembled into the acetic acid genom by homologous recombination of the acetic acid bacteria to which the cyclic DNA is introduced by a culture medium containing drug corresponding to the drug resistant gene, and a step (e) for culturing the acetic acid bacteria provided with the drug resistance selected by the step (d) by a culture medium not containing the drug.
摘要翻译：要解决的问题：提供一种能够更加有效地获得不将外源DNA插入到乙酸菌的基因组DNA中而制备的基因缺陷型乙酸菌的基因缺陷型乙酸菌的方法。解决方案：该方法包括步骤（a），用于制备DNA片段，其包括其中靶基因或夹带其部分部分的两个区域的同源序列串联耦合的序列，以删除乙酸中的靶基因细菌基因组，通过将DNA片段导入具有耐药性基因的载体和标记基因而不具有乙酸菌的复制起始点的步骤（b），用于引入环状DNA的步骤（b）循环DNA进入具有靶基因的乙酸菌中，步骤（d）通过将其中将全长DNA整合到乙酸基因组中的乙酸菌通过将乙酸菌的同源重组通过含有与药物抗性基因相对应的药物的培养基导入环状DNA，和（e）培养具有步骤（d）选择的耐药性的乙酸菌的培养物不含药物的介质。版权所有（C）2012，JPO＆INPIT

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