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1. 发明专利

JP2009183195A Alkali-proof glutaminase 有权
标题翻译：阿尔卡利防腐谷氨酰胺酶
公开(公告)号：JP2009183195A
公开(公告)日：2009-08-20
申请号：JP2008025824
申请日：2008-02-06
申请人： National Institute Of Advanced Industrial & Technology , Taiyo Kagaku Co Ltd , 太陽化学株式会社 , 独立行政法人産業技術総合研究所
发明人： YOKOYAMA TAKUJI , KITAHATA KOICHI , NANBU HIRONOBU , YAMAZAKI YOSHIKI , ITO TETSUJI , ISHII AKIRA , HAMAKAWA SATOSHI , TSUNODA TATSURO , HANAOKA TAKAMASA , MIZUKAMI FUJIO
IPC分类号： C12N9/96 , C12N9/98
摘要： PROBLEM TO BE SOLVED: To provide alkali-proof glutaminase which can make glutaminase act very efficiently for the purpose of producing seasoned foods, etc.
SOLUTION: The alkali-proof glutaminase consists of a silica porous body having a pore diameter larger than 3 nm as an average pore size and 1-20 mass% of metal contents within the silica porous body, and glutaminase.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供能够使谷氨酰胺酶非常有效地用于生产经验证的食品等的耐碱性谷氨酰胺酶。解决方案：耐碱性谷氨酰胺酶由具有孔的二氧化硅多孔体组成直径大于3nm，平均孔径和1-20质量％的二氧化硅多孔体内的金属含量，以及谷氨酰胺酶。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2003286010A Conductive oxide mixture 有权
标题翻译：导电氧化物混合物
公开(公告)号：JP2003286010A
公开(公告)日：2003-10-07
申请号：JP2002088252
申请日：2002-03-27
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , KOBAYASHI KIYOSHI
IPC分类号： C04B35/50 , C01B13/02 , C01B13/18 , C01G3/00 , C01G9/00 , H01B1/08
摘要： PROBLEM TO BE SOLVED: To provide a conductive oxide mixture stably existing without causing embrittlement even if the transfer of oxygen is carried out, having the oxygen ion conductivity and the electron conductivity optionally controlled by the combination of oxides having different physical properties from each other or the change of composition ratio of the oxides to each other to be effectively used as a material giving a desired oxide ion permeation rate or a reactive material and having excellent sintering property, moldability, workability and the like. SOLUTION: The conductive oxide mixture formed from the combination of an oxide having oxide ion conductivity and an oxide having electron conductivity which exists as a mixed structural phase being in a thermodynamic equilibrium state is provided. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：为了提供即使进行氧的转移也能稳定存在而不引起脆化的导电氧化物混合物，具有氧离子传导性和电子传导性，任选地通过具有不同物理性质的氧化物的组合来控制彼此之间或氧化物的组成比的变化相互有效地用作具有所需氧化物离子渗透速率的材料或反应性材料，并且具有优异的烧结性，成型性，可加工性等。解决方案：提供由具有氧化物离子传导性的氧化物和具有作为混合结构相存在的具有电子传导性的氧化物的组合形成的导电氧化物混合物处于热力学平衡状态。版权所有（C）2004，JPO

3. 发明专利

JP2014103924A Dna synthetase-silica-based nanoporous material composite, method of producing the same, and use thereof 有权
标题翻译： DNA合成二氧化硅基纳米材料复合材料，其生产方法及其用途
公开(公告)号：JP2014103924A
公开(公告)日：2014-06-09
申请号：JP2012260391
申请日：2012-11-28
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所 , Research Organization Of Information & Systems , 大学共同利用機関法人情報・システム研究機構
发明人： MATSUURA SHUNICHI , CHIBA MANAMI , TSUNODA TATSURO , BABA TOMOYA
IPC分类号： C12N11/14 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a DNA synthetase-silica-based nanoporous material composite, a method of producing the same, and a use thereof.SOLUTION: There is provided a DNA synthetase-silica-based nanoporous material composite which is an enzyme-containing composite having a DNA synthetase with an ability to synthesize DNA in silica pores of a silica-based nanoporous material, in which an enzyme is stably fixed in the silica pores of the silica-based nanoporous material before and after enzyme reaction, and which is in a composite state in which the enzyme-containing composite exhibits interaction with a nucleic acid (DNA) which is a reaction substrate and can stably exhibit enzyme activity. There are provided a DNA synthetase-containing high-durability member which consists of the DNA synthetase-silica-based nanoporous material composite and which can stably adsorb, fix, and hold the enzyme on the silica pores of the silica-based nanoporous material and can stably exhibit the ability to synthesize DNA even when it is repeatedly used for DNA synthesis reaction, and a method of amplifying DNA from an infinitesimal sample by the DNA synthetase-silica-based nanoporous material composite.
摘要翻译：要解决的问题：提供DNA合成酶 - 二氧化硅基纳米多孔材料复合物及其制备方法及其用途。提供了一种DNA合成酶 - 二氧化硅基纳米多孔材料复合材料，含有能够在二氧化硅系纳米多孔材料的二氧化硅孔中合成DNA的DNA合成酶的复合物，其中酶稳定地固定在二氧化硅系纳米多孔材料的二氧化硅孔内，在酶反应前后，处于复合状态，其中含酶复合物与作为反应底物的核酸（DNA）显示相互作用并且可以稳定地显示酶活性。提供了含有DNA合成酶的高耐久性构件，其由DNA合成酶 - 二氧化硅基纳米多孔材料复合材料组成，可以稳定地吸附，固定并保持酶在二氧化硅基纳米多孔材料的二氧化硅孔上，并且可以即使在重复使用DNA合成反应时也稳定地表现出合成DNA的能力，以及通过DNA合成酶 - 二氧化硅基纳米多孔材料复合体从无限小样品中扩增DNA的方法。

4. 发明专利

JP2009112242A α-AMYLASE-FSM BASED MESO PORE POROUS COMPLEX 审中-公开
标题翻译：基于α-AMYLASE-FSM的MESO PORE POROUS COMPLEX
公开(公告)号：JP2009112242A
公开(公告)日：2009-05-28
申请号：JP2007288426
申请日：2007-11-06
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , HISAMATSU KANAKO , TOGASHI HIDEAKI , NARA TAKAYUKI , SEKIKAWA CHISATO , KAWAI AKIKO , SHIOMI TORU , MIZUKAMI FUJIO , SAKAGUCHI KENGO
IPC分类号： C12N11/14
摘要： PROBLEM TO BE SOLVED: To provide an amylase-FSM based meso pore porous complex which contributes to realization of efficient starch hydrolysis of various starch raw materials, and facilitates recovery, recycling and continuous utilization of the enzyme. SOLUTION: Provided are the amylase-FSM based meso pore porous complex having a structure in which the amylase is immobilized in the FSM based meso pore porous, and the immobilized amylase has an enzymatic activity to catalyze the hydrolysis of starch; a method for producing the amylase-FSM based meso pore porous complex, comprising adsorption-holding and immobilizing the amylase to the FSM based meso pore porous; and a method for producing the hydrolyzate of the starch with the complex. According to the present invention, further provided are the amylase-FSM based meso pore porous complex in which the enzyme is immobilized in a highly integrated state, the method for producing the same, and the process for hydrolyzing the starch with the complex. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种淀粉酶-FMM的中孔多孔复合物，其有助于实现各种淀粉原料的有效淀粉水解，并促进酶的回收，再循环和连续利用。解决方案：提供了具有淀粉酶固定在基于FSM的中孔多孔的结构的基于淀粉酶-FMM的中孔多孔复合物，并且固定的淀粉酶具有催化淀粉水解的酶活性; 一种生产淀粉酶-FSM的中孔多孔复合物的方法，包括将淀粉酶吸附保持并固定在基于FSM的中孔多孔中; 以及用复合物生产淀粉的水解产物的方法。根据本发明，进一步提供了以高度一体化的状态固定酶的淀粉酶-FMM的中孔多孔复合物，其制备方法以及用复合物水解淀粉的方法。版权所有（C）2009，JPO＆INPIT

5. 发明专利

JP2009038984A Thermostable glutaminase 审中-公开
标题翻译：热稳定性谷氨酰胺酶
公开(公告)号：JP2009038984A
公开(公告)日：2009-02-26
申请号：JP2007204555
申请日：2007-08-06
申请人： National Institute Of Advanced Industrial & Technology , Taiyo Kagaku Co Ltd , 太陽化学株式会社 , 独立行政法人産業技術総合研究所
发明人： KITAHATA KOICHI , NANBU HIRONOBU , YAMAZAKI YOSHIKI , ITO TETSUJI , ISHII AKIRA , HAMAKAWA SATOSHI , TSUNODA TATSURO , HANAOKA TAKAMASA , MIZUKAMI FUJIO
IPC分类号： C12N9/78 , C12N11/14
摘要： PROBLEM TO BE SOLVED: To provide a thermostable glutaminase enabling the glutaminase to act extremely efficiently for the purpose of the production of a seasoned food or the like.
SOLUTION: The thermostable glutaminase comprises a porous body of silica having >8 nm pore diameter, and the glutaminase.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种热稳定的谷氨酰胺酶，使得谷氨酰胺酶能够非常有效地起作用以生产经验丰富的食品等。解决方案：热稳定性谷氨酰胺酶包括具有> 8nm孔径的二氧化硅多孔体和谷氨酰胺酶。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2008143892A Silica-based mesoporous material-heteroprotein complex and method for producing the same 有权
标题翻译：二氧化硅基异种材料复合物及其制备方法
公开(公告)号：JP2008143892A
公开(公告)日：2008-06-26
申请号：JP2007297308
申请日：2007-11-15
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： MATSUURA SHUNICHI , MIZUKAMI FUJIO , ITO TETSUJI , ISHII AKIRA , TSUNODA TATSURO , HANAOKA TAKAMASA , SAKAGUCHI KENGO
IPC分类号： C07K17/14 , C01B37/02 , C07K14/435 , C12N11/14 , G01N21/78 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a silica-based mesoporous material-heteroprotein inclusion complex and to provide a method for producing the complex and to provide application of the complex.
SOLUTION: The silica-based mesoporous body-heteroprotein complex has at least two kinds of heteroproteins different in properties in pore interior of a silica-based mesoporous body (FSM: folded-sheet mesoporous material). The complex is characterized in that the proteins are absorbed in the pores of the FSM and stably fixed in a dispersed state and two kinds of heteroproteins are kept in a close state so as to exhibit mutual action between the heteroproteins and the proteins are absorbed in pores of the FSM as proteins accumulated in high density. The method for producing the complex is also provided. A functional member is composed of the complex and the member is obtained by stably absorbing a heteroprotein having functionality into pores of FSM in a disperse state and stably retaining the activity. According to the present invention, new FSM-heteroprotein complex in which activity of heteroprotein having functionality such as fluorescent property or luminescence is stably retained, a method for producing the complex and application as the functional member can also be provided.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供二氧化硅基介孔材料 - 异蛋白包合物并提供复合物的制备方法并提供复合物的应用。解决方案：二氧化硅基介孔体 - 异型蛋白复合物在二氧化硅基介孔体（FSM：折叠片介孔材料）的孔内具有至少两种不同性质的异质蛋白。该复合物的特征在于蛋白质被吸收在FSM的孔中，并以分散的状态稳定地固定，并且两种异蛋白保持在接近状态，以便在杂蛋白之间发挥相互作用，并且蛋白质被吸收在孔中的FSM作为高密度积聚的蛋白质。还提供了生产复合物的方法。功能性构件由络合物组成，通过在分散状态下稳定地吸收具有官能团的功能性的蛋白质的孔而获得，并稳定地保持活性。根据本发明，还可以提供其中稳定保留具有诸如荧光性质或发光等功能的杂蛋白的活性的新的FSM-异型蛋白复合物，制备复合物的方法和用作功能性构件。版权所有（C）2008，JPO＆INPIT

7. 发明专利

JP2003300708A Precursor composition for mixture conductive oxide 有权
标题翻译：混合导电氧化物的前驱体组合物
公开(公告)号：JP2003300708A
公开(公告)日：2003-10-21
申请号：JP2002100987
申请日：2002-04-03
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , KOBAYASHI KIYOSHI
IPC分类号： B01D53/22 , B01D71/02 , C01B13/32 , C01G29/00 , H01B1/06
摘要： PROBLEM TO BE SOLVED: To provide a precursor composition from which a uniform and dense mixture conductive oxide with excellent low temperature sintering property, molding property, processing property, or the like, is obtained and in which a composition of an ionic conductive oxide and an electron conductive metal in the mixture conductive oxide can be properly, easily and accurately controlled, and to provide the conductive oxide having a mixture structural phase obtained from the above composition.
SOLUTION: The precursor composition for the mixture conductive oxide comprises a metal salt having oxygen ionic conductivity, a noble metal salt having electron conductivity, a metal chelating agent and a solidifying agent. A mixture conductive oxide sintered body is obtained from the above precursor composition and comprises the combination of an oxide having oxygen ionic conductivity and a metal having electron conductivity, in which the oxide having the oxygen ionic conductivity and the metal having the electron conductivity are present as the mixture structural phase in a thermodynamic equilibrium state. The sintered body is useful as an oxygen permeation material or oxygen enriched material.
COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供一种前体组合物，其中获得具有优异的低温烧结性能，成型性能，加工性能等的均匀致密混合物导电氧化物，其中离子导电氧化物和导电性氧化物中的电子传导性金属可以适当，容易且准确地控制，并提供具有由上述组成获得的混合结构相的导电氧化物。解决方案：用于混合物导电氧化物的前体组合物包括具有氧离子导电性的金属盐，具有电子传导性的贵金属盐，金属螯合剂和固化剂。由上述前体组合物获得混合导电氧化物烧结体，并且包括具有氧离子传导性的氧化物和具有电子传导性的金属的组合，其中具有氧离子导电性的氧化物和具有电子传导性的金属以混合结构相处于热力学平衡状态。烧结体可用作氧气渗透材料或富氧材料。版权所有（C）2004，JPO

8. 发明专利

JP2009153448A Hydrolase complex of silica-based meso porous body-starch 审中-公开
标题翻译：基于二氧化硅的MESO多孔体系的水解复合物
公开(公告)号：JP2009153448A
公开(公告)日：2009-07-16
申请号：JP2007335229
申请日：2007-12-26
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , TOGASHI HIDEAKI , SEKIKAWA CHISATO , NARA TAKAYUKI , HISAMATSU KANAKO , SHIOMI TORU , KAWAI AKIKO , MIZUKAMI FUJIO , SAKAGUCHI KENGO
IPC分类号： C12N11/14 , C12P19/14
摘要： PROBLEM TO BE SOLVED: To provide a hydrolase complex of silica-based meso porous body-starch, which contributes to the realization of efficient starch hydrolysis for various starch materials, and enables the recovery, recycling and continuous use of the enzyme.
SOLUTION: Provided is the hydrolase complex of silica-based meso porous body-starch, which is the complex of the silica-based meso porous body with the enzyme for hydrolyzing the starch, characterized in that the enzyme immobilized on the silica-based meso porous body has activity for catalyzing the hydrolysis of starch. Provided is a method for producing the hydrolase complex of silica-based meso porous body-starch, characterized by mixing the silica-based meso porous body with a solution containing the hydrolase to stably adsorb, hold and immobilize the hydrolase on the silica-based meso porous body and in the fine pores of the silica-based meso porous body. Further, provided is a method for producing a starch hydrolysis product, characterized by hydrolyzing starch with the hydrolase complex of silica-based meso porous body-starch.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供二氧化硅基中孔多孔体淀粉的水解酶复合物，其有助于实现各种淀粉材料的有效淀粉水解，并且能够回收，再循环和连续使用酶。解决方案：提供二氧化硅基的内消旋多孔体 - 淀粉的水解酶复合物，其是二氧化硅基的内消旋多孔体与用于水解淀粉的酶的复合物，其特征在于固定在二氧化硅 - 基于meso多孔体具有催化淀粉水解的活性。本发明提供一种二氧化硅系内消旋多孔体淀粉的水解酶配合物的制造方法，其特征在于，将二氧化硅系中孔多孔体与含有水解酶的溶液混合，使水解酶稳定地吸附，多孔体和二氧化硅基中孔多孔体的细孔内。此外，提供一种生产淀粉水解产物的方法，其特征在于用二氧化硅基的内消旋多孔体淀粉的水解酶复合物水解淀粉。版权所有（C）2009，JPO＆INPIT

9. 发明专利

JP2009126829A Method for activation-recovery of gfp (green fluorescent protein) function 有权
标题翻译： GFP（绿色荧光蛋白）功能的激活恢复方法
公开(公告)号：JP2009126829A
公开(公告)日：2009-06-11
申请号：JP2007303835
申请日：2007-11-24
申请人： Fujifilm Corp , National Institute Of Advanced Industrial & Technology , 富士フイルム株式会社 , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , SEKIKAWA CHISATO , NARA TAKAYUKI , TOGASHI HIDEAKI , SAKAGUCHI KENGO , MIZUKAMI FUJIO , KAWAKAMI MASAYUKI
IPC分类号： C07K14/435
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide activation-recovery of GFP function using a β type zeolite-containing refolding buffer. SOLUTION: The method for activation-recovery of GFP (green fluorescent protein) function comprises adding a β type zeolite-containing refolding buffer to GFP, incubating the GFP at a fixed temperature for a fixed time to carry out GFP refolding and activation-recovery of GFP function. The method for producing an active GFP comprises adapting the method for an activation-recovery of GFP function to an inactive or low active GFP to recover an active GFP having activation-recovery of function. Consequently, a new method for activation-recovery of function of modified GFP using a β type zeolite can be established and provided. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：使用含β型沸石的重折叠缓冲液提供GFP功能的活化恢复。解决方案：GFP（绿色荧光蛋白）功能的活化恢复方法包括向GFP中加入含β型沸石的重折叠缓冲液，将GFP在固定温度下孵育固定时间以进行GFP重折叠和活化 - 恢复GFP功能。用于产生活性GFP的方法包括将用于将GFP功能的活化 - 恢复的方法适配到无活性或低活性GFP，以恢复具有活化恢复功能的活性GFP。因此，可以建立并提供使用β型沸石的修饰GFP的功能的活化恢复的新方法。版权所有（C）2009，JPO＆INPIT

10. 发明专利

JP2009112243A Method for activating and recovering function of lysozyme 有权
标题翻译：激活和恢复LYSOZYME功能的方法
公开(公告)号：JP2009112243A
公开(公告)日：2009-05-28
申请号：JP2007288448
申请日：2007-11-06
申请人： Fujifilm Corp , National Institute Of Advanced Industrial & Technology , 富士フイルム株式会社 , 独立行政法人産業技術総合研究所
发明人： TSUNODA TATSURO , NARA TAKAYUKI , TOGASHI HIDEAKI , SEKIKAWA CHISATO , MIZUKAMI FUJIO , SAKAGUCHI KENGO , KAWAKAMI MASAYUKI
IPC分类号： C12N9/14
摘要： PROBLEM TO BE SOLVED: To provide a method for activating and recovering the functions of lysozyme.
SOLUTION: This method for activating and recovering the functions of the lysozyme comprises adding a refolding buffer containing β-type zeolite, an oxidation-reduction agent such as cysteine, and guanidine hydrochloride to the lysozyme, and then incubating the mixture at a prescribed temperature for a certain time period. A method for producing active lysozyme comprises applying the method to inactive or low active lysozyme to recover the active lysozyme having activated and recovered functions.
COPYRIGHT: (C)2009,JPO&INPIT

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