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1. 发明专利

JP2004350509A New haloperoxidase and method for producing the same 审中-公开
标题翻译：新的卤代氧化酶及其生产方法
公开(公告)号：JP2004350509A
公开(公告)日：2004-12-16
申请号：JP2003148607
申请日：2003-05-27
申请人： Marine Biotechnol Inst Co Ltd , 株式会社海洋バイオテクノロジー研究所
发明人： IDE TERUHIKO , HAKU SOKO , MISAWA NORIHIKO
IPC分类号： C12N15/09 , C12N9/08 , C12P1/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To isolate a new haloperoxidase to provide a means for efficiently producing halogenated organic compounds having antibacterial activities and other various physiological activities. SOLUTION: This haloperoxidase is obtained from Pseudomonas putida, has a mol. wt. of about 30 kDa, and has a glycine serine motif. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：分离新的卤代过氧化物酶，以提供有效生产具有抗菌活性和其它各种生理活性的卤代有机化合物的方法。解决方案：该卤代过氧化物酶是从恶臭假单胞菌获得的，具有一个mol。重量。约30kDa，并具有甘氨酸丝氨酸基序。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2005065618A Gene encoding p450 useful for conversion of alkane 审中-公开
标题翻译：基因编码P450可用于转化ALKANE
公开(公告)号：JP2005065618A
公开(公告)日：2005-03-17
申请号：JP2003301294
申请日：2003-08-26
申请人： Marine Biotechnol Inst Co Ltd , 株式会社海洋バイオテクノロジー研究所
发明人： HAKU SOKO , HARA AKIHIRO , MISAWA NORIHIKO
IPC分类号： C12N15/09 , C12N1/21 , C12P7/04
摘要： PROBLEM TO BE SOLVED: To provide a gene encoding an enzyme capable of adding oxygen to a 10-14C straight-chain alkane. SOLUTION: P450 monooxygenase gene is obtained from Alcanivorax borkumensis DSM 11573 strain and encodes 471 amino acids. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供编码能够向10-14C直链烷烃加氧的酶的基因。解决方案：P450单加氧酶基因获自阿尔卡氏木霉DSM 11573菌株，编码471个氨基酸。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2004350510A Method for isolating new haloperoxidase gene 审中-公开
公开(公告)号：JP2004350510A
公开(公告)日：2004-12-16
申请号：JP2003148640
申请日：2003-05-27
申请人： Marine Biotechnol Inst Co Ltd , 株式会社海洋バイオテクノロジー研究所
发明人： IDE TERUHIKO , HAKU SOKO , MISAWA NORIHIKO
IPC分类号： C12N15/09 , C12N9/08 , C12P7/26 , C12P17/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a means for efficiently isolating a haloperoxidase gene from various microorganism resources without passing through a culture process. SOLUTION: This method for isolating the haloperoxidase gene is characterized by amplifying the fragment of a haloperoxidase gene contained in a sample with a primer designed on an amino acid sequence region highly retained among a plurality of haloperoxidases, and producing a hybrid gene from the fragment and a known haloperoxidase gene. COPYRIGHT: (C)2005,JPO&NCIPI

4. 发明专利

JP2003310274A Glucose dehydrogenase and gene encoding the same 审中-公开
标题翻译：葡萄糖脱氢酶和编码其的基因
公开(公告)号：JP2003310274A
公开(公告)日：2003-11-05
申请号：JP2002128309
申请日：2002-04-30
申请人： Amano Enzyme Inc , Marine Biotechnol Inst Co Ltd , 天野エンザイム株式会社 , 株式会社海洋バイオテクノロジー研究所
发明人： NAKANISHI YUJI , HAKU SOKO , KAGAMI OSAMU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04
摘要： PROBLEM TO BE SOLVED: To obtain a glucose dehydrogenase that has conventionally unknown resistance to heat and alkalis and/or improved specific activity by the genetic engineering and to provide methods for producing a recombinant vector, a transformant, and the glucose dehydrogenase by using the recombinant and to provide a glucose assaying kit. SOLUTION: The glucose dehydrogenase contains a specific amino acid sequence or an amido acid sequence from/for/to which one or several amino acids may be deleted, substituted or added and has resistance to heat and alkalis and/or improved specific activity. Further, a gene encoding these glucose dehydrogenase, genes each having the specific base sequence, recombinant vectors containing these genes, and the collection of glucose dehydrogenase that is produced by culturing the transformant and glucose assaying kit are provided. COPYRIGHT: (C)2004,JPO
摘要翻译：待解决的问题：为了获得具有传统上对热和碱耐受性的葡萄糖脱氢酶和/或通过遗传工程提高的比活性，并提供生产重组载体，转化体和葡萄糖脱氢酶的方法，通过使用重组体并提供葡萄糖测定试剂盒。解决方案：葡萄糖脱氢酶含有一个或几个氨基酸可以缺失，取代或加入的特定氨基酸序列或酰胺酸序列，并具有耐热和碱性和/或改善的比活性。此外，提供了编码这些葡萄糖脱氢酶的基因，各自具有特异碱基序列的基因，含有这些基因的重组载体以及通过培养转化体和葡萄糖测定试剂盒产生的葡萄糖脱氢酶的收集。版权所有（C）2004，JPO

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