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1. 发明专利

JP2003325200A New high-sensitive method for assaying nucleic acid 审中-公开
标题翻译：用于测定核酸的新型高灵敏度方法
公开(公告)号：JP2003325200A
公开(公告)日：2003-11-18
申请号：JP2003061958
申请日：2003-03-07
申请人： Kazuko Matsumoto , 和子松本
发明人： MATSUMOTO KAZUKO , EN HIROTOSHI
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/533
CPC分类号： C12Q1/6818 , C12Q1/6827 , C12Q2563/137 , C12Q2561/12 , C12Q2561/109
摘要： PROBLEM TO BE SOLVED: To provide a high-sensitive method for assaying SNP, specifically to provide the method for assaying the SNP by which a large amount of the SNP is rapidly assayed by a small amount of a DNA of a test object. SOLUTION: The method for assaying the SNP by using an invader method using a FRET (Fluorescence Resonance Energy Transfer) probe having a luminous coloring matter and a quenching material uses a labeling agent of a rare earth fluorescent complex comprising a rare earth element such as europium and terbium as the luminous coloring matter in the FRET probe. The fluorescence-regulating composition comprises a combination of the specific labeling agent of the rare earth fluorescent complex and the specific labeling agent of a fluorescence quencher. The high-sensitive labeling probe uses the labeling agent of the rare earth fluorescent complex as the luminous coloring matter in the high-sensitive labeling probe such as the FRET probe, and the labeling agent of the fluorescence quencher as the quenching material. The kit for assaying the SNP includes the high-sensitive labeling probe. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供用于测定SNP的高灵敏度方法，特别是提供用于测定SNP的方法，其中通过少量测试对象的DNA快速测定大量的SNP 。解决方案：使用具有发光色素和猝灭材料的FRET（荧光共振能量转移）探针的入侵者方法测定SNP的方法使用包含稀土元素的稀土荧光复合物的标记试剂如铕和铽作为FRET探针中的发光色素。荧光调节组合物包含稀土荧光复合物的特异性标记试剂和荧光猝灭剂的特异性标记试剂的组合。高灵敏度标记探针使用稀土荧光复合物的标记剂作为高灵敏度标记探针中的发光色素，如FRET探针，荧光猝灭剂的标记试剂作为淬火材料。用于测定SNP的试剂盒包括高灵敏度的标记探针。版权所有（C）2004，JPO

2. 发明专利

JP2007020526A Method for highly sensitively detecting target molecule by utilization of specific bond, and kit therefor 审中-公开
标题翻译：通过使用特定粘结剂敏感检测目标分子的方法及其制备方法
公开(公告)号：JP2007020526A
公开(公告)日：2007-02-01
申请号：JP2005210667
申请日：2005-07-20
申请人： Japan Science & Technology Agency , Kazuko Matsumoto , 和子松本 , 独立行政法人科学技術振興機構
发明人： SHA TOSHITAMA , FUKUI KOICHI , HORIE SUKENORI , KAGEYAMA YOSHITAKA , MATSUMOTO KAZUKO
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/532
CPC分类号： C12Q1/6818 , C12Q2561/109 , C12Q2525/301 , C12Q2521/301
摘要： PROBLEM TO BE SOLVED: To provide a label which can highly sensitively, regularly and stably be measured in excellent operability; to provide a labeling method using the same; and to provide an analysis method using the same. SOLUTION: This method for detecting, identifying or determining a target molecule in a specimen comprises using a chemical substance-bound oligonucleotide as a label, and then cleaving a nucleic acid with a nuclease to detect the oligonucleotide, in detail, cleaving the nucleic acid with a flap end nuclease, in more detail, by an invader method. Preferably, the method for detecting, identifying or determining the target molecule in the specimen comprises bringing a chemical substance (hereinafter referred to as a labeled chemical substance) bound to an oligonucleotide as a label into contact with a specimen which contains or may contain a target molecule, to form a complex of the labeled chemical substance with a target molecule in the specimen, and then measuring the oligonucleotide in the complex by the invader method. The labeled chemical substance, the oligonucleotide for labeling and the measurement kit therefor are provided for the above method. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供可以高度灵敏，定期和稳定地测量具有良好可操作性的标签; 提供使用该标签的标签方法; 并提供使用该方法的分析方法。解决方案：用于检测，鉴定或确定样品中靶分子的方法包括使用化学物质结合的寡核苷酸作为标记，然后用核酸酶切割核酸以检测寡核苷酸，详细地说，具有襟翼末端核酸酶的核酸，更详细地，通过入侵者方法。优选地，用于检测，鉴定或确定样品中的靶分子的方法包括将与寡核苷酸结合的化学物质（以下称为标记的化学物质）作为标记物与含有或可含有靶标的样品接触分子，在标本中形成标记化学物质与靶分子的复合物，然后通过侵入法测定复合物中的寡核苷酸。标记的化学物质，用于标记的寡核苷酸及其测定试剂盒用于上述方法。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2004132795A Method for measuring concentration of compound containing phosphoric acid or phosphate radical 审中-公开
标题翻译：测量含有磷酸或磷酸盐的化合物浓度的方法
公开(公告)号：JP2004132795A
公开(公告)日：2004-04-30
申请号：JP2002296787
申请日：2002-10-09
申请人： Kazuko Matsumoto , Toyobo Co Ltd , 東洋紡績株式会社 , 松本和子
发明人： TSUJI KATSUMI , NISHIYA YOSHIAKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12Q1/68 , G01N21/78
摘要： PROBLEM TO BE SOLVED: To provide a method for simply determining the concentration of a compound containing a phosphoric acid or a phosphate radical in an organism by measuring fluorescence.
SOLUTION: In the method for measuring the concentration of the compound containing the phosphoric acid or the phosphate radical, the fluorescence is measured. The fluorescence is emitted by a complex of a fluorescent substance expressed by the following general expression (I):Ln-S-N and a lanthanoid metal ion. In the general expression, Ln, S, and N represent 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedione-6"-yl)chlorosulfo-o-terphenyl or its derivative, a spacer molecule, and a nucleotide, respectively.
COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供通过测量荧光简单地测定生物体中含有磷酸或磷酸根的化合物的浓度的方法。解决方案：在测量含有磷酸或磷酸根的化合物的浓度的方法中，测量荧光。由以下通式（I）表示的荧光物质的复合物发射荧光：Ln-S-N和镧系金属离子。在通式中，Ln，S和N表示4,4'-双（1“，1”，1“，2”，2“，3”，3“ - 三氟-4”，6“ 6“ - 基）氯代邻 - 三联苯或其衍生物，间隔子分子和核苷酸。版权所有（C）2004，JPO

4. 发明专利

JP2004132796A Fluorescence measuring method 审中-公开
公开(公告)号：JP2004132796A
公开(公告)日：2004-04-30
申请号：JP2002296789
申请日：2002-10-09
申请人： Kazuko Matsumoto , Toyobo Co Ltd , 東洋紡績株式会社 , 松本和子
发明人： TSUJI KATSUMI , NISHIYA YOSHIAKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/533 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a method highly sensitive analysis method of a gene by increasing detection sensitivity of a fluorescent substance and a nucleic acid labelled by the fluorescent substance.
SOLUTION: In the method for measuring fluorescence, a surface-active agent is allowed to coexist in measurement. The fluorescence is emitted by a complex of a fluorescent substance expressed by the following general expression (I):Ln-S-N and a lanthanoid metal ion. In the general expression, Ln, S, and N are set to be 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedione-6"-yl)chlorosulfo-o-terphenyl or its derivative, a spacer molecule, and a nucleotide, respectively.
COPYRIGHT: (C)2004,JPO

5. 发明专利

JP2004129554A Method for fluorescent labeling 审中-公开
标题翻译：荧光标记方法
公开(公告)号：JP2004129554A
公开(公告)日：2004-04-30
申请号：JP2002296788
申请日：2002-10-09
申请人： Kazuko Matsumoto , Toyobo Co Ltd , 東洋紡績株式会社 , 松本和子
发明人： TSUJI KATSUMI , NISHIYA YOSHIAKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/53 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a method for fluorescent labeling, with which the efficiency of a method for nucleic acid labeling by a fluorescent substance is increased and thereby a high-sensitivity analysis method is provided.
SOLUTION: The method for nucleic acid labeling by taking a fluorescent substance represented by general formula (I) Ln-S-N (Ln is 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)chlorosulfo-o-terphenyl or its derivative; S is a spacer molecule; N is a nucleotide) in a nucleotide chain, maks a surfactant coexist in a reaction solution.
COPYRIGHT: (C)2004,JPO
摘要翻译：待解决的问题：提供荧光标记方法，通过该方法提高荧光物质的核酸标记方法的效率，从而提供高灵敏度分析方法。（I）Ln-SN（Ln是4,4'-双（1“，1”，1“，2”，2“）的荧光物质进行核酸标记的方法，3“，3” - 六氟-4“，6” - 己二酸-6“ - 基）氯磺基邻三联苯或其衍生物; S为间隔分子; N为核苷酸链中的核苷酸），表面活性剂并存在反应解决方案中。版权所有（C）2004，JPO

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