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1. 发明专利

JP2011147447A Method of expressing gene in transgenic bird using retrovirus vector and transgenic bird thus obtained 有权
标题翻译：使用回旋转录病毒和转基因获得的转基因基因表达方法
公开(公告)号：JP2011147447A
公开(公告)日：2011-08-04
申请号：JP2011040380
申请日：2011-02-25
申请人： Kaneka Corp , Nagoya Industrial Science Research Inst , 株式会社カネカ , 財団法人名古屋産業科学研究所
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI , ONO KENICHIRO
IPC分类号： A01K67/027 , C07K16/02 , C07K16/28 , C12N15/09 , C12N15/13 , C12N15/85
CPC分类号： A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C07K16/02 , C07K16/2806 , C07K2317/21 , C07K2317/52 , C07K2317/622 , C07K2319/00 , C12N15/8509 , C12N2799/027 , C12N2830/00 , C12N2830/15 , C12N2830/60 , C12N2830/90 , C12N2840/20 , C12N2840/203
摘要： PROBLEM TO BE SOLVED: To provide a G0 transgenic chimera bird which produces an antibody.
SOLUTION: There are provided: a G0 transgenic chimera bird in which an antibody gene is introduced with a replication-defective retrovirus vector, and which produces an antibody by expressing the transgene; a production method for an antibody, which comprises recovering the antibody expressed by the produced G0 transgenic chimera bird; and a production method for a G0 transgenic chimera bird, which comprises incubating a bird fertilized egg and injecting a retrovirus vector into the fertilized egg excluding immediately after the initiation of the incubation.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供产生抗体的G0转基因嵌合体鸟。提供：G0转基因嵌合体鸟，其中抗体基因与复制缺陷型逆转录病毒载体一起引入，并通过表达转基因产生抗体; 一种抗体的制备方法，其包括回收由所产生的G0转基因嵌合体鸟表达的抗体; 以及G0转基因嵌合体鸟的生产方法，其包括孵育鸟受精卵并将逆转录病毒载体注射到受精卵中，其中不包括在孵育开始后立即排除。版权所有（C）2011，JPO＆INPIT

2. 发明专利

JP2013255521A Method of expressing gene in transgenic birds using retrovirus vector and transgenic birds thus obtained 有权
标题翻译：使用返回型载体和转基因载体在转基因中表达基因的方法
公开(公告)号：JP2013255521A
公开(公告)日：2013-12-26
申请号：JP2013207597
申请日：2013-10-02
申请人： Kaneka Corp , 株式会社カネカ , Nagoya Industrial Science Research Institute , 公益財団法人名古屋産業科学研究所
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI , ONO KENICHIRO
IPC分类号： C12P21/08 , A01K67/027 , C07K16/02 , C07K16/28 , C12N15/09 , C12N15/13 , C12N15/85
CPC分类号： A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C07K16/02 , C07K16/2806 , C07K2317/21 , C07K2317/52 , C07K2317/622 , C07K2319/00 , C12N15/8509 , C12N2799/027 , C12N2830/00 , C12N2830/15 , C12N2830/60 , C12N2830/90 , C12N2840/20 , C12N2840/203
摘要： PROBLEM TO BE SOLVED: To provide a G0 transgenic chimera bird producing an antibody.SOLUTION: There are provided G0 transgenic chimeric birds which have an antibody gene having been transferred thereinto by using a retrovirus vector with a lack of replicability and produce the antibody by expressing the transferred gene; a process for producing an antibody by collecting the antibody express ed by the constructed G0 transgenic chimeric birds; and a method of constructing G0 transgenic chimeric birds by incubating fertilized bird eggs and injecting a retrovirus vector into these fertilized eggs excluding immediately after the initiation of the incubation. Namely, G0 transgenic chimeric birds having an antibody gene transferred thereinto by using a retrovirus vector with a lack of replicability and producing the antibody by expressing the thus transferred gene.
摘要翻译：要解决的问题：提供产生抗体的G0转基因嵌合鸟。解决方案：提供具有抗体基因的G0转基因嵌合鸟，其通过使用具有不可复制性的逆转录病毒载体转移到其中，并通过表达产生抗体转移基因; 通过收集由构建的G0转基因嵌合鸟表达的抗体来产生抗体的方法; 以及通过孵育受精鸟卵并将逆转录病毒载体注射到孵育开始后立即排除的这些受精卵中来构建G0转基因嵌合鸟的方法。即，通过使用具有不可复制性的逆转录病毒载体转移有抗体基因的G0转基因嵌合鸟，通过表达由此转移的基因产生抗体。

3. 发明专利

JP2008220224A Production of albumen-specific substance by ovalbumin promoter 有权
标题翻译：通过OVALBUMIN PROMOTER生产ALBUMEN特异性物质
公开(公告)号：JP2008220224A
公开(公告)日：2008-09-25
申请号：JP2007061038
申请日：2007-03-09
申请人： Kaneka Corp , Univ Nagoya , 国立大学法人名古屋大学 , 株式会社カネカ
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI
IPC分类号： C12N15/09 , A01K67/027
摘要： PROBLEM TO BE SOLVED: To provide a method for the oviduct-specific expression and the production of a protein at a practical level by using a promoter sequence functioning specifically to an avian oviduct as a method for producing a highly functional protein in a common egg, and solving the problem in a method for producing a highly functional protein for medicinal use by using a production technique with an egg of a transgenic hen, causing expression death in a systemic expression promoter which gives much expression quantity and may develop toxicity to the host in some objective proteins.
SOLUTION: The production of objective foreign protein in high productivity in an avian oviduct cell is achieved by realizing the oviduct-specific expression using an ovalbumin promoter and increasing its transcriptional activity. A transgenic hen exhibiting high expression in the oviduct can be prepared by cutting an ovalbumin promoter sequence to obtain the shortest possible sequence enabling the oviduct-specific expression, integrating VP16 factor derived from a tetracycline-responding herpes virus as a transcription activation factor to prepare a retrovirus vector increasing the transcriptional activity of the ovalbumin promoter, and preparing a transgenic hen.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：通过使用特异性地作为禽类输卵管起作用的启动子序列来提供在实际水平中输卵管特异性表达和生产蛋白质的方法，作为生产高功能性蛋白质的方法普通蛋，并且通过使用具有转基因母鸡的卵的生产技术来生产用于药物用途的高功能性蛋白质的方法中的问题，在全身表达启动子中引起表达死亡，其产生大量表达量并可能产生毒性宿主在一些客观的蛋白质。解决方案：通过使用卵清蛋白启动子实现输卵管特异性表达并增加其转录活性，实现禽输卵管细胞中高生产率的客观外源蛋白的产生。可以通过切割卵白蛋白启动子序列来获得能够产生输卵管特异性表达的最短序列，将来自四环素响应的疱疹病毒的VP16因子整合为转录激活因子来制备逆转录病毒载体增加卵清蛋白启动子的转录活性，并制备转基因母鸡。版权所有（C）2008，JPO＆INPIT

4. 发明专利

JP2007295846A Efficient production method of gene recombination whole antigen using internal ribosome binding site 有权
标题翻译：使用内部RIBOSOME绑定站点的全基因组重组的有效生产方法
公开(公告)号：JP2007295846A
公开(公告)日：2007-11-15
申请号：JP2006126673
申请日：2006-04-28
申请人： Japan Science & Technology Agency , Kaneka Corp , Univ Nagoya , 国立大学法人名古屋大学 , 株式会社カネカ , 独立行政法人科学技術振興機構
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI , KYOGOKU KENJI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/02 , C12P21/08
摘要： PROBLEM TO BE SOLVED: To increase production of immunoglobulin molecules in immunoglobulin production using animal culture cells, by well balanced production of two kinds of polypeptide, i.e., heavy chain and light chain of immunoglobulin molecules.
SOLUTION: A recombinant vector is provided by being arranged, in downstream of a promotor, a base sequence (A) encoding the heavy chain composing the immunoglobulin molecule and a base sequence (B) encoding the light chain composing the immunoglobulin molecule, and, between the base sequence (A) and the base sequence (B), an internal ribosome binding sequence derived from mRNA encoding homeodomain protein Gtx.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：通过动物培养细胞的免疫球蛋白生产中免疫球蛋白分子的产生，通过两种多肽（即免疫球蛋白分子的重链和轻链）的均衡生产来增加生产。解决方案：通过在启动子的下游设置编码构成免疫球蛋白分子的重链的碱基序列（A）和编码构成免疫球蛋白分子的轻链的碱基序列（B），提供重组载体，在碱基序列（A）和碱基序列（B）之间，衍生自编码同源结构域蛋白Gtx的mRNA的内部核糖体结合序列。版权所有（C）2008，JPO＆INPIT

5. 发明专利

JP2006271266A Highly expressing and efficient transgenic birds 审中-公开
标题翻译：高效表达和有效的转基因生物
公开(公告)号：JP2006271266A
公开(公告)日：2006-10-12
申请号：JP2005095758
申请日：2005-03-29
申请人： Kaneka Corp , Univ Nagoya , 国立大学法人名古屋大学 , 株式会社カネカ
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI
IPC分类号： A01K67/027 , C07K1/14 , C07K14/61 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide higher efficiency of expression of a target protein in transgenic birds.
SOLUTION: An autonomous replication ability-deficient type retrovirus is produced in a packaging cell which is established by using a retrovirus vector comprising a gene encoding the target protein and a post-transcriptional regulatory factor stabilizing an mRNA and integrated therein. An early embryo of a bird fertilized egg during a period of 24 h after starting incubation to 120 h is infected with the resultant retrovirus. G0 transgenic chimeric birds or offsprings thereof obtained by hatching and growing the embryo are provided.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供转基因鸟类中更高的靶蛋白表达效率。解决方案：在包装细胞中产生自主复制能力型逆转录病毒，其通过使用包含编码靶蛋白的基因的逆转录病毒载体和稳定mRNA并整合在其中的转录后调控因子来建立。在孵育至120小时后的24小时内，鸟受精卵的早期胚胎被所得到的逆转录病毒感染。提供通过孵化和生长胚胎获得的G0转基因嵌合鸟或其后代。版权所有（C）2007，JPO＆INPIT

6. 发明专利

JP2007275004A Method for producing gene recombinant birds by infection of lentivirus vector into spermary 审中-公开
标题翻译：通过感染淋巴细胞向胚胎生产基因重组体的方法
公开(公告)号：JP2007275004A
公开(公告)日：2007-10-25
申请号：JP2006107812
申请日：2006-04-10
申请人： Japan Science & Technology Agency , Kaneka Corp , Univ Nagoya , 国立大学法人名古屋大学 , 株式会社カネカ , 独立行政法人科学技術振興機構
发明人： IIJIMA SHINJI , KAMIHIRA MASAMICHI , NISHIJIMA KENICHI , KYOGOKU KENJI
IPC分类号： A01K67/027 , C12N5/10 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To need the high production of a transduced gene in G1 transgenic birds uniformly having transduced genes in all cells and in their progenies for practical uses, while a method for highly producing a target protein in G0 transgenic birds has been disclosed. SOLUTION: This method for producing the transgenic birds is characterized by transducing foreign genes into the male genitals of birds in their young chick period to insert the foreign genes into the genomes of male germ cells and/or supporting cells in a male genital. COPYRIGHT: (C)2008,JPO&INPIT

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