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1. 发明专利

JP2004298106A Method for separating d-amylose by crystallization method, and application thereof to mass-production 有权
标题翻译：通过结晶方法分离D-甲基的方法及其对大量生产的应用
公开(公告)号：JP2004298106A
公开(公告)日：2004-10-28
申请号：JP2003095828
申请日：2003-03-31
申请人： Kagawa Univ , 香川大学長
发明人： IKUMORI TAKESHI , TAKADA GORO , TOKUDA MASAAKI
IPC分类号： C12P19/02
摘要： PROBLEM TO BE SOLVED: To efficiently separate and collect D-amylose; and to provide a technically workable continuous production method concerning the production of the high-purity D-amylose.
SOLUTION: The method for separating and collecting the high-purity D-amylose comprises crystallizing the D-amylose by utilizing the property of slight solubility of the D-amylose in alcohol, and separating the crystal when collecting the D-amylose from an enzymic reaction product obtained by converting a part of a substrate convertible to the D-amylose to the D-amylose. The enzymic reaction product is obtained by passing a fed solution containing the substrate convertible to the D-amylose through a D-amylose-producing immobilized enzyme and/or a bioreactor using immobilized microorganisms. Various kinds of buffers, and the like, usually contained in the enzymic reaction product are not required to be removed when subjecting the reaction product to the action of the alcohol. The substrate convertible to the D-amylose is D-psicose, and the enzymic reaction product is a mixed solution of the D-psicose and the D-amylose. The mixed solution contains 35% D-psicose and 15% D-amylose.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：有效分离和收集D-直链淀粉; 并提供关于生产高纯度D-直链淀粉的技术上可行的连续生产方法。解决方案：用于分离和收集高纯度D-直链淀粉的方法包括通过利用D-直链淀粉在醇中的轻度溶解性的特性使D-直链淀粉结晶，并且当收集直链淀粉从D- 通过将可转换为D-直链淀粉的底物的一部分转化为D-直链淀粉而获得的酶反应产物。酶反应产物通过使用固定的微生物将含有可转化的底物的进料溶液通过产生D-直链淀粉的固定化酶和/或生物反应器送入D-直链淀粉而获得。通常在酶反应产物中含有的各种缓冲液等在使反应产物进行醇的作用时不需要除去。可转化为D-直链淀粉的底物是D-木糖，酶反应产物是D-木糖和D-直链淀粉的混合溶液。混合溶液含有35％的D-psicose和15％的D-直链淀粉。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004298105A Method for immobilizing l-rhamnose isomerase 审中-公开
标题翻译：用于固定L-RHAMNOSE异构体的方法
公开(公告)号：JP2004298105A
公开(公告)日：2004-10-28
申请号：JP2003095827
申请日：2003-03-31
申请人： Kagawa Univ , 香川大学長
发明人： IKUMORI TAKESHI , TAKADA GORO , TOKUDA MASAAKI
IPC分类号： C12N11/00 , C12N11/16 , C12P19/02
摘要： PROBLEM TO BE SOLVED: To provide an immobilized L-rhamnose isomerase having high activities and stable enzymic activities, and allowing a rapid flow rate when used for a bioreactor.
SOLUTION: The method for immobilizing the L-rhamnose isomerase comprises adding glutaraldehyde to the L-rhamnose isomerase after precipitating, or purifying and crystallizing the L-rhamnose isomerase when an extract from a biomass is used, or as it its when the biomass itself is used to cause crosslinking by a covalent bond, and adding lysine thereto to enhance the strength by increasing the crosslinking density. The immobilized L-rhamnose isomerase allowing a solution-sending pressure to be reduced when the bioreactor is constituted, and having a stable shape enabling long-term continuous use is obtained by the method. The use of the immobilized L-rhamnose isomerase for a method for continuously producing D-amylose is also provided.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供具有高活性和稳定的酶活性的固定的L-鼠李糖异构酶，并且当用于生物反应器时允许快速流速。解决方案：用于固定L-鼠李糖异构酶的方法包括在使用生物质提取物之后，在沉淀后，向L-鼠李糖异构酶中加入戊二醛，或者当使用来自生物质的提取物时纯化和结晶L-鼠李糖异构酶，生物质本身用于通过共价键引起交联，并且通过增加交联密度将赖氨酸加入其中以增强强度。通过该方法可获得固定化的L-鼠李糖异构酶，其能够在构成生物反应器时使溶液发送压力降低，并且具有能够长期连续使用的稳定形状。还提供了固定的L-鼠李糖异构酶用于连续生产D-直链淀粉的方法。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2005102503A Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof 审中-公开
标题翻译：具有新催化功能的L-赖氨酸异构酶的基因序列及其用途
公开(公告)号：JP2005102503A
公开(公告)日：2005-04-21
申请号：JP2003299371
申请日：2003-08-22
申请人： Kagawa Univ , 国立大学法人香川大学
发明人： IKUMORI TAKESHI , TAKADA GORO , TOKUDA MASAAKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/90 , C12P19/02 , C12P19/24
CPC分类号： C12N9/90 , C12P19/02 , C12P19/24 , C12Y503/01014
摘要： PROBLEM TO BE SOLVED: To establish a reaction system for producing a plurality of kinds of rare sugars by reacting a plurality of kinds of rare aldoses and obtaining the most efficient isomerase for producing a plurality of kinds of rare ketoses in the strategy of the rare sugars of Izumoring (shown in the figure). SOLUTION: A DNA encodes the following protein (a) or (b). (a) A protein composed of an amino acid sequence represented by sequence number 2 or (b) a protein composed of an amino acid sequence in which one or several amino acids are deleted, substituted or added in the amino acid sequence represented by the sequence number 2 and having an L-rhamnose isomerase activity. The DNA is the L-rhamnose isomerase derived from Pseudomonas stutzerii. The protein is composed of the amino acid sequence represented by the sequence number 2. A method for producing a recombinant protein comprises culturing a host cell containing an expression system expressing the protein in a culture medium and collecting the recombinant protein having the L-rhamnose isomerase activity from the resultant cultured product. A method is to utilize the figure for producing the rare sugars by understanding the positions of the objective rare sugars in monosaccharides in the whole image and design the optimum production route thereof for reacting the protein. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：通过使多种稀有醛糖反应制备多种稀有糖的反应体系，获得用于制备多种稀有酮糖的最有效的异构酶，其策略为喷出的稀有糖（如图所示）。解决方案：DNA编码以下蛋白质（a）或（b）。（a）由序列号2所示的氨基酸序列构成的蛋白质，或者（b）由氨基酸序列组成的蛋白质，其中一个或多个氨基酸缺失，取代或添加在序列表示的氨基酸序列中 2号并具有L-鼠李糖异构酶活性。 DNA是源自番茄假单胞菌的L-鼠李糖异构酶。蛋白质由序列号2表示的氨基酸序列组成。重组蛋白质的制造方法包括在培养基中培养含有表达该蛋白质的表达系统的宿主细胞，收集具有L-鼠李糖异构酶的重组蛋白质所得培养产物的活性。一种方法是通过了解整个图像中单糖中目标稀有糖的位置来制备稀有糖，并设计其用于使蛋白质反应的最佳生产途径。版权所有（C）2005，JPO＆NCIPI

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