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1. 发明专利

JPH1156355A PRODUCTION OF CHOLESTEROL ESTERASE 失效
公开(公告)号：JPH1156355A
公开(公告)日：1999-03-02
申请号：JP23916397
申请日：1997-08-21
申请人： KIKKOMAN CORP
发明人： HIRUMA MINORU , SHIROKANE TAKAO
IPC分类号： C12N9/16 , C12N1/20 , C12R1/64
摘要： PROBLEM TO BE SOLVED: To produce the subject enzyme having the optimum pH or a slightly acidic side excellent in thermal stability and useful for determination or the like of an ester-type cholesterol by culturing cholesterol esterase-producing microorganisms belonging to the genus Xanthomonas. SOLUTION: A strain [e.g. Xanthomonas sp. No. 81-13 (FERM P-16369)] belonging to the genus Xanthomonas and having cholesterol esterase-producing activities is cultured in a medium, and the objective cholesterol esterase is collected in the method for producing the cholesterol esterase. The cholesterol esterase derived from the Xanthomonas sp. No. 81-13 (1) strongly acts on cholesterol oleate, cholesterol linolate, and cholesterol palmitate in order, and further slightly acts on cholesterol acetate, (2) has the optimum pH at nearly pH 6. 5, and a stable pH region within 5.5-8. 5, (3) has the acting optimum temperature within a rage of 45-50 deg.C, and (4) has about 55,000 molecular weight (SDS-PAGE).

2. 发明专利

JPH08168398A REAGENT FOR DETERMINING MALTOSE AND METHOD FOR DETERMINING THE SAME 失效
公开(公告)号：JPH08168398A
公开(公告)日：1996-07-02
申请号：JP33382894
申请日：1994-12-19
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , SUZUKI MASARU
IPC分类号： C12Q1/533
摘要： PURPOSE: To obtain the reagent for a sensory test and a diabetes test, containing a maltose 1-epimerase for catalyzing dismutation reaction of β-maltose and α-maltose. CONSTITUTION: This determining reagent has catalyzing action on dismutation reaction between β-maltose and α-maltose, does not act on cellobiose and lactose, has optimum pH5.5 to pH7.5, a stable pH range of pH5.0 to pH8.0 at 30 deg.C for 30 minutes and about 43,000 molecular weight (gel filtration method), is obtained by combining a maltose 1-epimerase with maltose phosphorylase, phosphate ion or arsenate ion and a reagent for measuring a formed product, makes it possible to smoothly carry out enzyme reaction of β-maltose with maltose phosphorylase hardly to act on β-maltose by adding maltose-1-epimerase and is capable of determining maltose in a food and blood in a short time and accurately.

3. 发明专利

JPH07155181A NEW MALTOBIONIC ACID GLUCOHYDRASE, ITS PRODUCTION AND REAGENT FOR DETERMINING MALTOBIONIC ACID AND METHOD FOR DETERMINATION USING THE SAME ENZYME 失效
公开(公告)号：JPH07155181A
公开(公告)日：1995-06-20
申请号：JP33914493
申请日：1993-12-06
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , ARAI AYUMI , UCHIDA RIICHIRO , NASU AYAKO , YAMATSUGU NOBUYUKI
IPC分类号： C12N9/26 , C12Q1/34 , C12R1/07
摘要： PURPOSE:To obtain a new enzyme, capable of acting on maltobionic acid without acting on maltose or maltitol and useful for determining, etc., the maltobionic acid by culturing a strain, which belongs to the genus Bacillus and capable of producing maltobionic acid glucohydrase. CONSTITUTION:This new maltobionic acid glucohydrase is obtained by inoculating a strain, which belongs to the genus Bacillus and capable of producing the maltobionic acid glucohydrase [e.g. Bacillus sp. N-1053 (FERM P-13975)] into a culture medium, culturing the strain at 30 deg.C for about 24hr, then centrifuging the culture solution, collecting the microbial cell, and collecting the resultant product from the microbial cell. The obtained maltobionic acid glucohydrase is capable of hydrolyzing the maltobionic acid, producing beta-D-glucose and D- gluconic acid without acting on maltose or maltitol, having 6.5-8.0 optimum pH, pH5.5-9.5 stable pH range by treatment at 30 deg.C for 30min and about 150000 molecular weight (a gel filtration method). The maltobionic acid glucohydrase is capable of readily determining the maltobionic acid with a good accuracy according to simple operations.

4. 发明专利

JPS6274285A STABILIZATION OF MONOMETHYLAMINE OXIDASE 失效
公开(公告)号：JPS6274285A
公开(公告)日：1987-04-06
申请号：JP21364885
申请日：1985-09-28
申请人： KIKKOMAN CORP
发明人： NAKAMURA KAZUO , NAKAJIMA MOTOO , SHIROKANE TAKAO
IPC分类号： C12N9/96
摘要： PURPOSE:To prevent inactivation of an enzyme and improve the stability during preservation for a long period, by adding a specific compound to a solution containing monomethylamine oxidase. CONSTITUTION:One or more of about 0.1-10wt/vol% saccharide, about 1-30mM heavy metal salt, about 1-30mM alkali metal salt, about 1-30mM alkaline earth metal salt, about 0.1-10wt/vol% amino acid, about 0.1-10wt/vol% organic acid salt, about 0.1-10wt/vol% guanidino compound and about 0.1-10mM chelating agent respectively alone or in combination are added and dissolved in a solution containing monomethylamine oxidase.

5. 发明专利

JPS60203189A PREPARATION OF ENZYME DECOMPOSING GUANIDINOACETIC ACID 失效
公开(公告)号：JPS60203189A
公开(公告)日：1985-10-14
申请号：JP5738284
申请日：1984-03-27
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , NAKAJIMA MOTOO
IPC分类号： C12N9/78 , C12N9/80 , C12R1/06 , C12R1/15
摘要： PURPOSE:To obtain efficiently the titled enzyme for quantitative determination of guanidinoacetic acid for diagnosis of renal disorder, by cultivating a strain, belonging to the genus Corynebacterium, etc., and having the ability to produce an enzyme decomposing guanidinoacetic acid in a culture medium, and separating and purifying the resultant product from the culture. CONSTITUTION:A strain, e.g. Corynebacterium sp. N-19-1 (FERM-P No.506), belonging to the genus Corynebacterium or Arthrobacter, and having the ability to produce an enzyme capable of decomposing guanidinoacetic acid is inoculated into a culture medium and cultivated, and the culture medium and cultivated, and the culture fluid is centrifuged to collect microbial cells, which are suspended in a buffer solution at 8.0pH. Cells are disintegrated while being cooled in an ultrasonic disintegrator, etc., and precipitates are removed by centrifugation to concentrate the supernatant liquid and give the aimed enzyme.

6. 发明专利

JP2010281652A Peroxidase chemiluminescence measuring reagent 有权
标题翻译：过氧化物酶联免疫吸附试剂
公开(公告)号：JP2010281652A
公开(公告)日：2010-12-16
申请号：JP2009134576
申请日：2009-06-04
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： SHIROKANE TAKAO , KAWAGUCHI SEI
IPC分类号： G01N21/77 , C12Q1/28
摘要： PROBLEM TO BE SOLVED: To provide a chemiluminescence measuring reagent of a basidiomycete-derived peroxidase (POD), which is not inferior to that of a horseradish POD in performance, and maintains an initial luminescence intensity superior to that of same. SOLUTION: A chemiluminescence measurement of the basidiomycete-derived POD is carried out by using the reagent, such that three factors of a content of an oxidizing agent, a content of a chemiluminescence substance and a pH value of a buffer solution are chosen and combined appropriately. In this method using the chemiluminescence measuring reagent of the basidiomycete-derived POD, which is not inferior but superior to that of the horseradish POD in performance, a rate of 70% or above of the initial luminescence intensity is maintained for the elapsed time of ten minutes after starting a chemiluminescence reaction. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供在性能上不逊于辣根POD的担子菌来源的过氧化物酶（POD）的化学发光测定试剂，并且保持初始发光强度优于其它的发光强度。解决方案：通过使用试剂进行担子菌素衍生的POD的化学发光测量，从而选择氧化剂含量，化学发光物质的含量和缓冲溶液的pH值的三个因素并合理组合。在该方法中，使用化学发光测定试剂的担子菌素衍生的POD，其性能不逊色，但优于辣根POD，其初始发光强度的70％以上的速率保持为10 开始化学发光反应后几分钟。版权所有（C）2011，JPO＆INPIT

7. 发明专利

JPH08242855A PRODUCTION OF NEW MALTOSE 1-EPIMERASE 失效
公开(公告)号：JPH08242855A
公开(公告)日：1996-09-24
申请号：JP7243995
申请日：1995-03-07
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , SUZUKI MASARU
IPC分类号： C12N9/90 , C12R1/01 , C12R1/46
摘要： PURPOSE: To easily produce a new maltose 1-epimerase having a specific reactivity with maltose and capable of determining the maltose in a short time by culturing a specific strain having a maltose 1-epimerase-producing activity in a medium. CONSTITUTION: This maltose 1-epimerase is obtained by culturing a strain (Streptococcus, Leuconostoc, Entericoccus or Pediococcus) having a maltose 1-epimerase-producing activity in a medium and recovering the epimerase from the cultured mixture. This maltose 1-epimerase exhibits a catalytic activity for an interconversion reaction between β-maltose and α-maltose, specifically reacts with maltose substantially without reacting with cellobiose and lactose and has an optimum pH in 5.5-7.5 and a molecular weight of about 43000 (gel filtration method).

8. 发明专利

JPS6087800A METHOD AND REAGENT FOR DETERMINATION OF GUANIDINO COMPOUND 失效
公开(公告)号：JPS6087800A
公开(公告)日：1985-05-17
申请号：JP19351883
申请日：1983-10-18
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , NAKAJIMA MOTOO
IPC分类号： G01N33/70 , C12Q1/58
摘要： PURPOSE:To determine the guanidino compound in urine or blood, rapidly, accurately and effectively, by using an immobilized urease in combination with a guanidinoacetic acid lytic enzyme or methyl-guanidine lytic enzyme. CONSTITUTION:Urea in a specimen causing the determination error is decomposed by treating urine or blood with an immobilized urease, and the obtained specimen liquid is made to react with a guanidinoacetic acid lytic enzyme or a methylguanidine lytic enzyme to produce urea. The produced urea is determined by the use of a conventional color-development reagent for urea or a reagent to produce a fluorescent material by the reaction with urea.

9. 发明专利

JPH08280382A NEW MALTOSE PHOSPHORYLASE AND ITS PRODUCTION 失效
公开(公告)号：JPH08280382A
公开(公告)日：1996-10-29
申请号：JP11270695
申请日：1995-04-14
申请人： KIKKOMAN CORP
发明人： HIRUMA MINORU , SHIROKANE TAKAO , SUZUKI MASARU
IPC分类号： C12N9/10 , C12R1/01
摘要： PURPOSE: To obtain the subject new enzyme, useful for measurement, etc., of the maltose concentration in a food and having high thermostability by culturing a microorganism, belonging to the genus Enterococcus, Leuconostoc, Lactococcus, etc., and capable of producing a maltose phosphorylase. CONSTITUTION: A strain, belonging to the genus Enterococcus, Leuconostoc or Lactococcus and having the ability to produce a maltose phosphorylase (e.g. Enterococcus hirae IFO3181) is cultured in a culture medium and a product is collected from its cultured product to afford a new maltose phosphorylase, capable of carrying out the phosphorolysis of maltose and producing β-D-glucose 1-phosphate and D-glucose without acting on sucrose, lactose, trehalose, maltitol and cellobiose, having optimal pH at 6.5-7.5, stable at a pH of 5.5-8.0, having 45-50 deg.C optimal action temperature and strongly inhibited with HgCl2 and AgNO3 . The maltose phosphorylase is useful for determination, etc., of the maltose content in a food.

10. 发明专利

JPH0491797A REAGENT FOR DETERMINATION OF GUANIDINOACETIC ACID AND DETERMINATION METHOD THEREOF 失效
公开(公告)号：JPH0491797A
公开(公告)日：1992-03-25
申请号：JP20762190
申请日：1990-08-07
申请人： KIKKOMAN CORP
发明人： SHIROKANE TAKAO , NAKAJIMA MOTOO , MIZUSAWA KIYOSHI , MARUI YOJI , HAYASHI CHOZO
IPC分类号： C12Q1/25 , C12Q1/34
摘要： PURPOSE:To quickly and accurately determine guanidinoacetic acid in a guanidinoacetic acid-containing sample in extremely simple operation by adopting a determination method in which a specific enzyme is combined with a substrate. CONSTITUTION:A guanidinoacetic acid-containing sample is reacted with at least one kind selected from a group consisting of guanidinoacetic acid decompos ing enzyme, glutathione synthetic enzyme, gamma-glutamyl derivative, aminolactic acid derivative and cystine derivative, at least one kind selected from a group of adenosine-5'-triphosphate and salt thereof and metal ion. Then, guanidinoacetic acid in the sample is quantitatively analyzed by measuring an amount of the resulting adenosine-5'-diphosphate, ortho-phosphoric acid or a glycine derivative. In the above-mentioned determination, it is desired to control pH of these samples to about pH 4-12 by a proper pH controller such as hydrochloric acid, sulfuric acid, nitric acid, sodium hydroxide or potassi um hydroxide, though these sample may be used without control.

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