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    • 2. 发明专利
    • Method for producing raw material for enzymatic saccharification, method for producing sugar, and method for producing ethanol
    • 用于生产食用原料的方法,用于生产糖的方法和用于生产乙醇的方法
    • JP2012143179A
    • 2012-08-02
    • JP2011003292
    • 2011-01-11
    • Jx Nippon Oil & Energy CorpJx日鉱日石エネルギー株式会社Univ Of Tokyo国立大学法人 東京大学
    • SAMEJIMA MASAHIROIGARASHI KIYOHIKOWADA MASAHISAIGUCHI YASUTOSHIISOMURA YOSHIISANIWA MASAHIRO
    • C13K1/02C12P7/08C12P19/02
    • C13K1/02Y02E50/16
    • PROBLEM TO BE SOLVED: To provide a method for producing raw materials for enzymatic saccharification by treating lignocellulose-containing plant biomass with ammonia, capable of obtaining raw materials for enzymatic saccharification with a high enzymatic saccharification efficiency, and capable of reducing the energy required for separating and collecting ammonia.SOLUTION: The method is characterized by the amount of water present within a system for the treatment satisfying the following formula (1), in which the mass of water/(the dry mass of biomass+the mass of water) ≤0.30 (1); and by the temperature and pressure of ammonia in the treatment which are located within a closed region formed by a saturated vapor pressure line, a curve obtained by moving the saturated steam pressure line parallel in the direction of the ordinate axis by -1.5 kmol/m, a straight line orthogonally crossing the abscissa axis at a point of the temperature of 60°C and the density of 0 kmol/m, and an isobar of the pressure of 1.7 MPaA in an ammonia phase diagram wherein pressure is plotted with isobars and wherein the abscissa axis is a temperature axis and the ordinate axis is a density axis.
    • 待解决的问题:提供一种通过用氨处理含木素纤维素的植物生物质来生产用于酶糖化的原料的方法,其能够以高酶促糖化效率获得酶糖化的原料,并且能够降低能量 分离和收集氨需要。 解决方案:该方法的特征在于满足下列公式(1)的处理系统内存在的水量,其中水的质量(生物质的干重+水的质量)≤0.30 (1); 并且通过位于由饱和蒸汽压力线形成的闭合区域内的处理中的氨的温度和压力,将沿纵轴方向平行的饱和蒸汽压力线移动-1.5kmol / m而获得的曲线 3 ,在温度为60℃的点处垂直于横轴的直线和0kmol / m的密度 3 < / SP>,和在氨相图中压力为1.7MPaA的等压线,其中压力用等压线绘制,其中横坐标轴是温度轴,纵坐标轴是密度轴。 版权所有(C)2012,JPO&INPIT
    • 3. 发明专利
    • Method for producing sugar, method for producing ethanol, method for producing lactic acid, cellulose for enzyme saccharification used for them and method for producing the same
    • 用于生产糖的方法,用于生产乙醇的方法,用于生产柠檬酸的方法,用于本发明的用于酶解的纤维素及其生产方法
    • JP2008161125A
    • 2008-07-17
    • JP2006354706
    • 2006-12-28
    • Univ Of Tokyo国立大学法人 東京大学
    • WADA MASAHISAIGARASHI KIYOHIKOSAMEJIMA MASAHIRO
    • C12P19/14C08B1/00C12P7/10C12P7/56C12P19/02
    • Y02E50/16
    • PROBLEM TO BE SOLVED: To provide a method for producing a sugar, method for producing ethanol and method for producing lactic acid, capable of performing an enzyme-saccharification efficiently and therefore capable of improving the production efficiency of the sugar, production efficiency of the ethanol and the production efficiency of the lactic acid, and the cellulose for enzyme saccharification used for each of the above production methods and method for producing the same. SOLUTION: This method for producing the sugar by enzymatically saccharifying cellulose having a lower crystal density than that of the type I cellulose; the method for producing the ethanol by fermenting the sugar obtained in the previous method for producing the sugar and the method for obtaining the lactic acid for obtaining the lactic acid; the cellulose used for enzymatic saccharification, having the lower crystal density than that of the type I cellulose and used for the above methods of production; and the method for producing the cellulose used for the enzymatic saccharification comprises the treatment of biomass raw materials containing the type I cellulose by a compound having ≥1 amino group (NH 2 ) or ammonia molecule (NH 3 ) are provided. COPYRIGHT: (C)2008,JPO&INPIT
    • 待解决的问题:为了提供糖的制造方法,制造乙醇的方法及其制造方法,能够高效地进行糖化,能够提高糖的生产效率,生产效率 的乙醇和生产效率的乳酸,以及用于上述制备方法和制备方法的酶糖化用纤维素。 解决方案:通过酶法糖化具有比I型纤维素低的结晶密度的纤维素来生产糖的方法; 通过发酵上述制造糖的方法得到的糖来制造乙醇的方法以及获得乳酸的乳酸的制造方法, 用于酶糖化的纤维素,其结晶密度低于I型纤维素,并用于上述生产方法; 制造用于酶促糖化的纤维素的方法包括通过具有≥1个氨基的化合物(NH 2 )或氨分子(NH 3 )。 版权所有(C)2008,JPO&INPIT
    • 6. 发明专利
    • New protein having endoglucanase activity, its dna and use thereof
    • 具有内切酶活性的新蛋白,其DNA及其用途
    • JP2010022242A
    • 2010-02-04
    • JP2008185219
    • 2008-07-16
    • Univ Of Tokyo国立大学法人 東京大学
    • IGARASHI KIYOHIKOSAMEJIMA MASAHIRO
    • C12N15/09A23K1/16A23L1/29C11D3/386C12N1/15C12N1/19C12N1/21C12N5/10C12N9/42C12P7/10C12P19/02
    • C12N9/2437A23K20/147A23L33/195C11D3/38645C12Y302/01004Y02E50/16
    • PROBLEM TO BE SOLVED: To find a new protein that is easily usable with respect to safety, etc., obtained from a basidiomycete into which an edible mushroom is classified and has endoglucanase activity; and to provide a new DNA encoding the protein; a vector having the DNA; a transformant having the vector; a method for producing a protein having endoglucanase activity, which uses the transformant; a method for producing a saccharide, which uses at least one of a protein having endoglucanase activity and a basidiomycete; a method for producing ethanol using the saccharide obtained by the method for producing the saccharide; a food containing a protein having endoglucanase activity, a feed, a detergent; and a method for treating a cellulose-containing organic substance using a protein having endoglucanase activity.
      SOLUTION: The protein is a basidiomycete-derived protein and having a molecular weight measured by SDS-PAGE of 18 kDa and endoglucanase activity.
      COPYRIGHT: (C)2010,JPO&INPIT
    • 要解决的问题:从可食用蘑菇被分类并具有内切葡聚糖酶活性的担子菌获得的关于安全性等容易使用的新蛋白质; 并提供编码蛋白质的新DNA; 具有DNA的载体; 具有载体的转化体 使用该转化体的生产具有内切葡聚糖酶活性的蛋白质的方法; 使用至少一种具有内切葡聚糖酶活性的蛋白质和担子菌的糖的生产方法; 使用通过该糖的制造方法得到的糖制造乙醇的方法; 含有具有内切葡聚糖酶活性的蛋白质的食物,饲料,洗涤剂; 以及使用具有内切葡聚糖酶活性的蛋白质处理含纤维素的有机物质的方法。 解决方案:蛋白质是担子菌素衍生的蛋白质,其分子量通过SDS-PAGE测量为18kDa和内切葡聚糖酶活性。 版权所有(C)2010,JPO&INPIT
    • 8. 发明专利
    • Method for detecting biological sample
    • 检测生物样品的方法
    • JP2007236215A
    • 2007-09-20
    • JP2006059113
    • 2006-03-06
    • Univ Of Tokyo国立大学法人 東京大学
    • IGARASHI KIYOHIKOSAMEJIMA MASAHIRO
    • C12Q1/68C12N15/09G01N33/15
    • PROBLEM TO BE SOLVED: To provide a technique for simply detecting a specimen, in which it is feared that contain a biological sample in slight amount, in a short time and high sensitivity. SOLUTION: The method for detecting the biological sample comprises (1) a step for non-specifically amplifying a genome DNA in the biological sample contained in the specimen in the presence of DNA polymerase and random hexamer enabling a MDA (Multiple Displacement Amplification) method and (2) a step for carrying out PCR analysis by using the resultant amplified product as a template. The detection method is suitably used for detecting or monitoring a wood-decaying fungus, a sanitary insect pest, a fungus, a stored grain insect pest each contained in a minute amount in housing construction materials, bedding, grains, soil, fruit trees, etc., in high sensitivity. COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供一种简单地检测在短时间和高灵敏度下容易含有少量生物样品的样本的技术。 解决方案:用于检测生物样品的方法包括(1)在DNA聚合酶和随机六聚体存在下,在样品中包含的生物样品中非特异性扩增基因组DNA的步骤,其使得能够进行MDA(多重位移扩增 )方法和(2)通过使用得到的扩增产物作为模板进行PCR分析的步骤。 该检测方法适用于检测或监测住房建筑材料,床上用品,谷物,土壤,果树等中微量数量的木材腐烂真菌,卫生虫害,真菌,储存的害虫。 灵敏度高。 版权所有(C)2007,JPO&INPIT