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1. 发明专利

JP2013150620A Glucose dehydrogenase 有权
标题翻译：葡萄糖脱氢酶
公开(公告)号：JP2013150620A
公开(公告)日：2013-08-08
申请号：JP2013048916
申请日：2013-03-12
申请人： Ikeda Shokken Kk , 池田食研株式会社
发明人： HONDA MICHINARI , TAKI SAYAKA , TAKENAKA RYO
IPC分类号： C12N9/04 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide an efficient method for producing a glucose dehydrogenase, specifying and isolating a glucose dehydrogenase gene originated from Botryotinia fuckelinana, and efficiently producing a recombined enzyme.SOLUTION: This flavin-bound form glucose dehydrogenase comprising a glycopolypeptide of following (e) or (f), obtained by culturing transformed eukalyotic cells prepared by using a recombined vector including a polynucleotide: (e) a glycopolypeptide comprising a specific amino acid sequence, and (f) a glycopolypeptide comprising an amino acid sequence having at least 90% identity to the specific amino acid sequence, also having glucose dehydrogenase activity and an N-terminal of STLNY.
摘要翻译：要解决的问题：提供一种生产葡萄糖脱氢酶的有效方法，指定和分离源自葡萄孢菌的葡萄糖脱氢酶基因，并有效地产生重组酶。解决方案：该黄素结合形式的葡萄糖脱氢酶包含以下的糖多肽（通过培养通过使用包含多核苷酸的重组载体制备的转化真核细胞获得的（e）或（f））：（e）包含特定氨基酸序列的糖多肽，和（f）包含具有至少90个氨基酸序列的氨基酸序列的糖多肽与具有葡萄糖脱氢酶活性的特定氨基酸序列的％同一性和STLNY的N-末端。

2. 发明专利

JP2012132926A Proteinous electron mediator 有权
标题翻译：蛋白电子介质
公开(公告)号：JP2012132926A
公开(公告)日：2012-07-12
申请号：JP2012022582
申请日：2012-02-04
申请人： Ikeda Shokken Kk , 池田食研株式会社
发明人： TAKENAKA RYO , HONDA MICHINARI , KOMURA KEIGO
IPC分类号： G01N27/327 , C12N9/04 , C12N15/09 , C12Q1/26 , G01N27/416
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： PROBLEM TO BE SOLVED: To provide an electron mediator and a fused substance having high affinity with an enzyme, and a measuring method, an electrode and a sensor using an extracellular secretion cytochrome and an enzyme.SOLUTION: The invention provides an electron mediator for glucose oxidoreductase including an extracellular secretion cytochrome; a fused substance obtained by fusing the electron mediator with glucose oxidoreductase; a composition for use in measuring glucose including the electron mediator or the fused substance; a gene for coding a novel extracellular secretion cytochrome; and a measuring method, an electrode and a sensor using an extracellular secretion cytochrome and an enzyme.
摘要翻译：要解决的问题：提供与酶具有高亲和力的电子介体和融合物质，以及使用细胞外分泌细胞色素和酶的测量方法，电极和传感器。解决方案：本发明提供了包括细胞外分泌细胞色素的葡萄糖氧化还原酶的电子介体; 通过将电子介体与葡萄糖氧化还原酶融合而得到的熔融物质; 用于测量葡萄糖的组合物，包括电子介体或熔融物质; 用于编码新型细胞外分泌细胞色素的基因; 以及使用细胞外分泌细胞色素和酶的测定方法，电极和传感器。版权所有（C）2012，JPO＆INPIT

3. 发明专利

JP2013090620A Glucose dehydrogenase 有权
标题翻译：葡萄糖脱氢酶
公开(公告)号：JP2013090620A
公开(公告)日：2013-05-16
申请号：JP2012136112
申请日：2012-06-15
申请人： Ikeda Shokken Kk , 池田食研株式会社
发明人： HONDA MICHINARI , TAKI SAYAKA , TAKENAKA RYO
IPC分类号： C12N15/09 , C12M1/34 , C12N1/15 , C12N1/19 , C12N5/10 , C12N9/04 , C12Q1/32 , C12Q1/54
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32
摘要： PROBLEM TO BE SOLVED: To provide an efficient method for producing a glucose dehydrogenase to specify and isolate a glucose dehydrogenase gene originating in Botrytis bassiana to efficiently produce a recombinant enzyme.SOLUTION: A biosensor for assaying glucose, characterized by using a flavin-bound glucose dehydrogenase that comprises glycopolypeptide (e) or (f): (e) a glycopolypeptide comprising an amino acid sequence represented by SEQ ID NO:5; and (f) a glycopolypeptide comprising an amino acid sequence that is derived from the amino acid sequence represented by SEQ ID NO:5 by substitution, deletion or addition of one to several amino acids and having glucose dehydrogenase activity.
摘要翻译：要解决的问题：提供一种生产葡萄糖脱氢酶以有效地制备葡萄糖脱氢酶基因的方法，以有效地产生重组酶。解决方案：用于测定葡萄糖的生物传感器，其特征在于使用包含糖多肽（e）或（f）的黄素结合的葡萄糖脱氢酶：（e）包含SEQ ID NO：5所示的氨基酸序列的糖多肽; 和（f）包含通过取代，缺失或添加1至数个氨基酸并具有葡萄糖脱氢酶活性而衍生自SEQ ID NO：5所示氨基酸序列的氨基酸序列的糖多肽。版权所有（C）2013，JPO＆INPIT

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