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1. 发明专利

JP2005110687A Tool and method for collecting nucleic acid 有权
标题翻译：用于收集核酸的工具和方法
公开(公告)号：JP2005110687A
公开(公告)日：2005-04-28
申请号：JP2004337944
申请日：2004-11-22
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SAKURAI TOMOYA , YOKOBAYASHI TOSHIAKI
IPC分类号： C12N15/09 , B01J20/281 , B01J20/283 , B01L3/02 , C12M1/00 , C12M1/26 , C12Q1/68 , G01N30/08 , G01N30/26 , G01N30/88 , G01N33/50 , G01N35/00 , G01N35/02 , G01N35/10 , G01N30/48
CPC分类号： G01N35/109 , B01F2215/0037 , B01L3/0275 , B01L2200/0631 , G01N35/0099 , G01N35/028 , G01N35/1004 , G01N2035/00237 , G01N2035/103 , G01N2035/1058 , Y10T436/255
摘要： PROBLEM TO BE SOLVED: To provide a tool and method for conveniently collecting nucleic acids from biological samples containing nucleic acids. SOLUTION: A chip for catching nucleic acids has a built-in silica-containing solid phase so as to contact with a liquid, wherein the solid phase has a water passage portion and an average gap between the solid phases in the water passage portion is not more than 25 μm. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于从含有核酸的生物样品中方便地收集核酸的工具和方法。解决方案：用于捕获核酸的芯片具有内置的含二氧化硅的固相以与液体接触，其中固相具有水通道部分和水通道中固相之间的平均间隙部分不超过25μm。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004298195A Method for recovering nucleic acid 有权
公开(公告)号：JP2004298195A
公开(公告)日：2004-10-28
申请号：JP2004203023
申请日：2004-07-09
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SAKURAI TOMOYA , YASUDA KENJI
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for collecting a nucleic acid, capable of quickly and readily collecting the nucleic acid having high purity without lowering yield from a material containing the nucleic acid.
SOLUTION: The method for collecting a nucleic acid comprises the first process of accelerating isolation of the nucleic acid from a material containing the nucleic acid, the second process of mixing the isolated nucleic acid with a substance accelerating bonding of the nucleic acid to a solid phase, the third process of bringing the resultant mixed solution into contact with a nucleic acid-bondable solid phase, the fourth process of separating the solid phase from the liquid, the fifth process of cleaning the solid phase bonded with the nucleic acid by using a solution containing a salt and the sixth process of eluting the nucleic acid from the solid phase. By the method, a nucleic acid having a purity suitable for a genetic screening or a genetic analysis can be quickly and readily collected without using any hazardous substance.
COPYRIGHT: (C)2005,JPO&NCIPI

3. 发明专利

JP2007330271A Method for recovering nucleic acid 有权
标题翻译：回收核酸的方法
公开(公告)号：JP2007330271A
公开(公告)日：2007-12-27
申请号：JP2007244920
申请日：2007-09-21
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SAKURAI TOMOYA , YASUDA KENJI
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for collecting a nucleic acid, capable of quickly and readily collecting the nucleic acid having high purity without lowering yield from a material containing the nucleic acid.
SOLUTION: The method for collecting a nucleic acid comprises the first process of accelerating isolation of the nucleic acid from a material containing the nucleic acid, the second process of mixing the isolated nucleic acid with a substance accelerating bonding of the nucleic acid to a solid phase, the third process of bringing the resultant mixed solution into contact with a nucleic acid-bondable solid phase, the fourth process of separating the solid phase from the liquid, the fifth process of cleaning the solid phase bonded with the nucleic acid by using a solution containing a salt and the sixth process of eluting the nucleic acid from the solid phase. By the method, a nucleic acid having a purity suitable for a genetic screening or a genetic analysis can be quickly and readily collected without using any hazardous materials.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供一种收集核酸的方法，其能够快速且容易地收集具有高纯度的核酸而不降低含有该核酸的材料的产率。解决方案：收集核酸的方法包括加速从含有核酸的材料中分离核酸的第一种方法，将分离的核酸与促进核酸结合的物质混合的第二种方法固相，将所得混合溶液与可与核酸结合的固相接触的第三种方法，从液体中分离固相的第四种方法，第五种用核酸清除固相的方法使用含有盐的溶液和从固相中洗脱核酸的第六种方法。通过该方法，可以快速且容易地收集具有适用于遗传筛选或遗传分析的纯度的核酸，而不使用任何有害物质。版权所有（C）2008，JPO＆INPIT

4. 发明专利

JP2004290211A Tool and method for collecting nucleic acid 有权
公开(公告)号：JP2004290211A
公开(公告)日：2004-10-21
申请号：JP2004215651
申请日：2004-07-23
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SAKURAI TOMOYA , YOKOBAYASHI TOSHIAKI
IPC分类号： C12N15/09 , B01J20/281 , B01J20/283 , B01L3/02 , C12M1/00 , C12M1/26 , C12Q1/68 , G01N30/08 , G01N30/26 , G01N30/88 , G01N33/50 , G01N35/00 , G01N35/02 , G01N35/10
CPC分类号： G01N35/109 , B01F2215/0037 , B01L3/0275 , B01L2200/0631 , G01N35/0099 , G01N35/028 , G01N35/1004 , G01N2035/00237 , G01N2035/103 , G01N2035/1058 , Y10T436/255
摘要： PROBLEM TO BE SOLVED: To provide a tool and a method for conveniently collecting nucleic acids from biological samples containing nucleic acids. SOLUTION: Disclosed is a chip for catching nucleic acids having a silica-containing solid phase built in so as to enable to contact with a liquid, wherein the solid phase has a water passage portion and an average gap between the solid phases in the water passage portion is not more than 25 μm. COPYRIGHT: (C)2005,JPO&NCIPI

5. 发明专利

JP2000125847A ORGANISM SPECIMEN-TREATING APPARATUS 失效
公开(公告)号：JP2000125847A
公开(公告)日：2000-05-09
申请号：JP29660598
申请日：1998-10-19
申请人： HITACHI LTD
发明人： FUKUZONO SHINICHI , MAEUCHIHARA NORIE , SAKURAI TOMOYA , YASUDA KENJI
IPC分类号： C12N15/09 , C12M1/00 , C12M1/36 , C12M1/38 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide an automated organism specimen-treating apparatus having a temperature-adjusting device for maintaining a specimen solution containing a treating liquid for isolating an organism molecule from the organism specimen such as an enzyme solution and a surfactant solution at a constant temperature for a set period, and a mechanism for automatically transferring the organism specimen. SOLUTION: This organism specimen-treating apparatus is provided by putting a rectangular holder 1 having specimen liquid in a temperature-adjusting chamber 5 with a sending means A2 and being translated in a vertical direction to a longitudinal direction of the holder 1 by a width of the holder 1 with a sending means B3. The holder 1 sent into the temperature-adjusting chamber is arranged as closely adhering each other in the temperature-adjusting chamber 5. In the temperature-adjusting chamber 5, three holders 1 are placed beforehand, and 4 holders are held in the temperature- adjusting chamber 5. Four rectangular-shaped heaters 7 are arranged in the temperature-adjusting chamber 5 and a temperature adjuster controller 6 controls the temperature. A compensation heater 9 controlled by a temperature compensator controller 8 compensates a corresponding heat value transferred from a holder 1 already in the temperature-adjusting chamber 5 to a new holder sent newly into the temperature-adjusting chamber 5.

6. 发明专利

JP2002191351A APPARATUS FOR PURIFYING NUCLEIC ACID AND CHIP FOR CAPTURING NUCLEIC ACID 审中-公开
公开(公告)号：JP2002191351A
公开(公告)日：2002-07-09
申请号：JP2001321887
申请日：2001-10-19
申请人： HITACHI LTD
发明人： SAKURAI TOMOYA , YASUDA KENJI , MATSUMOTO KOICHI
IPC分类号： C12M1/00
摘要： PROBLEM TO BE SOLVED: To provide an automated apparatus for recovering a nucleic acid with a high degree of purification from a specimen including the nucleic acid. SOLUTION: A dispensing chip 15 connected to a nozzle is used to feed the specimen and a bonding promoter into a treating vessel 24 and a mixed solution in the treating vessel 24 is sucked into a nucleic acid-trapping chip 31 whereby the nucleic acid is trapped to a solid phase embedded in the chip. The eluate is absorbed into the nucleic acid-trapping chip after the solid phase is cleaned and a solution including nucleic acid eluate is collected into a vessel for the purified product 26.

7. 发明专利

JP2001224370A SUPPORT FOR CAPTURING NUCLEIC ACID AND METHOD OF PRODUCING THE SAME 失效
公开(公告)号：JP2001224370A
公开(公告)日：2001-08-21
申请号：JP2000041384
申请日：2000-02-15
申请人： HITACHI LTD
发明人： IKEDA YUKIKO , ENDO KIJU , FUKUZONO SHINICHI , SAKURAI TOMOYA
IPC分类号： C12N15/00 , C12Q1/68 , G01N33/50
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid-capturing support that has high-purity silica efficiently capturing the nucleic acids from sample specimens on the bonding site and a method of inexpensively producing the support. SOLUTION: Silicon fine particles of a prescribed particle size are incorporated into the support skeletons in an arbitrary shape and size, for example, particles, filters, pellets and the like that are adopted to the constitution of individual extractions to form the support to which silica layer having partially uneven thickness are arranged.

8. 发明专利

JP2000336098A APPARATUS FOR PURIFYING NUCLEIC ACID 失效
公开(公告)号：JP2000336098A
公开(公告)日：2000-12-05
申请号：JP14973199
申请日：1999-05-28
申请人： HITACHI LTD
发明人： SUZUKI HIROYUKI , KANEKO YASUO , SAKURAI TOMOYA , MACHIDA KAZUHISA , IZAWA SEIKI
IPC分类号： C12N15/00 , C07H21/00 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide an apparatus for purifying a nucleic acid good in operating efficiency and scarcely inducing maloperation. SOLUTION: This apparatus for purifying a nucleic acid is capable of displaying a nucleic acid extraction screen 108 in an operation panel 2 when completing the start-up operation, transmitting a request for shutdown from the apparatus when a SHUTDOWN button 115 is pressed, displaying the number of specimens set in a specimen rack 10 in a numerical text box 124, changing the number of the specimens with, two triangular buttons 120 and 121 under the text box 124, changing the color of positions where the specimens are present on a specimen rack layout diagram 113 synchronously with the button operation and placing the specimens closely from the upper side to the lower side in the first row from the right on the specimen rack 10 and subsequently from the upper side to the lower side in the second row toward the left side so as not to form an empty site. The specimen positions in the specimen layout diagram 113 are made to correspond to the specimen positions of the specimen rack 10 so as to display the positions of the specimens in the specimen rack 10.

9. 发明专利

JP2000175683A FORMATION METHOD OF CHIP AND STRUCTURED MATERIAL USED FOR ISOLATION OF NUCLEIC ACID 失效
公开(公告)号：JP2000175683A
公开(公告)日：2000-06-27
申请号：JP5974299
申请日：1999-03-08
申请人： HITACHI LTD
发明人： IKEDA YUKIKO , SAKURAI TOMOYA , FUKUZONO SHINICHI , ENDO KIJU , AONO TAKANORI , YOSHIMURA YASUHIRO , TERAYAMA TAKAO , YOKOYAMA TETSUO , YASUDA KENJI
IPC分类号： C12N15/09 , B01D15/00 , C04B38/00 , C04B38/06 , C12M1/00
摘要： PROBLEM TO BE SOLVED: To obtain a chip capable of isolating a nucleic acid from a specimen in a short period of time without a defect such as a cut and in a high recovery rate by including a structured material containing silicon oxides and having holes each with a diameter larger than the silicon oxides, in its flow passage. SOLUTION: This chip used for isolating a nucleic acid, including a structured material containing silicon oxides and having holes each with a diameter larger than the silicon oxides in its flow passage, is obtained by compounding particles 33 of silicon oxides on the surface of a resin particle 32a having a larger diameter than that of the silicon oxides, then putting the compounded particles into a mold 34, heat-treating them at a heat resisting temperature of the resin particles 32a or higher to melt and adhere the resin particles 32a each other for forming a structured material 31, taking out the structured material 31 from the above mold 34, immersing it into a sol.gel solution containing a silicon compound, then taking out the structured material 31 from the sol .gel solution, performing a condensation polymerization of the sol .gel solution thereon by a heat treatment to prepare a structured material 31 capable of capturing the nucleic acid from a liquid specimen and then sealing it in a chip case.

10. 发明专利

JPH11271193A ORGANISM SAMPLE PRETREATING DEVICE 失效
公开(公告)号：JPH11271193A
公开(公告)日：1999-10-05
申请号：JP7697698
申请日：1998-03-25
申请人： HITACHI LTD
发明人： YASUDA KENJI , SAKURAI TOMOYA , MAEUCHIHARA NORIE
IPC分类号： G01N33/48 , C12M1/00 , C12N15/09 , G01N1/10 , G01N1/28 , G01N33/50 , G01N33/53 , G01N35/02
摘要： PROBLEM TO BE SOLVED: To easily and automatically separate genes and nucleic acid from an organism sample containing the genes and the nucleic acid by dissolving gems and virus in the sample, liberating the pieces of the genes and the nucleic acid, and allowing them to adhere to a solid surface. SOLUTION: A sample tube 7 for dissolving germs where a reagent is added is retained for a fixed amount of time, and then a sample is moved to a sample tube 8 for extraction by a dispensing nozzle where a disposable breathing filter built-in tip is fitted. A fixed amount of magnetic fine particles being covered with crystal is added to the sample, and further a chaotropic agent is added. A permanent magnet where the magnetic fine particles are installed at a lower portion is raised, is allowed to adhere to the bottom of a tube, and is collected at the bottom of the tube. Then, a washing liquid is dispensed for washing using a dispensing nozzle where a disposable pipet tip is fitted and finally a small amount of sterilization pure water is added to a sample tube 8, thus dissolving and separating DNA being sucked on the surface of crystal. The dissolved and separated DNA is sucked by a disposable breathing filter built-in tip and is moved to an extraction tube 9 for nucleic acid. The DNA thus obtained is used for each analysis method.

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