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1. 发明专利

JPH046353B2 失效
公开(公告)号：JPH046353B2
公开(公告)日：1992-02-05
申请号：JP12747184
申请日：1984-06-22
申请人： HITACHI LTD
发明人： SHIMIZU NORIO , KURIHARA TOSHIHARU , MASUDA KEIKO , OTAHARA YOJI , BETSUPU TERUHIKO
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/38 , C12N15/71 , C12Q1/68 , C12R1/19

2. 发明专利

JPS619287A Complex plasmid and microorganism having same 失效
标题翻译：复合浆液和具有相同的微生物
公开(公告)号：JPS619287A
公开(公告)日：1986-01-16
申请号：JP12747184
申请日：1984-06-22
申请人： Hitachi Ltd
发明人： SHIMIZU NORIO , KURIHARA TOSHIHARU , MASUDA KEIKO , OTAHARA YOUJI , BETSUPU TERUHIKO
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/38 , C12N15/71 , C12Q1/68 , C12R1/19
CPC分类号： C12Q1/6897 , C07K2319/00 , C07K2319/02 , C07K2319/61 , C12N9/2471 , C12N15/71 , C12Y302/01023
摘要： PURPOSE:A complex plasmid, obtained by linking a tryptophan promoter to beta- galactosidase (beta-gal) gene, useful for producing the beta-gal and further searching for new promoters or assaying the amount expressed of exogenotes by the expression of the beta-gal. CONSTITUTION:A complex plasmid obtained by linking beta-gal gene to a DNA coding 8 amino acids on the N terminal side of trpE peptide in the downstream side of a tryptophan promoter. A microorganism having the above-mentioned complex plasmid is preferably coliform bacillus (Escherichia coil).
摘要翻译：目的：通过将色氨酸启动子与β-半乳糖苷酶（β-gal）基因连接而获得的复合质粒，可用于产生β-gal，并进一步寻找新的启动子，或通过β - gal的表达来测定外源基因的表达量，加仑构成：通过将β-gal基因与在色氨酸启动子的下游侧的trpE肽的N末端侧编码8个氨基酸的DNA连接而获得的复合质粒。具有上述复合质粒的微生物优选为大肠杆菌（Escherichia coil）。

3. 发明专利

JPS619282A Method for cultivating genetic recombinant microorganism 失效
标题翻译：用于培养遗传重组微生物的方法
公开(公告)号：JPS619282A
公开(公告)日：1986-01-16
申请号：JP12747284
申请日：1984-06-22
申请人： Hitachi Ltd
发明人： SHIMIZU NORIO , MASUDA KEIKO , KURIHARA TOSHIHARU , OTAHARA YOUJI
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/38 , C12N15/00 , C12R1/19
摘要： PURPOSE: To obtain efficiently a product of the titled microorganism in a large amount, by cultivating a microorganism having a complex plasmid consisting of the aimed gene, vector and promoter and the expression ability thereof in a culture medium containing an inhibitor substance and an inducer substance to control the promoter activity.
CONSTITUTION: A microorganism having the aimed gene, vector and promoter in the cell and an expression ability of the aimed gene is cultivated in a culture medium containing an inhibitor substance and inducer substance to control the promoter activity and give the product of the aimed gene efficiently in a large amount. For example, a DNA fragment containing a trp promoter cut out of PTREI is linked to an β-gal gene with an enzyme to create a complex plasmid PTREZI, and tryptophan (trp) is added thereto in the initial period of cultivation to suppress the activity of the trp promoter, reduce the production of the β-gal and multiply the microbial cells. An inducer substance for starting the activity of the trp promoter is then added to produce the β-gal.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过培养具有目标基因，载体和启动子组成的复合质粒的微生物及其在含有抑制物质和诱导物质的培养基中的表达能力，有效地获得大量标题微生物的产物以控制启动子活性。构成：在含有抑制剂物质和诱导物质的培养基中培养具有目的基因，细胞中的载体和启动子和靶基因的表达能力的微生物，以控制启动子活性并有效地产生目的基因的产物大量的。例如，含有从PTREI切出的trp启动子的DNA片段与酶的β-gal基因连接以产生复合质粒PTREZI，并且在培养的初始阶段向其中加入色氨酸（trp）以抑制活性的trp启动子，减少β-gal的产生并繁殖微生物细胞。然后加入用于启动trp启动子活性的诱导物质以产生β-gal。

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