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1. 发明专利

JP2006115761A Gene substitution vector targeting birds and its utilization 有权
标题翻译：基因替代矢量定位目标及其应用
公开(公告)号：JP2006115761A
公开(公告)日：2006-05-11
申请号：JP2004306991
申请日：2004-10-21
申请人： Hiroshima Univ , 国立大学法人広島大学
发明人： MATSUDA HARUO , FURUSAWA SHUICHI , HORIUCHI HIROYUKI , KAWASHIMA TAKESHI , SATO MASAHARU
IPC分类号： C12N15/09 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To provide a gene substitution vector for substituting gene of birds in order to realize a system for producing a foreign protein in yolks of birds including domestic fowl and to provide a method for utilizing the gene substitution vector. SOLUTION: The gene substitution vector comprises a gene encoding a part of vitellogenin protein derived from domestic fowl containing a cathepsin D recognition region. The gene encoding an exogenous Enhanced Green Fluorescent Protein (EGFP) is inserted into the cathepsin D recognition region so as to have a base sequence encoding the cathepsin D recognition region at 3' terminal and 5' terminal of the gene. EGFP having the cathepsin D recognition region at N and C terminals is produced as a vitellogenin fused protein by inducing EGFP gene into domestic fowl hepatocyte (LMH) by using the gene substitution vector. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供用于取代鸟类基因的基因取代载体，以实现包括家禽在内的鸟类蛋白中产生外来蛋白质的系统，并提供利用该基因取代载体的方法。解决方案：基因取代载体包含编码源自含有组织蛋白酶D识别区域的家禽的卵黄蛋白原蛋白的一部分的基因。将编码外源增强型绿色荧光蛋白（EGFP）的基因插入组织蛋白酶D识别区域，以具有编码基因3'末端和5'末端的组织蛋白酶D识别区的碱基序列。通过使用基因取代载体，通过将EGFP基因诱导入家禽肝细胞（LMH）而产生具有在N和C末端的组织蛋白酶D识别区的EGFP作为卵黄发生素融合蛋白。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2007167013A Vector for producing objective protein in egg yolk of birds and use thereof 审中-公开
标题翻译：用于生产目标蛋白质的产品用途及其用途
公开(公告)号：JP2007167013A
公开(公告)日：2007-07-05
申请号：JP2005371195
申请日：2005-12-23
申请人： Hiroshima Univ , 国立大学法人広島大学
发明人： MATSUDA HARUO , HORIUCHI HIROYUKI , SATO MASAHARU , FURUSAWA SHUICHI , KAWASHIMA TAKESHI
IPC分类号： C12N15/09 , C12P21/02 , C12P21/06
摘要： PROBLEM TO BE SOLVED: To obtain a vector for producing an objective protein in an egg (especially egg yolk) of a bird including a chicken, to provide a method for producing an objective protein using the vector and a kit containing the vector. SOLUTION: The vector (one example) comprises a promoter and a polynucleotide having a gene encoding a cathepsin D recognition domain, bonded to the 3' terminal side of a gene encoding a domain containing at least a signal sequence and an SE fragment in a chicken-derived vitellogenin protein in which the polynucleotide is controllably bonded to the promoter. The vector has a polynucleotide having a gene encoding a histidine tag, bonded to the 3' terminal side of a gene encoding a cathepsin D recognition domain. The vector contains a poly A addition signal. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：为了获得用于在包括鸡的鸟的卵（特别是蛋黄）中产生目标蛋白质的载体，以提供使用载体生产目标蛋白的方法和含有载体的试剂盒。解决方案：载体（一个实例）包含启动子和具有编码组织蛋白酶D识别结构域的基因的多核苷酸，该多核苷酸与编码至少含有信号序列的结构域和SE片段的基因的3'末端相连接在其中多核苷酸可控地结合到启动子的鸡源性卵黄蛋白原蛋白中。载体具有编码组氨酸标签的基因的多核苷酸，其键合到编码组织蛋白酶D识别结构域的基因的3'末端侧。向量包含poly A加法信号。版权所有（C）2007，JPO＆INPIT

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