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1. 发明专利

JP2014058565A POWDER CONTAINING ANHYDROUS CRYSTAL OF 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID, METHOD FOR PRODUCING THE SAME, AND USES THEREOF 有权
标题翻译：含有2-O-α-D-葡萄糖-L-抗坏血酸的无水晶体的粉末，其制备方法及其用途
公开(公告)号：JP2014058565A
公开(公告)日：2014-04-03
申请号：JP2013262338
申请日：2013-12-19
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C07H17/04 , A23L1/302 , A61K8/00 , A61K8/67 , A61K31/7048 , A61P3/02 , A61Q19/00 , A61Q19/02 , C12P19/00
CPC分类号： C12P19/60 , A23L33/10 , A23L33/15 , A61K8/0241 , A61K8/676 , A61K8/732 , A61K31/7048 , A61K2800/57 , A61K2800/654 , A61Q19/02 , C07H17/04 , Y10T428/2982
摘要： PROBLEM TO BE SOLVED: To provide a powder containing anhydrous crystals of ascorbic acid 2-glucoside, the powder being significantly less prone to caking than existing general-purpose quasi-drug-class powders containing anhydrous crystals of ascorbic acid 2-glucoside, to provide a method for producing the same, and to provide uses thereof.SOLUTION: There are provided a powder containing anhydrous crystals of ascorbic acid 2-glucoside, which contains, in terms of anhydride content, greater than 98.0 mass% and less than 99.9 mass% of ascorbic acid 2-glucoside, and in which the crystallinity of the anhydrous crystals of ascorbic acid 2-glucoside is at least 90%, as calculated on the basis of a powder X-ray diffraction profile, or after free moisture has been removed from the provided powder in a nitrogen stream, the dynamic amount of moisture absorbed by the powder after 12 hours at a temperature of 25°C and a relative humidity of 35 mass% is no more than 0.01 mass%; a method for producing the same; and uses thereof.
摘要翻译：要解决的问题：为了提供含有抗坏血酸2-葡萄糖苷的无水结晶的粉末，与现有的含抗坏血酸2-葡萄糖苷无水晶体的通用准药物级粉末相比，该粉末显着不太容易结块，从而提供其制造方法及其用途。提供了含有抗坏血酸2-葡萄糖苷无水结晶的粉末，其含有以酸酐含量计，大于98.0质量％且小于99.9质量％的抗坏血酸2-葡萄糖苷，其中抗坏血酸2-葡萄糖苷的无水晶体的结晶度为至少90％，基于粉末X射线衍射曲线计算，或者在游离水分从在氮气流中提供的粉末，在25℃的温度和35质量％的相对湿度下12小时之后被粉末吸收的动态水分量不大于0.01质量％; 其制造方法; 及其用途。

2. 发明专利

JP2015052129A プルラン含有粉末とその製造方法並びに用途有权
标题翻译：含PULLULAN的粉末及其制造方法及其应用
公开(公告)号：JP2015052129A
公开(公告)日：2015-03-19
申请号：JP2014253416
申请日：2014-12-15
申请人：株式会社林原 , Hayashibara Co Ltd
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , MIYAKE TOSHIO
IPC分类号： C08B37/00 , A23L29/00
CPC分类号： C12P19/10 , A61K8/022 , A61K8/73 , A61K47/36 , A61K2800/10 , A61Q19/00 , C08B37/0018 , C08L5/00 , C12R1/645
摘要：【課題】溶媒沈殿などによる煩雑な精製工程を経ずに製造され、かつ、フィルムに成形したときに従来品よりも高い破断強度を示すプルラン含有粉末とその製造方法並びに用途を提供することを課題とする。【解決手段】オーレオバシディウム・プルランスに属する微生物の変異株を、炭素源としてグルコース及びマルトースを含む培地で培養して得られる培養物から、夾雑糖質を除去する工程を経ることなく製造されたプルラン含有粉末であって、75容積%水性メタノールに不溶のプルラン画分と可溶の夾雑糖質画分とからなり、アントロン−硫酸法に基づいて求められる、粉末全体に含まれる全糖質含量に対する夾雑糖質画分に含まれる夾雑糖質含量の割合が3質量%以下であるとともに、マンニトールを含有することを特徴とするプルラン含有粉末、及びその製造方法並びに用途を提供することによって上記課題を解決する。【選択図】なし
摘要翻译：要解决的问题：提供含有支链淀粉的粉末，其不经历诸如溶剂沉淀等的麻烦的净化工艺，并且当模制成膜时，其断裂强度高于常规产品的断裂强度，并且制造方法和应用相同。解决方案：通过提供由含有支链淀粉的粉末，由含有支链淀粉的粉末制成的支链淀粉粉末，其由通过在包含葡萄糖和麦芽糖的碳的培养基中培养属于挥发油芽孢杆菌属的微生物的突变体而获得的培养产物作为碳来源是绕过从其中除去杂质糖的方法，其由与75体积％甲醇水溶液不溶的支链淀粉级分和可溶于其的杂质糖类组分组成，相对于包含在整个粉末中的总糖含量的比例，的杂质糖含量中包含的杂质糖含量基于限制在3质量％以下的蒽酮 - 硫酸法计算，包括甘露糖醇及其制造方法和应用。

3. 发明专利

JP2013237698A METHOD FOR PRODUCING POWDERS COMPRISING ANHYDROUS CRYSTALLINE 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID 有权
标题翻译：生产包含无水晶体2-O-α-D-葡萄糖-L-抗坏血酸的粉末的方法
公开(公告)号：JP2013237698A
公开(公告)日：2013-11-28
申请号：JP2013168356
申请日：2013-08-13
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C07H17/04 , A23L33/15 , C12N15/09 , C12P19/44
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing powders containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35 mass%.SOLUTION: A method for producing powders containing anhydrous crystalline ascorbic acid 2-glucoside includes the processes of: allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27 mass%; purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of >86 mass%; precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method; and collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, aging and drying the collected glucoside.
摘要翻译：要解决的问题：提供一种生产含有抗坏血酸2-葡萄糖苷的产率不达到35质量％的无水结晶性抗坏血酸2-葡萄糖苷的粉末的方法，该方法也不显着。解决方案：制备粉末的方法含有无水结晶抗坏血酸2-葡萄糖苷的方法包括：使CGT酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液，然后使葡糖淀粉酶作用于所得溶液以获得具有抗坏血酸的溶液酸2-葡萄糖苷的产率至少为27质量％; 纯化所得溶液以将抗坏血酸2-葡萄糖苷含量提高至> 86质量％的水平; 通过控制冷却法或伪控制冷却法沉淀无水结晶抗坏血酸2-葡萄糖苷; 收集沉淀的无水结晶抗坏血酸2-葡萄糖苷，老化并干燥收集的葡萄糖苷。

4. 发明专利

JP2014139236A METHOD FOR PRODUCING 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID ANHYDROUS CRYSTAL-CONTAINING POWDER 有权
标题翻译：生产2-O-α-D-葡萄糖-L-抗坏血酸含水晶体粉末的方法
公开(公告)号：JP2014139236A
公开(公告)日：2014-07-31
申请号：JP2014082613
申请日：2014-04-14
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C07H17/04 , A23L2/52 , A23L33/15 , A61K8/60 , A61K31/7048 , A61K47/26 , A61P39/06 , A61Q19/00 , C12N15/09 , C12P19/44
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing ascorbic acid 2-glucoside anhydrous crystal-containing powder, which enables production of powder that is hard to solidify significantly, even when a yield of ascorbic acid 2-glucoside produced is less than 35 mass%.SOLUTION: A method for producing ascorbic acid 2-glucoside anhydrous crystal-containing powder comprises the steps of: causing CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid, and causing glucoamylase to act on the solution to obtain a solution in which a percentage of ascorbic acid 2-glucoside produced is 27 mass% or greater; purifying the resulting solution such that the ascorbic acid 2-glucoside content exceeds 86 mass%; precipitating the ascorbic acid 2-glucoside anhydrous crystals by controlled cooling or semi-controlled cooling; and recovering, aging, and drying the precipitated ascorbic acid 2-glucoside anhydrous crystals.
摘要翻译：要解决的问题：为了提供生产抗坏血酸2-葡萄糖苷无水结晶的粉末的方法，即使产生的抗坏血酸2-葡萄糖苷的产率低于35质量％，也能够显着地生产难以固化的粉末％。溶液：制备抗坏血酸2-葡萄糖苷无水结晶粉末的方法包括以下步骤：使CGT酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液，并使葡糖淀粉酶作用于溶液得到生成的抗坏血酸2-葡萄糖苷的百分比为27质量％以上的溶液; 纯化所得溶液使得抗坏血酸2-葡萄糖苷含量超过86质量％; 通过控制冷却或半控冷却沉淀出抗坏血酸2-葡萄糖苷无水晶体; 并回收，老化和干燥沉淀的抗坏血酸2-葡萄糖苷无水晶体。

5. 发明专利

JP2014015469A POWDER CONTAINING ANHYDROUS CRYSTAL OF 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID, METHOD FOR PRODUCING THE SAME, AND USE THEREOF 有权
标题翻译：含有2-O-α-D-葡萄糖-L-抗坏血酸的无水晶体的粉末，其生产方法及其用途
公开(公告)号：JP2014015469A
公开(公告)日：2014-01-30
申请号：JP2013178683
申请日：2013-08-29
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： A61K8/67 , A23L1/30 , A61Q1/12 , A61Q19/02
CPC分类号： C12P19/60 , A23L33/10 , A23L33/15 , A61K8/0241 , A61K8/676 , A61K8/732 , A61K31/7048 , A61K2800/57 , A61K2800/654 , A61Q19/02 , C07H17/04 , Y10T428/2982
摘要： PROBLEM TO BE SOLVED: To provide powder containing anhydrous crystals of ascorbic acid 2-glucoside, the powder being significantly less prone to caking than existing general-purpose quasi-drug-class powder containing anhydrous crystals of ascorbic acid 2-glucoside, to provide a method for producing the same, and to provide use thereof.SOLUTION: Powder containing anhydrous crystals of ascorbic acid 2-glucoside contains, in terms of anhydride content, greater than 98.0 mass% and less than 99.9 mass% of ascorbic acid 2-glucoside, and the crystallinity of the anhydrous crystals of ascorbic acid 2-glucoside is 90% or more, as calculated on the basis of a powder X-ray diffraction profile, or after free moisture has been removed from the powder in a nitrogen stream, the dynamic amount of moisture absorbed by the powder after 12 hours at a temperature of 25°C and a relative humidity of 35 mass% is 0.01 mass% or less. A method for producing the same and use thereof are also provided.
摘要翻译：要解决的问题：为了提供含有抗坏血酸2-葡萄糖苷无水结晶的粉末，与现有的含抗坏血酸2-葡萄糖苷无水晶体的通用准药剂级粉末相比，该粉末显着不太容易结块，从而提供其制造方法及其用途。溶液：含有抗坏血酸2-葡萄糖苷的无水结晶的粉末含有以酸酐含量计，抗坏血酸2-葡萄糖苷含量大于98.0质量％且小于99.9质量％抗坏血酸2-葡萄糖苷的无水结晶的结晶度为90％以上，基于粉末X射线衍射曲线计算，或者在氮气流中从粉末中除去游离水分后，动态在25℃，相对湿度35质量％下12小时后粉末吸收的水分量为0.01质量％以下。还提供了其制备方法及其用途。

6. 发明专利

JP2013059316A METHOD FOR PRODUCING 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID ANHYDROUS CRYSTAL-CONTAINING POWDER 有权
标题翻译：生产2-O-α-D-葡萄糖-L-抗坏血酸含水晶体粉末的方法
公开(公告)号：JP2013059316A
公开(公告)日：2013-04-04
申请号：JP2012153260
申请日：2012-07-09
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C12P19/18 , A23L33/15 , C07H1/06 , C07H15/26 , C12N9/10 , C12N15/09
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder which can significantly produce hardly solidifying powder even when the formation rate of ascorbic acid 2-glucoside is less than 35 mass%.SOLUTION: The method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder includes the steps of: making CGTase and starch debranching enzyme act to a solution containing either liquefied starch or dextrin and L-ascorbic acid and subsequently making glycoamylase on the same in order to obtain a solution in which the formation rate of ascorbic acid 2-glucoside is 33 mass% or greater; purifying the obtained solution such that the ascorbic acid 2-glucoside content exceeds 86 mass%; precipitating the ascorbic acid 2-glucoside anhydrous crystals by a controlled cooling method or a semi-controlled cooling method; and recovering, aging, and drying the precipitated ascorbic acid 2-glucoside anhydrous crystals.
摘要翻译：待解决的问题：提供即使当抗坏血酸2-葡萄糖苷的形成速率小于35质量％时，也可以生产几乎不凝固的粉末的抗坏血酸2-葡萄糖苷无水结晶粉末的制造方法。解决方案：制备抗坏血酸2-葡萄糖苷无水结晶粉末的方法包括以下步骤：使CGTase和淀粉脱支酶作用于含有液化淀粉或糊精和L-抗坏血酸的溶液，随后制备糖酵解酶相同，以获得其中抗坏血酸2-葡萄糖苷的形成速率为33质量％以上的溶液; 纯化所得溶液使得抗坏血酸2-葡萄糖苷含量超过86质量％; 通过控制冷却方法或半控冷却方法沉淀抗坏血酸2-葡萄糖苷无水晶体; 并回收，老化和干燥沉淀的抗坏血酸2-葡萄糖苷无水晶体。版权所有（C）2013，JPO＆INPIT

7. 发明专利

JP2013055932A METHOD FOR PRODUCING POWDER CONTAINING 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID ANHYDROUS CRYSTAL 有权
标题翻译：含有2-O-α-D-葡萄糖酸-L-抗坏血酸无水晶体的粉末的方法
公开(公告)号：JP2013055932A
公开(公告)日：2013-03-28
申请号：JP2012153259
申请日：2012-07-09
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C12P19/18 , A23L33/15 , C07H17/04 , C12N15/09
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing the powder containing an ascorbic acid 2-glucoside anhydrous crystal with which it is possible to produce a powder that will not show significant solidification, even when the production percentage of ascorbic acid 2-glucoside is less than 35 mass%.SOLUTION: A method for producing the powder containing an ascorbic acid 2-glucoside anhydrous crystal comprises: a step in which a solution containing either liquefied starch or dextrin and L-ascorbic acid is exposed to CGTase and then to glucoamylase in order to obtain a solution wherein the production percentage of ascorbic acid 2-glucoside is 27 mass% or greater; a step of purifying the resulting solution such that the ascorbic acid 2-glucoside content exceeds 86 mass%; a step of precipitating the ascorbic acid 2-glucoside anhydrous crystals by controlled cooling or semi-controlled cooling; and a step of recovering, aging, and drying the precipitated ascorbic acid 2-glucoside anhydrous crystals.
摘要翻译：待解决的问题：为了提供含有抗坏血酸2-葡萄糖苷无水结晶的粉末的制造方法，即使生产不会显着凝固的粉末，即使生产抗坏血酸2 葡糖苷的含量小于35质量％。解决方案：含有抗坏血酸2-葡萄糖苷无水晶体的粉末的制造方法包括：将含有液化淀粉或糊精和L-抗坏血酸的溶液暴露于CGT酶，然后暴露于葡糖淀粉酶的步骤，以便得到抗坏血酸2-葡萄糖苷的生成百分比为27质量％以上的溶液，纯化所得溶液使得抗坏血酸2-葡萄糖苷含量超过86质量％的步骤; 通过控制冷却或半控冷却沉淀出抗坏血酸2-葡萄糖苷无水晶体的步骤; 以及回收，老化和干燥沉淀的抗坏血酸2-葡萄糖苷无水晶体的步骤。版权所有（C）2013，JPO＆INPIT

8. 发明专利

JP2015025012A α，α−トレハロース二含水結晶含有粉末の製造方法有权
公开(公告)号：JP2015025012A
公开(公告)日：2015-02-05
申请号：JP2014217192
申请日：2014-10-24
申请人：株式会社林原 , Hayashibara Co Ltd
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE
IPC分类号： C07H3/04 , C13B50/00
CPC分类号： C07H3/04 , C07H1/00 , C12N9/1074 , C12P19/12 , C12P19/14 , C12P19/18 , C12P19/20 , C12Y204/01019 , C13K13/007
摘要：【課題】トレハロース二含水結晶含有粉末を、澱粉を原料に、一貫した工程で、対澱粉収率良く製造することを可能にする製造方法を提供する。【解決手段】液化澱粉に、澱粉枝切酵素及びシクロマルトデキストリン・グルカノトランスフェラーゼとともに、α−グリコシルトレハロース生成酵素とトレハロース遊離酵素を作用させ、次いで、グルコアミラーゼを作用させてα,α−トレハロース含有糖液を得、前記糖液からα,α−トレハロース二含水結晶を晶析させ、これを採取、熟成、乾燥する工程を含み、前記シクロマルトデキストリン・グルカノトランスフェラーゼとしてパエニバチルス属微生物由来の天然型若しくは組換え型酵素又はそれらの変異体酵素を用いることにより、カラムクロマトグラフィーによる分画工程を経ることなく、前記糖液中のα,α−トレハロース含量を無水物換算で86.0質量%超とすることを特徴とするレハロース二含水結晶含有粉末の製造方法を提供することにより解決する。【選択図】なし

9. 发明专利

JP2013151556A METHOD FOR PRODUCING 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID ANHYDROUS CRYSTAL-CONTAINING POWDER 有权
公开(公告)号：JP2013151556A
公开(公告)日：2013-08-08
申请号：JP2013094927
申请日：2013-04-30
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C07H17/04 , A23L33/15 , C12P19/44
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder, capable of producing a powder that will not show significant solidification, even when the percentage of ascorbic acid 2-glucoside produced is less than 35 mass%.SOLUTION: A method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder comprises: a step in which a solution containing either liquefied starch or dextrin and L-ascorbic acid is exposed to CGTase and then to glucoamylase in order to obtain a solution where the percentage of ascorbic acid 2-glucoside produced is 27 mass% or greater; a step of purifying the resulting solution such that the ascorbic acid 2-glucoside content exceeds 86 mass%; a step of precipitating the ascorbic acid 2-glucoside anhydrous crystals by controlled cooling or semi-controlled cooling; and a step of recovering, aging, and drying the precipitated ascorbic acid 2-glucoside anhydrous crystals.

10. 发明专利

JP2013151555A METHOD FOR PRODUCING 2-O-α-D-GLUCOSYL-L-ASCORBIC ACID ANHYDROUS CRYSTAL-CONTAINING POWDER 有权
标题翻译：生产2-O-α-D-葡萄糖-L-抗坏血酸含水晶体粉末的方法
公开(公告)号：JP2013151555A
公开(公告)日：2013-08-08
申请号：JP2013094917
申请日：2013-04-30
申请人： Hayashibara Co Ltd , 株式会社林原
发明人： SHIBUYA TAKASHI , IZAWA SEISUKE , NISHIMOTO TOMOYUKI , FUKUDA SHIGEHARU , MIYAKE TOSHIO
IPC分类号： C07H17/04 , A23L33/15 , C12N15/09
CPC分类号： A61K31/7048 , A61K9/16 , C07H17/04 , C12P19/18 , C12P19/60 , C12Y204/01019
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder, capable of producing a powder that will not show significant solidification, even when the percentage of ascorbic acid 2-glucoside produced is less than 35 mass%.SOLUTION: A method for producing an ascorbic acid 2-glucoside anhydrous crystal-containing powder comprises: a step in which a solution containing either liquefied starch or dextrin and L-ascorbic acid is exposed to CGTase and then to glucoamylase in order to obtain a solution where the percentage of ascorbic acid 2-glucoside produced is 27 mass% or greater; a step of purifying the resulting solution such that the ascorbic acid 2-glucoside content exceeds 86 mass%; a step of precipitating the ascorbic acid 2-glucoside anhydrous crystals by controlled cooling or semi-controlled cooling; and a step of recovering, aging, and drying the precipitated ascorbic acid 2-glucoside anhydrous crystals.
摘要翻译：要解决的问题：即使产生的抗坏血酸2-葡萄糖苷的百分比小于35，也可以提供能够生产不显示固化的粉末的抗坏血酸2-葡萄糖苷无水结晶粉末的制造方法质量％溶液：制备抗坏血酸2-葡萄糖苷无水结晶粉末的方法包括：将含有液化淀粉或糊精和L-抗坏血酸的溶液暴露于CGT酶然后暴露于葡糖淀粉酶的步骤，以便得到生成的抗坏血酸2-葡萄糖苷的百分比为27质量％以上的溶液; 纯化所得溶液使得抗坏血酸2-葡萄糖苷含量超过86质量％的步骤; 通过控制冷却或半控冷却沉淀出抗坏血酸2-葡萄糖苷无水晶体的步骤; 以及回收，老化和干燥沉淀的抗坏血酸2-葡萄糖苷无水晶体的步骤。

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