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    • 3. 发明专利
    • Cell culture apparatus and cell culture method
    • 细胞培养装置和细胞培养方法
    • JP2003000225A
    • 2003-01-07
    • JP2001191899
    • 2001-06-25
    • Hakuju Inst For Health Science Co Ltd株式会社白寿生科学研究所
    • DOKAI FUYUKIISAKA KATSUOHARA AKIKUNI
    • C12M3/00C12M1/00C12M1/42C12M3/06C12N5/06C12N13/00
    • C12M35/02
    • PROBLEM TO BE SOLVED: To provide a cell culture apparatus and a cell culture method to enable uniform culture of cells by uniformizing electric current density.
      SOLUTION: The culture apparatus is provided with a vessel 14 having a prescribed capacity and holding a lower electrode 16 at the bottom, a lid 17 put on the vessel 14 to seal the vessel, having an upper electrode 18 placed opposite to the lower electrode 16 and positioned opposite to the vessel 14 in vertical direction, a culture member 20 inserted into the vessel 14 and containing a hollow part 21 having a volume with a prescribed cross-sectional area and positioned nearly at the center, a filter 25 placed between the vessel 14 and the culture member 20, and a power source to pass electric current by applying a prescribed voltage between the lower electrode 16 and the upper electrode 18.
      COPYRIGHT: (C)2003,JPO
    • 要解决的问题:提供细胞培养装置和细胞培养方法,以通过使电流密度均匀化来均匀培养细胞。 解决方案:培养装置具有规定容量的容器14,并且在底部保持下部电极16,将盖17放置在容器14上以密封容器,具有与下部电极16相对设置的上部电极18 并且在垂直方向上与容器14相对定位;培养构件20,其插入容器14中,并容纳具有规定横截面面积并且几乎位于中心的体积的中空部分21;过滤器25,设置在容器 14和培养构件20,以及通过在下部电极16和上部电极18之间施加规定的电压来使电流通过的电源。