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1. 发明专利

JPS63100377A METHOD FOR REFINING REAGENT FOR IMMUNE MEASUREMENT 失效
公开(公告)号：JPS63100377A
公开(公告)日：1988-05-02
申请号：JP24526486
申请日：1986-10-17
申请人： HITACHI LTD
发明人： TSUBAKIMOTO HIROKO , SAGUSA TOSHIYUKI
IPC分类号： G01N30/88 , B01D15/08 , G01N33/531 , G01N33/543
摘要： PURPOSE:To permit inexpensive refining of a reagent for immune measurement and exact measurement by refining the reagent by column chromatography packed with the solid phase of the same material as the material of a flow passage valve of a reagent dispensing mechanism. CONSTITUTION:A pump 1 is used to pass antiserum which is the reagent for measurement or buffer soln. Alumina powder which constitutes the flow rate selector valve of an automatic analyzer is packed into the column 2. The reagent for IgG measurement is first passed in order to equilibrate ceramic particles in the column chromatography constituted in the above-mentioned manner. Then antiserum which is the reagent for the IgG measurement is then injected into the column and is adsorbed to the ceramic packing material in the upper part of the column 2. The buffer soln. is thereafter passed to separate antibody and antigen-like impurities. The column 2 is regenerated while the column is kept monitored by a detecting part 3. The eluate thereof is used as the reagent for the IgG measurement. The inexpensive refining of the reagent is thereby permitted; in addition, nonspecific agglutination is consequently prevented and the exact measurement based on the antigen-antibody reaction is permitted.

2. 发明专利

JPS6312964A METHOD AND INSTRUMENT FOR AUTOMATIC DISCRETE ANALYSIS 失效
公开(公告)号：JPS6312964A
公开(公告)日：1988-01-20
申请号：JP15605386
申请日：1986-07-04
申请人： HITACHI LTD
发明人： SAGUSA TOSHIYUKI
IPC分类号： G01N35/02 , G01N21/75 , G01N35/00
摘要： PURPOSE:To save manpower for remeasurement by bringing a sample and reagents into reaction to obtain a measured value and retreat the sample automatically according to the defectiveness of the measurement results by a control device. CONSTITUTION:The sample of a specimen vessel 1 of a sample table 2 is put into a reaction vessel 18 of a reaction table 8 by a pipetting mechanism 6. The reagents 22, 23 are put into the reaction vessel 18 and are brought into reaction with the sample. The absorbancy of the sample is then measured by a spectroscope 12. The measurement operation is controlled by a controller 28. A systematic trouble (S) with which the measured values are continuously defective and a random trouble (R) with which the measured values are contingently defective are judged by the controller 28 at this time by the controller 28 and the cause thereof is indicated. The measurement conditions, etc., are corrected to the normal conditions, etc., and the measurement is made again in the case of the trouble (S). The sample is diluted with a diluent and the measurement is made again after the sample is diluted with a liquid diluent if, in the case of the trouble (R), the cause is, for example, the abnormally high activity of the sample. The sample is, therefore, automatically treated and the measurement is made again according to the cause of the defect of the measurement results. The time and manpower for the remeasurement treatment are thus saved.

3. 发明专利

JPS6244169A DEVICE FOR MEASURING ENZYME 失效
公开(公告)号：JPS6244169A
公开(公告)日：1987-02-26
申请号：JP18428285
申请日：1985-08-23
申请人： HITACHI LTD
发明人： YOKOSE TAIZO , SAGUSA TOSHIYUKI
IPC分类号： G01N21/75 , C12M1/34
摘要： PURPOSE:An enzyme measuring device having the same performance of a photometric system even if a product is changed, by using a cell which has a reaction solution and is made of hard glass having improved permeability to ultraviolet light and by attaching a filter to a light detector which makes light rays emitted from a light source and transmitted through the cell into a monochromatic light and detecting same. CONSTITUTION:Light rays which emitted from the light source 13 and transmitted through the cell (square rector) 18 having a reaction solution is introduced to the spectrograph 12, make into monochromatic light and transmitted light intensity is detected by the detector 15. An enzyme in the reaction solution is measured by using absorption of a coenzyme approximately at 340nm wavelength. In this case, a tungesten iodide lamp is used s the light source 13 and hard glass having improved permeability to ultraviolet light is used as a material for the cell 18. The filter 16 which reduces light energy of light rays with >=400nm wavelength and makes the energy equal to light energy approximately at light rays approximately at 340nm is set in front of the detector 15. My the above-mentioned constitution, stable measured values are obtained even by setting a subsidiary wavelength at the outside of the absorption beam of the coenzyme and measured data having the same level are obtained even if a device is different.

4. 发明专利

JPS61247377A CULTURE VESSEL FOR MEASURING MICROORGANISMS AND DETECTION OF MICROORGANISM THEREWITH 失效
公开(公告)号：JPS61247377A
公开(公告)日：1986-11-04
申请号：JP8868085
申请日：1985-04-26
申请人： HITACHI LTD , HITACHI INSTRUMENTS ENG
发明人： SAGUSA TOSHIYUKI , MAKIGUCHI HIROKO
IPC分类号： G01N33/48 , C12M1/34 , C12Q1/04 , G01N33/15
摘要： PURPOSE:The title vessel for measuring microorganisms is formed with blocks having cultivation wells which have a downward convex surface at the bottom so that it has a horizontal surface of a specified magnitude of the area of the parallel beam used at the bottom center whereby accurate and rapid inspection of bacterial becomes possible. CONSTITUTION:The cultivation vessel for measuring microorganisms 2 is com posed of cultivation wells which are made of light-transmitting material and have downward convex bottom surface so that the area of the horizontal surface is 3-1,000 times that of the parallel beam which is used at the bottom center. The parallel beam from the laser source 1 is irradiated to nearly the center of the bottom surface of the vessel 2 and the forward scattering light is mea sured through the blocker 4. According to the present invention, microorganisms are measured rapidly with high sensitivity.

5. 发明专利

JPS60196669A Analytical method and apparatus for antigen-antibody reaction 失效
标题翻译：抗原反应的分析方法和装置
公开(公告)号：JPS60196669A
公开(公告)日：1985-10-05
申请号：JP5214284
申请日：1984-03-21
申请人： Hitachi Ltd , Nitsusui Seiyaku Kk
发明人： MAKIGUCHI KIYOUKO , SAGUSA TOSHIYUKI , NOMURA YASUSHI , NAKA YUTAKA , SAWAI MASATOSHI
IPC分类号： G01N33/536 , A61K39/00 , G01N21/75 , G01N33/543 , G01N35/02
CPC分类号： G01N21/75
摘要： PURPOSE:To confirm whether a first measured value is one in a reaction substance excessive region, by adding the same reagent to a first reaction solution, after the first measured value was obtained, to obtain a second reaction solution and obtaining a second measured value relating to said second reaction solution. CONSTITUTION:After first measurement is finished and a reaction container 6 is transferred to a second reagent adding position, a predetermined amount of a second reagent solution containing the same antibody as a first reagent solution is added through the pipe 10 of a supply mechanism 9. By adding the additional solution as mentioned above, the production amount of an immune composite is changed. The second reaction solution receives the measurement of absorbancy at a second photometric position by second photometers 12b, 11b are necessary wavelength light receives photoelectric conversion. The converted signal is guided to a microcomputer 20 through an A/D converter 15b and the change in absorbancy based on the change in a liquid amount by the addition of the additional solution is corrected and the second measured value relating to the same specimen is compared with the first measured value to judge the presence or absence of influence of a prozone phenomenon on the basis of a predetermined reference.
摘要翻译：目的：为了确认反应物质过量区域中的第一测定值是否为1，通过向第一反应溶液添加相同的试剂，得到第一反应溶液，得到第二反应溶液，得到第二测定值至所述第二反应溶液。构成：第一次测定结束后，将反应容器6转移到第二试剂添加位置，通过供给机构9的配管10添加规定量的含有与第一试剂溶液相同的抗体的第二试剂溶液。通过加入如上所述的附加溶液，改变免疫复合物的产生量。第二反应溶液通过第二光度计12b，11b接收在第二测光位置处的吸光度的测量，波长光需要受光电转换。转换后的信号通过A / D转换器15b被引导到微型计算机20，并且通过添加附加溶液基于液体量的变化而改变吸收度，并将与相同样品相关的第二测量值进行比较以第一测量值为基础，判断是否存在前区现象的影响。

6. 发明专利

JPS60152954A MEASUREMENT OF BIOLOGICAL SAMPLE 失效
公开(公告)号：JPS60152954A
公开(公告)日：1985-08-12
申请号：JP899784
申请日：1984-01-20
申请人： HITACHI LTD
发明人： MAKIGUCHI KIYOUKO , SAGUSA TOSHIYUKI
IPC分类号： G01N33/543 , G01N21/25
摘要： PURPOSE:To enable a highly accurate measurement of a biological sample by separating a carrier from a vapor phase through a centrifugal force to measure optical characteristic based on material in the vapor phase. CONSTITUTION:A plurality of reaction vessels 2 having a trapping section each are set on a reaction table 1 and a plurality of sample cups 4 containing a standard substance and a sample to be inspected are set on a sample table 3 separately. A sample distribution arm 5 discharges a sample liquid at the fixed position 6 on the table 3 into a vessel 2 at the fixed position 7 on the table 1. The table 1 is fed rotatively by steps according to intervals between the vessels 2 and the table 3 is turned based on input information on analysis items. As each vessel 2 reaches the specified position 7, arms 8 and 9 move to discharge a reagent of a cold insulation storage 10 into the vessel 2 to mix the sample with the reagent to react. Then, the table 1 is turned at a high speed to separate a fluorescent labeled substance bonded to a solid phase from an isolated fluorescent labeled substance and then, the vessel 2 is irradiated with a laser light to indicate the results of analysis on a printer 14 and a CRT15 through an A/D converter 40. These operations are controlled with a microcomputer 11 based on analysis conditions previously inputted.

7. 发明专利

JPS6035241A CHROMOGEN MEASURING METHOD 失效
公开(公告)号：JPS6035241A
公开(公告)日：1985-02-23
申请号：JP12371684
申请日：1984-06-18
申请人： HITACHI LTD
发明人： SAGUSA TOSHIYUKI , NOMURA YASUSHI , YABE RIYOUHEI
IPC分类号： G01N21/27 , G01N21/31 , G01N21/75 , G01N33/49 , G01N35/02
摘要： PURPOSE:To determine three kinds of Chromogens of chyle, haemolysis and yellow from the same sample simultaneously, by measuring absorbances in a long wavelength region, in an intermediate wavelength region, and in a short wavelength region of a visual wavelength region. CONSTITUTION:Curves 20, 22 and 24 are absorption spectrums of a chyle reference liquid, a haemolysis reference liquid and a yellow reference liquid, which are diluted by a GOT measuring liquid, respectively. The degree of the chyle is obtained from the absorbance in a long wavelength region (e.g., lambda20 and lambda21) in a visual wavelength region, where the effect of the chyle exists but the effects of the haemolysis and the yellow does not actually exist. The degree of the haemolysis is obtained based on the absorbance in the long wavelength region and the absorbance in an intermediate wavelength region (e.g., lambda18 and lambda19), where the effect of the yellow does not actually exist. The degree of the yellow is obtained from the absorbance in the intermediate wavelength region and the absorbance in a short wavelength region (e.g., lambda15 and lambda16).

8. 发明专利

JPS6027398A EXAMINATION OF MICROORGANISM 失效
公开(公告)号：JPS6027398A
公开(公告)日：1985-02-12
申请号：JP13656583
申请日：1983-07-26
申请人： HITACHI LTD
发明人： MAKIGUCHI KIYOUKO , SAGUSA TOSHIYUKI , NOMURA YASUSHI
IPC分类号： G01N33/52 , C12Q1/02 , C12Q1/04 , G01N21/76 , G01N21/77
摘要： PURPOSE:To carry out examination in high accuracy rapidly, by adding a reagent to be treated with an esterase and to be converted into a fluorescent substance to a culture solution obtained by cultivating a specimen, keeping it warm, measuring the number of microorganisms depending upon an amount of liberated fluorescent substance. CONSTITUTION:A common mold such as bacterium, yeast, filamentous fungi, etc. absorbs a 2,3-dicyanobenzene derivative such as non-fluorescent 1,4-diacetoxy-2, 3-dicyanobenzene shown by the formula, etc. in the cell, it is decomposed with an esterase in the cell, and a fluorescent substance (2,3-dicyano 1,4-hydroxy) not to permeate through a cell wall is liberated. In the process, the intensity of fluorescence of the reaction solution is proportional to the number of commono live molds. A test solution containing a fixed amount of a specimen is reacted with the nonfluorescent 2,3-dicyano-benzene derivative, the number of microorganisms is measured depending upon the intensity of fluorescence of the reaction solution, so that the change in number of live molds of microorganism in the specimen is detected in high accuracy in a short time.

9. 发明专利

JPS5587030A REACTION RATE MEASURING INSTRUMENT 失效
公开(公告)号：JPS5587030A
公开(公告)日：1980-07-01
申请号：JP15858078
申请日：1978-12-25
申请人： HITACHI LTD
发明人： MATSUOKA YOSHIO , SAGUSA TOSHIYUKI , KIDA TAKASHI
IPC分类号： G01N21/75 , G01N21/27 , G01N21/77
摘要： PURPOSE:To make high-precision analyses even if the density of an item to be inspected would be low, by optically measuring time variation of chemical reaction by a mechanism which leads a reagent bland solution to a flow cell and then leads a sample solution to be inspected to the same flow cell. CONSTITUTION:As for a reaction speed measuring instrument which makes quantitative analyses of an item to be analyzed in an inspected sample by making an additive reagent react upon the inspected sample and by measuring optically the time variation of the chemical reaction, a reagent blank solution and sample solution are alternated on reaction line 18 and at the start of measurement, the reagent blank solution is led to cell 9 to measure its transmitting quantity by detector 14. Next, the inspected sample is led to cell 9 to measure its transmitting light quantity similarly. From a difference between a transmitting light quantity based upon the inspected sample solution and that based upon the reagent blank solution, absorbance is found by arithmetic part 16.

10. 发明专利

JPS5523445A MEASURING METHOD FOR REACTION RATE OF CREATININE 失效
公开(公告)号：JPS5523445A
公开(公告)日：1980-02-19
申请号：JP9616978
申请日：1978-08-09
申请人： HITACHI LTD
发明人： SAGUSA TOSHIYUKI , NOMURA YASUSHI
IPC分类号： G01N21/77 , G01N21/75 , G01N33/70
摘要： PURPOSE:To evade a negative error from coexisting bilirubin by measuring a sample after incuvation with a specific oxidizer to the sample, in a method of calculating the density of creatinine from the formation rate of creatinine picrate. CONSTITUTION:In an alkaline atmosphere, an organic-chlorine-based oxidizer such as dichloroisocyanuric acid, chloramine-B, chloramine-T, and sodium hyprochlorite is added to a formed sample and after incuvation, picric acid is added, thereby ending the formation of picramic acid. At the point in time when reaction of picric acid upon protein and acetone bodies stops, the formation rate of creatinine picrate is found by making use of variation in photo absorptivity of about 500nm, thereby measuring the density of creatinine.

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