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    • 1. 发明专利
    • Method for producing protein
    • 生产蛋白质的方法
    • JP2011000127A
    • 2011-01-06
    • JP2010198550
    • 2010-09-06
    • Fujirebio Inc富士レビオ株式会社
    • INABA JIROHORI TAKEYAITO SATORU
    • C12P21/02C12N9/10C12N15/09
    • Y02P20/52
    • PROBLEM TO BE SOLVED: To provide a method for producing a desired protein by gene recombination technology which is the method for producing the desired protein by the gene recombination technology and by which the desired produced protein can be easily recovered without denaturation.SOLUTION: The method includes: introducing a vector, in which a fused gene containing a gene encoding a multiple pass transmembrane-type protein and a desired gene is combined, into virus particle-producing host cells to express the fused gene in the host cells; producing the desired protein in a form fused to the virus particles and the multiple pass transmembrane-type protein; and recovering the virus particles fused to the desired protein.
    • 要解决的问题:提供一种通过基因重组技术生产所需蛋白质的方法,该技术是通过基因重组技术生产所需蛋白质的方法,并且可以容易地在不变性的情况下回收所需生产的蛋白质。方法:该方法 包括:将含有编码多次跨膜型蛋白质的基因的融合基因和所需基因组合的载体导入到产生宿主细胞的病毒颗粒中,以在宿主细胞中表达融合基因; 以与病毒颗粒和多次跨膜型蛋白质融合的形式产生所需蛋白质; 并回收与所需蛋白质融合的病毒颗粒。