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1. 发明专利

JPH01161000A NEUTROPHIL-ACTIVATING FACTOR, ITS PRODUCTION AND ANTITUMOR AGENT AND ANTIBACTERIAL AGENT CONTAINING SAID FACTOR 失效
公开(公告)号：JPH01161000A
公开(公告)日：1989-06-23
申请号：JP31832887
申请日：1987-12-16
申请人： EISAI CO LTD
发明人： SHIONOYA HIROSHI , KOYANAGI NOZOMI , SATO TOSHITAKA , KUWATA MANABU , KOIDE JUN , MIYOSHI ISAO
IPC分类号： A61K38/00 , A61P1/04 , A61P31/04 , C07K1/20 , C07K14/00 , C07K14/52 , C12P21/00 , C12R1/91
摘要： NEW MATERIAL:A neutrophil-activating factor having a molecular weight of about 25,000 and containing a fragment having amino acid sequences of formula I-IV. USE:An antitumor agent and antibacterial agent. PREPARATION:An MT-2 cell which is an antigen-producing cell strain originated from human umbilical leukocyte is established by the mixed culture with leukemia cell collected from the peripheral blood of an adult T-cell leukemia patient. The MT-2 cell is cultured in a medium containing bovine fetus serum, etc., and the cultured liquid is centrifuged to precipitate and separate the cells and obtain the supernatant liquid. The supernatant liquid of the cultured product is passed through a molecular sieve membrane, concentrated, dialyzed, subjected to ion exchange column chromatography and eluted by the concentration gradient of sodium chloride. The eluted active fraction is purified by gel filtration and reversed phase high-performance liquid chromatography, etc., to obtain the objective neutrophil activating factor.

2. 发明专利

JPH01113663A MEASURING REAGENT OF ATLV ANTIBODY AND MEASURING METHOD THEREOF 失效
公开(公告)号：JPH01113663A
公开(公告)日：1989-05-02
申请号：JP27203587
申请日：1987-10-28
申请人： EISAI CO LTD
发明人： SAWADA TAKASHI , FUJIMATSU JUNICHI , MIYOSHI ISAO , TAGUCHI HIROKUNI
IPC分类号： C12Q1/00 , G01N33/548 , G01N33/569 , G01N33/574
摘要： PURPOSE:To enable the accurate measurement of an ATLV antibody, by using a transferred nitrocellulose membrane of an ATL-related antigen and a measuring reagent containing an ATLV antigen as a requisite component, and by adding this reagent to a sample to be inspected, so as to be subjected to band comparison. CONSTITUTION:A measuring reagent and a measuring method for determining an ATLV antibody in a sample to be inspected, a serum or the like, by using an ATL- related antigen are proposed. In more detail, the ATL-related antigen is differentiated according to a molecular weight beforehand by an electrophoretic method and transferred onto a nitrocellulose membrane. The sample to be inspected is added to the transferred nitrocellulose membrane, while an absorbing sample prepared by adding an ATLV antigen to the sample and making adjustment is added thereto, and a band appearing in each of them is compared with another. Only by this comparison, the existence of the ATLV antibody in the sample to be inspected can be determined in a convenient and simple manner. The ATLV-related antigen can be obtained by a method wherein an antigen-produced live cell strain called MT-2, which is derived from a human umbilical leukocyte obtained from the mixing culture of leukemia cells, is treated by using a surfactant.

3. 发明专利

JPS5962527A Preparation of antigen relating to adult t-cell leukemia 失效
标题翻译：与成人T细胞淋巴细胞相关的抗原的制备
公开(公告)号：JPS5962527A
公开(公告)日：1984-04-10
申请号：JP16967082
申请日：1982-09-30
申请人： Eisai Co Ltd
发明人： SAWADA TAKASHI , MORIMOTO TOMIAKI , MIYOSHI ISAO , TAGUCHI HIROKUNI , FUJIMATSU JIYUNICHI , KITAMURA TOYOHIRO
IPC分类号： G01N33/574 , A61K35/12 , A61K35/14 , A61K39/00 , A61K39/21 , G01N33/531 , G01N33/569
CPC分类号： G01N33/531 , A61K39/00 , G01N33/56972 , G01N33/56988 , Y10S435/81 , Y10S435/948 , Y10S435/961 , Y10S435/975 , Y10S436/804 , Y10S436/808 , Y10S436/811 , Y10S436/813
摘要： PURPOSE: To prepare the titled antigen by stirring the cells capable of producing the antigen relating to adult T-cell leukemia in the presence of a surface active agent.
CONSTITUTION: Cells capable of producing the antigen relating to adult T-cell leukemia (e.g. MT-2 cell) are stirred in the presence of a surface active agent (e.g. sodium deoxycholate, octylphenoxy polyethoxy-ethanol, etc.), the mixture is centrifuged, and the supernatant liquid is dialyzed using physiological saline water buffered with phosphoric acid. The surface active agent contained in the suprenatant liquid can be removed by the dialysis. The dialyzate is centrifuged, and the objective antigen is separated from the obtained supernatant liquid. The concentration of the surface active agent is preferably about 0.2%. The content of the antibody relating to the adult T-cell leukemia can be determined with high sensitivity by the enzymatic immunoassay, radioimmunoassay or passive hemagglutination method using said antigen.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过在表面活性剂的存在下搅拌能够产生与成体T细胞白血病有关的抗原的细胞来制备标题抗原。构成：在表面活性剂（例如脱氧胆酸钠，辛基苯氧基聚乙氧基 - 乙醇等）的存在下，能够产生与成体T细胞白血病（例如MT-2细胞）相关的抗原的细胞被搅拌，将混合物离心，并用磷酸缓冲的生理盐水透析上清液。包含在上层液体中的表面活性剂可以通过透析除去。将透析液离心，将目的抗原与获得的上清液分离。表面活性剂的浓度优选为约0.2％。与成人T细胞白血病有关的抗体的含量可以通过使用所述抗原的酶免疫测定，放射免疫测定法或被动血凝法以高灵敏度来测定。

4. 发明专利

JPH0392760A METHOD AND REAGENT FOR MEASURING ANTIBODY EXCLUDING NONSPECIFIC ADSORPTION 失效
公开(公告)号：JPH0392760A
公开(公告)日：1991-04-17
申请号：JP22975389
申请日：1989-09-05
申请人： EISAI CO LTD
发明人： SAWADA TAKASHI , OBARA TAKASHI , MIYOSHI ISAO , TAGUCHI HIROKUNI
IPC分类号： G01N33/531 , G01N33/543 , G01N33/569 , G01N33/574
摘要： PURPOSE:To improve detecting sensitivity by performing addition of a serum sample under the specified conditions. CONSTITUTION:A serum sample is added in a solid phase, and the antibody in the sample is measured under the conditions of pH 9-10. For this purpose, acid or alkali is added, and the pH can be adjusted. Usually, however, the pH is adjusted by adding buffer solution. Any kind of buffer solution can be used as the kinds when the buffer solution has the buffer capacity at pH 9 - 10 as the kinds of the buffer solutions. For example, phosphoric acid buffer solution, tris hydrochloric acid buffer solution and the like can be used. The concentration of the buffer solution is about 0.05 - 0.01M. In this way, the nonspecific adsorption of the denaturated antibody in the sample serum which has been preserved for a long period or which is heated and inactivated is excluded. Thus, the detecting sensitivity can be improved.

5. 发明专利

JPH0231692A NOVEL MONOCLONAL ANTIBODY AND USE THEREOF 失效
公开(公告)号：JPH0231692A
公开(公告)日：1990-02-01
申请号：JP18257488
申请日：1988-07-21
申请人： EISAI CO LTD
发明人： TAKEUCHI TAMOTSU , KUBONISHI ICHIROU , MIYOSHI ISAO
IPC分类号： G01N33/574 , A61K39/42 , C12N5/10 , C12N5/16 , C12N15/02 , C12N15/06 , C12P21/08 , C12R1/91 , G01N33/577
摘要： PURPOSE:To enable to provide a method and a reagent for detecting human myeloid series tumor by using a monochlonal antibody prepared from a hybridoma produced by fusing a myeloma cell with the splenic cell of an animal immunized with a human myeloid series tumor cell. CONSTITUTION:PL-21 is inoculated in the abdominal cavity of BALB/C mouse twice at an internal of 1 week and in the vein thereof once and after 3 days the splenic cells of the mouse are taken out from the inoculated mouse and subsequently fused with a myeloma cell NS-1 to form an antibody production- positive hybridoma, followed by screening and cloning the hybridoma to provide a monoclone cell strain. It provides a monoclonal antibody to subject the cell strains to an in vitro culture wherein the cell strain is cultured in a RPMI-1640 medium supplemented with a fetal calf serum or to an in vivo culture wherein the cell strain is mutliplied in the abdominal cavity of the BALB/C mouse, separated and purified. An indirect fluorescent antibody method is utilized for the detection of human myeloid series tumor using the provided antibody. A detection reagent contains the above-mentioned monoclonal antibody as an essential component.

6. 发明专利

JPS6036967A METHOD AND REAGENT FOR MEASURING ATLV ANTIBODY 失效
公开(公告)号：JPS6036967A
公开(公告)日：1985-02-26
申请号：JP14487483
申请日：1983-08-10
申请人： EISAI CO LTD
发明人： SAWADA TAKASHI , MIYOSHI ISAO , TAGUCHI HIROKUNI
IPC分类号： G01N33/574 , A61K39/42 , G01N33/569
摘要： PURPOSE:To enable exact decision of the presence of an ATLV antibody by using the result obtd. by using the antigen obtd. by treating an ATLV negative cell strain and making measurement with respect to a sample as contrast and making decision by comparison. CONSTITUTION:The constituting elements for the entire part of a measuring system are a solid phase, an antigen associated with ATL, the antigen of this invention, the same antibody, antihuman IgG antibody, enzyme and substrate. The cup of a microtiter plate for enzyme immonoassay or glass beads may be used for the solid phase. The antigen associated with ATL and the antigen are respectively dissolved in a physiological buffer salt soln. of 0.15M phosphoric acid and the soln. is put into, for example, a polystyrene cup for enzyme immunoassay and is allowed to stand for one hour at

7. 发明专利

JPS59151885A Cell strain related to leukemia of t cell of adult 失效
标题翻译：细胞株与成年细胞的淋巴细胞相关
公开(公告)号：JPS59151885A
公开(公告)日：1984-08-30
申请号：JP2454883
申请日：1983-02-18
申请人： Eisai Co Ltd
发明人： MIYOSHI ISAO
IPC分类号： G01N33/50 , A61K39/21 , C07K16/30 , C12N5/00 , C12N5/10 , C12N15/02 , C12R1/91 , G01N33/569 , G01N33/574
CPC分类号： C07K16/3061 , A61K2039/505 , A61K2039/5152 , Y10S435/948
摘要： PURPOSE: To prepare an antibody related to leukemia of T cell of adult having immunologically high specificity, by using a cell strain obtained by blending a leukocyte of an animal other than human and monkey with a cell capable of producing an antigen related to leukemia of T cell of adult, followed by cultivating the mixture.
CONSTITUTION: In preparing a cell strain related to leukemia of T cell of adult useful for immunizing an animal other than human and monkey, a leukocyte of the animal is blended with a cell capable of producing an antigen related to leukemia of T cell of adult and cultivated. The cell strain related to leukemia of T cell of adult thus prepared is inoculated into the aminal to give an anti- serum related to leukemia of T cell of addult. The cell capable of producing an antigen related to leukemia of T cell of adult is preferably MT-2 cell, and rabbit may be used as the animal other than human and monkey.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：制备具有免疫学特异性的成人T细胞白血病抗体，通过使用将人和猴以外的动物的白细胞与能够产生与T型白血病有关的抗原的细胞混合获得的细胞株成年细胞，然后培养混合物。构成：在制备可用于免疫人和猴以外的动物的成人T细胞相关白血病细胞株时，将动物的白细胞与能够产生与成人T细胞白血病有关的抗原的细胞混合，以及栽培。将如此制备的成体的T细胞白血病相关细胞株接种到阿米尔氏液中，得到与添加的T细胞白血病有关的抗血清。能够产生与成人T细胞白血病相关的抗原的细胞优选为MT-2细胞，并且兔可以用作除人和猴以外的动物。

8. 发明专利

JPH10332688A SLIDE FOR SEPARATING CELL 失效
公开(公告)号：JPH10332688A
公开(公告)日：1998-12-18
申请号：JP14366997
申请日：1997-06-02
申请人： EISAI CO LTD
发明人： MIYAGI TATSUSHI , MIYOSHI ISAO , SAWADA TAKASHI
IPC分类号： G01N33/48 , G01N1/28
摘要： PROBLEM TO BE SOLVED: To separate a cell without requiring any skill by adhering the cell to a slide and cutting a part where at least one cell is adhered. SOLUTION: A cell is adhered to a slide, for example, by a method for dropping the cell floating liquid that is diluted to a proper concentration on the slide, and using a site spin and a method for applying the floating liquid. For example, the cell that is adhered is observed, for example, by a microscope, a target cell is selected out of a plurality of types of cells and the position is confirmed, that part is cut, for example, by a surgical knife, and a target cell is separated. The slide is preferably made of a transparent material to verify the adhesion position of the cell under the inspection by a microscope and is preferably made of a material that can be easily cut, for example, by the surgical knife, thus examining the presence or absence of contamination by a virus and variation of cancer genes and cancer inhibitors.

9. 发明专利

JPS60249058A METHOD AND REAGENT FOR MEASURING ATL VIRUS ANTIBODY 失效
公开(公告)号：JPS60249058A
公开(公告)日：1985-12-09
申请号：JP10447284
申请日：1984-05-25
申请人： EISAI CO LTD
发明人： FUJIMATSU JIYUNICHI , SAWADA TAKASHI , MIYOSHI ISAO , TAGUCHI HIROKUNI
IPC分类号： G01N33/574 , A61K39/00 , C12M1/40 , C12Q1/70 , G01N33/569
摘要： PURPOSE:To detect exactly the antibody in a sample to be inspected by an enzyme immunological measurement method, etc. in the stage of using an antigen associated to the adult T cell leukemia (ATL) to measure an ATL virus antibody by using the sample prepd. by adding the ATL virus antigen to the sample to be examined as a contrast sample. CONSTITUTION:The antigen associated to ATL obtd. by treating the MT-2 cell obtd. by the mixed culture with the leukemia cell sampled from the peripheral blood of an ATL patient with a surface active agent is conjugated at a substantial amt. to the inside surface of a cup for immunological measurement. A specified amt. of the ATL virus antigen obtd. from the MT- 2 cell cultured liquid and the serum to be inspected is added to one group (b) of the cup. Only the serum to be inspected is added to the other one group (a) and only the serum of the normal rabbit to be used for dilution of the serum to be inspected or the ATL virus antigen is added to another one group (c). After the respective groups are incubated, the ATL virus antibody labeled by enzyme, radioactivity, etc. is added to the cup to effect a reaction and then to effect the reaction of enzyme with a substrate. The coloration and radioactivity, etc. after specified time are measured and the coloration and radioactivity of the cup groups (a), (b), (c) with the substrate are measured. The rate of inhibition expressed by the formula is calculated from the measured values A, B, C thereof, and >=50% rate of inhibition is determined as antibody positive and

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