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    • 1. 发明专利
    • IgA ANTIBODY MEASURING METHOD
    • IgA抗体测量方法
    • JP2009031001A
    • 2009-02-12
    • JP2007192364
    • 2007-07-24
    • Dainichiseika Color & Chem Mfg Co LtdNational Institute Of Infectious Diseases国立感染症研究所長大日精化工業株式会社
    • KOBAYASHI TAKASHIYAMANAMI TAKANORITSUCHIDA SHINYAMIYAMURA TATSUOISHIKAWA KOICHI
    • G01N33/543G01N33/53
    • PROBLEM TO BE SOLVED: To provide a method for evaluating a generation degree of non-specific reaction with respect to a plate, after the blocking used in an ELISA method.
      SOLUTION: This method for evaluating the production degree of non-specific reaction in an ELISA plate includes a process for coating a part of the well of the ELISA plate with an antigen, a process for making a blocking agent act, the process for making immunoglobulin that specifically interacts with the antigen act, a process for making secondary antibody that specifically interacts with the immunoglobulin for act the process for making enzyme labeled tertiary antibody act, a process for adding the substrate of enzyme to subject it to enzymatic reaction, and a process for comparing the degree of the enzymatic reaction of the well coated with the antigen, with the degree of the enzymatic reaction of a well which is not coated with antigen.
      COPYRIGHT: (C)2009,JPO&INPIT
    • 待解决的问题:提供一种用于在ELISA法中使用的阻断后,评价非特异性反应生成度相对于板的方法。 解决方案:用于评价ELISA板中非特异性反应的生产程度的方法包括用抗原涂覆ELISA板的一部分孔的方法,制备阻断剂的方法,该方法 用于制备与抗原作用特异性相互作用的免疫球蛋白,制备与免疫球蛋白特异性相互作用的二抗的方法,用于制备酶标记的三级抗体作用的方法,将酶底物加入酶反应的方法, 以及用于比较涂覆有抗原的井的酶反应程度与未涂覆有抗原的孔的酶反应程度的方法。 版权所有(C)2009,JPO&INPIT