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    • 2. 发明专利
    • Highly accurate detection method of trichophyton
    • 三叉树高精度检测方法
    • JP2014113079A
    • 2014-06-26
    • JP2012268420
    • 2012-12-07
    • Mitsubishi Chemical Medience Corp三菱化学メディエンス株式会社
    • MIKAWA TAKASHIFUJIWARA ERIKOTOZAWA FUMIKO
    • C12N15/09C12Q1/04C12Q1/68
    • PROBLEM TO BE SOLVED: To provide a method of accurately and simply detecting a trichophyton bacteria in nail tinea and identifying a type thereof, and to provide a primer set and a kit used in the method.SOLUTION: By using a set of a primer including a specific base sequence which is specific to a trichophyton bacterium, and a primer of other specific base sequence, a trichophyton bacterium is detected accurately and simply without detecting a fungus other than a trichophyton bacterium which is reported to be detected from a nail, and by cutting off a gene fragment amplified by using the primer set of the specific sequence, by a specific restriction enzyme, whether the trichophyton bacterium is T.rubrum or T.mentagrophytes is identified and thus identifying a type of the trichophyton bacterium.
    • 要解决的问题:提供一种准确且简单地检测指甲癣菌中的毛发细菌并鉴定其类型的方法,并提供该方法中使用的引物组和试剂盒。方法:通过使用一组引物,包括 特异性碱基序列,特异性的毛细管细菌特异性碱基序列和其他特定碱基序列的引物,毛细管细菌被检测准确而简单,而不检测除了从钉子检测到的毛毛虫细菌以外的真菌, 通过特异性限制酶切断通过使用特异性序列的引物组扩增的基因片段,鉴定毛滴虫细菌是T.rubrum还是T.mentagrophytes,从而鉴定毛滴虫细菌的类型。