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1. 发明专利

JP2006071631A Detection method for virus in pestivirus of fravivirus, and use thereof in immuno-chromatography 审中-公开
标题翻译：番茄病毒的病毒检测方法及其在免疫色谱中的应用
公开(公告)号：JP2006071631A
公开(公告)日：2006-03-16
申请号：JP2005224860
申请日：2005-08-03
申请人： Bl:Kk , 株式会社ビーエル
发明人： KIDA HIROSHI , SAKOTA YOSHIHIRO , NANBA YASUHARU , MOCHIZUKI TAKESHI
IPC分类号： G01N33/569 , C12N15/09 , G01N33/48 , G01N33/543 , G01N33/577
CPC分类号： Y02A50/60
摘要： PROBLEM TO BE SOLVED: To provide a method and a measuring instrument detecting quickly and easily viruses over a wide range in Pestivirus of Fravivirus. SOLUTION: This method for detecting the virus in Pestivirus of Fravivirus comprises a sandwich type immunoassay using the first antibody and the second antibody to an NS3 protein of the virus in Pestivirus of Fravivirus, in particular, an immuno-chromatographic measuring method and an immuno-chromatographic test strip. Either or both of the first antibody and the second antibody are preferably monoclonal antibodies reacting with the NS3 protein both in an immunostaining method and a Western blotting method. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供在弗拉维瓦病毒的瘟病毒中广泛范围内快速和容易地检测病毒的方法和测量仪器。解决方案：用于检测弗拉维瓦病毒的瘟病毒的病毒的方法包括使用第一抗体和第二抗体对弗拉维瓦病毒的瘟病毒中的病毒的NS3蛋白进行夹心型免疫测定，特别是免疫色谱测量方法和免疫色谱测试条。第一抗体和第二抗体中的任一种或两者优选是在免疫染色方法和Western印迹法中与NS3蛋白反应的单克隆抗体。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2006067979A Immunity detecting method for influenza a type virus 审中-公开
标题翻译：流感病毒类型的免疫检测方法
公开(公告)号：JP2006067979A
公开(公告)日：2006-03-16
申请号：JP2004258880
申请日：2004-09-06
申请人： Bl:Kk , 株式会社ビーエル
发明人： KIDA HIROSHI , SAKOTA YOSHIHIRO , NANBA YASUHARU , MOCHIZUKI TAKESHI
IPC分类号： C12N15/09 , C07K14/11 , C07K16/10 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/02 , C12P21/08 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/577
摘要： PROBLEM TO BE SOLVED: To provide a method for quickly and easily detecting a wide range of influenza A type viruses, and to provide an apparatus for the same. SOLUTION: A recombinant NS1 protein and a monoclonal antibody to the same are obtained by cloning a fraction of a gene encoding NS1 protein which is a non structural protein common in various kinds of influenza A type viruses. This invention provides an immunoassay method using an antibody against the NS1 protein of the influenza A type viruses, especially a sandwich type immunoassay method using a first antibody and a second antibody against the NS1 protein, in particular, the invention provides an immunochromatography method and a testing split for immunochromatography. At least one of the first antibody and the second antibody is preferably the monoclonal-antibody. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种快速且容易地检测大范围的甲型流感病毒的方法，并提供用于其的装置。解决方案：通过克隆编码作为各种甲型流感病毒类型中常见的非结构蛋白质的NS1蛋白的基因的一部分获得重组NS1蛋白及其单克隆抗体。本发明提供了使用针对A型流感病毒的NS1蛋白的抗体的免疫测定方法，特别是使用第一抗体和针对NS1蛋白的第二抗体的夹心型免疫测定方法，特别是本发明提供免疫色谱法和用于免疫层析的检测。第一抗体和第二抗体中的至少一种优选为单克隆抗体。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2008058208A Immuno-chromatography method using single-chain variant-part antibody 审中-公开
标题翻译：使用单链变体部分抗体的免疫色谱法
公开(公告)号：JP2008058208A
公开(公告)日：2008-03-13
申请号：JP2006237181
申请日：2006-09-01
申请人： Bl:Kk , Shizuoka Prefecture , 株式会社ビーエル , 静岡県
发明人： OTA TOSHIYA , IIZUKA CHIKAYO , YAMADA SHINYA , MOCHIZUKI TAKESHI , NANBA YASUHARU
IPC分类号： G01N33/543 , C07K16/00 , C12N15/09 , C12P19/34 , C40B40/08 , G01N33/531
摘要： PROBLEM TO BE SOLVED: To provide a sandwich type immunoassay using recombination protein having a bonding property to an antigen, especially an immuno-chromatography method, and recombination protein suitable for use for this. SOLUTION: In the sandwich type immunoassay using a first bonding material and a second bonding material to antigen to be measured, at least one of the first bonding material and second bonding material is made of recombination protein having a heavy chain variant-part and light chain variant-part of the antibody to the antigen. Preferably, the recombination protein is single-chain variant-part fragment type antibody (scFv antibody) obtained by a phage displaying method. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：使用具有与抗原结合特性的重组蛋白质，特别是免疫色谱法，以及适用于此的重组蛋白质提供夹层型免疫测定法。解决方案：在使用第一接合材料和第二接合材料对要测量的抗原的夹心型免疫测定中，第一接合材料和第二接合材料中的至少一个由具有重链变体部分的重组蛋白质和轻链变体部分抗原的抗体。优选地，重组蛋白是通过噬菌体展示法获得的单链变体部分片段型抗体（scFv抗体）。版权所有（C）2008，JPO＆INPIT

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