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1. 发明专利

JP2012024096A Method for manufacturing dimethylglycine 有权
标题翻译：制造二甲基乙酰胺的方法
公开(公告)号：JP2012024096A
公开(公告)日：2012-02-09
申请号：JP2011201192
申请日：2011-09-14
申请人： Asahi Kasei Chemicals Corp , Kobe Univ , 国立大学法人神戸大学 , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , NAKAYAMA HIDEKI , KONDO AKIHIKO
IPC分类号： C12P13/04 , C12N9/00 , C12N9/10 , C12N15/09
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for more easily and effectively manufacturing dimethylglycine.SOLUTION: Dimethylglycine is manufactured by introducing exogenous genes related to a metabolic pathway for dimethylglycine synthesis by two-staged methylation reaction from glycine to sarcosine and from sarcosine to dimethylglycine into microorganisms. Further, by strenghthening a synthetic system of S-adenosylmethionine (SAM) to be used as a methyl group donor in the dimethylglycine synthesis at the same time, a larger amount of dimethylglycine can be biosynthesized. According to the method of manufacture, dimethylglycine can be highly densely accumulated inside the microorganism from a simple carbon source derived from plant biomass, and further, dimethylglycine can be easily separated and collected by discharging dimethylglycine under a low osmotic pressure condition such as fresh water. Further, by repeatedly utilizing the used microorganism, a large amount of dimethylglycine can be continuously produced.
摘要翻译：要解决的问题：提供一种更容易且有效地制备二甲基甘氨酸的方法。解决方案：通过从甘氨酸到肌氨酸和从肌氨酸到二甲基甘氨酸的两步甲基化反应引入与二甲基甘氨酸合成的代谢途径相关的外源基因来制备二甲基甘氨酸。此外，通过在二甲基甘氨酸合成中同时加强用作甲基供体的S-腺苷甲硫氨酸（SAM）的合成体系，可以生物合成更多量的二甲基甘氨酸。根据制造方法，二甲基甘氨酸可以从源自植物生物质的简单碳源高度密集地堆积在微生物中，而且在淡水等低渗透压条件下，二甲基甘氨酸可以容易地分离和收集二甲基甘氨酸。此外，通过反复利用所使用的微生物，可以连续生产大量的二甲基甘氨酸。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2012029679A Method for producing compatible solute 有权
标题翻译：生产兼容溶液的方法
公开(公告)号：JP2012029679A
公开(公告)日：2012-02-16
申请号：JP2011094455
申请日：2011-04-20
申请人： Asahi Kasei Chemicals Corp , Kobe Univ , 国立大学法人神戸大学 , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , NAKAYAMA HIDEKI , KONDO AKIHIKO
IPC分类号： C12P13/04 , C12N1/21 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for easily producing a desired compatible solute in large quantities, to be concrete, to provide a method in which the desired compatible solute is biosynthesized by microorganism and is easily separated and retrieved.SOLUTION: The production method includes: lowering or deleting the intrinsic ability of a microorganism to biosynthesize main compatible solutes; introducing into the microorganism exogenous genes relevant to biosynthesis of the desired compatible solute; culturing the microorganism under hyperosmotic conditions; and the process of biosynthesizing the desired compatible solute in the microorganism. The method further includes the process of allowing the desired compatible solute biosynthesized in the microorganism to be discharged and retrieved from the microorganism under hyposmotic conditions.
摘要翻译：要解决的问题：为了提供一种容易地产生所需的相容性溶质的方法，作为具体，可提供一种方法，其中所需的相容性溶质由微生物生物合成并易于分离和回收。解决方案：生产方法包括：降低或缺失微生物生物合成主要相容溶质的固有能力; 将与所需相容性溶质的生物合成相关的微生物外源基因导入微生物; 在高渗条件下培养微生物; 以及生物合成微生物中所需的相容性溶质的过程。该方法还包括使在微生物中生物合成的期望的相容性溶质在低渗条件下从微生物排出和回收的方法。版权所有（C）2012，JPO＆INPIT

3. 发明专利

JP2013042749A Food composition and pharmaceutical, containing planteose 有权
标题翻译：食品组合物和药物，包含植物
公开(公告)号：JP2013042749A
公开(公告)日：2013-03-04
申请号：JP2011185270
申请日：2011-08-26
申请人： Nippon Beet Sugar Mfg Co Ltd , 日本甜菜製糖株式会社 , Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： HORIUCHI KENICHI , NAGURA TAIZO , SAKURAI HIROAKI , NAKATA HIROAKI , YAMANAKA SAYAKA , KONISHI KAZUMASA , IMAZU SHINICHI
IPC分类号： A23L1/30 , A61K8/60 , A61Q11/00
摘要： PROBLEM TO BE SOLVED: To provide a food composition containing a low caloric functional saccharide raw material as an active ingredient and exhibiting useful physiological effects in human and animals other than the human, a method for producing the same, a useful pharmaceutical, etc.SOLUTION: This food composition capable of expecting an anti-dental caries effect and/or a bifidobacteria propagation effect is provided simply and efficiently by producing the food composition containing 1% or more of planteose, and also, the pharmaceutical exhibiting the anti-dental caries effect and/or bifidobacteria propagation effect is provided by incorporating the planteose as an active ingredient.
摘要翻译：待解决的问题：提供一种含有低热量功能性糖原料作为活性成分的食品组合物，并且对除人以外的人和动物表现出有用的生理作用，其制备方法，有用的药物，等等。解决方案：通过生产含有1％或更多种植物的食物组合物，简单有效地提供了能够预期抗龋齿效应和/或双歧杆菌繁殖效果的食品组合物，以及药物展示抗龋齿效果和/或双歧杆菌繁殖效果是通过将芸薹素作为活性成分并入来提供的。版权所有（C）2013，JPO＆INPIT

4. 发明专利

JP2010227023A Method for producing 2-deoxy-scyllo-inosose by nadh/nad ratio control 审中-公开
标题翻译：通过NADH / NAD比例控制生产2-脱氧肌氨酸的方法
公开(公告)号：JP2010227023A
公开(公告)日：2010-10-14
申请号：JP2009078714
申请日：2009-03-27
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , IMAZU SHINICHI
IPC分类号： C12P19/02 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide an intracellular metabolite control technique for dramatically improving 2-deoxy-scyllo-inosose (DOI) productivity.
SOLUTION: In the method for producing 2-deoxy-scyllo-inosose from glucose-6-phosphate by using a 2-deoxy-scyllo-inosose synthase or a microorganism capable of expressing a 2-deoxy-scyllo-inosose synthase, the ratio of NADH/NAD in the reaction system is controlled to be not more than 1.4.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供细胞内代谢物控制技术，以显着改善2-脱氧 - 青花肉苷（DOI）生产力。解决方案：通过使用2-脱氧 - 青蟹肌糖合成酶或能够表达2-脱氧十字糖 - 糖糖合成酶的微生物从葡萄糖-6-磷酸生产2-脱氧 - 青蟹肌糖的方法中，反应体系中NADH / NAD的比例控制在1.4以下。版权所有（C）2011，JPO＆INPIT

5. 发明专利

JP2014176390A Production method of myoinositol and myoinositol derivatives 有权
标题翻译： MYOINOSITOL和MYOINOSITOL衍生物的生产方法
公开(公告)号：JP2014176390A
公开(公告)日：2014-09-25
申请号：JP2014120340
申请日：2014-06-11
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , IMAZU SHINICHI
IPC分类号： C12N15/09 , C12N1/21 , C12P19/12
CPC分类号： C12P7/02 , C07H15/207 , C12N9/16 , C12N9/90 , C12N15/70 , C12P19/46 , C12Y301/03025 , C12Y505/01004 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide significantly improved myoinositol productivity to host microorganisms lacking an endogenous myoinositol biosynthetic pathway such as Escherichia coli or the like suitable for application of gene recombinant technology and synthetic biological methods.SOLUTION: In transformants obtained by introducing a myoinositol biosynthetic pathway into host microorganisms lacking an endogenous myoinositol biosynthetic pathway, inositol monophosphatase activity is strengthened.
摘要翻译：要解决的问题：提供显着改善的肌醇生产力来承载缺乏内源性肌醇生物合成途径的微生物，如适用于应用基因重组技术和合成生物学方法的大肠杆菌等。解决方案：在通过引入肌醇生物合成途径进入宿主微生物缺乏内源性肌醇生物合成途径，肌醇单磷酸酶活性得到加强。

6. 发明专利

JP2014057602A Production method of myoinositol and myoinositol derivatives 有权
公开(公告)号：JP2014057602A
公开(公告)日：2014-04-03
申请号：JP2013259477
申请日：2013-12-16
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , IMAZU SHINICHI , SATO MAYUMI
IPC分类号： C12P7/02 , C12N1/21 , C12N15/09
CPC分类号： C12P7/02 , C07H15/207 , C12N9/16 , C12N9/90 , C12N15/70 , C12P19/46 , C12Y301/03025 , C12Y505/01004 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide significantly improved myoinositol productivity to host microorganisms lacking an endogenous myoinositol biosynthetic pathway such as Escherichia coli or the like suitable for application of gene recombinant technology and synthetic biological methods.SOLUTION: In transformants obtained by introducing a myoinositol biosynthetic pathway into host microorganisms lacking an endogenous myoinositol biosynthetic pathway, inositol monophosphatase activity is strengthened.

7. 发明专利

JP2009183243A Method of producing melibiose 有权
标题翻译：生产胶原蛋白的方法
公开(公告)号：JP2009183243A
公开(公告)日：2009-08-20
申请号：JP2008028549
申请日：2008-02-08
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA
IPC分类号： C12P19/12
摘要： PROBLEM TO BE SOLVED: To provide a method of producing melibiose capable of selectively producing melibiose from glucose and galactose as inexpensive raw materials.
SOLUTION: The method of producing melibiose using glucose and galactose as raw materials, uses α-galactosidase derived from a stock belonging to Geobacillus thermocatenulatus which can selectively synthesize the melibiose.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种生产能够从葡萄糖和半乳糖选择性地生产蜜二糖的蜜二糖的廉价原料的生产方法。解决方案：使用葡萄糖和半乳糖作为原料生产蜜二糖的方法使用衍生自可以选择性合成蜜二糖的热带地热芽孢杆菌的原料的α-半乳糖苷酶。版权所有（C）2009，JPO＆INPIT

8. 发明专利

JP2009148189A NEW METHOD FOR PRODUCING α-GALACTO-OLIGOSACCHARIDE 有权
标题翻译：用于生产α-谷氨酸低聚糖的新方法
公开(公告)号：JP2009148189A
公开(公告)日：2009-07-09
申请号：JP2007328253
申请日：2007-12-20
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA
IPC分类号： C12P19/44 , C12N1/20
摘要： PROBLEM TO BE SOLVED: To provide a method for simply and inexpensively producing an α-galacto-oligosaccharide containing no foreign oligosaccharide except the α-galacto-oligosaccharide.
SOLUTION: The method for producing an α-galacto-oligosaccharide from a raw material containing sucrose and galactose comprises using an α-galactosidase derived from a strain belonging to Geobacillus thermocatenulatus.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种简单廉价地制造除了α-半乳寡糖以外不含外来寡糖的α-半乳寡糖的方法。解决方案：从含有蔗糖和半乳糖的原料制备α-半乳糖寡糖的方法包括使用衍生自热带地热芽孢杆菌菌株的α-半乳糖苷酶。版权所有（C）2009，JPO＆INPIT

9. 发明专利

JP2007215505A Cellulase, and method for producing cellooligosaccharide 审中-公开
标题翻译：纤维素和生产氯氰菊酯的方法
公开(公告)号：JP2007215505A
公开(公告)日：2007-08-30
申请号：JP2006041149
申请日：2006-02-17
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , ISAKA KOJI , YAMAZAKI YUSUKE
IPC分类号： C12N9/42 , C12P19/14 , C12R1/885
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an enzyme liquid, mass-securing a cultured supernatant enzyme liquid high in cellulase activity and high in cellooligosaccharide selectivity in selectively producing cellobiose in high yield by enzymolysis of a cellulosic material as feedstock in the presence of cellulase, and to provide a method for producing a cellooligosaccharide using the enzyme liquid. SOLUTION: The method for producing the cellulase-containing enzyme liquid comprises inoculating microorganisms belonging to the genus Trichoderma into a liquid medium containing 0.1-10 mass% of cottonseed meal followed by making a culture. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种酶液的制造方法，通过将纤维素材料作为原料进行酶解，以高产率选择性地高产率地选择性地生产纤维二糖，大量固定培养的上清液液体，纤维素酶活性高，纤维素糖选择性高在纤维素酶的存在下，提供使用酶液制造纤维寡糖的方法。解决方案：含有纤维素酶的酶液的制备方法包括将属于木霉属属的微生物接种到含有0.1-10质量％棉籽粉的液体培养基中，然后进行培养。版权所有（C）2007，JPO＆INPIT

10. 发明专利

JP2013135697A Novel 2-deoxy-scyllo-inosose synthase 有权
标题翻译：新型2-脱氧 - 氨基磺酸合成酶
公开(公告)号：JP2013135697A
公开(公告)日：2013-07-11
申请号：JP2013080207
申请日：2013-04-08
申请人： Asahi Kasei Chemicals Corp , 旭化成ケミカルズ株式会社
发明人： KONISHI KAZUMASA , IMAZU SHINICHI
IPC分类号： C12P7/26 , C12N15/09
CPC分类号： C12P7/26 , C12N9/93
摘要： PROBLEM TO BE SOLVED: To provide a DOI synthase having superior properties including stability to heat and pH compared to those of conventional enzymes and to provide a process for producing DOI using the enzyme.SOLUTION: There is provided the process for producing 2-deoxy-scyllo-inosose from glucose-6-phosphate by culturing microorganisms capable of expressing a 2-deoxy-scyllo-inosose synthase in a medium containing a cobalt ion in a range of 0.1 to 50 mg/L, relative to cobalt chloride hexahydrate.
摘要翻译：要解决的问题：提供具有优异性能的DOI合酶，其包括与常规酶的热稳定性和pH的稳定性，并提供使用该酶生产DOI的方法。解决方案：提供了生产2-脱氧 - 通过在相对于氯化钴六水合物的0.1〜50mg / L范围内培养含有钴离子的培养基中培养能够表达2-脱氧 - 青蟹肌糖合成酶的微生物，来自葡萄糖-6-磷酸的鲨肌蛋白。

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