专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明专利

JP2008278769A Method for producing three-dimensional aggregate comprising pancreatic islet cells in vitro 审中-公开
标题翻译：生产包含胰岛素分离细胞的三维聚集体的方法
公开(公告)号：JP2008278769A
公开(公告)日：2008-11-20
申请号：JP2007123708
申请日：2007-05-08
申请人： Hokkaido Univ , 国立大学法人北海道大学
发明人： TSUKIYAMA SHUSAKU , TANAKA MASARU , YAMAMOTO SADAAKI , SHIMOMURA MASATSUGU , MATSUSHITA MICHIAKI , TODO SHO
IPC分类号： A61L27/00 , C12N5/07 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide a method for culturing pancreatic islet cells in vitro for a long period without losing the specific functions. SOLUTION: This method for producing the three-dimensional aggregate comprising the pancreatic islet cells in vitro comprises a process (a) for preparing a honeycomb-like porous body comprising a water-insoluble polymer coated with an extracellular substrate, and a process (b) for culturing the isolated pancreatic islet cells on the honeycomb-like porous body. According to this production method, the three-dimensional aggregate can be formed without forming a single layer, even when the pancreatic islet cells are cultured in vitro. Therefore, the pancreatic islet cell aggregate can be produced and collected without using a protein digestion enzyme such as trypsin, while maintaining the functions of pancreatic islet cells, such as an insulin-secreting ability. Such the pancreatic islet cells are useful for pancreatic islet transplantation treatments. The honeycomb-like porous body holding the three-dimensional aggregate comprising the pancreatic islet cells can be used as bio-artificial pancreas, cell chips or a carrier for transplanting pancreatic islet cells. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种长期培养胰岛细胞的方法，而不失去特定功能。解决方案：用于生产包含胰岛细胞的三维骨料的方法在体外包括制备蜂窝状多孔体的方法（a），该蜂窝状多孔体包含涂覆有细胞外基质的水不溶性聚合物，以及方法（b）在蜂窝状多孔体上培养分离的胰岛细胞。根据该制造方法，即使在体外培养胰岛细胞的情况下，也可以形成三维聚集体而不形成单层。因此，可以在不使用蛋白质消化酶如胰蛋白酶的情况下生产和收集胰岛细胞聚集体，同时保持胰岛细胞的功能，例如胰岛素分泌能力。这种胰岛细胞可用于胰岛移植治疗。保持包含胰岛细胞的三维聚集体的蜂窝状多孔体可以用作生物人造胰腺，细胞碎片或用于移植胰岛细胞的载体。版权所有（C）2009，JPO＆INPIT

IPRDB

热门服务

关于我们

友情链接

联系方式