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1. 发明专利

JP2012047760A Improved flow cytometer nozzle and flow cytometer sample handling method 有权
标题翻译：改进的流量测量仪喷嘴和流量测量仪样品处理方法
公开(公告)号：JP2012047760A
公开(公告)日：2012-03-08
申请号：JP2011265651
申请日：2011-12-05
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： CHRISTOPHER S BUCHANAN , HERICKHOFF LISA
IPC分类号： A01K67/02 , G01N15/14 , A61D19/00 , C12M1/00 , C12N5/076 , C12Q1/02 , G01N1/00 , G01N15/00
CPC分类号： G01N15/1404 , G01N2015/0065 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413 , G01N2015/1415 , G01N2015/149 , Y10T436/101666
摘要： PROBLEM TO BE SOLVED: To provide an improved nozzle device for flow cytometer systems, and a method for improving cytometry.SOLUTION: There is provided an improved nozzle interior surface geometry that produces the simplest flow path for applying necessary hydrodynamic force to accelerate and perhaps orient a sample into a proper direction for analyzing and efficient sorting purposes. This improved nozzle interior surface geometry comprise: either or both of an appropriately configured accelerative force feature and/or an elliptical-like, single torsional interior surface element within a single torsional orientation nozzle that produces special hydrodynamic force, namely, single torsional orientation force.
摘要翻译：要解决的问题：提供用于流式细胞仪系统的改进的喷嘴装置和改进细胞计数的方法。解决方案：提供了改进的喷嘴内表面几何形状，其产生最简单的流动路径，用于施加必要的流体动力以加速并且可能将样品定向到适当的方向以进行分析和有效的分选目的。这种改进的喷嘴内表面几何形状包括：在单个扭转定向喷嘴内的适当配置的加速力特征和/或椭圆形单个扭转内表面元件中的一个或两者，其产生特殊的流体动力，即单个扭转取向力。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2013066491A Method and apparatus for reducing protein content in sperm cell extender 审中-公开
标题翻译：用于减少细胞扩增器中蛋白质含量的方法和装置
公开(公告)号：JP2013066491A
公开(公告)日：2013-04-18
申请号：JP2013008137
申请日：2013-01-21
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： SCHENK JOHN L
IPC分类号： C12N5/076 , C12N1/04
CPC分类号： A01N1/02 , A01N1/0221
摘要： PROBLEM TO BE SOLVED: To provide methods and an apparatus for reducing protein content in sperm cell extenders.SOLUTION: The inventive technology relates to a method and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing new values of reduced protein content in sperm cell extenders.
摘要翻译：要解决的问题：提供减少精子细胞扩增剂中的蛋白质含量的方法和装置。解决方案：本发明技术涉及一种用于减少精子细胞扩增剂中的蛋白质含量的方法和装置，并且可以包括以下特征中的一个或多个：用于减少精子细胞增量剂中的蛋白质含量的技术; 用于降低含有冷冻保护剂的B部分精子细胞延长剂中的蛋白质含量的技术; 制备不需要澄清的精子细胞扩增剂的技术; 制备适合离心的低密度梯度精子细胞增量剂的技术; 用于在制备精子细胞延长剂的各个步骤之间降低蛋白质含量的技术，以及用于在精子细胞扩增剂中建立蛋白质含量降低的新值的技术。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2011250793A Sperm cell process system 审中-公开
标题翻译： SPERM细胞过程系统
公开(公告)号：JP2011250793A
公开(公告)日：2011-12-15
申请号：JP2011164799
申请日：2011-07-27
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： LINDSEY ALLISON C , SCHENK JOHN L
IPC分类号： A01K67/02 , C12N5/076 , A01N1/02 , A61K35/52 , B01L20060101 , C12N1/02 , C12N5/00 , C12N5/071
CPC分类号： C12N5/0612 , A01N1/021 , A01N1/0284 , A61K35/52
摘要： PROBLEM TO BE SOLVED: To provide devices or methods for the collection, handling, transportation, storage, or separation of semen or sperm cells to maintain or increase sperm viability.SOLUTION: A semen or sperm cell process system is for maintaining or enhancing the biological, chemical, physical, physiological, or functional attributes of sperm cells within the context of various collecting, handling, storage, transportation, separation, or insemination procedures. A method for separating sperm cells includes steps of: obtaining semen from a male of a species of mammal; incubating the semen at a temperature above which sperm cell membrane lipids transit from a liquid phase to gel phase; determining a sperm cell characteristic of a plurality of the sperm cells; separating the sperm cells based upon the sperm cell characters; and collecting separated sperm cells.
摘要翻译：要解决的问题：提供用于收集，处理，运输，储存或分离精液或精子细胞以维持或增加精子活力的装置或方法。解决方案：精液或精子细胞过程系统用于在各种收集，处理，储存，运输，分离或授精过程的背景下维持或增强精子细胞的生物，化学，物理，生理或功能属性。分离精子细胞的方法包括以下步骤：从哺乳动物的雄性获得精液; 在精液细胞膜脂质从液相转移到凝胶相的温度下孵育精液; 确定多个精子细胞的精子细胞特征; 基于精子细胞特征分离精子细胞; 并收集分离的精子细胞。版权所有（C）2012，JPO＆INPIT

4. 发明专利

JP2014112108A Optical apparatus 有权
标题翻译：光学装置
公开(公告)号：JP2014112108A
公开(公告)日：2014-06-19
申请号：JP2014042303
申请日：2014-03-05
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： JONATHAN SHARP , SCHAARE PETER NIVEN
IPC分类号： G01N15/14 , G01N15/02 , G01N21/47 , G01N21/64 , G02B5/00 , G02B5/04 , G02B27/09
CPC分类号： G01N15/1436 , G01N15/1434 , G01N15/1459 , G01N21/49 , G01N2015/1452 , G01N2015/149 , G01N2021/6469 , G02B5/04
摘要： PROBLEM TO BE SOLVED: To provide an optical apparatus capable of providing a beam with a constant cross section from a single beam.SOLUTION: An optical apparatus comprises: a prism having a conical portion comprising an apex at a forward end of the prism and a central axis extending through the apex of the prism; an optical arrangement comprising a light source of electromagnetic radiation, and being adapted to direct an incident beam of the electromagnetic radiation to the apex of the conical portion in a direction substantially aligned with the central axis of the conical portion; and a reflective surface formed behind the apex of the prism. In the optical apparatus, the beam refracted by the prism is reflected by the reflective surface back through the prism to be projected from the forward end of the prism as an annular beam of the electromagnetic radiation.
摘要翻译：要解决的问题：提供一种能够从单个光束提供具有恒定横截面的光束的光学装置。解决方案：光学装置包括：棱镜，其具有锥形部分，该锥形部分包括在棱镜的前端处的顶点和中心轴延伸穿过棱镜的顶点; 光学装置，其包括电磁辐射光源，并且适于将电磁辐射的入射光束以与锥形部分的中心轴线基本对准的方向引导到圆锥形部分的顶点; 以及形成在棱镜的顶点后面的反射表面。在光学装置中，由棱镜折射的光束被反射面反射通过棱镜，从棱镜的前端作为电磁辐射的环形光束突出。

5. 发明专利

JP2010252801A Sex-specific insemination of mammals with low number of sperm cells 审中-公开
标题翻译：具有少量SPERM细胞的雄性特异性免疫球蛋白
公开(公告)号：JP2010252801A
公开(公告)日：2010-11-11
申请号：JP2010139978
申请日：2010-06-18
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： SEIDEL GEORGE E , HERICHOFF LISA , SCHENK JOHN
IPC分类号： A01K67/02 , A01K67/027 , A61D19/02 , A61D19/04 , C12N5/00 , C12N5/076 , C12N15/873 , G01N15/14
CPC分类号： C12N5/0612 , A01K67/027 , A61D19/02 , C12N15/873 , G01N2015/149
摘要： PROBLEM TO BE SOLVED: To achieve sexed insemination with lower dosages in a manner which works under realistic commercial circumstances. SOLUTION: A method of producing a mammal having a predetermined sex comprising the steps of: a. collecting sperm cells from a male species of a mammal; b. determining the sex characteristic of a plurality of the sperm cells; c. sorting the sperm cells according to the determination of their sex characteristic; d. establishing a sorted artificial insemination sample having about 10% to about 50% of the number of the sperm cells relative to a typical unsorted artificial insemination sample; e. inserting the sorted artificial insemination sample into a female species of the mammal; f. fertilizing at least one egg within the female species of the mammal at success levels (p>0.1) statistically comparable to the typical unsorted artificial insemination dosage; and g. producing an offspring mammal of the desired sex. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：以实际商业环境下工作的方式，以较低剂量实现性别授粉。解决方案：一种制备具有预定性别的哺乳动物的方法，包括以下步骤：a。从哺乳动物的雄性物种中收集精子细胞; 湾确定多个精子细胞的性别特征; C。根据其性别的确定对精子细胞进行分选; 天。建立相对于典型的未分类的人工授精样品，具有约10％至约50％的精子细胞的分选人工授精样品; 即将分选的人工授精样品插入哺乳动物的雌性物种中; F。在成功水平（p> 0.1）下，在哺乳动物的雌性物种内至少施用一个卵，与典型的未分类的人工授精剂量统计学相似; 和g。产生所需性别的后代哺乳动物。版权所有（C）2011，JPO＆INPIT

6. 发明专利

JP2014079259A High purity x-chromosome bearing populations of spermatozoa and y-chromosome bearing populations of spermatozoa 审中-公开
标题翻译： SPERMATOZOA和Y型染色体轴承人群的高纯X染色体轴承人群
公开(公告)号：JP2014079259A
公开(公告)日：2014-05-08
申请号：JP2014022282
申请日：2014-02-07
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： EVANS KENNETH M , VAN MUNSTER ERIK B
IPC分类号： C12M1/34 , G01N33/50 , A01N1/02 , A61D19/00 , C12M3/00 , C12N20060101 , C12N5/00 , C12N5/071 , C12N5/076 , C12N15/54 , C12Q1/02 , C12Q1/68 , G01N15/14 , G01N21/17 , G01N33/487
CPC分类号： C12Q1/6879 , A61D19/00 , B03B9/00 , C12N5/061 , C12N5/0612 , G01F17/00 , G01N15/14 , G01N15/147 , G01N21/17 , G01N2015/149
摘要： PROBLEM TO BE SOLVED: To provide isolated high-purity X-chromosome bearing populations of spermatozoa and Y-chromosome bearing populations of spermatozoa.SOLUTION: There are provided: isolated non-naturally occurring populations of spermatozoa (15) having high purity; and technologies to differentiate sperm (28) based on characteristics such as mass, volume, orientation, or emitted light, including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32). The isolated non-naturally occurring populations of spermatozoa having high purity have numerous applications including sex selection of offspring from mammals, various in vitro protocols for the fertilization of ova, various in vivo protocols such as artificial insemination, business methods involving the production of prize animals or meat animals, or preservation of rare or endangered animals, to recite but a few of the applications for high purity populations of spermatozoa.
摘要翻译：要解决的问题：提供分离的高纯度X染色体带有精子和Y染色体的精子数量的群体。解决方案：提供：具有高纯度的分离的非天然存在的精子（15）群体; 以及基于诸如质量，体积，取向或发射光的特性来区分精子（28）的技术，包括分析方法和诸如光束成形光学器件（30）和检测器（32）的装置。孤立的非天然存在的高纯度精子种群有许多应用，包括哺乳动物后代的性别选择，卵的受精的各种体外方案，各种体内方案如人工授精，涉及生产奖品的商业方法或肉类动物，或保存稀有或濒危动物，仅列举高纯度精子数量的一些应用。

7. 发明专利

JP2013078346A Sex-specific insemination of mammal with low number of sperm cell 审中-公开
标题翻译：具有低细胞数的MAMMAL特异性免疫球蛋白
公开(公告)号：JP2013078346A
公开(公告)日：2013-05-02
申请号：JP2013021330
申请日：2013-02-06
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： SEIDEL GEORGE E , HERICHOFF LISA , SCHENK JOHN
IPC分类号： A01K67/02 , A01K67/027 , A61D19/02 , A61D19/04 , C12N5/00 , C12N5/076 , C12N15/873 , G01N15/14
CPC分类号： C12N5/0612 , A01K67/027 , A61D19/02 , C12N15/873 , G01N2015/149
摘要： PROBLEM TO BE SOLVED: To achieve sexed insemination with lower dosages in a manner which works under realistic commercial circumstances.SOLUTION: A method of producing a mammal having a predetermined sex comprises the steps of: a. collecting sperm cells from a male species of a mammal; b. determining the sex characteristic of a plurality of the sperm cells; c. sorting the sperm cells according to the determination of their sex characteristic; d. establishing a sorted artificial insemination sample having about 10% to about 50% of the number of the sperm cells relative to a typical unsorted artificial insemination sample; e. inserting the sorted artificial insemination sample into a female species of the mammal; f. fertilizing at least one egg within the female species of the mammal at success levels (p>0.1) statistically comparable to the typical unsorted artificial insemination dosage; and g. producing an offspring mammal of the desired sex.
摘要翻译：要解决的问题：以实际商业环境下工作的方式，以较低剂量实现性别授粉。解决方案：制备具有预定性别的哺乳动物的方法包括以下步骤：a。从哺乳动物的雄性物种中收集精子细胞; b。确定多个精子细胞的性别特征; C。根据其性别的确定对精子细胞进行分选; d。建立相对于典型的未分类的人工授精样品，具有约10％至约50％的精子细胞的分选人工授精样品; e。将分选的人工授精样品插入哺乳动物的雌性物种中; F。在成功水平（p> 0.1）下，在哺乳动物的雌性物种内至少施用一个卵，与典型的未分类的人工授精剂量统计学相似; 和g。产生所需性别的后代哺乳动物。版权所有（C）2013，JPO＆INPIT

8. 发明专利

JP2012005490A High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa 审中-公开
标题翻译：高品质X染色体轴承和Y型染色体轴承人
公开(公告)号：JP2012005490A
公开(公告)日：2012-01-12
申请号：JP2011166045
申请日：2011-07-28
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： EVANS KENNETH M , VAN MUNSTER ERIK B
IPC分类号： C12N5/076 , G01N33/50 , A01N1/02 , A61D19/00 , C12M1/00 , C12M3/00 , C12N20060101 , C12N5/00 , C12N5/071 , C12N15/54 , C12Q1/02 , C12Q1/68 , G01N15/14 , G01N21/17 , G01N21/64 , G01N33/48 , G01N33/487
CPC分类号： C12Q1/6879 , A61D19/00 , B03B9/00 , C12N5/061 , C12N5/0612 , G01F17/00 , G01N15/14 , G01N15/147 , G01N21/17 , G01N2015/149
摘要： PROBLEM TO BE SOLVED: To provide isolated high-purity X-chromosome bearing or Y-chromosome bearing populations of spermatozoa.SOLUTION: There are provided: isolated non-naturally occurring populations of spermatozoa (15) having high purity; and technologies to differentiate sperm (28) based on characteristics such as mass, volume, orientation, or emitted light, including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32). The isolated non-naturally occurring populations of spermatozoa having high purity have numerous applications including sex selection of offspring from mammals, various in vitro protocols for the fertilization of ova, various in vivo protocols such as artificial insemination, business methods involving the production of prize animals or meat animals, or preservation of rare or endangered animals, to recite but a few of the applications for high purity populations of spermatozoa.
摘要翻译：要解决的问题：提供分离的高纯度X染色体携带或携带Y染色体的精子数量。解决方案：提供：具有高纯度的分离的非天然存在的精子群体（15）以及基于诸如质量，体积，取向或发射光的特性来区分精子（28）的技术，包括分析方法和诸如光束成形光学器件（30）和检测器（32）的装置。孤立的非天然存在的高纯度精子种群有许多应用，包括哺乳动物后代的性别选择，卵的受精的各种体外方案，各种体内方案如人工授精，涉及生产奖品的商业方法或肉类动物，或保存稀有或濒危动物，仅列举高纯度精子数量的一些应用。版权所有（C）2012，JPO＆INPIT

9. 发明专利

JP2011180144A Optical apparatus 有权
标题翻译：光学装置
公开(公告)号：JP2011180144A
公开(公告)日：2011-09-15
申请号：JP2011058447
申请日：2011-03-16
申请人： Xy Llc , エックスワイ,エルエルシー
发明人： SHARP JONATHAN , SCHAARE PETER NIVEN
IPC分类号： G01N15/14 , C12M1/00 , C12M1/34 , G01N15/02 , G01N21/47 , G01N21/64 , G02B5/00 , G02B5/04 , G02B5/10 , G02B27/09
CPC分类号： G01N15/1436 , G01N15/1434 , G01N15/1459 , G01N21/49 , G01N2015/1452 , G01N2015/149 , G01N2021/6469 , G02B5/04
摘要： PROBLEM TO BE SOLVED: To provide an optical apparatus which can provide a beam with a constant cross section from a single beam. SOLUTION: The optical apparatus includes: a prism having a conical portion with an apex at a forward end of the prism and a central axis extending through the apex of the prism; an optical arrangement which includes a source of electromagnetic radiation, and is adapted to direct an incident beam of electromagnetic radiation to the apex of the conical portion in a direction substantially aligned with the central axis of the conical portion; and a reflective surface formed behind the apex of the prism. In the optical apparatus, the beam refracted by the prism is reflected by the reflective surface back through the prism to be projected from the forward end of the prism as an annular beam of electromagnetic radiation. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种能够从单个波束提供具有恒定横截面的波束的光学装置。光学装置包括：具有锥形部分的棱镜，棱锥部分在棱镜的前端具有顶点，并且中心轴线延伸穿过棱镜的顶点; 光学装置，其包括电磁辐射源，并且适于将入射的电磁辐射束以与锥形部分的中心轴线基本对准的方向引导到锥形部分的顶点; 以及形成在棱镜的顶点后面的反射表面。在光学装置中，由棱镜折射的光束被反射表面反射回棱镜，从棱镜的前端作为环形的电磁辐射束突出。版权所有（C）2011，JPO＆INPIT

10. 发明专利

JP2010213719A Low pressure sperm cell separation system 审中-公开
标题翻译：低压SPELL细胞分离系统
公开(公告)号：JP2010213719A
公开(公告)日：2010-09-30
申请号：JP2010117830
申请日：2010-05-21
申请人： Colorado State Univ , Xy Llc , エックスワイ,エルエルシー , コロラドステートユニバーシティ
发明人： SCHENK JOHN L , SEIDEL GEORGE E , SUH TAE KWANG
IPC分类号： C12N5/076 , A01K67/02 , C12N5/00 , C12Q1/02
CPC分类号： C12N5/0612 , A01K67/027 , A01K2227/10 , A01K2227/101 , A61D19/00 , C12N5/061 , C12N2517/10 , Y10T442/2115
摘要： PROBLEM TO BE SOLVED: To provide a sperm cell process system providing to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells. SOLUTION: There is provided sperm cell process system to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells. There is provided a method of generating a sperm cell insemination sample, comprising: (a) obtaining semen from a male of a species of mammal; (b) generating a fluid stream having flow characteristics; (c) altering the flow characteristics of the fluid stream to adjust the fluid stream pressure; (d) entraining the sperm cells into the fluid stream; (e) controlling the sperm cell fertility characteristics through adjustment of the fluid stream pressure; and (f) generating a sperm cell insemination sample having controlled sperm cell fertility characteristics. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供精子细胞过程系统，提供精子细胞授精样品，其具有精子细胞的受精精子细胞生育特性。解决方案：提供了精子细胞过程系统来产生精子细胞授精样品，其具有精子细胞受精精子细胞生殖力特征。提供了一种产生精子细胞授精样品的方法，包括：（a）从哺乳动物的雄性获得精液; （b）产生具有流动特性的流体流; （c）改变流体流的流动特性以调节流体流压力; （d）将精子细胞吸入流体流中; （e）通过调节流体流压来控制精子细胞的生育特征; 和（f）产生具有受控精子细胞生育特征的精子细胞授精样品。版权所有（C）2010，JPO＆INPIT

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