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    • 1. 发明专利
    • JPS6111594B2 -
    • JPS6111594B2
    • 1986-04-03
    • JP17134781
    • 1981-10-28
    • Mitsuru Furusawa
    • FURUSAWA MITSURUYAMAMOTO FUMIICHIRO
    • C12N15/09C12M1/00C12N15/89
    • C12N15/89
    • PURPOSE:To inject DNA securely into cell and to carry out the titled transformation, by suspending an eucaryote in a DNA-containing solution, pricking the cell nucleus with a spicule from the outside, pulling it out. CONSTITUTION:An eucaryote (any of yeast, a fungus such as a mold, animal and plant, and, when one such as plant has a cell wall, the cell wall may be removed and the cell may be processed into protoplast) is placed on the Petri dish 1, a DNA-containing solution having the desired genetic information is added to the dish, the Petri dish 1 is placed on the objective 2, the central part of the cell nucleus is pricked through with the hypodermic needle 3, and it is pulled out, so that DNA is introduced into the cell. DNA is incorporated into chromosome without being modified at all. Consequently, almost in all cases, genetic information except that of injected DNA is not admixtured, and screening of genetic variant is not required. Useful for breeding of animal or plant, improvement of kinds, remedy for animal, etc.
    • 2. 发明专利
    • Method and system for rapidly conferring desired trait to organism
    • 方便和系统快速将所要求的旅行转交给有机体
    • JP2007143562A
    • 2007-06-14
    • JP2007059288
    • 2007-03-08
    • Mitsuru FurusawaNeo-Morgan Laboratory Inc古澤 満株式会社ネオ・モルガン研究所
    • FURUSAWA MITSURU
    • C12N15/09
    • PROBLEM TO BE SOLVED: To provide a method for conferring a desired hereditary trait to an organism in compliance with natural evolution. SOLUTION: A method is provided for regulating the conversion rate of a hereditary trait of a cell, comprises the step of (a) regulating the error-prone frequency of gene replication of the cell. A set of at least two kinds of polymerases is provided for use in regulating a conversion rate of a hereditary trait of an organism, wherein error-prone frequencies of the polymerases are different from each other. In one embodiment of the present invention, one of the at least two kinds of polymerases is involved in an error-prone frequency of a lagging strand, and another of the at least two kinds of polymerases is involved in an error-prone frequency of a leading strand. COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供一种根据自然进化赋予生物体所需的遗传性状的方法。 解决方案:提供一种用于调节细胞的遗传性状的转化率的方法,包括以下步骤:(a)调节细胞基因复制的易错频率。 提供一组至少两种聚合酶用于调节生物体的遗传性状的转化率,其中聚合酶的易错频率彼此不同。 在本发明的一个实施方案中,所述至少两种聚合酶中的一种聚合酶涉及滞后链的错误频率,并且所述至少两种聚合酶中的另一种涉及易于发生频率的 领先股。 版权所有(C)2007,JPO&INPIT