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1. 发明专利

JP4210769B2 A method for manufacturing a Gateway entry clone 有权
公开(公告)号：JP4210769B2
公开(公告)日：2009-01-21
申请号：JP2001357821
申请日：2001-11-22
申请人：インビトロジェン株式会社 , 独立行政法人産業技術総合研究所
发明人：直樹五島 , 佐紀園 , 康智木須 , 信夫野村
IPC分类号： C12N15/09 , C12N15/10 , C12N15/66 , C12Q1/68
CPC分类号： C12N15/102 , C12N15/66
摘要： The present invention provides materials and methods for the rapid cloning and mutagenesis of nucleic acid molecules. The present invention permits simultaneous introduction of a one or more point mutations and adapter sequences into a nucleic acid of interest.

2. 发明专利

JP2010248084A Method of synthesizing oligonucleotide using novel cleaning solvent 审中-公开
标题翻译：使用新型清洁溶剂合成寡核苷酸的方法
公开(公告)号：JP2010248084A
公开(公告)日：2010-11-04
申请号：JP2009096251
申请日：2009-04-10
申请人： Invitrogen Japan Kk , インビトロジェン株式会社
发明人： UTSUMI JUNO , YAMAKAGE SHUNICHI
IPC分类号： C07H21/04 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method of synthesizing an oligonucleotide using an alternative cleaning solvent.
SOLUTION: The method of chemically synthesizing an oligonucleotide in a solid phase according to a phosphoroamidite process is characterized by cleaning a solid state support using acetone or a liquid mixture of acetonitrile and acetone as a cleaning solvent upon completing each of its reaction processes.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供使用替代的清洁溶剂合成寡核苷酸的方法。解决方案：根据亚磷酰胺法在固相中化学合成寡核苷酸的方法的特征在于，当完成其每个反应过程时，使用丙酮或乙腈和丙酮的液体混合物作为清洁溶剂清洗固态载体。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2006141320A Method for cloning plural nucleic acid fragments 审中-公开
标题翻译：克隆平板核酸片段的方法
公开(公告)号：JP2006141320A
公开(公告)日：2006-06-08
申请号：JP2004337985
申请日：2004-11-22
申请人： Fumio Imamoto , Invitrogen Japan Kk , インビトロジェン株式会社 , 文男今本
发明人： IMAMOTO FUMIO , SONE TAKESHI
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a vector for loading plural kinds of nucleic acid fragments, efficiently. SOLUTION: This modular destination vector containing one nucleic acid fragment and one cassette is constructed by incubating a donor vector containing two or more nucleic acid fragments adjacent to two recombinant sites and a cassette adjacent to the two recombinant sites with a site specific recombinant protein for exchanging one of the nucleic acid fragments adjacent to the two recombinant sites contained in an entry vector for the cassette adjacent to the two recombinant sites contained in the donor vector. By incubating the modular destination vector with an entry vector containing two nucleic acid fragments adjacent to the two recombinant sites and the site specific recombinant protein for exchanging the cassette contained in the modular destination vector with the two nucleic acid fragments adjacent to the two recombinant sites contained in the entry vector, a vector containing three nucleic acid fragments is constructed. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种用于生产用于加载多种核酸片段的载体的方法。解决方案：含有一个核酸片段和一个盒的模块化目的载体通过将含有两个或更多个核酸片段的供体载体与两个重组位点相邻的构建体和与两个重组位点相邻的盒与位点特异重组体用于交换与供体载体中包含的两个重组位点相邻的盒的入口载体中包含的两个重组位点相邻的核酸片段之一的蛋白质。通过将模块化目的载体与包含与两个重组位点相邻的两个核酸片段的入口载体和用于将包含在模块化目的载体中的盒与包含两个重组位点的两个核酸片段交换的位点特异性重组蛋白进行孵育在入口载体中，构建含有三个核酸片段的载体。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2005151846A Method for making gateway entry clone 审中-公开
标题翻译：制作网关进入克隆的方法
公开(公告)号：JP2005151846A
公开(公告)日：2005-06-16
申请号：JP2003392942
申请日：2003-11-21
申请人： Invitrogen Japan Kk , インビトロジェン株式会社
发明人： TANAKA SHIGEO , KENMOCHI SEIWA
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a technique for the Gateway cloning of a large-size gene for which a PCR amplification becomes difficult and an error probability in the amplification becomes higher and for a cloning by introducing mutations of point mutation, deletion, insertion and substitution into a gene in high accuracy and efficiency. SOLUTION: A method for making a Gateway entry clone, comprises the following procedure: amplifying an N-terminal fragment using a primer A whose 5'-terminal is allowed to be added with a Gateway recombinant sequence attB1 and a primer D designed in a gene by PCR or adaptor PCR, and amplifying a C-terminated fragment using a primer C designed in contact with the primer D and a primer B to which a Gateway recombinant sequence attB2 can be added, thereby the C-terminal fragments and/or N-terminal fragments are integrated in an attP plasmid vector followed by cyclization by self ligation, and the cyclized product is then introduced into a competent cell. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：为了提供用于克隆扩增难以进行扩增的大尺寸基因的门户克隆技术，扩增中的错误概率变得更高，并且通过引入点突变的突变进行克隆，缺失，以高精度和高效率插入和取代基因。解决方案：用于制作网关入口克隆的方法包括以下步骤：使用引物A扩增N末端片段，引物A的5'末端允许加入Gateway重组序列attB1和设计的引物D 在通过PCR或衔接PCR的基因中，并使用与引物D接触设计的引物C和可以加入Gateway重组序列attB2的引物B扩增C-端片段，由此将C末端片段和/ 或N末端片段整合到attP质粒载体中，然后通过自连接进行环化，然后将环化的产物引入感受态细胞。版权所有（C）2005，JPO＆NCIPI

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