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    • 83. 发明专利
    • CARCINOSTATIC AGENT
    • JPS6219528A
    • 1987-01-28
    • JP15651785
    • 1985-07-16
    • CHLORELLA IND
    • SHINPO KUNIHIRO
    • A61K36/05A61K36/02A61K38/00A61P35/00C07K14/405C12P1/00
    • NEW MATERIAL:A glycoprotein having carcinostatic activity and the following characteristics. Molecular weight, 45,000; protein constitution, composed of alpha-helix and random coil; sugar constitution, composed of beta-polysaccharide; circular dichroic spectrum, having positive peak at 275nm. USE:Carbinostatic agent. PREPARATION:Chlorella (microbial green algae) is extracted with hot water, concentrated at 50 deg.C under reduced pressure, diluted with distilled water, and passed through a semi-permeable membrane to obtain a polymeric fraction which cannot be passed through the membrane. The fraction is adjusted to 5.5pH and hydrolyzed by the addition of a liquid containing alpha-amylase. The hydrolyzed liquid is heated at 80 deg.C to deactivate alpha-amylase, the whole solution is dialyzed, the hydrolyzed sugar is discharged, and the residue is dried under reduced pressure. The dried component is subjected to gel-filtration to obtain the objective glycoprotein component.