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51. 发明专利

JPS5741903B2 失效
公开(公告)号：JPS5741903B2
公开(公告)日：1982-09-06
申请号：JP5605479
申请日：1979-05-08
IPC分类号： C12N1/16 , C12N1/20 , C12N1/38 , C12P7/66 , C12R1/01 , C12R1/05 , C12R1/38 , C12R1/645 , C12R1/72 , C12R1/78 , C12R1/84 , C12R1/88

52. 发明专利

JPS5738240B2 失效
公开(公告)号：JPS5738240B2
公开(公告)日：1982-08-14
申请号：JP10799480
申请日：1980-08-06
IPC分类号： C12P7/66 , C12R1/01

53. 发明专利

JPS5726754B2 失效
公开(公告)号：JPS5726754B2
公开(公告)日：1982-06-07
申请号：JP6573980
申请日：1980-05-16
IPC分类号： C12P7/66 , C12R1/645
摘要： Difficulties with known techniques of producing coenzyme Q10, for example low yields of the coenzyme, are overcome by cultivating a microorganism of the genus Aureobasidium or Trichosporon in a culture medium containing a large quantity of p-hydroxybenzoic acid. Ethanol can be added to the culture medium as the main carbon source. The coenzyme that is produced can be used in the treatment of heart diseases, hypertension and tumors.

54. 发明专利

JPS5779891A Preparation of coenzyme q10 失效
标题翻译： COYZYME Q10的制备
公开(公告)号：JPS5779891A
公开(公告)日：1982-05-19
申请号：JP9629781
申请日：1981-06-22
申请人： Mitsubishi Gas Chem Co Inc
发明人： URAGAMI SADAJI
IPC分类号： C12P7/66 , C12R1/01
摘要： PURPOSE: To prepare the titled substance useful as a pharmaceutical or feed additive, by culturing bacteria belonging to Protaminobacter methanolica and capable of producing the titled substance, and separating the objective substance from the bacterial cells.
CONSTITUTION: Novel bacteria capable of producing coenzyme Q
10 e.g. Protaminobacter methanolica BP-3 (FERM-P No.4893), Protaminobacter methanolica BP-11 (FERM-P No.4894), etc. are cultured in a medium containing carbon sources such as glucose, xylose, methanol, etc., nitrogen sources such as urea, nitrates, etc., and other inorganic components, and the cultured bacterial cells are separated from the medium. Coenzyme Q
10 is extracted from the bacterial cell by conventional extraction technique such as ethanol extraction, etc., and fractionated and purified to obtain the objective substance.
COPYRIGHT: (C)1982,JPO&Japio
摘要翻译：目的：制备可用作药物或饲料添加剂的标题物质，通过培养属于甲醇氨基葡萄糖胺的细菌并能够制备标题物质，并将目标物质与细菌细胞分离。构成：能够产生辅酶Q10的新型细菌，例如甲醇葡聚糖BP-3（FERM-P No.4893），甲醇氨基葡聚糖BP-11（FERM-P No.4894）等在含有葡萄糖，木糖，甲醇等碳源，氮来源如尿素，硝酸盐等，以及其它无机组分，培养的细菌细胞与培养基分离。辅酶Q10通过常规提取技术如乙醇提取等从细菌细胞中提取，并分级纯化得到目标物质。

55. 发明专利

JPS5721308B2 JPS5721308B2 - 失效
公开(公告)号：JPS5721308B2
公开(公告)日：1982-05-06
申请号：JP3365980
申请日：1980-03-17
IPC分类号： C12P7/66

56. 发明专利

JPS5763081A Tissue culture of boarginaceae 失效
标题翻译：组织的组织文化
公开(公告)号：JPS5763081A
公开(公告)日：1982-04-16
申请号：JP13676980
申请日：1980-10-02
申请人： Mitsui Petrochem Ind Ltd
发明人： TABATA MAMORU , YOSHIKAWA NOBUJI
IPC分类号： C12N5/04 , C12N5/02 , C12P7/66 , C12R1/91
摘要： PURPOSE: Using a liquid culture medium containing algae, an efficient tissue culture is conducted on a Boraginaceae plant containing naphthoquinone piments such as cinchonine.
CONSTITUTION: A plant in Boraginaceae such as Lithospermum erythrorhizon Siev. et Zucc is subjected to tissue culture in a liquid culture medium containing about 0.1W100g, preferably about 2W50g of agar per liter of the liquid medium.
COPYRIGHT: (C)1982,JPO&Japio
摘要翻译：目的：使用含有藻类的液体培养基，对含有萘醌类药物（例如辛可宁）的紫草科植物进行有效的组织培养。构成：紫草科植物，如紫草科植物。将Zucc在含有约0.1-100g，优选约2-50g琼脂每升液体培养基的液体培养基中进行组织培养。

57. 发明专利

JPS5739779A Callus culture of plant belonging to boraginaceae family 失效
标题翻译：植物的铜绿色文化与BORAGINACEAE家族相关
公开(公告)号：JPS5739779A
公开(公告)日：1982-03-05
申请号：JP11590380
申请日：1980-08-25
申请人： Mitsui Petrochem Ind Ltd
发明人： OGINO TAKAO , HARA YASUHIRO
IPC分类号： C12N5/04 , A61K36/18 , C12N5/02 , C12P7/66 , C12R1/91
摘要： PURPOSE: To prepare naphthoquinone pigment and other useful materials, in large quantities, efficiently, by the callus culture of a plant belonging to Boraginaceae family in a liquid medium having controlled ratio of ammonium ion in the nitrogen source.
CONSTITUTION: The callus culture of a plant belonging to Boraginaceae family, e.g. Lithospermum erythrorhizon Sieb. et Zucc, is carried out in a liquid medium such as Linsmeier-Skoog medium, etc., which contains a nitrogen source containing ≤10mol%, pref. ≤ about 5mol% of ammonium ions, or esp. a nitrogen source essentially free from ammonium ion.
COPYRIGHT: (C)1982,JPO&Japio
摘要翻译：目的：通过在氮源中具有铵离子控制比例的液体培养基中，通过在属于白蛉科的植物的愈伤组织培养，大量有效地制备萘醌颜料和其它有用的材料。构成：属于紫草科的植物的愈伤组织培养物，例如紫草红 et Zucc在液体培养基如Linsmeier-Skoog培养基等中进行，其含有含有<= 10mol％的氮源。 <=约5mol％的铵离子，或特别是基本上不含铵离子的氮源。

58. 发明专利

JPS574938A NOVEL ANTHRACYCLINE ANTIBIOTIC SUBSTANCE 失效
公开(公告)号：JPS574938A
公开(公告)日：1982-01-11
申请号：JP7934680
申请日：1980-06-12
申请人： SANRAKU OCEAN CO
发明人： OKI SHIYUNICHI , YOSHIMOTO AKIHIRO , MATSUZAWA YASUE , KOUNO KAGEAKI , INUI TAIJI , TAKEUCHI TOMIO , UMEZAWA HAMAO
IPC分类号： C12P17/06 , C07C45/00 , C07C50/22 , C07C67/00 , C07D309/14 , C07H15/252 , C12P7/66 , C12R1/465
摘要： NEW MATERIAL:An anthracycline antibiotic substance, feudomycin B, of formula I (R is CH2COCH3 or CH3; R is group of formula II or H; when R is CH3, R is H), and feudomycin aglycone B and C. USE:Carcinostatic agent which remarkably suppresses the proliferation of mouse leukemia cell L1210 and the synthesis of uncleic acid. It is also an intermediate which can be converted to active alycoside compound by microbial conversion or chemical conversion. PROCESS:A mutant No. 4N-140 (FERM-P No.5520) originated from Streptomyces coeruleorubidus ME130-A4 (FERM-P No.3540) is cultured serobically in a nutrient medium for actinomycetes containing a carbon source, a nitrogen source, inorganic cimponent, and trace component, at 20-40 C. The compound of formula III (R( is CH2CH3 or CH2COCH3) separated from the cultured product by convertional process is hydrolyzed with an acid to obtain the compound of formula IV (R is R or R ).

59. 发明专利

JPS571232B2 失效
公开(公告)号：JPS571232B2
公开(公告)日：1982-01-09
申请号：JP2176078
申请日：1978-02-27
IPC分类号： C12P7/66 , C12R1/01

60. 发明专利

JPS56154994A PREPARATION OF COENZYME Q10 失效
公开(公告)号：JPS56154994A
公开(公告)日：1981-11-30
申请号：JP5842080
申请日：1980-04-30
申请人： SEKISUI CHEMICAL CO LTD
发明人： SHIROZA SATOSHI , SAKATA NOBUTSUGU , MORIKANE YASUMASA , OOTA JIYUNZOU
IPC分类号： C12P7/66 , C12R1/645 , C12R1/72
摘要： PURPOSE:A yeast containing the titled substance is cultured in the presence of linalool to facilitate the isolation and purification of the titled substance, thus producing the objective coenzyme in high yield. CONSTITUTION:A yeast capable of producing coenzyme Q10, which belongs to Candida, Torulopsis, Rhodotorula or Cryptococcus, is aerobically culutured in a general-purpose medium containing carbon sources as glucose and nitrogen sources as ammonium sulfate. Linalool is dissolved in 1ml of ethanol by an amount necessary for 1l of the culture medium and added to the medium, resultingly about 1.5- 50mg/g of the produced dry cells is appropriate. After the cultivation, the cells are separated, extracted with hexane, then the coenzyme is separated and purified by chromatography.

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