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51. 发明专利

JPS61282321A INFLUENZA VACCINE 失效
公开(公告)号：JPS61282321A
公开(公告)日：1986-12-12
申请号：JP12334185
申请日：1985-06-06
申请人： NAT INST HEALTH , CHEMO SERO THERAPEUT RES INST , DAIICHI SEIYAKU CO
发明人： NEROME KUNIAKI , OTANI AKIRA , OKUMA KUNIO , INOUE ATSURO
IPC分类号： A61K39/102 , A61K9/127 , A61K9/50 , A61K39/145 , A61K39/39 , A61P31/12 , A61P31/16
摘要： NEW MATERIAL:The complex of influenza HANA antigen and a derivative of muramyldipeptide (abbreviated as MDP). EXAMPLE:HANA-B30-MDP complex. USE:An influenza vaccine. It has improved immunogenicity compared with known vaccine as an artificial membrane vaccine like the influenza virus particle, i.e. a virosome vaccine. PREPARATION:Influenza HANA antigen is mixed with an MDP derivative such as the compound of formula (Q is 20-60C synthetic higher fatty acid residue; A is L-alanine, L-serine, etc.; isoGLn is isoglutamine) at a weight ratio of 10/1-1/300 in a phosphate buffer solution. The mixed liquid is solubilized with 0.1-10W/V% surfactant. The surfactant is removed from the solubilized product by dialysis to obtain the objective complex of HANA antigen and MDP derivative.

52. 发明专利

JPS61260024A PREVENTIVE FOR AUJESZKY'S DISEASE 失效
公开(公告)号：JPS61260024A
公开(公告)日：1986-11-18
申请号：JP10210485
申请日：1985-05-13
申请人： CHEMO SERO THERAPEUT RES INST
发明人： NISHIKAWA HIROSHI , MATSUO KAZUO , SAKAMOTO SHINICHI , YAMADA SHINJI
IPC分类号： C07K14/03 , A61K39/245 , A61K39/395 , A61P31/12 , C07K14/435
摘要： PURPOSE:To provide the titled preventive composed of a glycoprotein component originated from the antigen of Aujeszky's disease virus and separated by using a specific monoclonal antibody. CONSTITUTION:A glycoprotein (GP) component originated from the antigen of Aujeszky's disease is separated from the antigen of Aujeszky's disease by using a monoclonal antibody (MCA-AU1) recognizing the GP exhibiting the band corresponding to the molecular weight of 105-125K and a band of 65-75K by Western immunoblot method or a monoclonal antibody (MCA-AU2) recognizing the GP exhibiting the band corresponding to the molecular weight of 85-95K as an immunoadsorbent, and the separated GP component is used as an active component of the objective remedy for Aujeszky's disease. The MCA-AU1 and AU2 have high neutralization activity by themselves and are useful for the prevention and remedy of the above disease. GP can be administered parenterally and a large amount of antigen can be administered without fear of side effects.

53. 发明专利

JPS61220750A CENTRIFUGAL SEPARATOR 失效
公开(公告)号：JPS61220750A
公开(公告)日：1986-10-01
申请号：JP5573185
申请日：1985-03-22
申请人： PENNWALT CORP , CHEMO SERO THERAPEUT RES INST
发明人： FUKUSHIRO SHINICHI , KUBO AKITSUGU , HASEGAWA KAZUMITSU , MURAOKA TOYOJI , INOUE HIROAKI
IPC分类号： B04B9/12 , B04B1/02

54. 发明专利

JPS61195346A ELECTRODE FILM FOR IMMUNE SENSOR 失效
公开(公告)号：JPS61195346A
公开(公告)日：1986-08-29
申请号：JP3683985
申请日：1985-02-25
申请人： CHEMO SERO THERAPEUT RES INST
发明人： TANIGUCHI ISAO , YASUKOCHI KAZUO , TSUJI ICHIRO
IPC分类号： G01N27/40 , A61K39/00 , G01N27/30 , G01N27/327 , G01N33/543 , G01N33/545
摘要： PURPOSE:To obtain an electrode film for an immune sensor which exhibits a high membrane potential change and good responsiveness with high sensitivity by forming a polymerized film of pyrrole or thiophene and fixing an antigen or antibody to the surface of such film. CONSTITUTION:Pyrrole or thiophene is dissolved in a solvent such as acetonitrile which dissolves the same but insoluble in the polymers thereof and tetraalkyl ammonium perchlorate or the like is added as an electrolyte to such soln. to prepare an electrolytic polymn. soln. A working electrode and counter electrode are immersed into the soln. prepd. in such a manner and the soln. is electrolytically polymerized, by which the polypyrrole or polythiophene film is formed on the working electrode. The film is brought into contact with an antigen- or antibody-contg. liquid as it is or after the film is stripped from the electrode so that the antigen or antibody is conjugated and immobilized to the film. The measurement of the antibody or antigen concn. with good responsiveness is made possible by, for example, immersing the working electrode 10 obtd. in such a manner into the soln. 12 contg. the antibody or antigen and measuring the potential change by the antigen-antibody reaction while stirring the soln. by a magnetic stirrer 15.

55. 发明专利

JPS61162944A EXPERIMENTAL ANIMAL FIXING TOOL 失效
公开(公告)号：JPS61162944A
公开(公告)日：1986-07-23
申请号：JP371085
申请日：1985-01-11
申请人： CHEMO SERO THERAPEUT RES INST
发明人： TAKESHITA MINORU
IPC分类号： A61D3/00 , A01K1/03

56. 发明专利

JPS6165162A Non-specific reactive absorvent in immunilogical measuring method using monoclonal antibody 失效
标题翻译：使用单克隆抗体的免疫测定方法中的非特异性反应性吸收
公开(公告)号：JPS6165162A
公开(公告)日：1986-04-03
申请号：JP18717084
申请日：1984-09-05
申请人： Chemo Sero Therapeut Res Inst
发明人： TSUJI ICHIRO , UNOKI MASANORI , AOYAMA NORIAKI
IPC分类号： G01N33/53 , G01N33/577
CPC分类号： G01N33/577 , G01N33/5306
摘要： PURPOSE: To obtain exact measurement data with a substantial absorption effect by using the mouse serum, mouse ascites, rat serum or rat ascites as a non-specific reactive absorbent.
CONSTITUTION: The mouse serum, mouse ascites, rat serum or rat ascites is used as the non-specific reactive absorbent in an immunological measuring method using the mouse-derived monoclonal antibody (MCA) obtd. by cloning the hydridoma obtd. by confluence of the mouse spleen cells immunized by various antigens and mouse myeloma. Such absorbent exhibits a satisfactory effect even at 0.1V/V% addition amt. and is more preferably used at 0.5W1.0V/V%. Then the immune globulin contained in the mouse serum, mouse ascites, rat serum or rat ascites eracts with the anti-mouse immune globulin in the specimen and absorbs. the same. This absorbent absorbs thoroughly said globulin regardless of the intensity of the non-specific reaction and does not affect the measurement of the specimen which does not exhibit the non- specific reaction.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过使用小鼠血清，小鼠腹水，大鼠血清或大鼠腹水作为非特异性反应性吸收剂，获得具有显着吸收作用的精确测量数据。构成：使用小鼠来源的单克隆抗体（MCA），在免疫学测定方法中使用小鼠血清，小鼠腹水，大鼠血清或大鼠腹水作为非特异性反应性吸收剂。通过克隆hydridoma obtd。通过由各种抗原和小鼠骨髓瘤免疫的小鼠脾细胞的汇合。即使在0.1V / V％的添加量下，这种吸收剂仍然表现出令人满意的效果。并且更优选以0.5-1.0V / V％使用。然后，小鼠血清，小鼠腹水，大鼠血清或大鼠腹水中含有的免疫球蛋白与样品中的抗小鼠免疫球蛋白一起被吸收。一样。该吸收剂无论非特异性反应的强度如何，都能充分吸收所述球蛋白，并且不影响不显示非特异性反应的样品的测量。

57. 发明专利

JPS6154450A Gel for affinity chromatography having group specificity and its production 失效
标题翻译：具有组织特性及其生产的亲和色谱的凝胶
公开(公告)号：JPS6154450A
公开(公告)日：1986-03-18
申请号：JP17734784
申请日：1984-08-24
申请人： Chemo Sero Therapeut Res Inst
发明人： KAWAHARA TETSUO , MIZUNO KYOSUKE , SHIN SADAO , MIZOGAMI HIROSHI
IPC分类号： B01J20/281 , B01J20/291 , C08B5/14 , C08B37/00 , C12P21/00 , G01N30/88
CPC分类号： B01J20/291 , B01J2220/54
摘要： PURPOSE:To obtain a gel for chromatography which maintains a solid granular state, is insoluble in an aq. solvent and has excellent physical stability by esterifying non-crosslinked cellulose by sulfuric acid. CONSTITUTION:A esterifying agent such as sulfuric acid is dissolved in amine or amide and the granular material of the non-crosslinked cellulose is brought into reaction therewith and is then neutralized with an alkali. Sulfuric anhydride or chlorosulfonic acid is preferable as the esterifying agent such as sulfuric acid and is used at about 1-50pts. (pts.wt., hereafter the same) in 100pts. the raw material cellulose and the reaction is effected at about -10-100 deg.C for about 30min-6hr. The amine or amide to be used as the solvent is exemplified by pyridine, dimethylformamide, etc. and the raw material cellulose to be added thereto so as to react therewith is used after the commercially marketed granular material having about 15-150mum grain size for chromatography is dried to about 1% moisture content. The resultant gel for affinity chromatography consists of the water-insoluble solid granular particles of the non-crosslinked cellulose sulfate having 15-150mum grain size.
摘要翻译：目的：为了获得保持固体颗粒状态的色谱凝胶，不溶于水溶液。溶剂，并通过硫酸使非交联纤维素酯化而具有优异的物理稳定性。构成：将硫酸等酯化剂溶解在胺或酰胺中，使非交联纤维素的粒状物质与其反应，然后用碱中和。硫酸酐或氯磺酸作为酯化剂如硫酸是优选的，并且在约1-50pts下使用。（pts.wt.，以下相同）在100pts。原料纤维素和反应在约-10-100℃进行约30分钟-6小时。用作溶剂的胺或酰胺的例子有吡啶，二甲基甲酰胺等，加入其中与其反应的原料纤维素在具有约15-150μm粒度的商业上销售的颗粒状物质用于色谱法干燥至约1％水分含量。用于亲和层析的所得凝胶由具有15-150μm粒度的非交联的硫酸纤维素的水不溶性固体颗粒颗粒组成。

58. 发明专利

JPS6147185A Method of purifying japanese b encephalitis virus 失效
标题翻译：净化日本乙肝病毒的方法
公开(公告)号：JPS6147185A
公开(公告)日：1986-03-07
申请号：JP16732384
申请日：1984-08-09
申请人： Chemo Sero Therapeut Res Inst
发明人： SAKAMOTO KUNIAKI , GOTO ISAO , KAWAHARA TETSUO , SAKO MITSUO
IPC分类号： C12N15/09 , A61K39/12 , C12N7/00 , C12N7/02 , C12R1/91
CPC分类号： C12N7/00 , A61K39/12 , A61K2039/5252 , C12N2770/24134 , C12N2770/24151
摘要： PURPOSE: To purify Japanese B encephalitis virus in high yield in high purity, by bringing a Japanese B encephalitis virus-containing solution into contact with cellulose sulfate gel, adsorbing the Japanese B encephalitis gel on the gel, eluting it from the gel.
CONSTITUTION: In purity Japanese B encephalitis virus, a Japanese B encephalitis virus-containing solution (including virus obtained from brain material of animal infected with Japanese B encephalitis or culture material of infectious tissue culture cell, or Japanese B encephalitis virus material obtained by using gene recombinant) is brought into contact with cellulose sulfate gel, the Japanese B encephalitis virus is adsorbed on it, the Japanese B encephalitis virus is eluted from the cellulose sulfate gel, to purify the Japanese B encephalitis virus. Cellulose sulfate obtained from crystalline cellulose consisting of crystal region and amorphous region is preferable as the cellulose sulfate.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过将日本B型脑炎病毒溶液与硫酸纤维素凝胶接触，以高纯度纯化日本B型脑炎病毒，将日本B型脑炎凝胶吸附在凝胶上，从凝胶中洗脱。构成：在纯度日本B型脑炎病毒中，含有日本B型脑炎病毒的溶液（包括从日本B型脑炎感染的动物的脑材料获得的病毒或感染性组织培养细胞的培养物质，或通过使用基因得到的日本B型脑炎病毒物质重组体）与硫酸纤维素凝胶接触，日本B型脑炎病毒被吸附在其上，日本B型脑炎病毒从硫酸纤维素凝胶上洗脱，以纯化日本B型脑炎病毒。作为硫酸纤维素，优选由结晶区域和无定形区域构成的结晶纤维素得到的硫酸纤维素。

59. 发明专利

JPS6128391A Recombinant plasmid integrated with simple herpes virus, transformed yeast, and preparation of simple herpes virus protein 失效
标题翻译：与简单的病毒，转化的YEAST和简单的病毒性病毒蛋白质的制备重组的PLASMID
公开(公告)号：JPS6128391A
公开(公告)日：1986-02-08
申请号：JP15176684
申请日：1984-07-20
申请人： Chemo Sero Therapeut Res Inst
发明人： NOZAKI CHIKAHIDE , KINO YOICHIRO , ETO TATSUO , MAKIKADO KEIICHI , OTOMO SHINYA
IPC分类号： C12N15/09 , A61K39/00 , A61K39/245 , C07H21/04 , C07K14/035 , C12N1/16 , C12N15/81 , C12P21/00 , C12R1/865
CPC分类号： C12N15/81 , A61K39/00 , A61K39/12 , A61K39/245 , C07K14/005 , C12N2710/16622 , C12N2710/16634
摘要： PURPOSE:To mass produce simple herpes virus protein useful for producing a vaccine for preventing simple herpes virus infectious diseases, by using genetic engineering technology. CONSTITUTION:A shuttle vector having both Eschericha coli and a yeat genes, capable of multiplying in either of them, is used, part or the whole of acidic phosphatase structure gene or fixed sites in the upper stream of it is removed in the downstream of the acidic phosphatase supported by the vector, and a recombinant plasmid integrated with simple herpes virus gB gene is obtained. Simple herpes virus gene developed plasmid thus prepared is treated with a yeast by a conventional produced, and transformed, to give a transformant yeast. The transformant yeast is cultivated preferably under a condition where an acidic phosphatase promoter is not controlled, to give the aimed protein.
摘要翻译：目的：通过使用基因工程技术，大量生产用于生产用于预防简单疱疹病毒感染性疾病的疫苗的简单疱疹病毒蛋白。构成：使用具有两种大肠杆菌和酵母基因的穿梭载体，其能够在其中任一种中繁殖，部分或全部酸性磷酸酶结构基因或其上游中的固定位点在其下游被去除得到载体支持的酸性磷酸酶，与简单疱疹病毒gB基因整合的重组质粒。将由此制备的简单疱疹病毒基因开发的质粒通过常规产生的酵母进行处理并转化，得到转化酵母。转化酵母优选在不控制酸性磷酸酶启动子的条件下培养，得到目标蛋白质。

60. 发明专利

JPS60263859A INDIRECT REAGENT FOR INSPECTING HEMAGGLUTINATION 失效
公开(公告)号：JPS60263859A
公开(公告)日：1985-12-27
申请号：JP12044084
申请日：1984-06-11
申请人： CHEMO SERO THERAPEUT RES INST
发明人： AOYAMA NORIAKI , SAITOU SEIICHI
IPC分类号： G01N33/543 , G01N33/555 , G01N33/86
摘要： PURPOSE:To obtain the heterophil antibody absorbing effect equal to the effect of the stroma membrane piece of a goat, etc. by allowing the pulverous particles of the organ, more particularly kidney, etc. of guinea pig to oxist in a diluent. CONSTITUTION:The fat component, etc. surrounding the organ, particularly, kidney, etc. of the guinea pig taken out of the body are first removed therefrom. The organ is thereafter cut to a suitable size and is cleaned in a physiological salt soln., etc. by which the blood is removed therefrom. The aq. suspension of the fine pieces of the guinea pig organ is heated for about 1hr in a water bath, preferably under a boiling condition in the stage of subjecting said suspension to a heating treatment. On the other hand, a hydrophilic solvent such as alcohol is used in the stage of a solvent treatment and the fine pieces of the organ are dipped in the solvent under stirring or are rested therein by which the fine pieces thereof are treated for about 30min-2hr. The fine pieces treated in such a manner are cleaned with the physiological salt soln., etc. by which the fats and other impurities are removed and the purer effective component is obtd. The cleaned fine pieces are further suspended in about 3-fold the volume of PBS liquid, etc. and are processed by suitable disintegrating means to obtain the suspension contg. about

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