会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 34. 发明专利
    • COUNTING OF LIVE CELL OF MICROORGANISM
    • JPH03272697A
    • 1991-12-04
    • JP29905690
    • 1990-11-06
    • MITSUBISHI HEAVY IND LTD
    • SUGATA KIYOSHIUEDA RYOHEIDOI TAKASHIONISHI TAKASHIMATSUMOTO KAZUNORI
    • C12M1/34C12Q1/06G01N21/64
    • PURPOSE:To count the number of the title live cells by minute unit by irradiating a cell, through which cell specimens are passed, with excitation light from a light source having wavelength to excite a fluorescent substance accumulated in the cells, receiving fluorescence emitted from the cells by a receptor element and detecting the output by a counter. CONSTITUTION:A target measurement specimen containing cells of microorganism is sent from a line P1 to a reactor V1, a solution of a fluorescent substance such as fluorescen diacetate (FDA) in acetone is fed from a line P2 to the reactor V1, live cells in the specimen are reacted with FDA in the reactor V1 to accumulate fluorescein in the cells, the fluorescein is fed to a rectangular cell V2 having a crosssectional shape which is perpendicular to flow direction of the measurement part and long in the lateral direction and short in the longitudinal direction, the cell V2 is irradiated with excitation light from a light source 1 having wavelength required to excite the fluorescent substance accumulated in the cells, fluorescence emitted from the cells from the front part long in the lateral direction is received by a receptor element 7 and the output of the receptor element 7 is measured by a pulse counter 9 to count the number of microorganism cells.
    • 35. 发明专利
    • METHOD FOR COUNTING LIVING MICROBIAL CELLS
    • JPH03244395A
    • 1991-10-31
    • JP3837190
    • 1990-02-21
    • MITSUBISHI HEAVY IND LTD
    • SUGATA KIYOSHIUEDA RYOHEI
    • G01N21/64C12Q1/06G01N21/77
    • PURPOSE:To count the number of living microbial cells in high sensitivity by adding a fluorescein derivative dissolved in a solvent to a specimen, keeping the mixture at a prescribed temperature for a prescribed time, lowering the pH of the mixture with an acid and irradiating the specimen with an excitation light. CONSTITUTION:A fluorescein derivative dissolved in a solvent is added to a specimen and maintained at a prescribed temperature for a prescribed time. The pH of the mixture is lowered with an acid, the specimen is irradiated with an excitation light and the number of the living microbial cells is counted by detecting the fluorescence emitted from the cell. As an alternative method, a fluorescein derivative dissolved in a solvent and free from dilution with a phosphoric acid buffer solution is added to a specimen and maintained at a prescribed temperature for a prescribed time. The pH of the mixture is lowered with an acid, the specimen is irradiated with an excitation light and the number of the living microbial cells is counted by detecting the light emitting from the cell.
    • 37. 发明专利
    • TREATMENT OF WATER HAVING COLOR OF RAW SEWAGE
    • JPH01293193A
    • 1989-11-27
    • JP11923188
    • 1988-05-18
    • MITSUBISHI HEAVY IND LTD
    • SUGATA KIYOSHIOGAWA NAOKI
    • C02F3/12C02F3/34
    • PURPOSE:To decolor water having the color of raw sewage by carrying out integrated culture with water obtd. by treating raw sewage as a tracing source and a culture ground contg. water having the color of raw sewage, adding agar to the ground, carrying out screening culture, inoculating the resulting colony into a similar culture ground, carrying out shaking culture and treating the water having the color of raw sewage with the resulting two or more kinds of strains which are very effective in color separation of the color of raw sewage. CONSTITUTION:Integrated culture is carried out with soil, activated sludge or water obtd. by treating raw sewage as a tracing source and a culture ground consisting of water having the color of raw sewage, an extract of yeast, MgSO4.7H2O, KH2PO4 and NH4Cl. Agar is then added to the ground and screening culture is carried out. A colony formed on the agar is inoculated into a similar culture ground and shaking culture is carried out. Yellowish brown water obtd. by treating raw sewage is treated with basidiomycete and bacterium strains having >=15% rate of color separation of the color of raw sewage selected among the resulting strains. The yellowish brown water can be decolored at a low cost by the action of microorganisms in place of the conventional physicochemical method using ozone and activated carbon.