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31. 发明专利

JP2006020514A Method for identifying base polymorphism 审中-公开
标题翻译：识别基因多态性的方法
公开(公告)号：JP2006020514A
公开(公告)日：2006-01-26
申请号：JP2004198911
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting base polymorphism clearly and simply, capable of obtaining a result rapidly and easily in a good reproducibility by suppressing a non-specific bonding reaction as low.
SOLUTION: This method for detecting the base polymorphism contained in a specimen solution by performing an amplifying reaction by using a specific nucleic acid sequence-amplifying oligonucleotide in a specimen solution containing a specific nucleic acid sequence and at the same time with or after the amplification reaction, bringing the above in contact with a labeled oligonucleotide complementary bonding with the base polymorphic part of the nucleic acid sequence and detecting the labeled oligonucleotide bonded with the specific nucleic acid sequence, or detecting the amplified nucleic acid sequences through the labeled oligonucleotide is characterized by presenting an oligonucleotide having a sequence with at least one position different from the labeled oligonucleotide complementary bonding with the base polymorphic part, and having a higher Tm value than that of the labeled oligonucleotide complementary bonding with the base polymorphic part.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供一种清楚简单地检测碱基多态性的方法，能够通过将非特异性结合反应抑制得低，从而以良好的再现性快速且容易地获得结果。解决方案：通过在含有特定核酸序列的试样溶液中进行扩增反应，同时使用特异性核酸序列扩增寡核苷酸，同时进行扩增反应，检测试样溶液中含有的碱基多态性的方法扩增反应，使上述与核酸序列的碱基多态性部分互补结合的标记寡核苷酸接触，检测与特异性核酸序列键合的标记寡核苷酸，或者通过标记的寡核苷酸检测扩增的核酸序列，其特征在于，具有与所述碱基多态性部分互补结合的标记寡核苷酸至少一个位置的序列的寡核苷酸，并且具有比与所述碱基多态性部分互补结合的标记寡核苷酸具有更高的Tm值。版权所有（C）2006，JPO＆NCIPI

32. 发明专利

JP2006020513A Method for detecting nucleic acid 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：JP2006020513A
公开(公告)日：2006-01-26
申请号：JP2004198910
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/50
摘要： PROBLEM TO BE SOLVED: To provide a method for clearly and simply detecting a nucleic acid sequence rapidly and precisely in a good reproducibility without requiring complex operations.
SOLUTION: This method for detecting the nucleic acid sequence by using a liquid-passing filter, by performing an amplification reaction by using an oligonucleotide for a specific nucleic acid sequence amplification in a specimen solution containing the specific nucleic acid sequence, at the same time with or after the amplification reaction, bringing the above in contact with a bonded material-bonded with a second ligand capable of specifically bonding with the nucleic acid sequence and detecting the complex bonded with the bonded material comprises a process of bonding the first ligand part of the complex with a liquid-passing filter bonded with a first ligand-capturing agent, a process of bonding a labeled physiologically active substance with the second ligand bonded with the liquid-passing filter through the first ligand-capturing agent and the ligand part and a process of measuring the label bonded with the liquid-passing filter.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种清楚简单地以良好重现性快速准确地检测核酸序列而不需要复杂操作的方法。解决方案：通过使用通过滤器来检测核酸序列的方法，通过在含有特定核酸序列的试样溶液中使用特异性核酸序列扩增的寡核苷酸进行扩增反应在与扩增反应之间或之后相同的时间，使上述与与能够与核酸序列特异性结合的第二配体键合的键合材料接触并检测与键合材料键合的复合物包括将第一配体具有与第一配体捕获剂结合的液体通过过滤器的复合物的一部分，将标记的生理活性物质与通过第一配体捕获剂和配体部分与液体通过过滤器结合的第二配体键合的方法以及测量与液体通过过滤器结合的标签的过程。版权所有（C）2006，JPO＆NCIPI

33. 发明专利

JP2006020511A Method for detecting nucleic acid 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：JP2006020511A
公开(公告)日：2006-01-26
申请号：JP2004198908
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12M1/00 , C12N15/09 , G01N33/50
摘要： PROBLEM TO BE SOLVED: To provide a method for clearly and simply detecting a nucleic acid sequence in a specimen, capable of obtaining rapidly and easily a result in a good reproducibility, since without requiring complex operations.
SOLUTION: This method for detecting the nucleic acid sequence contained in the specimen solution by performing an amplification reaction by using an oligonucleotide for the nucleic acid sequence amplification in a specimen solution containing the specific nucleic acid sequence, at the same time with or after the amplification reaction, bringing the above in contact with an oligonucleotide bonded with a ligand capable of specifically bonding with the nucleic acid sequence and detecting the ligand bonded with the specific nucleic acid sequence is characterized by using a liquid-passing filter bonded with the oligonucleotide specifically recognizing the amplified nucleic acid sequence, and a labeled physiologically active substance.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种清楚简单地检测样本中的核酸序列的方法，其能够快速且容易地获得良好的再现性，因为不需要复杂的操作。解决方案：该方法通过在含有特定核酸序列的样品溶液中使用寡核苷酸进行扩增反应来检测样品溶液中所含的核酸序列，同时具有或扩增反应后，使上述与与能够与核酸序列特异性结合的配体键合的寡核苷酸接触并检测与特异性核酸序列键合的配体的特征在于使用与寡核苷酸结合的液体通过过滤器特异性识别扩增的核酸序列和标记的生理活性物质。版权所有（C）2006，JPO＆NCIPI

34. 发明专利

JP2006020510A Method for identifying base polymorphism 审中-公开
标题翻译：识别基因多态性的方法
公开(公告)号：JP2006020510A
公开(公告)日：2006-01-26
申请号：JP2004198907
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting base polymorphism clearly and simply and obtaining a result in a good reproducibility quickly and easily.
SOLUTION: This method for detecting the base polymorphism by performing an amplifying reaction by using an amplifying oligonucleotide bonded with a first ligand in a specimen solution containing a specific nucleic acid sequence and bringing a bonded material bonded with a second ligand in contact with the above after the amplification reaction and detecting a complex bonded with a second ligand-bonded material bonded with the specific nucleic acid sequence comprises a process of bonding the first ligand part of the complex with a first ligand-capturing agent by dripping the complex on the surface of a liquid-passing filter bonded with the first ligand-capturing agent, a process of dripping a solution of a labeled physiologically active substance capable of bonding with the second ligand for bonding the labeled physiologically active substance with the second ligand bonded with the liquid-passing filter through the first ligand-capturing agent and the ligand part and a process for titrating the label bonded with the liquid-passing filter.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种清楚简单地检测碱基多态性并快速且容易地获得良好重现性的结果的方法。解决方案：通过使用与含有特定核酸序列的试样溶液中的与第一配体结合的扩增寡核苷酸进行扩增反应并使与第二配体键合的键合材料与第二配体接触的方法来检测碱基多态性的方法上述扩增反应后并检测与与特定核酸序列键合的第二配体键合材料结合的复合物包括通过滴加复合物将复合物的第一配体部分与第一配体捕获剂结合的方法与第一配体捕获剂结合的液体通过过滤器的表面，滴加与第二配体结合的标记的生理活性物质的溶液以将标记的生理活性物质与用液体结合的第二配体结合的方法通过第一配体捕获剂和配体部分的过滤器以及用于ti的方法挑选与液体通过过滤器粘合的标签。版权所有（C）2006，JPO＆NCIPI

35. 发明专利

JP2005027517A Simple method for detecting human cytochrome p4502a6 genetic polymorphism and reagent for detection 审中-公开
标题翻译：用于检测人细胞色素P4502A6遗传多态性的简单方法和用于检测的试剂
公开(公告)号：JP2005027517A
公开(公告)日：2005-02-03
申请号：JP2003193556
申请日：2003-07-08
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method with which drug metabolic enzyme genetic polymorphism can definitely be detected with good reproducibility by simple operation and to provide a reagent therefor.
SOLUTION: The method for detecting the genetic polymorphism in exon 3 of a human cytochrome P4502A6 gene is characterized as using an oligonucleotide containing a sequence substantially complementary to any one of specific base sequences. The genetic polymorphism in the exon 3 of the human cytochrome P4502A6 gene is preferably detected by amplifying a sample containing the gene and/or its fragment.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种通过简单操作可以以良好的再现性确定地检测药物代谢酶遗传多态性并提供其试剂的方法。解决方案：用于检测人细胞色素P4502A6基因的外显子3中的遗传多态性的方法的特征在于使用含有与任何一个特定碱基序列基本上互补的序列的寡核苷酸。优选通过扩增含有该基因和/或其片段的样品来检测人细胞色素P4502A6基因的外显子3中的遗传多态性。版权所有（C）2005，JPO＆NCIPI

36. 发明专利

JP2004236655A Method for simply detecting genetic polymorph and reagent for detecting the same 审中-公开
公开(公告)号：JP2004236655A
公开(公告)日：2004-08-26
申请号：JP2003203722
申请日：2003-07-30
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To product a method for clearly detecting genetic polymorphs in several sites, especially the genetic polymorphs of cytochrome 2D6, 2C19, etc., in several sites, with good repeatability, and to provide a reagent for the same. SOLUTION: This method for detecting the genetic polymorphs comprises conducting amplification by using any one of a wild type-detecting oligonucleotide and a variant-type detecting oligonucleotide and a nucleic acid-specific label, wherein a sequence having at least two variation sites different from each other is detected under an identical amplification condition. COPYRIGHT: (C)2004,JPO&NCIPI

37. 发明专利

JP2004201676A Method for simply detecting gene polymorphism and reagent for detecting the same 审中-公开
标题翻译：用于简单检测基因多态性和检测其的试剂的方法
公开(公告)号：JP2004201676A
公开(公告)日：2004-07-22
申请号：JP2003315800
申请日：2003-09-08
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting gene polymorphism in several sites, capable of, especially, detecting the gene polymorphism in the several sites of cytochrome 2D6, 2C19, etc., with clearness and good repeatability, and to provide a reagent for the same. SOLUTION: This method for detecting the gene polymorphism comprises conducting amplification by using at least any one of a wild-type detecting oligonucleotide and a variant-type detecting oligonucleotide together with a nucleic acid-specific marker, wherein sequences having at least two variation sites different from each other are detected by an identical amplification condition. COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：要解决的问题：提供一种检测几个位点的基因多态性的方法，能够特别是检测细胞色素2D6,2C19等的几个位点的基因多态性，具有清晰和良好的重复性，并且提供同样的试剂。解决方案：用于检测基因多态性的方法包括使用野生型检测寡核苷酸和变体型检测寡核苷酸中的至少任一种与核酸特异性标记进行扩增，其中具有至少两个通过相同的放大条件检测彼此不同的变异位点。版权所有（C）2004，JPO＆NCIPI

38. 发明专利

JP2004180557A Method and reagent for simply detecting gene deletion of human cytochrome p4502d6 有权
公开(公告)号：JP2004180557A
公开(公告)日：2004-07-02
申请号：JP2002349813
申请日：2002-12-02
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , KITABAYASHI MASAO , TAKARADA YUTAKA
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a part or the whole of gene deletion, especially the gene deletion of human cytochrome P4502D6 clearly and in excellent reproduciblity, and to obtain a reagent therefor.
SOLUTION: The method for detecting a part or the whole of gene deletion comprises using a 2-Step PCR method. In the method for detecting the gene deletion, a part or the whole of the gene deletion is continuous 1,000 or more deletions. The method is suitably used for the gene deletion of the human cytochrome P4502D6.
COPYRIGHT: (C)2004,JPO&NCIPI

39. 发明专利

JP2014042459A Mycoplasma pneumonia detection method 有权
标题翻译： MYCOPLASMA PNEUMONIA检测方法
公开(公告)号：JP2014042459A
公开(公告)日：2014-03-13
申请号：JP2012184876
申请日：2012-08-24
申请人： Toyobo Co Ltd , 東洋紡株式会社
发明人： KAWASHIMA YOSUKE , SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for quickly performing detection of Mycoplasma pneumonia and/or a drug resistance test on Mycoplasma pneumonia.SOLUTION: This invention uses an oligonucleotide selected from the group consisting of consecutive 24-40 bases in a base sequence comprising a specific sequence, and an oligonucleotide primer set comprising a combination of oligonucleotides, comprising oligonucleotides selected from the group consisting of consecutive 24-40 bases in a base sequence comprising a specific sequence different from the above.
摘要翻译：要解决的问题：提供快速检测支原体肺炎和/或对支原体肺炎的耐药性试验的方法。解决方案：本发明使用选自下述碱基序列中的连续24-40个碱基的寡核苷酸，其包含特异性序列和包含寡核苷酸组合的寡核苷酸引物组，其包含选自包含与上述不同的特异性序列的碱基序列中的连续24-40个碱基的寡核苷酸。

40. 发明专利

JP2013048619A Primer and probe for detecting methicillin-resistant staphylococcus aureus, and method for detecting methicillin-resistant staphylococcus aureus using the same 有权
标题翻译：用于检测耐甲氧西林黄曲霉的前驱物和检测方法及使用该抗体的耐甲氧西林黄曲霉的检测方法
公开(公告)号：JP2013048619A
公开(公告)日：2013-03-14
申请号：JP2012172817
申请日：2012-08-03
申请人： Toyobo Co Ltd , 東洋紡株式会社
发明人： KAWASHIMA YOSUKE , SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide a method for accurately, rapidly and simply detecting MRSA (Methicillin-Resistant Staphylococcus Aureus).SOLUTION: The method for detecting MRSA in a sample includes detection of a complex including preparation of a pair of primers specifically-amplifying regions of a nuc gene specific to the staphylococcus aureus, amplification of the sample nucleic acid by a reaction liquid including the sample nucleic acid and the pair of nucleic acid primers, formation of a complex by hybridizing the nucleic acid amplification product with a nucleic acid probe designed to form a complex with a part of the nucleic acid amplification product, and detection of the product complex.
摘要翻译：要解决的问题：提供准确，快速和简单地检测MRSA（耐甲氧西林金黄色葡萄球菌）的方法。解决方案：检测样品中MRSA的方法包括检测复合物，包括制备特异性扩增金黄色葡萄球菌特异性核苷酸基因的区域的一对引物，通过反应液扩增样品核酸，包括样品核酸和一对核酸引物，通过将核酸扩增产物与设计成与核酸扩增产物的一部分形成复合物的核酸探针杂交并检测产物复合物来形成复合物。版权所有（C）2013，JPO＆INPIT

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