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21. 发明专利

JP2010130966A Cardiac muscle toxicity inspection device, cardiac muscle toxicity inspection chip and cardiac muscle toxicity inspection method 有权
标题翻译：心脏肌肉毒性检查装置，心脏肌肉毒性检查芯片和心脏肌肉毒性检查方法
公开(公告)号：JP2010130966A
公开(公告)日：2010-06-17
申请号：JP2008311341
申请日：2008-12-05
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , SUGIYAMA ATSUSHI , KANEKO SATOYUKI , NOMURA NORIMASA
IPC分类号： C12M1/34 , C12Q1/02 , G01N21/27 , G01N27/416 , G01N33/48
CPC分类号： G01N33/5014 , C12N2503/02 , G01N33/48728 , G01N33/5061
摘要： PROBLEM TO BE SOLVED: To provide a cardiac muscle toxicity inspection device which can equivalently perform a cardiac muscle toxicity inspection in vitro to that having been performed in vivo, and to provide a cardiac muscle toxicity inspection method. SOLUTION: A pulsation pacemaker cell mass 10 G is disposed on a transparent substrate 1, and cardiac muscle pulsation cells are then properly separately disposed. The suitable number of fibroblasts are arranged and bound between them to form a cell network. The cell network can optically be observed. The cells constituting the network are exposed to the flow of a liquid containing a medicine so that the medicine acts on the cells. A difference between the usual retardation of pulses from one of the cardiac muscle pulsation cells of the network to the final cardiac muscle pulsation cells and the retardation of pulses on the action of the medicine is caught with electric signals obtained from electrodes to evaluate Na + ion inhibition. One pulse of the cardiac muscle pulsation cells of the network is optically caught to detect the change of volume, thereby detecting a shrinkage speed on the action of the medicine to evaluate cardiac output. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种心脏毒性检查装置，其可以在体外等效地进行体内心肌毒性检查，并进行心肌毒性检查方法。解决方案：将脉动起搏器细胞块10S 设置在透明基片1上，然后适当地单独设置心肌搏动细胞。成纤维细胞的合适数目被布置和结合在一起形成细胞网络。可以光学地观察细胞网络。构成网络的细胞暴露于含有药物的液体的流动，使得药物作用于细胞。通过从电极获得的电信号捕获从网络的心肌搏动细胞到最终的心肌搏动细胞的脉冲的正常延迟与药物作用的脉冲延迟之间的差异，以评估Na + 离子抑制。网络的心肌脉搏细胞的一个脉冲被光学捕获以检测体积的变化，从而检测药物作用的收缩速度以评估心输出量。版权所有（C）2010，JPO＆INPIT

22. 发明专利

JP2009186280A Particle image measuring device 有权
标题翻译：颗粒图像测量装置
公开(公告)号：JP2009186280A
公开(公告)日：2009-08-20
申请号：JP2008025513
申请日：2008-02-05
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： TAKEDA KAZUO , HATTORI AKIHIRO , HAYASHI MASATO , YASUDA KENJI
IPC分类号： G01N15/02
摘要： PROBLEM TO BE SOLVED: To provide a particle image measuring device capable of enhancing the throughput of particle detection. SOLUTION: In this particle image measuring device in a fluid, a flow cell along which measuring object particles that flows downward are irradiated with a light from an LED which emits light, as long as a prescribed time when a shutter is opened, in synchronism with the clicking of the shutter of a camera for imaging an image of the particles. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供能够提高粒子检测的通过量的粒子图像测量装置。解决方案：在这种流体中的粒子图像测量装置中，只要在快门打开的规定时间内，只要从开口的LED发出的光照射着向下流动测量物体的流动池，与相机的快门的点击同步，以对颗粒的图像进行成像。版权所有（C）2009，JPO＆INPIT

23. 发明专利

JP2009128297A Method of forming minute particles, and inspection method of biological substance using the minute particles 有权
标题翻译：形成分钟颗粒的方法和使用分钟颗粒检测生物物质的方法
公开(公告)号：JP2009128297A
公开(公告)日：2009-06-11
申请号：JP2007305841
申请日：2007-11-27
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , KIN KENTETSU , TAKEI HIROYUKI
IPC分类号： G01N33/543 , B01J19/00 , C23C14/24 , G01N33/53 , G01N33/547 , G01N33/552 , G01N33/553
摘要： PROBLEM TO BE SOLVED: To provide a method of forming effectively modified minute particles and an improved inspection method of a biological substance that uses the minute particle as biological marker.
SOLUTION: A surface of a substrate for fixing the minute particles in a single layer is vapor-deposited with Au in a predetermined thickness. While, particle fixing liquid is manufactured by using materials for inhibiting static repulsive force between 1-ethyl-3(3-dimethylaminopropyl)carbodiimidehydrochloride (commonly called EDC), NaCl or KCl, and the like, and applied on the substrate with the particle suspension liquid made, by mixing the particles to the particle fixing liquid on the vapor deposited substrate surface with the prescribed thickness of Au, where the minute particles are fixed in a single layer. On the surface of the single layer of the fixed minute particle, transition metal, metal or semiconductor is vapor-deposited for forming the modified particles. For peeling the modified particles from the substrate, an ultrasonic cleansing device is used, by activating the ultrasonic waves to the substrate for setting forward the peeling. For the case where the modified particles is to be modified by the biological function molecules to be used as a marker for inspecting biologocal material, the reflection electrons from the modified particles are used.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种形成有效改性的微小颗粒的方法和改进使用微小颗粒作为生物标记物的生物物质的检查方法。解决方案：将用于将微小颗粒固定在单层中的基板的表面以预定厚度以Au气相沉积。同时，通过使用用于抑制1-乙基-3（3-二甲基氨基丙基）碳二亚胺盐酸盐（通常称为EDC），NaCl或KCl等之间的静止排斥力的材料制造颗粒定影液体，并且用颗粒悬浮液通过将颗粒与气相沉积基板表面上的颗粒定影液体以规定厚度的Au混合，其中微粒固定在单层中来制备液体。在固定的微小颗粒的单层的表面上，过渡金属，金属或半导体被气相沉积以形成改性颗粒。为了从基板剥离改性颗粒，通过将超声波激活到基板以设置剥离来使用超声波清洗装置。对于通过生物功能分子修饰改性粒子作为检测生物物质的标记物的情况，使用来自改性粒子的反射电子。版权所有（C）2009，JPO＆INPIT

24. 发明专利

JP2008237041A Method for selecting target cell specifically binding nucleic acid by annealing 审中-公开
标题翻译：通过退火选择目标细胞特异性结合核酸的方法
公开(公告)号：JP2008237041A
公开(公告)日：2008-10-09
申请号：JP2007078622
申请日：2007-03-26
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , ANZAI HISASHI , TERAZONO HIDEYUKI , FUKUSHIMA MAMORU
IPC分类号： C12Q1/68 , C12N15/09 , C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide a method for easily obtaining a DNA that specifically binds target cells, but not non-target cells.
SOLUTION: The target cell specifically binding DNA is obtained by selecting target cell specifically binding DNA candidates, selecting antisense strand DNAs of non-target cell specifically binding DNAs from the candidates and deleting the non-target cell specifically binding DNAs from the target cell specifically binding DNA candidates. Target cell specifically binding DNAs are determined for a plurality of target cells, the target cell specifically binding DNAs are contrasted for the two target cells and the coexisting DNAs are made DNAs specifically binding the final target cell.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种容易地获得特异性结合靶细胞而不是非靶细胞的DNA的方法。解决方案：特异性结合DNA的靶细胞通过选择靶细胞特异性结合DNA候选物，从候选物中选择非靶细胞特异性结合DNA的反义链DNA，并从目标中删除特异性结合DNA的非靶细胞细胞特异性结合DNA候选物。针对多个靶细胞确定特异性结合DNA的靶细胞，对于两个靶细胞对比特异性结合DNA的靶细胞，并且使共存的DNA成为特异性结合最终靶细胞的DNA。版权所有（C）2009，JPO＆INPIT

25. 发明专利

JP2008237073A Apparatus for separating cell 审中-公开
标题翻译：分离细胞的装置
公开(公告)号：JP2008237073A
公开(公告)日：2008-10-09
申请号：JP2007080579
申请日：2007-03-27
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , HATTORI AKIHIRO , FUKUSHIMA MAMORU
IPC分类号： C12M3/00 , C12N5/07 , C12N5/09
摘要： PROBLEM TO BE SOLVED: To separate cells by detecting photons emitted by the cells utilizing a cell sorter using micro-flow channels formed on a substrate. SOLUTION: A compound labeled with a positron-detay nuclide in a human body is used as a radioactive tracer in positron tomography. Attention is paid to gathering of the administered tracer in a site such as cancer or tumor in an organ of the human body to obtain the cells from a sample from the site. Thus, cell separation is carried out with the cell sorter using the micro-flow channels. A photoelectronic detector is arranged in close vicinity to a cell detecting region to send a signal from a computer to a cell separating region according to a photon detecting signal with the photoelectronic detector to separate the cells. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：通过使用形成在衬底上的微流通道，利用细胞分选器检测细胞发射的光子来分离细胞。解决方案：在正电子断层摄影中，将用人体中的正电子 - 核素核素标记的化合物用作放射性示踪剂。注意将所施用的示踪剂收集在人体器官中的癌症或肿瘤等位点，从现场的样品中获取细胞。因此，细胞分离使用微流通道进行细胞分选。光电子检测器被布置在细胞检测区域附近，以便根据光电检测信号从计算机将信号发送到细胞分离区域以分离细胞。版权所有（C）2009，JPO＆INPIT

26. 发明专利

JP2009118798A Apparatus and method for amplifying nucleic acid, and method of culturing cell and amplifying nucleic acid 有权
标题翻译：放大核酸的装置和方法，以及培养细胞和放大核酸的方法
公开(公告)号：JP2009118798A
公开(公告)日：2009-06-04
申请号：JP2007297542
申请日：2007-11-16
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： TERAZONO HIDEYUKI , HATTORI AKIHIRO , YASUDA KENJI
IPC分类号： C12M1/00
摘要： PROBLEM TO BE SOLVED: To provide a method for amplifying a nucleic acid in a trace quantity.
SOLUTION: The apparatus and the method for amplifying nucleic acid in trace quantity, in real time are provided. The method for culturing cell and amplifying the nucleic acid in real time can carry out steps, starting from the step of the cell culturing to the step of real-time nucleic acid amplification, using the same apparatus.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供微量扩增核酸的方法。解决方案：提供了实时放大微量核酸的装置和方法。培养细胞并实时扩增核酸的方法可以使用相同的装置从细胞培养步骤开始到实时核酸扩增步骤。版权所有（C）2009，JPO＆INPIT

27. 发明专利

JP2009036694A Method for analyzing biological substance in cell maintaining spatial distribution 审中-公开
标题翻译：在细胞维持空间分布中分析生物物质的方法
公开(公告)号：JP2009036694A
公开(公告)日：2009-02-19
申请号：JP2007202560
申请日：2007-08-03
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , KIN KENTETSU , FUKUSHIMA MAMORU
IPC分类号： G01N27/447 , C12N15/09 , C12Q1/02 , G01N1/28 , G01N23/225
摘要： PROBLEM TO BE SOLVED: To provide a method for quantitatively measuring mRNA and protein localized between cells and in cells without losing localization space information. SOLUTION: A cell or a sample flake is held between bar-like isoelectric focusing gels of predetermined thickness to carry out electrophoresis. The mRNA and protein localized between cells and in cells can thereby be moved in the isoelectric focusing gels to positions corresponding to their pK values while maintaining a state of distribution of a two-dimensionally projected form. The bar-like isoelectric focusing gels are then cut off in thin film shape in the positions corresponding to the pK values, and contents in the position are examined to carry out quantitative measurement without losing localization space information. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供定量测量细胞和细胞之间的mRNA和蛋白质的方法，而不会损失定位空间信息。解决方案：将细胞或样品片保持在预定厚度的棒状等电聚焦凝胶之间进行电泳。因此，定位在细胞和细胞之间的mRNA和蛋白质可以在等电聚焦凝胶中移动到与其pK值相对应的位置，同时保持二维投影形式的分布状态。然后将棒状等电聚焦凝胶在与pK值相对应的位置上以薄膜形状切断，并且检查位置中的内容以进行定量测量而不失去定位空间信息。版权所有（C）2009，JPO＆INPIT

28. 发明专利

JP2008301805A Chip and device for purifying cells, and chip and device for separating cells and fine particles 审中-公开
标题翻译：用于净化细胞的芯片和装置，以及用于分离细胞和细颗粒的芯片和装置
公开(公告)号：JP2008301805A
公开(公告)日：2008-12-18
申请号：JP2007202564
申请日：2007-08-03
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： YASUDA KENJI , HATTORI AKIHIRO , FUKUSHIMA MAMORU
IPC分类号： C12M1/00
CPC分类号： C12M47/04
摘要： PROBLEM TO BE SOLVED: To provide a chip and device for purifying cells, capable of purifying the cells by using a flow passage formed on a substrate plate and which separate the cells and fine particles. SOLUTION: This device for purifying the cells is provided, having a flow passage that has a function of forming a stratified flow and flowing the cells as a row by taking a certain distance in its center; a means for observing and discriminating the cells in the flow passage; 3 or larger small recesses formed at the side of the flow passage and a gel electrode (electroconductive agarose layer), introduced to the bottom surface part of each of the recesses, and introducing the cell as a single unit into the recess, by giving a potential selectively corresponding to the difference of kinds of the cells to each of the agarose layers. The cells, introduced in the recess, are put into the flowing passage selectively, by giving a reverse potential to that given at the introduction, to each of the agarose layers. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于净化细胞的芯片和装置，其能够通过使用形成在基板上的流路来净化细胞并分离细胞和细颗粒。解决方案：提供这种用于净化电池的装置，具有流路，其具有形成分层流的功能，并且通过在其中心一定距离使电池作为一排流动; 用于观察和区分流动通道中的细胞的装置; 在流路侧形成的3个以上的小凹部和引入到各凹部的底面部的凝胶电极（导电琼脂糖层），将该单元作为一个单元导入到凹部中，选择性地对应于细胞的种类与每个琼脂糖层的差异的电位。引入到凹部中的细胞通过给予每个琼脂糖层的选择性地将引入时给出的反应电位选择性地放入流动通道中。版权所有（C）2009，JPO＆INPIT

29. 发明专利

JP2008283883A Method for selection of nucleic acid specifically binding to target protein by hybridization 审中-公开
标题翻译：通过杂交选择核酸特异性结合靶向蛋白的方法
公开(公告)号：JP2008283883A
公开(公告)日：2008-11-27
申请号：JP2007130257
申请日：2007-05-16
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： ANZAI HISASHI , YASUDA KENJI , TERAZONO HIDEYUKI , FUKUSHIMA MAMORU
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method by which a DNA specifically binding to a target protein without binding to a non-target protein is readily obtained.
SOLUTION: The method comprises selecting DNA candidates specifically binding to the target protein, selecting an anti-sense chain DNA of the DNA specifically binding to the non-target protein among the candidates, removing the DNA specifically binding to the non-target protein from the DNA candidates specifically binding to the target protein and determining the DNA specifically binding to the target protein.
COPYRIGHT: (C)2009,JPO&INPIT

30. 发明专利

JP2008278791A Nucleic acid amplification apparatus, method, cell culturing, and nucleic acid amplification method 审中-公开
标题翻译：核酸扩增装置，方法，细胞培养和核酸扩增方法
公开(公告)号：JP2008278791A
公开(公告)日：2008-11-20
申请号：JP2007125472
申请日：2007-05-10
申请人： Tokyo Medical & Dental Univ , 国立大学法人東京医科歯科大学
发明人： TERAZONO HIDEYUKI , YASUDA KENJI , FUKUSHIMA MAMORU
IPC分类号： C12N15/09 , C12M1/00 , C12M3/00
摘要： PROBLEM TO BE SOLVED: To provide an apparatus and a method for amplifying a very small amount of nucleic acid.
SOLUTION: A real-time nucleic acid amplification apparatus comprises a chip equipped with a plurality of transparent electrodes, in a belt-like state, having a function of maintaining a substrate temperature constant while measuring the temperature from 50°C to 100°C on the surface thereof; a mineral oil layer formed on the chip; a means for introducing water droplets to the mineral oil layer; a means for measuring the change of fluorescence intensity of water droplets; and a means for irradiating the water droplets with a converging light at a wavelength having water absorption and heating the water droplets.
COPYRIGHT: (C)2009,JPO&INPIT

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