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21. 发明专利

JP2007300921A Method for inserting sequence element into nucleic acid 有权
标题翻译：将序列元素插入核酸的方法
公开(公告)号：JP2007300921A
公开(公告)日：2007-11-22
申请号：JP2007121557
申请日：2007-05-02
申请人： Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング
发明人： KORFHAGE CHRISTIAN , ENGEL HOLGER , LOEFFERT DIRK , PEIST RALF
IPC分类号： C12N15/09
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6816 , C12Q2563/179 , C12Q2525/161 , C12Q2521/101
摘要： PROBLEM TO BE SOLVED: To provide a method for inserting one or more tag sequences into a nucleic acid. SOLUTION: The method for inserting one or more tag sequences into a nucleic acid comprises (a) a step to prepare a template nucleic acid, (b) a step to hybridize at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid, and (c) a step to synthesize a new strand of nucleic acid partially complementary to the template nucleic acid and containing, on its 3'-terminal, a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供将一个或多个标签序列插入核酸的方法。解决方案：将一个或多个标签序列插入核酸的方法包括（a）制备模板核酸的步骤，（b）将至少一种锚定寡核苷酸的至少一个锚定序列与模板核酸的一个序列部分，和（c）合成与模板核酸部分互补的核酸的新链，并在其3'末端含有与非杂交部分互补的序列的步骤锚定寡核苷酸，例如至少一个标签序列。版权所有（C）2008，JPO＆INPIT

22. 发明专利

JP2000342259A STABILIZATION AND/OR ISOLATION OF NUCLEIC ACID 失效
公开(公告)号：JP2000342259A
公开(公告)日：2000-12-12
申请号：JP2000045524
申请日：2000-02-23
申请人： QIAGEN GMBH
发明人： ERBACHER CHRISTOPH , BASTIAN HELGE , WYRICH RALF , OELMUELLER UWE , MANZ THOMAS
IPC分类号： G01N33/50 , A61K31/14 , A61K31/495 , A61K31/7088 , C07C211/63 , C07D295/02 , C07H1/00 , C07H1/06 , C07H21/02 , C07H21/04 , C12N15/09 , C12N15/10 , C12Q1/00 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To obtain a medicine composition, a diagnosis composition and a study composition by bringing a specific cationic compound into contact with a biological sample, stabilizing a nucleic acid from the biological sample and isolating the nucleic acid. SOLUTION: A base bonded to a strong and/or weak inorganic and/or organic acid is used as an anion. A cationic compound in which a substance comprising a cationic compound and an anion is totally neutral and which is one or more cationic compounds of the formula [X is N or P; k is 1-24; Bk is a (substitute) aliphatic alkanediyl crosslinking, substituted phenyl, naphthyl or biphenyl crosslinking; R1, R2 and RBk are H, a (substitute) 1-6C alkyl, phenyl or the like; RA, RBk and RC are each H, a (substitute) 1-21C alkyl, a 1-21C alkenyl or the like] such as ethanediyl-1,2-bis(dimethyldecylammonium bromide) is brought into contact with a biological sample such as clinical sample, for example, blood, etc., to stabilize and/or isolate a nucleic acid from the biological sample.

23. 发明专利

JP2015037427A アンカーオリゴヌクレオチドおよびアダプターオリゴヌクレオチドを用いる核酸の正規化した定量化方法审中-公开
标题翻译：使用锚定寡核苷酸和适配子寡核苷酸对核酸进行正规化定量的方法
公开(公告)号：JP2015037427A
公开(公告)日：2015-02-26
申请号：JP2014229735
申请日：2014-11-12
申请人：キアゲンゲーエムベーハー , Qiagen Gmbh , キアゲンゲーエムベーハー
发明人： LOEFFERT DIRK , KORFHAGE CHRISTIAN , ENGEL HOLGER
IPC分类号： C12Q1/68 , C12N15/00 , C12N15/09
CPC分类号： C12Q1/6851 , C12Q2545/101 , C12Q2525/173 , C12Q2563/179 , C12Q2561/101
摘要：【課題】アンカーオリゴヌクレオチドおよびアダプターオリゴヌクレオチドを用いる核酸の正規化した定量化方法の提供。【解決手段】本発明は、核酸の正規化された定量化、および試料、例えば、核酸の混合物における核酸の数量についての正規化に関する。本発明は、試料中の定量化される核酸の数量を、試料中の核酸の総数量、または試料中の特定のクラスの核酸の総数量に対して正規化するための方法に関する。本発明は、試料中の核酸の正規化された定量化のためのキットであって、試料中の核酸の限定された領域または特定のクラスの核酸と実質的に相補的な1種以上の第1の核酸プローブであって、1種以上のプライマー結合部位および1つのプローブ結合部位を含む第1の核酸プローブ;ならびに前記第1の核酸プローブの前記プローブ結合部位と実質的に相補的な第2の核酸プローブを含むキットにも関する。【選択図】図1
摘要翻译：要解决的问题：提供使用锚定寡核苷酸和接头寡核苷酸对核酸进行归一化定量的方法。解决方案：本发明涉及核酸的归一化定量和样品中核酸量的归一化，例如，核酸混合物。本发明涉及将样品中待定量的核酸的量归一化为样品中核酸总量的方法; 或相对于样品中特定类别的核酸的总量。本发明还涉及用于样品中的核酸的归一化定量的试剂盒，所述试剂盒包含：与样品中的所述核酸或特定类型核酸基本上互补的一个或多个第一核酸探针，其中第一核酸探针包含一个或多个引物结合位点和探针结合位点; 和与第一核酸探针上的探针结合位点基本上互补的第二核酸探针。

24. 发明专利

JP2014041132A Method for processing biological sample 有权
标题翻译：处理生物样本的方法
公开(公告)号：JP2014041132A
公开(公告)日：2014-03-06
申请号：JP2013173652
申请日：2013-08-23
申请人： Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング
发明人： HOLLAENDER VERA
IPC分类号： G01N1/28 , C12M1/00 , C12Q1/68
CPC分类号： B01L3/508 , B01L2300/046 , G01N1/30 , G01N1/31 , Y10T436/107497 , Y10T436/108331 , Y10T436/2525
摘要： PROBLEM TO BE SOLVED: To provide a method for stably storing a biological sample at room temperature.SOLUTION: A biological sample 1 is immersed, mixed and kept in a composition containing at least one polyol with maximum 100 wt.% and at least one additive with 0 to 99% wt.%. A container with a lid provided with an immersion auxiliary tool 3 and a mixed device 4 such as a container 1 is used for sample preparation and storage. A biology tissue sample prepared by this method can be subjected to histological analysis, nucleic acid analysis, and protein analysis even after being kept under moderate conditions, such as at room temperature or on a cold storage.
摘要翻译：要解决的问题：提供一种在室温下稳定存储生物样品的方法。解决方案：将生物样品1浸入，混合并保持在含有至少一种最多100重量％的多元醇和至少一种添加剂的组合物中 0至99重量％。具有设置有浸没辅助工具3的盖的容器和诸如容器1的混合装置4用于样品制备和储存。通过该方法制备的生物组织样品即使在温和条件下，例如在室温或冷藏下也可进行组织学分析，核酸分析和蛋白质分析。

25. 发明专利

JP2010279374A Method for isolating dna from biological material 审中-公开
标题翻译：从生物材料中分离DNA的方法
公开(公告)号：JP2010279374A
公开(公告)日：2010-12-16
申请号：JP2010165082
申请日：2010-07-22
申请人： Max Planck G Zur Foerderung Wissenschaft Ev , Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング , マックス−プランク−ゲゼルシャフトツールフェルデルンクデルヴィッセンシャフテンエー.ファウ.
发明人： MUELLER OLIVER , SPRENGER-HAUSSELS MARKUS , BASTIAN HELGE , VOLLERT STEFANIE
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： PROBLEM TO BE SOLVED: To provide a reagent kit suitable for carrying out a method designed for stabilizing, purifying and/or isolating a nucleic acid from biological materials, especially from stool samples that can contain impurities and interfering substances.
SOLUTION: The method comprises purifying, stabilizing and/or isolating a nucleic acid from biological materials wherein an adsorption matrix (an insoluble carbohydrate-based adsorption matrix) in order to bind impurities thereto and an extraction buffer are added to a nucleic acid-containing sample, followed by removing the nucleic acid from the adsorption matrix, and wherein the extraction buffer has: (a) a 2-8 pH range; and (b) salt concentration of at least 100 mM, and/or contains (c) a phenol-neutralizing substance.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供适于实施设计用于从生物材料，特别是可含有杂质和干扰物质的粪便样品中稳定，纯化和/或分离核酸的试剂盒。解决方案：该方法包括从生物材料中纯化，稳定和/或分离核酸，其中将杂质与其结合的吸附基质（不溶性碳水化合物基吸附基质）和提取缓冲液加入到核酸然后从吸附基质中除去核酸，其中提取缓冲液具有：（a）2-8个pH范围; 和（b）盐浓度至少为100mM，和/或含有（c）苯酚中和物质。版权所有（C）2011，JPO＆INPIT

26. 发明专利

JP2010158250A Nucleic acid amplification 审中-公开
标题翻译：核酸放大
公开(公告)号：JP2010158250A
公开(公告)日：2010-07-22
申请号：JP2010042086
申请日：2010-02-26
申请人： Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング
发明人： LASKEN ROGER S , EGHOLM MICHAEL , ALSMADI OSAMA A , BORNARTH CAROLE , WISNIEWSKI MICHELE , HOSONO SEIYU , RAGHUNATHAN ARUMUGHAM
IPC分类号： C12N15/09 , C07H21/04 , C12P19/34 , C12Q20060101 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2531/119
摘要： PROBLEM TO BE SOLVED: To provide compositions and methods for amplification of nucleic acid sequences of interest. SOLUTION: The method comprises incubating nucleic acids including the sequence of interest, at a high temperature, in the presence of heat-unstable nucleic acid polymerase having strand substituting activity, additives and a pair of primers and under conditions promoting replication of the nucleic acids, wherein the replication of the nucleic acid produces the replicated strands as the result, at least one of the replicated nucleic acid strands is substituted by strand substitution replication of another replicated strand, formation of the strands replicated from the sequences of interest is more advantageous than that of strands replicated from the sequences of no interest. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供用于扩增感兴趣的核酸序列的组合物和方法。解决方案：该方法包括在高温下，在具有链取代活性的热不稳定核酸聚合酶存在下孵育包括所述序列的核酸，添加剂和一对引物，并且在促进核酸，其中核酸的复制作为结果产生复制的链，所述复制的核酸链中的至少一个被另一个复制链的链取代复制所取代，从感兴趣的序列复制的链的形成更多优于从不感兴趣的序列复制的链的优势。版权所有（C）2010，JPO＆INPIT

27. 发明专利

JP2007111048A Method for polymerase chain reaction to use dna polymerase having proofreading property 有权
标题翻译：聚合酶链反应使用具有防腐性能的DNA聚合酶的方法
公开(公告)号：JP2007111048A
公开(公告)日：2007-05-10
申请号：JP2006273598
申请日：2006-10-05
申请人： Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング
发明人： ENGEL HOLGER , PEIST RALF
IPC分类号： C12N15/09
CPC分类号： C12Q1/686 , C12Q2525/113 , C12Q2525/125 , C12Q2525/179 , C12Q2525/186 , C12Q2527/125 , C12Q2521/319 , C12Q2521/101
摘要： PROBLEM TO BE SOLVED: To provide a method for a polymerase chain reaction to use a template nucleic acid, at least one primer, deoxyribonucleoside triphosphates and a polymerase having proofreading activity. SOLUTION: The invention provides a method for significantly increasing the efficiency of a DNA polymerase having proofreading activity and/or reducing or, in the optimal case, blocking the 3'-5'-exonuclease activity of the DNA polymerase. The target substrate used in the invention is in particular single stranded, linear oligonucleotides, hairpin oligonucleotides and RNA and DNA molecules. Furthermore, the invention discloses a kit containing a reagent necessary for the implementation of the polymerase chain reaction. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供聚合酶链式反应使用模板核酸，至少一种引物，脱氧核糖核苷三磷酸和具有校对活性的聚合酶的方法。解决方案：本发明提供了一种显着提高具有校对活性的DNA聚合酶的效率和/或减少或在最佳情况下阻断DNA聚合酶的3'-5'-外切核酸酶活性的方法。用于本发明的靶基质特别是单链，线性寡核苷酸，发夹寡核苷酸，RNA和DNA分子。此外，本发明公开了一种包含实施聚合酶链式反应所必需的试剂的试剂盒。版权所有（C）2007，JPO＆INPIT

28. 发明专利

JP2004113242A Enhanced coamplification of nucleic acid 有权
公开(公告)号：JP2004113242A
公开(公告)日：2004-04-15
申请号：JP2003332155
申请日：2003-09-24
申请人： Qiagen Gmbh , キアゲンゲゼルシャフトミットベシュレンクテルハフツング
发明人： ENGEL HOLGER , LOEFFERT DIRK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q2537/143
摘要： PROBLEM TO BE SOLVED: To provide a method and a reagent for increasing efficiency of coamplification reactions such as multiplex PCR (polymerase chain reaction) reaction. SOLUTION: The efficient conditions for multiplex PCR amplification includes using hot start DNA polymerase with a volume exclusion agent in PCR reaction. The positive effect for multiplex PCR is based on, on the one hand, use of a hot start enzyme maximizing PCR efficiency by minimizing undesired nonspecific hybridization and extension of primer and formation of duplex primer. On the other hand, the existence of the volume exclusion agent improves hybridization speed of all reacting primer. By existence of the reacting volume exclusion agent, product yield of a target exhibiting little amplification under a multiplex PCR conditions (defined primer concentration) is much increased. COPYRIGHT: (C)2004,JPO

29. 发明专利

JP2000093171A NEW METHOD FOR PURIFYING CONVALENTLY CLOSED CIRCULAR DNA 失效
公开(公告)号：JP2000093171A
公开(公告)日：2000-04-04
申请号：JP26032799
申请日：1999-09-14
申请人： QIAGEN GMBH
发明人： WAHLE STEPHAN , SCHORR JOACHIM , WEBER MARTIN DR
IPC分类号： C12N15/00 , A61K31/7088 , A61K48/00 , C12N15/09 , C12N15/10 , C12P19/34 , C12Q1/34 , C12R1/19 , G01N30/88
摘要： PROBLEM TO BE SOLVED: To provide a method for reducing the subject circular DNA by precipitating cleared lysate of a unicellular organism with a specific volume of alcohol, followed by washing and resuspending, digesting genomic DNA with a nuclease and bringing the resultant product into contact with an anion-exchanging substance. SOLUTION: This method is such one as to produce a covalently closed circular(ccc) DNA practically containing no genomic DNA from a unicellular organism. The covalently closed circular DNA, such as a plasmid DNA is obtained by precipitating cleared lysate of the unicellular organism with alcohol at 0.6-5 vol. times the lysate, washing the precipitate with an alcoholic solution, resuspending the precipitate, digesting the genomic DNA with a nuclease cleaving linear DNA and/or circular DNA having a nick or free 3' or 5'-terminal and not being cccDNA, bringing the resultant product into contact with an anion-exchanging substance to separate purified cccDNA from the residue of the product.

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