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11. 发明专利

JP5620382B2 ＲＮＡの完全性の測定有权
标题翻译： RNA的完整性测量
公开(公告)号：JP5620382B2
公开(公告)日：2014-11-05
申请号：JP2011525270
申请日：2009-08-31
IPC分类号： G01N27/26 , C12M1/00 , C12Q1/68 , G01N27/447
CPC分类号： C12Q1/6816 , C12Q1/6806 , C12Q2545/113 , C12Q2563/107 , C12Q2565/125 , G01N2333/4728 , G01N2800/34 , C12Q2561/12 , C12Q2539/101

12. 发明专利

JP2013537806A Apparatus and method for assessing the characteristics of the target molecule 有权
公开(公告)号：JP2013537806A
公开(公告)日：2013-10-07
申请号：JP2013529581
申请日：2011-09-27
申请人：テヒニシェウニヴェルズィテートミュンヘンTechnische Universitaet Muenchen
发明人：ウルリッヒ、ラント , ボルフガング、カイザー , ポール、アンドレアス、ハンペル , イェンス、ニーマックス , アンドレアス、ランガー , イェレナ、クネツェビク
IPC分类号： C12M1/00 , C12Q1/68
CPC分类号： G01N27/44726 , C12Q1/6823 , C12Q1/6825 , G01N21/6408 , G01N21/6458 , G01N27/327 , G01N27/44756 , G01N33/5438 , G01N2021/6432 , C12Q2537/165 , C12Q2561/12 , C12Q2563/116
摘要： Disclosed herein are an apparatus (20) and a method for evaluating one or more characteristics of target molecules. The apparatus comprises means (22) for receiving a biochip comprising a substrate (16) to which charged probe molecules (10) are attached. The probe molecules have a marker (12) for allowing to generate signals indicative of the distance of a portion of the probe molecule (10) from the substrate (16). The apparatus comprises means (34, 38 - 44) for detecting the signal and means (28, 29) for generating an external electric field to which the probe molecules (10) are exposed. A control means controls the electric field generating means (28) to (A) apply an external electric field causing the portion of the probe molecule (10) to approach said substrate (16) and (B) apply an external electric field causing the portion of the probe molecule (10) to move away from said substrate (16). The signal detecting means (34, 38 - 44) records the signal as a function of time during step (A) and/or step (B). Steps (A) and (B) are repeated for a predetermined number of times and the recorded signals are combined such as to generate an averaged time-resolved signal indicative of the process of said part of the probe molecule (10) approaching the substrate (16) and/or moving away from the substrate (16).

13. 发明专利

JP2010505440A The double-stranded probe for fluorescence detection of nucleic acid 有权
公开(公告)号：JP2010505440A
公开(公告)日：2010-02-25
申请号：JP2009531936
申请日：2007-10-12
申请人：ビオ−ラド・パストゥールBio−Rad Pasteur
发明人：ティファニー・ダグラン , フランソワ・リウニエ
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/78
CPC分类号： C12Q1/706 , C12Q1/6818 , C12Q1/6844 , C12Q1/703 , C12Q2565/525 , C12Q2537/161 , C12Q2525/161 , C12Q2561/12 , C12Q2561/113 , C12Q2531/113
摘要：本発明は、少なくとも一つの一本鎖または二本鎖標的核酸の蛍光検出を目的とする二本鎖プローブであって、該二本鎖プローブが、式Ｔ'
１ −Ｔ
１の配列を含む標的核酸の第一の鎖の検出を目的とする、式Ｘ
１ −（Ｌ
１）
ａ −Ｓ
１ −Ｓ'
１ −（Ｌ'
１）
ｂ −Ｙ
１の第一の鎖；標的核酸の第二の鎖が存在する場合に、式Ｔ'
２ −Ｔ
２の配列を含む標的核酸の第二の鎖の検出を目的とする、式Ｘ
２ −（Ｌ
２）
ｃ −Ｓ
２ −Ｓ'
２ −（Ｌ'
２）
ｄ −Ｙ
２の第二の鎖を含み、ここで、Ｘ
１、Ｘ
２、Ｙ
１およびＹ
２のうち二つは蛍光供与体であり、一方で、他の二つは蛍光受容体であり、また、Ｘ
１およびＹ
２が共に蛍光供与体であることはできない、二本鎖プローブに関する。

14. 发明专利

JP2003325200A New high-sensitive method for assaying nucleic acid 审中-公开
标题翻译：用于测定核酸的新型高灵敏度方法
公开(公告)号：JP2003325200A
公开(公告)日：2003-11-18
申请号：JP2003061958
申请日：2003-03-07
申请人： Kazuko Matsumoto , 和子松本
发明人： MATSUMOTO KAZUKO , EN HIROTOSHI
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/533
CPC分类号： C12Q1/6818 , C12Q1/6827 , C12Q2563/137 , C12Q2561/12 , C12Q2561/109
摘要： PROBLEM TO BE SOLVED: To provide a high-sensitive method for assaying SNP, specifically to provide the method for assaying the SNP by which a large amount of the SNP is rapidly assayed by a small amount of a DNA of a test object. SOLUTION: The method for assaying the SNP by using an invader method using a FRET (Fluorescence Resonance Energy Transfer) probe having a luminous coloring matter and a quenching material uses a labeling agent of a rare earth fluorescent complex comprising a rare earth element such as europium and terbium as the luminous coloring matter in the FRET probe. The fluorescence-regulating composition comprises a combination of the specific labeling agent of the rare earth fluorescent complex and the specific labeling agent of a fluorescence quencher. The high-sensitive labeling probe uses the labeling agent of the rare earth fluorescent complex as the luminous coloring matter in the high-sensitive labeling probe such as the FRET probe, and the labeling agent of the fluorescence quencher as the quenching material. The kit for assaying the SNP includes the high-sensitive labeling probe. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供用于测定SNP的高灵敏度方法，特别是提供用于测定SNP的方法，其中通过少量测试对象的DNA快速测定大量的SNP 。解决方案：使用具有发光色素和猝灭材料的FRET（荧光共振能量转移）探针的入侵者方法测定SNP的方法使用包含稀土元素的稀土荧光复合物的标记试剂如铕和铽作为FRET探针中的发光色素。荧光调节组合物包含稀土荧光复合物的特异性标记试剂和荧光猝灭剂的特异性标记试剂的组合。高灵敏度标记探针使用稀土荧光复合物的标记剂作为高灵敏度标记探针中的发光色素，如FRET探针，荧光猝灭剂的标记试剂作为淬火材料。用于测定SNP的试剂盒包括高灵敏度的标记探针。版权所有（C）2004，JPO

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