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11. 发明专利

JP2005130730A Cell disrupter and, method for disrupting cell 审中-公开
标题翻译：细胞破坏和破坏细胞的方法
公开(公告)号：JP2005130730A
公开(公告)日：2005-05-26
申请号：JP2003368172
申请日：2003-10-28
申请人： Hitachi Koki Co Ltd , 日立工機株式会社
发明人： NAGAOKA HITOSHI , YAMADA KENJI , MORITA MASATAKA , NAGATA AKIO , KAMATAKI TETSUYA
IPC分类号： C12M1/33 , B02C18/14 , B02C18/16 , C12N1/06
CPC分类号： C12M45/02 , C12M47/08 , C12M47/20
摘要： PROBLEM TO BE SOLVED: To provide a cell disrupter which can simply, quickly and perfectly freeze and disrupt tissue pieces including the liver, heart, and smooth muscle of an experimental animal or the like in high useful substance recovery and in good disruption efficiency.
SOLUTION: This cell disrupter that a screw shaft driven with a motor is rotatably supported in a receiving cylinder having a feeding port and that a fixed blade and a rotary blade for disrupting biological tissue pieces fed with the screw shaft are attached to the discharging side of the receiving cylinder is characterized by disposing a cooling tank for freezing the biological tissue pieces at a place near to the feeding port. The method for disrupting the cells is characterized by feeding frozen biological tissue pieces or the frozen biological tissue pieces and ice in a mixed state into a feeding port.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种细胞破坏器，其可以在高有用物质回收和良好破坏中简单，快速和完美地冻结和破坏包括实验动物等的肝，心脏和平滑肌的组织块效率。解决方案：该电池破坏器，用电动机驱动的螺杆轴可旋转地支撑在具有进料口的容纳圆筒中，并且固定刀片和用于破坏供给螺杆轴的生物组织片的旋转刀片被附接到容纳筒的排出侧的特征在于，在靠近进料口的位置设置用于冷冻生物组织片的冷却槽。破坏细胞的方法的特征在于将冷冻的生物组织块或冷冻的生物组织块和冰以混合状态送入进料口。版权所有（C）2005，JPO＆NCIPI

12. 发明专利

JPS6143982A Continuous flow homogenizer 失效
标题翻译：连续流动均质机
公开(公告)号：JPS6143982A
公开(公告)日：1986-03-03
申请号：JP16421584
申请日：1984-08-07
申请人： Yamato Scient Co Ltd
发明人： SAITO TATSUHIKO , TAKAHATA HIKARI
IPC分类号： B02C19/10 , B01F15/00 , C12M1/00 , C12M1/33
CPC分类号： C12M47/08
摘要： PURPOSE: To obtain the titled homogenizer having high disintegration efficiency, and operable easily, by placing a pestle in a tubular vessel, and jointing the rotary shaft of the pestle detachably to the driving shaft with a one-way clutch.
CONSTITUTION: A pestle 13 is placed rotatably in a tubular vessel 9 interposing small gap between the inner wall of the vessel. The rotary shaft 11 of the pestle 13 is connected to the driving mechanism 15 in a vertically detachable manner with a one-way clutch 19 which makes the rotary shaft 11 rotatable in a nearly horizontal plane in one direction. The unemulsified tissue poured into the vessel through the inlet 5 is emulsified in the space between the outer circumference of the pestle and the inner wall of the tubular vessel, and is discharged continuously from the outlet port 7.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：为了获得具有高崩解效率的标准均化器，并且容易操作，通过将杵放置在管状容器中，并且用单向离合器将杵的旋转轴可拆卸地连接到驱动轴上。构成：研杵13可旋转地放置在管状容器9中，管状容器9在容器的内壁之间插入小间隙。杵13的旋转轴11通过单向离合器19以可垂直拆卸的方式与驱动机构15连接，该单向离合器19使旋转轴11沿一个方向在大致水平面上旋转。通过入口5倒入容器中的未乳化组织在杵的外周和管状容器的内壁之间的空间中被乳化，并且从出口7连续排出。

13. 发明专利

JP2017529064A 超高圧均質一体化装置及び細胞破砕器审中-公开
公开(公告)号：JP2017529064A
公开(公告)日：2017-10-05
申请号：JP2017503166
申请日：2015-03-31
申请人： ▲廣▼州聚能▲納▼米生物科技股▲分▼有限公司Guangzhou Juneng Nano&Biotechnology Co.,Ltd. , ▲廣▼州聚能▲納▼米生物科技股▲分▼有限公司Guangzhou Juneng Nano&Biotechnology Co.,Ltd.
发明人：余行文 , 余茜
IPC分类号： C12M1/33
CPC分类号： C12M47/06 , C12M23/34 , C12M27/00 , C12M29/14 , C12M33/12 , C12M41/40 , C12M45/02 , C12M47/08 , C12N1/066 , F04B9/113 , F04B31/00
摘要：【解決手段】本発明は、長尺シリンダ51と、主連結スリーブ52と、高圧シリンダ均質化本体53と、副連結スリーブ54と、短尺シリンダ55とを備えており、前記高圧シリンダ均質化本体の上部には高圧チャンバに連通する供給口が設けられており、前記供給口には一体化供給装置57が接続される超高圧均質一体化装置5である。前記長尺シリンダ、主連結スリーブ、高圧シリンダ均質化本体、副連結スリーブ及び短尺シリンダは順次、同軸心線で取り付けられるとともに、高圧シリンダ均質化本体の高圧チャンバ内の加圧プランジャ531が長尺シリンダのピストンロッドに接続されており、前記高圧シリンダ均質化本体における高圧チャンバに連通するチャンバ内の均質化バルブが短尺シリンダのイジェクタピン532に接続される、超高圧均質一体化装置及び細胞破砕器を開示する。【選択図】図3

14. 发明专利

JP2017527270A 同軸線ダブル高圧シリンダ均質一体化装置审中-公开
公开(公告)号：JP2017527270A
公开(公告)日：2017-09-21
申请号：JP2017503167
申请日：2015-03-31
申请人： ▲廣▼州聚能▲納▼米生物科技股▲分▼有限公司Guangzhou Juneng Nano&Biotechnology Co.,Ltd. , ▲廣▼州聚能▲納▼米生物科技股▲分▼有限公司Guangzhou Juneng Nano&Biotechnology Co.,Ltd.
发明人：余行文 , 余茜
IPC分类号： C12M1/33
CPC分类号： C12M27/00 , C12M29/14 , C12M45/02 , C12M47/06 , C12M47/08 , C12N1/066 , F04B9/113 , F04B31/00
摘要：【解決手段】長尺シリンダ1、2つの主連結スリーブ2、2つの高圧シリンダ均質化本体3、2つの付随連結スリーブ4、および2つの短尺シリンダ5を備えた同軸線ダブル高圧シリンダ均質一体化装置であって、2つの主連結スリーブ2、2つの高圧シリンダ均質化本体3、2つの付随連結スリーブ4、および2つの短尺シリンダ5はいずれも前記長尺シリンダ1の両端にそれぞれ対称に設けられ、かついずれも長尺シリンダ1と同軸心線に取り付けられている。【選択図】図1

15. 发明专利

JP2013255447A Cell isolation apparatus 审中-公开
标题翻译：细胞分离装置
公开(公告)号：JP2013255447A
公开(公告)日：2013-12-26
申请号：JP2012133090
申请日：2012-06-12
申请人： Nippon Koden Corp , 日本光電工業株式会社
发明人： SHIOYAMA TAKAHIRO , SUZUKI AKANE , KUBO HIROTSUGU
IPC分类号： C12M1/26 , C12M3/08
CPC分类号： B01L3/021 , C12M45/02 , C12M47/08
摘要： PROBLEM TO BE SOLVED: To provide a technique by which a certain result of cell isolation can be automatically obtained without performing a procedure that depends on the skill level.SOLUTION: In a cell isolation apparatus 100, tissue and fluid that are accommodated in a container 20 are agitated by a pipette 10, and cells are isolated from the tissue. A nozzle 40 to which the pipette 10 is attached is connected to a pump 42. A controller 80 controls an operation of the pump 42 to cause air to be ejected from the nozzle 40 or to be sucked into the nozzle, thereby causing the pipette 10 to perform agitation. A user inputs a condition of control through an operation unit 94. As the condition of the control, at least one of a rate of ejection of air from the nozzle 40, a rate of suction of air into the nozzle, an amount of suction of air, an amount of ejection of air, a duration time of ejection of air, a duration time of suction of air, an interval between election and suction, and ejection and suction numbers is variable.
摘要翻译：要解决的问题：提供一种可以在不执行取决于技能水平的程序的情况下自动获得细胞分离的某些结果的技术。解决方案：在细胞分离装置100中，容纳在容器中的组织和流体 20被移液管10搅动，细胞与组织分离。将吸移管10安装到其上的喷嘴40连接到泵42.控制器80控制泵42的操作，以使空气从喷嘴40喷射或被吸入喷嘴，从而使移液管10 进行激动。用户通过操作单元94输入控制条件。作为控制条件，从喷嘴40排出空气的速率，进入喷嘴的空气吸入速率，吸入量空气，空气喷射量，空气喷射持续时间，空气吸入持续时间，选择和抽吸之间的间隔以及喷射和吸入数量是可变的。

16. 发明专利

JP4592474B2 High pressure homogenization equipment, and high-pressure homogenization method 有权
公开(公告)号：JP4592474B2
公开(公告)日：2010-12-01
申请号：JP2005103245
申请日：2005-03-31
申请人：成雄安藤 , 正雄安藤 , 豊禄安藤
发明人：成雄安藤 , 正雄安藤 , 豊禄安藤
IPC分类号： B01F5/06 , B01F3/08 , C12M1/33 , C12N1/00
CPC分类号： B01F5/0663 , B01F3/12 , B01F15/0201 , B01F15/0237 , B01F15/0437 , B01F2215/0014 , B01F2215/0016 , B01F2215/0022 , B01F2215/0031 , B01F2215/0034 , B01F2215/0049 , B01F2215/0078 , B01F2215/0468 , C12M47/06 , C12M47/08 , D21B1/36

17. 发明专利

JP2007216103A Homogenizer 有权
标题翻译：均质
公开(公告)号：JP2007216103A
公开(公告)日：2007-08-30
申请号：JP2006037096
申请日：2006-02-14
申请人： Japan Organo Co Ltd , オルガノ株式会社
发明人： TANAKA JUICHI
IPC分类号： B02C1/00
CPC分类号： C12M47/08
摘要： PROBLEM TO BE SOLVED: To enable the sensing of a timing when a sample is crushed without fail, and the simplification of a complete examination, and to aim a reduction in production cost. SOLUTION: A paddle speed is stabilized by an output of a control signal adjusting a rotational speed of a motor 11 from a paddle speed control means 151. An output value (control output value) of the control signal from the paddle speed control means to the motor 11 decreases gradually in the rate of change when a crushing of samples advances and a homogenization advances. At the time when the sample is crushed without fail, the control output value falls in a value within the predetermined range. Therefore, by providing a control output value confirming means 16 capable of confirming that such a control output value falls in the value within the predetermined range, a finish of a crushing process of the sample can be known easily. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：为了能够感测到样品被破碎的时机，并且简化了完整的检查，并且旨在降低生产成本。解决方案：通过控制信号的输出稳定桨速，该控制信号从桨速控制装置151调节马达11的转速。来自桨速控制装置的控制信号的输出值（控制输出值）当样品的破碎进行并且均质化进行时，电动机11的平均值在变化率中逐渐降低。当样品被破碎时，控制输出值落在预定范围内的值。因此，通过提供能够确认这样的控制输出值落在规定范围内的值的控制输出值确认单元16，可以容易地知道样品的破碎处理的结束。版权所有（C）2007，JPO＆INPIT

18. 发明专利

JP2004049105A Cell disintegrator and method for disintegrating cell, therapeutic device and method for selectively isolating cell component 有权
公开(公告)号：JP2004049105A
公开(公告)日：2004-02-19
申请号：JP2002210823
申请日：2002-07-19
申请人： Mari Ikeda , Terutake Ueno , 上野照剛 , 池田真理
发明人： UENO TERUTAKE , IKEDA MARI
IPC分类号： C12M1/33 , C12N5/07 , C12N5/071 , C12N5/06
CPC分类号： C12M47/08 , C12M45/02
摘要： PROBLEM TO BE SOLVED: To provide a cell disintegrator and a cell-disintegrating method enabling a target cell in a sample to be disintegrable selectively and physically without imparting any heat, and to provide a method for selectively and efficiently isolating only a cell constituent such as DNA in the target cell.
SOLUTION: The cell disintegrator comprises particles specifically bindable to the target cell and movable on being subjected to an external stimulation and a target cell-disintegrating means functioning to subject the particles specifically bound to the target cell to an external stimulation to move them and disintegrate the target cell. The cell-disintegrating method comprises the steps of adding the particles to a sample containing the target cell to specifically bind them to the target cell and of subjecting the resultant particles to an external stimulation to move them and disintegrate the target cell. The method for selectively isolating cell component(s) comprises the step of centrifuging a sample treated by the cell-disintegrating method.
COPYRIGHT: (C)2004,JPO

19. 发明专利

JP5883812B2 破砕処理装置及び保冷庫有权
标题翻译：破碎加工设备和冷藏
公开(公告)号：JP5883812B2
公开(公告)日：2016-03-15
申请号：JP2013037906
申请日：2013-02-27
申请人：シスメックス株式会社
发明人：陳東克彦 , 大井義規 , 朝田祥一郎 , 尾日向大樹 , 正見圭一郎
IPC分类号： C12M1/33
CPC分类号： C12M45/02 , B01F13/1022 , B01F13/1041 , B01F15/065 , C12M47/08 , C12M47/20

20. 发明专利

JP4651987B2 Cell disruption device and a temperature control method 有权
公开(公告)号：JP4651987B2
公开(公告)日：2011-03-16
申请号：JP2004226605
申请日：2004-08-03
申请人：安井器械株式会社
发明人：修二安井
IPC分类号： C12M1/33 , B02C17/14 , B02C17/18
CPC分类号： C12M47/06 , C12M47/08 , C12M47/20

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