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    • 8. 发明公开
    • Amino group containing support matrices, their use and manufacture
    • 氨基葡萄柚Trägermatritzen,Verwendung und Herstellung davon
    • EP1433795A2
    • 2004-06-30
    • EP04008121.8
    • 2000-03-22
    • Amersham Biosciences AB
    • Fonnum, Geir
    • C08F212/04C08F8/00
    • B01J20/285B01J20/26B01J20/28042B01J2220/82C08F8/00C08F212/14C08F212/04
    • A method for the manufacture of a support matrix exhibiting amino groups I, possibly in acylated form, comprising the step of polymerising one or more monovinyl monomers (monomer I) with one or more di- tri- or polyvinyl monomers (monomer II). The method is characterized in that that the polymerisation is run in the presence of one or more amino-(C 0-10 )hydrocarbon vinyl aromatic monomers possibly in acylated form (monomer III.
      The preferred combination of monomers are ethyl vinyl benzene, divinyl benzene and amino styrene.
      A support matrix comprising a polyvinyl backbone [(-CH 2 CH 2 ) n ] and amino groups or groups derived from said amino groups while retaining the nitrogen of the amino group, each of which groups being attached to said backbone via a link structure containing an arylene group. The matrix is charactetized in that each of said groups is directly attached via said nitrogen to said arylene group.
      The use of the support matrix as defined in any one of claims 1-17, for solid phase synthesis, for instance of an oligo/poly-nucleotide or an oligo/polypeptide.
    • 制备显示氨基I的载体基质的方法,可能是酰化形式,包括使一种或多种单乙烯基单体(单体I)与一种或多种二 - 或聚乙烯基单体(单体II)聚合的步骤。 该方法的特征在于聚合在一种或多种可能具有酰化形式的氨基 - (C0-10)烃乙烯基芳族单体的存在下进行(单体III)。单体的优选组合是乙基乙烯基苯 ,二乙烯基苯和氨基苯乙烯。一种载体基质,其包含聚乙烯基主链Ä(-CH 2 CH 2)nÜ和氨基或衍生自所述氨基的基团,同时保留氨基的氮,其中每个基团连接到 所述骨架通过含有亚芳基的连接结构,所述基质的特征在于,每个所述基团通过所述氮直接连接到所述亚芳基。使用如权利要求1中任一项所述的载体基质 -17,用于固相合成,例如寡聚/多核苷酸或寡核苷酸/多肽。