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1. 发明公开

EP0580694A4 PRODUCTION OF PHOSPHOPEPTIDES FROM CASEIN 有权转让
标题翻译：从CASEIN生产磷酸盐
公开(公告)号：EP0580694A4
公开(公告)日：1995-12-27
申请号：EP92908863
申请日：1992-04-16
申请人： UNIV MELBOURNE , VICTORIAN DAIRY IND
发明人： REYNOLDS ERIC CHARLES
IPC分类号： C07K7/08 , A23J1/22 , A23J3/10 , A61K38/00 , C07K1/00 , C07K1/34 , C07K14/00 , C07K14/47 , C12P21/06 , C07K3/02 , C07K7/10 , C07K15/24
CPC分类号： C07K14/4732 , A61K38/00
摘要： A method for the preparation of selected phosphopeptides having anticariogenic and other activities, comprising the steps of completely digesting a soluble monovalent cation salt of casein in solution with a proteolytic enzyme, adding a mineral acid to the solution to adjust the pH to about 4.7, removing any precipitate produced, adding CaCl2 to a level of about 1.0 % w/v to cause aggregation of at least the selected phosphopeptides in said digested solution, separating the aggregated phosphopeptides from the solution through a filter having a molecular weight exclusion limit lying substantially within the range 10,000 to 20,000 while passing the bulk of the remaining phosphopeptides and solution, diafiltering the separated phosphopeptides with water through a filter and concentrating and drying the retentate.

2. 发明授权

EP0464101B1 IMPROVEMENTS RELATING TO THE RADIO-LABELLING OF PROTEINS 失效转让
标题翻译：単蛋白质。
公开(公告)号：EP0464101B1
公开(公告)日：1994-11-23
申请号：EP90905248.2
申请日：1990-03-23
申请人： BRITISH TECHNOLOGY GROUP LIMITED
发明人： FOXWELL, Brian, Maurice, John , PARKER, Peter , CREIGHTON, Andrew, Malcolm
IPC分类号： C07H19/10 , C07H19/20 , A61K37/16 , C12P21/00 , C07K15/24
CPC分类号： A61K51/0491 , A61K51/1093 , A61K2121/00 , C07H19/10 , C07H19/20 , C12P21/00
摘要： Nucleoside thiotriphosphates carrying 32P in the gamma-thiophosphate group, prepared by reaction between a nucleoside diphosphate and a 32P labelled thiophosphate salt, are used as thiophosphorylating agents for antibodies that bind to tumour-associated antigens. The labelled thiotriphosphates can be used to introduce a therapeutically useful 32P atom into an antibody that has been modified by the incorporation into its structure of a peptide region capable of acting as a substrate for a phosphokinase. The resulting labelled antibody can then be used for injection in anti-tumour therapy and has clinical advantages over the corresponding phosphorylated analogue.

3. 发明授权

EP0145373B1 Purification of cancer-associated protein and preparation of antibody thereto 有权
标题翻译：癌症相关蛋白的纯化和抗体的制备
公开(公告)号：EP0145373B1
公开(公告)日：1992-03-25
申请号：EP84308081.3
申请日：1984-11-21
申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
发明人： Webb, Thomas E. , Schumm, Dorothy E. , Hanausek-Walaszek, Margaret
IPC分类号： G01N33/574 , G01N33/577 , C07K3/02 , C12N15/00 , C07K15/06 , C07K15/24 , C07K3/28
CPC分类号： C07K16/30

4. 发明公开

EP0637970A4 POLYAMINO ACID SUPERABSORBENTS. 失效
标题翻译：聚高吸水性树脂。
公开(公告)号：EP0637970A4
公开(公告)日：1996-01-10
申请号：EP93909195
申请日：1993-04-06
申请人： UNIV SOUTH ALABAMA
发明人： DONACHY JULIE , SIKES C STEVEN
IPC分类号： A61L15/00 , A61F13/53 , A61L15/60 , B01J20/22 , C07K2/00 , C07K14/00 , C08G69/10 , C08G73/10 , C09K3/00 , A61L15/32 , C07K15/12 , C07K15/24 , B01J20/26 , C07K1/00 , C08F283/00 , C08L77/04
CPC分类号： C08G73/1092 , A61L15/60 , C07K14/001 , C08G69/10 , C08L77/00
摘要： Substantially water-insoluble, crosslinked polypeptides containing 15 to 85 mole % of amino acid residues such as glutamic acid, aspartic acid, phosphoserine, phosphohomoserine, phosphotyrosine, phosphothreonine, phosphoasparagine, or phosphoglutamine, and 15 to 85 mole % of amino acid residues such as lysine, arginine, asparagine, glutamine, serine or tyrosine, in which the degree of crosslinking is sufficient to result in a substantially water-insoluble polypeptide with the ability to absorb a 1 wt.% aqueous NaCl solution in an amount of at least 20 times the weight of the polypeptide, are useful as superabsorbents in devices such as diapers, etc. Mild alkaline hydrolysis of the crosslinked polypeptides increases their superabsorbency by two to three fold.

5. 发明公开

EP0145373A3 Purification of cancer-associated protein and preparation of antibody thereto 有权
标题翻译：癌症相关蛋白的纯化和抗体的制备
公开(公告)号：EP0145373A3
公开(公告)日：1985-07-17
申请号：EP84308081
申请日：1984-11-21
申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
发明人： Webb, Thomas E. , Schumm, Dorothy E. , Hanausek-Walaszek, Margaret
IPC分类号： G01N33/574 , G01N33/577 , C07K03/02 , C12N15/00 , C07K15/06 , C07K15/24 , C07K03/28
CPC分类号： C07K16/30
摘要： It has previously been shown that the serum from patients suffering from a wide range of cancers contains a cancer marker protein having the ability to release RNA from cell nuclei. This cancer marker protein is purified by taking the protein fraction precipitating between 30% and 50% saturated aqueous ammonium sulfate solution, dialyzing a solution of the protein fraction against TMK buffer, chromatographing the dialyzed protein on a molecular sieve and isolating the fraction having a molecular weight of about 60,000; and removing albumin. Injection of the purified protein into rabbits, preparation of serum from blood of the rabbits and absorption of the sera with normal plasma fraction produces an antibody specific to the cancer marker protein and therefore useful in a radioimmunoassay or ELISA test for a wide variety of cancers. Monoclonal antibody preparations to the protein may be prepared by injecting the purified protein into mice, harvesting the spleen and lymph node cells, fusing these cells with mouse myeloma cells and using the resultant hybridoma cells to produce the monoclonal antibody.

6. 发明公开

EP0145373A2 Purification of cancer-associated protein and preparation of antibody thereto 有权
标题翻译： Reinigung eines krebsassoziierten蛋白和Herstellung einesAntikörpersdazu。
公开(公告)号：EP0145373A2
公开(公告)日：1985-06-19
申请号：EP84308081.3
申请日：1984-11-21
申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
发明人： Webb, Thomas E. , Schumm, Dorothy E. , Hanausek-Walaszek, Margaret
IPC分类号： G01N33/574 , G01N33/577 , C07K3/02 , C12N15/00 , C07K15/06 , C07K15/24 , C07K3/28
CPC分类号： C07K16/30
摘要： It has previously been shown that the serum from patients suffering from a wide range of cancers contains a cancer marker protein having the ability to release RNA from cell nuclei. This cancer marker protein is purified by taking the protein fraction precipitating between 30% and 50% saturated aqueous ammonium sulfate solution, dialyzing a solution of the protein fraction against TMK buffer, chromatographing the dialyzed protein on a molecular sieve and isolating the fraction having a molecular weight of about 60,000; and removing albumin. Injection of the purified protein into rabbits, preparation of serum from blood of the rabbits and absorption of the sera with normal plasma fraction produces an antibody specific to the cancer marker protein and therefore useful in a radioimmunoassay or ELISA test for a wide variety of cancers. Monoclonal antibody preparations to the protein may be prepared by injecting the purified protein into mice, harvesting the spleen and lymph node cells, fusing these cells with mouse myeloma cells and using the resultant hybridoma cells to produce the monoclonal antibody.
摘要翻译：以前已经显示来自患有广泛癌症的患者的血清含有具有从细胞核释放RNA的能力的癌症标志物蛋白质。通过将蛋白质级分沉淀在30％至50％饱和的硫酸铵水溶液中，透析蛋白质级分与TMK缓冲液的溶液，将分析筛上的透析蛋白色谱分离，并分离出分子重量约6万; 并去除白蛋白。将纯化的蛋白质注射到兔中，从兔血液制备血清和用正常血浆级分吸收血清产生对癌症标记蛋白特异性的抗体，因此可用于多种癌症的放射免疫测定或ELISA测试。可以通过将纯化的蛋白质注入小鼠，收获脾脏和淋巴结细胞，将这些细胞与小鼠骨髓瘤细胞融合并使用所得的杂交瘤细胞产生单克隆抗体来制备该蛋白质的单克隆抗体制剂。

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