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1. 发明授权

EP2986359B1 DIP-STICK WESTERN BLOT 有权转让
公开(公告)号：EP2986359B1
公开(公告)日：2024-08-14
申请号：EP15761295.3
申请日：2015-03-11
IPC分类号： G01N27/447 , B01D57/02 , C02F1/40 , G01N30/90
CPC分类号： G01N30/90 , G01N27/44739 , G01N33/54386

2. 发明授权

EP3662276B1 ELECTROPHORETIC SEPARATION OF BIOMOLECULES 有权转让
公开(公告)号：EP3662276B1
公开(公告)日：2024-07-10
申请号：EP17746479.9
申请日：2017-08-01
IPC分类号： G01N27/447 , B43L17/00 , B43L3/00
CPC分类号： G01N27/44739

3. 发明授权

EP1872117B1 EXPANDING CAM LOCK FOR SEALING SLAB GELS IN AN ELECTROPHORESIS APPARATUS 有权转让
标题翻译： EXTENDABLE CAM STOPPER用于密封凝胶板电泳
公开(公告)号：EP1872117B1
公开(公告)日：2017-03-29
申请号：EP06750059.5
申请日：2006-04-14
申请人： Life Technologies Corporation
发明人： JACKSON, Tom, R.
IPC分类号： G01N27/447
CPC分类号： G01N27/44739 , B01L3/502 , B01L3/563 , B01L3/565 , B01L9/52 , B01L2200/025 , B01L2200/026 , B01L2200/04 , B01L2200/0689 , B01L2300/041 , B01L2300/06 , B01L2300/0848 , B01L2300/0851 , B01L2400/0421 , B33Y80/00 , C07H1/06 , C07K1/26 , F16B2/18 , G01N27/44704 , Y10T29/49117 , Y10T403/7009
摘要： An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. In one example embodiment, the expanding cam lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. The base plate and the follower plate are slidably coupled together and are designed for insertion between the first buffer core assembly and the second buffer core assembly, buffer dam or buffer displacement dam in the electrophoresis container. A cam is positioned between and moveably coupled with the base plate and the follower plate. The cam is movable from a first position to a second position to urge the first and second surfaces to secure the gel cassette to the first and second buffer core assemblies. Also provided herein is a buffer displacement dam. Also provided herein are kits and assemblies which incorporate the expanding cam and buffer displacement dam described herein.

4. 发明公开

EP3128320A1 SEPARATION MEDIUM CASSETTE FOR SAMPLE SEPARATION ADSORPTION AND ANALYSIS DEVICE FOR SAMPLE SEPARATION ADSORPTION 审中-公开
标题翻译：新加坡民主共和国人民共和国民族解放运动
公开(公告)号：EP3128320A1
公开(公告)日：2017-02-08
申请号：EP15772882.5
申请日：2015-03-13
申请人： Sharp Kabushiki Kaisha
发明人： TOMINAGA, Taiga , UNUMA, Yutaka , OHKI, Hiroshi , GOTO, Shinichi
IPC分类号： G01N27/447
CPC分类号： G01N27/44743 , G01N27/44739 , G01N27/44756
摘要： Provided is a feature for performing more accurate analysis in a sample separation adsorption method. A gel cassette (22) is provided with a separation medium (19) for separating out a sample by electrophoresis and discharging the sample, a first insulating portion (13a) and a second insulating portion (13b) sandwiching the separation medium, and warping prevention portions (20) that are inserted between the first insulating portion (13a) and the second insulating portion (13b), and that mitigate warping of the first insulating portion (13a) and the second insulating portion (13b).
摘要翻译：提供了在样品分离吸附方法中进行更准确的分析的特征。凝胶盒（22）设置有用于通过电泳和样品分离出样品的分离介质（19），夹着分离介质的第一绝缘部分（13a）和第二绝缘部分（13b），并且防翘曲插入在第一绝缘部分（13a）和第二绝缘部分（13b）之间的部分（20），并且减轻第一绝缘部分（13a）和第二绝缘部分（13b）的翘曲。

5. 发明公开

EP2877483A4 TARGETED DELIVERY OF REAGENTS THROUGH PATTERNED TRANSFER SHEETS 有权
标题翻译： BY转移EYED薄膜试剂靶向递送
公开(公告)号：EP2877483A4
公开(公告)日：2016-07-06
申请号：EP13823401
申请日：2013-07-25
申请人： BIO RAD LABORATORIES
发明人： MCKEE CLAYTON T , STRONG WILLIAM
IPC分类号： C07K1/04 , C07K1/14 , C12M1/00 , G01N27/447
CPC分类号： G01N27/44739 , G01N27/44747 , G01N33/54306 , G01N33/54393
摘要： Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

6. 发明公开

EP2906935A1 SIDE-ELUTING MOLECULAR FRACTIONATOR 有权
标题翻译：侧面分子分裂剂
公开(公告)号：EP2906935A1
公开(公告)日：2015-08-19
申请号：EP13780497.7
申请日：2013-10-10
申请人： Sage Science, Inc.
发明人： SABIN, Douglas, Grosvenor , GOMES, Joshua , BARBERA, Todd, J. , SIDOTI, Charles , SINGH, Simranjit , BOLES, Christian, T.
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44739
摘要： The disclosure provides cassettes, electrophoresis systems, methods for making the device, and methods of fractionating a sample using the cassettes and electrophoresis systems described herein.
摘要翻译：本公开提供了一种电泳盒（502），其包括用于分离样品的宏观流体分离通道（525），用于从分离通道提取分离的级分的洗脱通道（522,523）以及用于收集提取的分离的级分的洗脱模块（524）。制造该装置的方法，以及使用本文所述的盒和电泳系统分馏样品的方法。

7. 发明公开

EP2812700A1 SIGNATURES AND DETERMINANTS FOR DIAGNOSING INFECTIONS AND METHODS OF USE THEREOF 有权
标题翻译： SIGNATUREN UND DETERMINENTEN ZUR DIAGNOSE VON INFEKTIONEN UND VERWENDUNGSVERFAHRENDAFÜR
公开(公告)号：EP2812700A1
公开(公告)日：2014-12-17
申请号：EP13703112.6
申请日：2013-02-08
申请人： Memed Diagnostics Ltd.
发明人： OVED, Kfir , EDEN, Eran , IFERGAN, Ilan
IPC分类号： G01N33/569
CPC分类号： G01N33/56983 , G01N27/447 , G01N27/44726 , G01N27/44739 , G01N33/56911 , G01N33/6863 , G01N33/6866 , G01N2333/70575 , G01N2800/52
摘要： Antibiotics (Abx) are the world's most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment.
摘要翻译：抗生素（Abx）是世界上滥用最多的药物。当在无效的非细菌感染（例如病毒感染）的情况下施用药物时，发生抗生素滥用。总的来说，据估计，世界范围内有40-70％的Abx课程是错误的。 Abx过度处方的财务和健康后果包括药物的直接成本以及其副作用的间接成本，估计每年超过150亿美元。此外，过度处方直接导致Abx抗性菌株的出现，这被认为是今天对公共卫生的主要威胁之一。这产生了对可靠诊断的立即需求，以帮助医生进行正确的Abx处方，特别是在大多数Abx处方的护理点（POC）方面。因此，本发明的一些方面提供使用生物标志物快速检测感染源并施用适当治疗的方法。

8. 发明公开

EP2509704A4 ELECTROTRANSFER CASSETTE WITH INTEGRATED ELECTRICAL CONTACTS AND LOCKING MECHANISM 有权
标题翻译：具有集成开关触点和锁定机构电气传动CASSETTE
公开(公告)号：EP2509704A4
公开(公告)日：2014-09-03
申请号：EP10836715
申请日：2010-12-09
申请人： BIO RAD LABORATORIES
发明人： LATHAM MATTHEW
IPC分类号： B01D57/02 , A44B17/00 , G01N27/447 , G01N33/559
CPC分类号： B01D57/02 , G01N27/44739 , Y10T292/0837

9. 发明公开

EP2398819A4 ELECTROBLOTTING CASSETTE WITH MANUALLY RELEASABLE ELECTRODES OF ADJUSTABLE SPACING 有权
标题翻译：电印迹托盘手动装卸领导一个可调整的距离
公开(公告)号：EP2398819A4
公开(公告)日：2013-10-30
申请号：EP10744427
申请日：2010-02-22
申请人： BIO RAD LABORATORIES
发明人： LATHAM MATTHEW
IPC分类号： G01N27/447
CPC分类号： B01D57/02 , G01N27/44739

10. 发明公开

EP2572189A1 WESTERN BLOT ANALYTICAL TECHNIQUE 有权转让
标题翻译：免疫印迹，ANALYSEVERFAHREN
公开(公告)号：EP2572189A1
公开(公告)日：2013-03-27
申请号：EP11721299.3
申请日：2011-05-20
申请人： Lab901 Limited
发明人： MACNAMARA, Kenneth G , POLWART, Stuart
IPC分类号： G01N27/447 , G01N33/53 , G01N33/68 , G01N1/04
CPC分类号： G01N27/44726 , G01N27/44739 , G01N33/6803
摘要： In accordance with an embodiment of the present invention, there is provided a method of performing the western blot analytical technique, wherein the method comprises carrying out the following steps in the following order: a), pre-staining the proteins within a sample; b). separating the proteins using gel electrophoresis; c). analysing the separated proteins to determine the total protein load and/or at least one housekeeping protein load; d). transferring the proteins onto at least one membrane; e). probing the separated proteins to detect a target protein; and f). analysing the probed proteins to determine the target protein's molecular weight and/or load.
摘要翻译：根据本发明的实施方案，提供了一种进行蛋白质印迹分析技术的方法，其中所述方法包括按以下顺序进行以下步骤：a）预先染色样品内的蛋白质; B）。使用凝胶电泳分离蛋白质; C）。分析分离的蛋白质以确定总蛋白质负荷和/或至少一个内部蛋白质负载; D）。将蛋白质转移到至少一个膜上; E）。探测分离的蛋白质以检测靶蛋白; 和f）。分析探测蛋白质以确定靶蛋白的分子量和/或负荷。

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