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1. 发明授权

EP2236613B1 Biochemical synthesis of 1,4-butanediamine 有权
标题翻译： 1,4-丁二胺的生物化学合成
公开(公告)号：EP2236613B1
公开(公告)日：2015-05-27
申请号：EP10164437.5
申请日：2005-07-11
申请人： DSM IP Assets B.V.
发明人： Eppelmann, Katrin , Nossin, Petrus Martinus Matheus , Kremer, Susanne Maria , Wubbolts, Marcel Gerhardus
IPC分类号： C12N1/21 , C12N9/10 , C12N9/88 , C12P13/00
CPC分类号： C12N9/1029 , C12N9/78 , C12N9/80 , C12N9/88 , C12P13/001 , C12Y305/01053 , C12Y305/03011 , C12Y305/03012 , C12Y401/01017 , Y02P20/52

2. 发明授权

EP1781799B1 BIOCHEMICAL SYNTHESIS OF 1,4-BUTANEDIAMINE 有权
标题翻译： 1,4-丁二醇生化合成
公开(公告)号：EP1781799B1
公开(公告)日：2011-04-06
申请号：EP05774363.5
申请日：2005-07-11
申请人： DSM IP Assets B.V.
发明人： EPPELMANN, Katrin , NOSSIN, Petrus, Martinus, Matheus , KREMER, Susanne, Maria , WUBBOLTS, Marcel, Gerhardus
IPC分类号： C12P13/00 , C12N9/88 , C12N9/10
CPC分类号： C12N9/1029 , C12N9/78 , C12N9/80 , C12N9/88 , C12P13/001 , C12Y305/01053 , C12Y305/03011 , C12Y305/03012 , C12Y401/01017 , Y02P20/52
摘要： The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene speA and an agmatinase encoding gene speB; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB. The invention also relates to vectors, plasmids and hosts carrying, at an increased level of activity, one or more of the enzyme activities as mentioned.

3. 发明授权

EP3141598B1 MICROORGANISMS FOR PRODUCING PUTRESCINE OR ORNITHINE AND PROCESS FOR PRODUCING PUTRESCINE OR ORNITHINE USING THEM 有权转让
公开(公告)号：EP3141598B1
公开(公告)日：2018-10-31
申请号：EP16180193.1
申请日：2016-07-19
申请人： CJ Cheiljedang Corporation
发明人： PARK, Su Jin , YANG, Young Lyeol , UM, Hye Won , LI, Hong Xian , LEE, Kyoung Min , LEE, Baek Seok , LEE, Hyo Hyoung , JUNG, Hee Kyoung
IPC分类号： C12N1/21 , C12N9/80 , C12N9/10 , C12N15/52 , C12P13/00
CPC分类号： C12N9/1029 , C12N9/1217 , C12N9/80 , C12N9/88 , C12N9/93 , C12N15/52 , C12P13/001 , C12P13/10 , C12Y203/01001 , C12Y203/01008 , C12Y207/02001 , C12Y305/01016 , C12Y401/01017 , C12Y602/01001
摘要： Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same.

4. 发明公开

EP3268468A1 L-ORNITHINE PRODUCTION IN EUKARYOTIC CELLS 审中-公开
标题翻译： L-ORNITHINE在真核细胞中的生产
公开(公告)号：EP3268468A1
公开(公告)日：2018-01-17
申请号：EP16714581.2
申请日：2016-03-10
申请人： Biopetrolia AB
发明人： QIN, Jiufu , KRIVORUCHKO, Anastasia , DAVID, Florian , JIANG, Bo , NIELSEN, Jens
IPC分类号： C12N9/10 , C12N15/52 , C12P13/00 , C12P13/10
CPC分类号： C12P13/10 , C12N9/0008 , C12N9/1018 , C12N9/1096 , C12N9/1217 , C12N9/78 , C12N9/88 , C12N15/52 , C12P13/001 , C12Y102/01038 , C12Y201/03003 , C12Y206/01011 , C12Y207/02008 , C12Y305/03001 , C12Y401/01017 , C12Y403/02001
摘要： The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

5. 发明公开

EP3023493A2 NOVEL MUTANT ORNITHINE DECARBOXYLASE PROTEIN AND USE THEREOF 有权
标题翻译：新型它们的用途突变ORNITHINDECARBOXYLASEPROTEIN AND
公开(公告)号：EP3023493A2
公开(公告)日：2016-05-25
申请号：EP14826660.4
申请日：2014-07-17
申请人： Cj Cheiljedang Corporation , Korea Advanced Institute Of Science And Technology
发明人： CHOI, Hyang , JUNG, Hee Kyoung , KIM, Hak Sung , KYEONG, Hyun Ho , CHOI, Jung Min , LEE, Joong Jae , SEO, Hyo Deok
IPC分类号： C12N9/88 , C12N15/60 , C12P13/04
CPC分类号： C12P13/001 , C12N9/88 , C12Y401/01017
摘要： Provided are a novel modified ornithine decarboxylase protein having improved putrescine productivity and a use thereof.

6. 发明公开

EP4412709A1 NEMATODE VACCINE 审中-实审
公开(公告)号：EP4412709A1
公开(公告)日：2024-08-14
申请号：EP22878045.8
申请日：2022-10-03
申请人： AgResearch Limited
发明人： UMAIR, Saleh , KNIGHT, Jacqueline Sarah
IPC分类号： A61P33/10 , A61K39/00 , C07K14/435 , C12N15/70 , C12N9/12 , C12N9/88
CPC分类号： A61P33/10 , C12N9/88 , C12N15/70 , C07K14/4354 , C12N9/1223 , C12Y207/03003 , C12Y401/01017 , C12Y402/01011 , A61K39/0003 , A61K2039/55220130101 , A61K2039/5557720130101 , A61K2039/5550520130101 , A61K2039/57520130101

7. 发明公开

EP2650357A2 MICROORGANISMS FOR PRODUCING PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING SAME 有权
标题翻译：用于微生物生产腐胺和腐胺PROCESS FOR这样就产生
公开(公告)号：EP2650357A2
公开(公告)日：2013-10-16
申请号：EP11846494.0
申请日：2011-12-08
申请人： CJ CheilJedang Corporation
发明人： CHOI, Hyang , LEE, Kyoung Min , KANG, Min Sun , JHON, Sung Hoo , UM, Hye Won , CHOI, Su Jin , LEE, Han Won , SHIN, Soo An
IPC分类号： C12N1/21 , C12N15/74 , C12N15/69 , C12P13/00
CPC分类号： C12P13/001 , C07K14/34 , C12N9/0008 , C12N9/1018 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/88 , C12R1/15 , C12Y102/01038 , C12Y201/03003 , C12Y203/01035 , C12Y206/01013 , C12Y207/02008 , C12Y401/01017
摘要： The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.
摘要翻译：本发明涉及一种腐胺生产微生物和用于使用相同的生产腐胺的方法。更具体地，本发明涉及赋予产生腐胺，其是通过从鸟氨酸阻断生物合成途径生成的所有成精氨酸的能力的微生物，增加谷氨酸盐的胞内水平，增强从谷氨酸鸟氨酸的生物合成途径，并引入胞外鸟氨酸脱羧酶; 以及用于通过使用该微生物生产腐胺的方法。

8. 发明公开

EP2236613A1 Biochemical synthesis of 1,4-butanediamine 有权
标题翻译： Biochemische Synthese von 1,4-Butandiamin
公开(公告)号：EP2236613A1
公开(公告)日：2010-10-06
申请号：EP10164437.5
申请日：2005-07-11
申请人： DSM IP Assets B.V.
发明人： Eppelmann, Katrin , Nossin, Petrus Martinus Matheus , Kremer, Susanne Maria , Wubbolts, Marcel Gerhardus
IPC分类号： C12P13/00 , C12N9/88 , C12N9/10
CPC分类号： C12N9/1029 , C12N9/78 , C12N9/80 , C12N9/88 , C12P13/001 , C12Y305/01053 , C12Y305/03011 , C12Y305/03012 , C12Y401/01017 , Y02P20/52
摘要： The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1 ,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth.
In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene spa4 and an agmatinase encoding gene speB ; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB.
The invention also relates to vectors, plasmids and hosts carrying, at an increased level of activity, one or more of the enzyme activities as mentioned.
摘要翻译：本发明涉及一种与天然水平的鸟氨酸脱羧酶活性相比具有升高的鸟氨酸脱羧酶活性水平（增加的ODC活性）的微生物中1,4-丁二胺的生化合成方法，其中增加的ODC活性为通过过度表达具有增加的翻译和/或转录效率的鸟氨酸脱羧酶编码基因获得的，并且其中在微生物中产生的1,4-丁二胺被排泄到发酵液中，并从发酵液中回收。在优选的实施方案中，还可以通过（i）精氨酸脱羧酶编码基因spa4和编码基因speB的促炎素酶的过表达获得增加的酶活性; 或（ii）编码基因speA的精氨酸脱羧酶和编码基因aguA的丁酰氨基水解酶，以及编码氨基酸氨基酸酰基水解酶的基因aguB，以及任选地还有编码腺病毒基因agB的氨基丁酸酶。本发明还涉及载体，质粒和宿主，其以增加的活性水平携带如上所述的一种或多种酶活性。

9. 发明公开

EP1781799A1 BIOCHEMICAL SYNTHESIS OF 1,4-BUTANEDIAMINE 有权
标题翻译： 1,4-丁二醇生化合成
公开(公告)号：EP1781799A1
公开(公告)日：2007-05-09
申请号：EP05774363.5
申请日：2005-07-11
申请人： DSM IP Assets B.V.
发明人： EPPELMANN, Katrin , NOSSIN, Petrus, Martinus, Matheus , KREMER, Susanne, Maria , WUBBOLTS, Marcel, Gerhardus
IPC分类号： C12P13/00 , C12N9/88 , C12N9/10
CPC分类号： C12N9/1029 , C12N9/78 , C12N9/80 , C12N9/88 , C12P13/001 , C12Y305/01053 , C12Y305/03011 , C12Y305/03012 , C12Y401/01017 , Y02P20/52
摘要： The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene speA and an agmatinase encoding gene speB; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB. The invention also relates to vectors, plasmids and hosts carrying, at an increased level of activity, one or more of the enzyme activities as mentioned.

10. 发明公开

EP1559792A2 Gene-related RNAi transfection method 审中-公开
标题翻译： Verfahren zur Transfektioin Gen-bezogener RNAi
公开(公告)号：EP1559792A2
公开(公告)日：2005-08-03
申请号：EP04019589.3
申请日：2004-08-18
申请人： EPPENDORF AG
发明人： Remacle, José , Bülow, Sven
IPC分类号： C12Q1/68 , C12N15/11 , B01L3/00
CPC分类号： C12Y401/01017 , C12N15/111 , C12N15/1135 , C12N15/1137 , C12N2310/14 , C12N2310/53 , C12N2320/10 , C12Q1/68 , C12Q2521/301
摘要： The present invention relates to a novel method for determining the biological effect of a siRNA species in a cell. In particular, the present invention provides a method, wherein a support is provided, containing on predetermined locations thereof nucleic acids, representing or giving rise to a siRNA species. The support is covered by cells, into which the siRNA are taken up and exert their effect. Then the biological effect of the siRNA in the cell may be determined.
摘要翻译：本发明涉及一种确定细胞中siRNA物种生物学作用的新方法。特别地，本发明提供了一种方法，其中提供了在其预定位置上含有代表或产生siRNA物种的核酸的载体。载体被细胞覆盖，siRNA被摄取并发挥作用。然后可以测定siRNA在细胞中的生物学效应。

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