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1. 发明公开

EP2855672A1 METHOD FOR PRODUCING HYPERACTIVE TRANSPOSASE MUTANTS 有权
标题翻译：生产超活性转运蛋白突变体的方法
公开(公告)号：EP2855672A1
公开(公告)日：2015-04-08
申请号：EP13819663.9
申请日：2013-05-30
申请人： Savilahti, Harri
发明人： RASILA, Tiina
IPC分类号： C12N9/22
CPC分类号： C12N9/1241 , C12N9/22 , C12Q1/68 , C12Y301/22 , C12Y605/01
摘要： The present invention provides a method for producing a hyperactive MuA transposase variant comprising at least one single-amino-acid change, the method comprising the steps of modifying the nucleic acid encoding wild type MuA transposase in at least one of the positions 59, 97, 160, 179, 233, 254, 258, 302, 335, 340, 345, 374, 447, 464, 478, 482, 483, 487, 495, 507, 539, 594 or 617 so that the modified nucleic acid encodes a MuA transposase variant comprising at least one single-amino-acid change in its amino acid sequence, wherein said single-amino-acid change results in higher enzyme activity of the variant when compared to the wild type MuA transposase. The present invention also provides hyperactive MuA transposases and kits comprising the same.
摘要翻译：本发明提供一种用于生产多动MUA转变体包含至少一个单氨基酸改变的方法，所述方法包括修改所述的位置59，97中的至少一个编码野生型MUA转座的核酸的步骤， 160，179，233，254，258，302，335，340，345，374，447，464，478，482，483，487，495，507，539，594或617，使得经修饰的核酸编码MUA 其在所述氨基酸序列中包含至少一个单氨基酸改变，其中与野生型MuA转座酶相比，所述单氨基酸改变导致所述变体的更高的酶活性。本发明还提供了过度活化的MuA转座酶和包含其的试剂盒。

2. 发明授权

EP2855672B1 METHOD FOR PRODUCING HYPERACTIVE TRANSPOSASE MUTANTS 有权
公开(公告)号：EP2855672B1
公开(公告)日：2018-08-01
申请号：EP13819663.9
申请日：2013-05-30
申请人： Savilahti, Harri
发明人： RASILA, Tiina
IPC分类号： C12N9/22
CPC分类号： C12N9/1241 , C12N9/22 , C12Q1/68 , C12Y301/22 , C12Y605/01
摘要： The present invention provides a method for producing a hyperactive MuA transposase variant comprising at least one single-amino-acid change, the method comprising the steps of modifying the nucleic acid encoding wild type MuA transposase in at least one of the positions 59, 97, 160, 179, 233, 254, 258, 302, 335, 340, 345, 374, 447, 464, 478, 482, 483, 487, 495, 507, 539, 594 or 617 so that the modified nucleic acid encodes a MuA transposase variant comprising at least one single-amino-acid change in its amino acid sequence, wherein said single-amino-acid change results in higher enzyme activity of the variant when compared to the wild type MuA transposase. The present invention also provides hyperactive MuA transposases and kits comprising the same.

3. 发明公开

EP2327786A2 Site-specific serine recombinases and methods of their use 有权
标题翻译：斯特林佩斯
公开(公告)号：EP2327786A2
公开(公告)日：2011-06-01
申请号：EP10181444.0
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： Padidam, Malla
IPC分类号： C12N15/85 , C07H21/04
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22
摘要： The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site ( attP ) or a bacterial genomic recombination attachment site ( attB ), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is attB, the second recombination attachment site is attP and when the first recombination attachment site is attP, the second recombination attachment site is attB. The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.
摘要翻译：本发明提供了一种在真核细胞中获得位点特异性重组的方法，所述方法包括提供包含第一重组附着位点和第二重组附着位点的真核细胞; 使第一和第二重组附着位点与原核重组酶多肽接触，导致重组附着位点之间的重组，其中重组酶多肽可以介导第一和第二重组附着位点之间的重组，第一重组附着位点是噬菌体基因组重组附着物位点（attP）或细菌基因组重组附着位点（attB），第二重组位点是attB或attP，重组酶选自单核细胞增生利斯特氏菌噬菌体重组酶，化脓性链球菌噬菌体重组酶，枯草芽孢杆菌噬菌体重组酶，结核分枝杆菌噬菌体重组酶和耻垢分枝杆菌噬菌体重组酶，条件是当第一重组附着位点为attB时，第二重组附着位点为attP，当第一重组附着位点为attP时，第二重组附着物 ent站点是attB。本发明还描述了用于产生转基因细胞，组织，植物和动物的组合物，载体及其使用方法。本发明的组合物，载体和方法也可用于基因治疗应用。

4. 发明公开

EP4375373A2 CAS VARIANTS FOR GENE EDITING 审中-实审
公开(公告)号：EP4375373A2
公开(公告)日：2024-05-29
申请号：EP24155880.8
申请日：2014-12-12
申请人： President And Fellows Of Harvard College
发明人： LIU, David, R. , KOMOR, Alexis, Christine
IPC分类号： C12N15/10
CPC分类号： C12N15/102 , C12Y304/22062 , C12Y305/04001 , C12N9/6472 , C07K2319/8020130101 , A61K38/465 , A61K38/50 , C12Y305/04004 , C12Y305/04005 , C12N9/78 , C12N15/01 , C12Y305/04 , C12Y301/00 , C12N9/22 , C12Y301/22 , A61P11/00 , A61P13/02 , A61P13/12 , A61P17/00 , A61P19/00 , A61P21/00 , A61P25/00 , A61P25/28 , A61P3/00 , A61P35/00 , Y02A50/30
摘要： Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.

5. 发明授权

EP2327786B1 Site-specific serine recombinases and methods of their use 有权
标题翻译：具体站点Serinrekombinasen以及它们的使用方法
公开(公告)号：EP2327786B1
公开(公告)日：2016-04-27
申请号：EP10181444.0
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： Padidam, Malla
IPC分类号： C12N9/00 , C12N15/90
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22

6. 发明公开

EP2327786A3 Site-specific serine recombinases and methods of their use 有权
标题翻译：具体站点Serinrekombinasen以及它们的使用方法
公开(公告)号：EP2327786A3
公开(公告)日：2012-03-21
申请号：EP10181444.0
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： Padidam, Malla
IPC分类号： C12N15/85 , C07H21/04 , C12N9/00
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22
摘要： The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site ( attP ) or a bacterial genomic recombination attachment site ( attB ), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is attB, the second recombination attachment site is attP and when the first recombination attachment site is attP, the second recombination attachment site is attB. The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.

7. 发明公开

EP3112457A1 SITE-SPECIFIC SERINE RECOMBINASES AND METHODS OF THEIR USE 无效
标题翻译：位点特异性丝氨酸重组酶及其使用方法
公开(公告)号：EP3112457A1
公开(公告)日：2017-01-04
申请号：EP16167169.8
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： PADIDAM, Malla
IPC分类号： C12N9/00 , C12N15/90
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22
摘要： The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site ( attP ) or a bacterial genomic recombination attachment site ( attB ), the second recombination site is att B or att P , and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is att B, the second recombination attachment site is att P and when the first recombination attachment site is att P , the second recombination attachment site is att B . The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.
摘要翻译：本发明提供了用于在真核细胞中获得位点特异性重组的方法，所述方法包括提供包含第一重组连接位点和第二重组连接位点的真核细胞; 使第一和第二重组连接位点与原核重组酶连接酶多肽接触，导致重组连接位点之间的重组，其中重组酶连接位点介导第一和第二重组连接位点之间的重组，第一重组连接位点是噬菌体基因组重组连接位点（attP）或细菌基因组重组连接位点（attB），第二重组位点是attB或attP，重组酶选自单核细胞增生利斯特氏菌噬菌体重组酶，酿脓链球菌噬菌体重组酶，枯草芽孢杆菌噬菌体重组酶，结核分枝杆菌噬菌体重组酶和耻垢分枝杆菌噬菌体重组酶，条件是当第一重组连接位点是attB时，第二重组连接位点是attP，并且当第一重组连接位点是attP时，第二重组连接位点网站是attB。本发明还描述了用于产生转基因细胞，组织，植物和动物的组合物，载体及其使用方法。本发明的组合物，载体和方法也可用于基因治疗应用。

8. 发明授权

EP1844155B1 SITE-SPECIFIC SERINE RECOMBINASES AND METHODS OF THEIR USE 有权
标题翻译：工作的具体SERINREKOMBINASEN及其使用方法
公开(公告)号：EP1844155B1
公开(公告)日：2013-04-24
申请号：EP05722807.4
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： PADIDAM, Malla
IPC分类号： C12N9/00
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22

9. 发明公开

EP1844155A4 SITE-SPECIFIC SERINE RECOMBINASES AND METHODS OF THEIR USE 有权
标题翻译：维多利亚·维尔多瓦·多芬
公开(公告)号：EP1844155A4
公开(公告)日：2008-03-12
申请号：EP05722807
申请日：2005-02-08
申请人： INTREXON CORP
发明人： PADIDAM MALLA
IPC分类号： C12N15/85 , C07H21/04
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22
摘要： The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is attB, the second recombination attachment site is attP and when the first recombination attachment site is attP, the second recombination attachment site is attB. The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.
摘要翻译：本发明提供了一种在真核细胞中获得位点特异性重组的方法，所述方法包括提供包含第一重组附着位点和第二重组附着位点的真核细胞; 使第一和第二重组附着位点与原核重组酶多肽接触，导致重组附着位点之间的重组，其中重组酶多肽可以介导第一和第二重组附着位点之间的重组，第一重组附着位点是噬菌体基因组重组附着物位点（attP）或细菌基因组重组附着位点（attB），第二重组位点是attB或attP，重组酶选自单核细胞增生利斯特氏菌噬菌体重组酶，化脓性链球菌噬菌体重组酶，枯草芽孢杆菌噬菌体重组酶，结核分枝杆菌噬菌体重组酶和耻垢分枝杆菌噬菌体重组酶，条件是当第一重组附着位点为attB时，第二重组附着位点为attP，当第一重组附着位点为attP时，第二重组附着物 ent站点是attB。本发明还描述了用于产生转基因细胞，组织，植物和动物的组合物，载体及其使用方法。本发明的组合物，载体和方法也可用于基因治疗应用。

10. 发明公开

EP1844155A1 SITE-SPECIFIC SERINE RECOMBINASES AND METHODS OF THEIR USE 有权
标题翻译：工作的具体SERINREKOMBINASEN及其使用方法
公开(公告)号：EP1844155A1
公开(公告)日：2007-10-17
申请号：EP05722807.4
申请日：2005-02-08
申请人： Intrexon Corporation
发明人： PADIDAM, Malla
IPC分类号： C12N15/85 , C07H21/04
CPC分类号： C12N15/907 , C12N9/00 , C12N9/22 , C12N15/52 , C12N15/79 , C12Y301/22
摘要： The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is attB, the second recombination attachment site is attP and when the first recombination attachment site is attP, the second recombination attachment site is attB. The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.

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