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1. 发明授权

EP2010659B1 Kits and methods for generating 5' capped RNA 有权转让
标题翻译：试剂盒和方法用于生产RNA的5'-帽结构
公开(公告)号：EP2010659B1
公开(公告)日：2014-06-18
申请号：EP07775441.4
申请日：2007-04-16
申请人： CellScript, Inc.
发明人： JENDRISAK, Jerome , MEIS, Ronald , DAHL, Gary
IPC分类号： C12N15/11 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705

2. 发明公开

EP2010659A4 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 有权转让
标题翻译：套件，以及为生产RNA中的方法5'-结构封顶
公开(公告)号：EP2010659A4
公开(公告)日：2009-12-02
申请号：EP07775441
申请日：2007-04-16
申请人： EPICT TECHNOLOGIES
发明人： JENDRISAK JEROME , MEIS RONALD , DAHL GARY
IPC分类号： C12N15/11 , C12N15/113 , C12N15/85 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705

3. 发明公开

EP3303575A1 METHOD FOR ADDING CAP STRUCTURES TO RNA USING IMMOBILIZED ENZYMES 有权转让
标题翻译：使用固定化酶将帽结构添加到RNA的方法
公开(公告)号：EP3303575A1
公开(公告)日：2018-04-11
申请号：EP16727418.2
申请日：2016-05-30
申请人： CureVac AG
发明人： ROOS, Tilmann , YAZDAN PANAH, Benyamin , CONZELMANN, Markus , THESS, Andreas , BUOB, Dominik , KUNZE, Martin , WAGNER, Veronika
IPC分类号： C12N9/12 , C12N9/10 , C12N9/16 , C12N11/02
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N9/16 , C12N11/02 , C12N11/08 , C12Y201/01056 , C12Y201/01057 , C12Y207/0705 , C12Y301/03033
摘要： The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.

4. 发明公开

EP2010659A2 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 有权转让
标题翻译：试剂盒和方法用于生产RNA的5'-帽结构
公开(公告)号：EP2010659A2
公开(公告)日：2009-01-07
申请号：EP07775441.4
申请日：2007-04-16
申请人： Epicentre Technologies
发明人： JENDRISAK, Jerome , MEIS, Ronald , DAHL, Gary
IPC分类号： C12N15/11 , C12N15/85 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705
摘要： The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.

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